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Offlinehermandamerman
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Registered: 06/16/11
Posts: 69
Last seen: 13 years, 3 months
Super poor mans LC
    #14705332 - 07/02/11 03:52 AM (13 years, 6 months ago)

Ok so first off, my personal belief is the LC should be added to the PF TEC. Just my honest opinion. Beginners should be tought how to extend their 10cc syringes so that in the case they do fail at some point of the steril procedure, (or some other part of the Tec) they will still have lots of myc to try and try again until they get it right. I'm new to mycology, but have been doing other forms of growing, and I know the frustrations with failure. If you spend the time and money on buying spore syringes, and then making PF jars, and you use 10cc's on 10-12 jars, and you mess them up some how, that would be very discouraging.

With that said, here's my poor mans LC.

I took a bottle of bottled water. Just your run of the mill bottle of water. Put it in my glove box after spraying it down with Lysol, and spraying the air around it. I unscrewed the cap and poured aprox. 1 and 1/2 inches of the water out into a bowl. I then poured 1/2 teaspoon of organic honey into the bottle, squeezed the bottle to make sure there was plenty of room in the bottle for expansion of the contents and put the lid back tightly. I took the bottle out and gave it a good shake to ensure the honey was thoroughly mixed and I put the bottle in the microwave for one minute. I let the bottle sit in the microwave for 5 mins, and then cooked for one minute again.

I then took the bottle out with an oven mitt and placed it in a large bowl of ice water. Because the bottle is made of plastic, you do not have to worry about rapid decrease in temp shattering the bottle. Every few mins I would give the bottle a good shake or two in the ice water so as to move the water molecules around. This speeds up the cooling process because the water molecules closest to the inside will stay warmer than the ones touching the plastic. As soon as the bottle was no longer warm (not cold either) I sprayed down my glove box and air around it with Lysol and placed the bottle inside along with a lighter, alcohol swab, transpore tape, and spore syringe.

I flamed the needle, and swabbed the bottle cap. I then pierced the bottle cap with the syringe and needle, injected about 1/2 to 3/4 of a cc, and quickly removed and needle and placed a piece of transpore tap over the needle.

I then swirled the bottle (did not shake) and placed in a black plastic bag and put it in my incubator (at 72 deg F).
The next day I came back and gave it a good swirl. The day after came back and had myc growth colonizing the bottom 1/4 of the bottle. Swirled again.

So in less than 48 hours, I had great myc growth.

I would like to see if anyone else could try this method. I know it's not ideal, but was easy enough and didn't really cost me anything other than a spore syringe.

One thing I would recommend changing is the piercing straight through the cap. In the future, I will try making an RTV port in the cap first, because the bottle is not rigid like a glass jar, and every light squeez of the bottle makes for air exchange through the hole in the cap and that's an open invitation for contams. I don't think the transpore tape is enough to prevent that.

Let me know what you all think. I'm extremely open to criticism. Thanks for reading.

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Invisiblewildernessjunkie
Reshitivest
I'm a teapot


Registered: 06/13/10
Posts: 8,118
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Re: Super poor mans LC [Re: hermandamerman]
    #14705515 - 07/02/11 06:13 AM (13 years, 6 months ago)

Noob maneuver #1: Invent Tek that has already been invented.

Noob maneuver #2: Fail at already invented tek.

Noob maneuver #3: Swear by Tek

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InvisibleUnnamedGrower
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Registered: 04/17/11
Posts: 7,146
Loc: I'll be where I'm at
Re: Super poor mans LC [Re: wildernessjunkie]
    #14705522 - 07/02/11 06:18 AM (13 years, 6 months ago)

Quote:

wildernessjunkie said:
Noob maneuver #1: Invent Tek that has already been invented.

Noob maneuver #2: Fail at already invented tek.

