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Offlineironlion31386
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no pc experiment. you must believe!!
    #14702686 - 07/01/11 04:25 PM (13 years, 6 months ago)

soo i use my pc for every jar ive ever had grains inside of.. i will attempt to do a grain to grain transfer with a batch of non pc'd rye berries.. just simmered to perfection.. and be very careful not to get them to dirty when they are done.. when i do a graint to grain with a fully colonized jar to 7 jars of un colonized rye it take about 5 days to become colonized.. i personally dont think this is enough time for a contam to take hold... but anyways.. we will see.. i will start tomorrow with my soak..pics will be included with a walk through.  opinions anyone?  experiences?... is this experiment a waste of my time? LOL probably.  and this naturally happened, i promise...i know you think its amazing!

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Offlinethe astronaut
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Re: no pc experiment. you must believe!! [Re: ironlion31386]
    #14702793 - 07/01/11 04:50 PM (13 years, 6 months ago)

That pic is awsome but u really should at least boil the rye in a jar or somthing. But try it and lets see, good luck. :thumbup:

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Offlinediatom
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Re: no pc experiment. you must believe!! *DELETED* [Re: the astronaut]
    #14703122 - 07/01/11 05:51 PM (13 years, 6 months ago)

Post deleted by diatom

Reason for deletion: misread


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OfflineSomeGuy
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Re: no pc experiment. you must believe!! [Re: diatom]
    #14703154 - 07/01/11 05:56 PM (13 years, 6 months ago)

I think if you use a high ratio of colonized grain, it can be done. Boiling cripples or kills most bacteria

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Offlineironlion31386
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Re: no pc experiment. you must believe!! [Re: SomeGuy]
    #14711879 - 07/03/11 04:57 PM (13 years, 6 months ago)

only simmered. no pc rye berries. i am only doing one jar.. i felt that 7 would could be a waste of my time.. but an experiment none the less. i am only going to use 2 rounded table spoons of spawn. i will keep it sparate from my other jars incase it does contam.. i more concerend about there being to much water in there than i am contams.im put in in the fridge to wait 24hrs for the others to come out of the pc to do them all at the same time..

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InvisibleUnnamedGrower
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Re: no pc experiment. you must believe!! [Re: ironlion31386]
    #14711943 - 07/03/11 05:10 PM (13 years, 6 months ago)

:popcorn:

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OfflineSmirnoff
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Re: no pc experiment. you must believe!! [Re: ironlion31386]
    #14711958 - 07/03/11 05:13 PM (13 years, 6 months ago)

this pic is sick!

keep us posted... this experience is interesting...


--------------------
We found the mushroom forest, Let's meet under the cap!


Ethnobotany + Mycology = Ethnomycology

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Offlineveda_sticks
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Re: no pc experiment. you must believe!! [Re: Smirnoff]
    #14712114 - 07/03/11 05:59 PM (13 years, 6 months ago)

Interesting experoment though we pressure cook to kill bacterial endospores which survive steam sterilisation.

5 days would be plenty time for bacteria to atleast colinise a small portion of the substrate. which would make it useless for grain to grain transfers and i dont think it would be much use for bulk either.

alot of failed bulk grows have been the result of a hiding contam from spawn.


--------------------

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Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
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Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlineironlion31386
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Re: no pc experiment. you must believe!! [Re: veda_sticks]
    #14712196 - 07/03/11 06:19 PM (13 years, 6 months ago)

ok good point.. a couple important questions.. i have been going a little less with my pc lately.. only about 30mins at 15psi.. is this stupid for me to do?.. i keep hearing about these endo spores.. if my rye colonizes completely.. your saying there could be a contam in it still?.. i dont understand..i am only flushing my poo three times max.. i realy hate to be saying this but i havent seen any contams yet. i would hate to lose an entire tub over something like not pcing for the extra 15 minutes.

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InvisibleUnnamedGrower
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Re: no pc experiment. you must believe!! [Re: ironlion31386]
    #14712200 - 07/03/11 06:20 PM (13 years, 6 months ago)

You are suppose to PC grain for 1.5 hours and yes you could have hidden contams that will show up later to ruin your grow

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InvisibleShroomismM
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Re: no pc experiment. you must believe!! [Re: ironlion31386]
    #14712237 - 07/03/11 06:31 PM (13 years, 6 months ago)

Quote:

ironlion31386 said:
only simmered. no pc rye berries. i am only doing one jar.. i felt that 7 would could be a waste of my time..




IMO only 1 jar is a waste of your time.. not exactly a very conclusive test. No controls?
Good luck but I predict it probably won't end well, you very well may get lucky.. but there' a reason we PC whole grains