Noob maneuver #3: Swear by Tek



:lmafo:

Yes you have mycellium growth in your bottle that doesnt mean that your LC is any good :shrug:  LC's are very very easy to fuck up.  My advice to you is to shot a test jar of said LC before you go and shoot 20 jars with it.

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Invisiblewildernessjunkie
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I'm a teapot


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: Super poor mans LC [Re: UnnamedGrower]
    #14705542 - 07/02/11 06:30 AM (13 years, 6 months ago)

Dont give up bro. Its all part of the learning process.

I just felt it was my duty here to give you shit.

Keep on keeping on.:wink:

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Offlinehermandamerman
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Registered: 06/16/11
Posts: 69
Last seen: 13 years, 3 months
Re: Super poor mans LC [Re: wildernessjunkie]
    #14705978 - 07/02/11 09:37 AM (13 years, 6 months ago)

Quote:

wildernessjunkie said:
Dont give up bro. Its all part of the learning process.

I just felt it was my duty here to give you shit.

Keep on keeping on.:wink:



Quote:

wildernessjunkie said:

Noob maneuver #1: Invent Tek that has already been invented.

Noob maneuver #2: Fail at already invented tek.

Noob maneuver #3: Swear by Tek




Butthole maneuver #1: be rude and act all knowing to a noob

Butthole maneuver #2: tell the noob he has failed miserably at something to make self feel better.

Butthole maneuver #3: say that the noob said something that they did not

Butthole maneuver #4: look like an ass then have a followup to make original post not look as bad

Lol ok so yea I may be a noob to the myc game, but in other grow games I'm not. So I know the importance of not getting mad over someones post. And I felt it was my duty as a noob to give you shit back all in the name of fun. Lol

Now I never said I swore by it. And the whole point of my post was to get feed back on whether it had been done before, or what people thought if it hadn't.  And I did say I'm open to criticism, lol just not dick headed ones. Haha

So next time instead of pontificating, try educating.
Why it's a failed Tec. Things like that. Thanks for reading my post and informing me. And know that I was just busting your balls back. I just had time on my hands, an idea, spore syringes, and no money till payday to get the proper supplies. Lol thanks again


--------------------
I am a Noob. Can't help it. Try and forgive me for any NewB mistakes or comments. Lol and if you would like to mentor me, PM me please.

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Offlinehermandamerman
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Last seen: 13 years, 3 months
Re: Super poor mans LC [Re: UnnamedGrower]
    #14706005 - 07/02/11 09:52 AM (13 years, 6 months ago)

Yea. I wasn't planning on doing a whole with this. Just wanted to get a proof of concept. Thanks for the advice. I have other LC's going that are done with more orthodox methods and I'm wanting to work with. Those too will be tested on a single jar though.

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InvisibleUnnamedGrower
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Registered: 04/17/11
Posts: 7,146
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Re: Super poor mans LC [Re: hermandamerman]
    #14706013 - 07/02/11 09:55 AM (13 years, 6 months ago)

This idea will work its just that LC's contam so easy you need to be super careful with them and doing shit like you posted to me is just begging for contams.

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OfflineRoastedPete
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Re: Super poor mans LC [Re: UnnamedGrower]
    #14706071 - 07/02/11 10:20 AM (13 years, 6 months ago)

Test jars. Someone said it already, but this is important.

I lost a fair amount of jars to contams from a botched LC. It was a sad  day.

you've got yourself a solid method if you can get it to work with out contamination problems. Good luck! :thumbup:

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Offlinehermandamerman
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Last seen: 13 years, 3 months
Re: Super poor mans LC [Re: RoastedPete]
    #14715790 - 07/04/11 01:23 PM (13 years, 6 months ago)

Hey. Thanks. The way I look at it, it's worth a try right? All I really waisted was 1cc of spores and some a BRF cake. Plus this is more like the Bear Grills way to get out of a jam. Lol


--------------------
I am a Noob. Can't help it. Try and forgive me for any NewB mistakes or comments. Lol and if you would like to mentor me, PM me please.

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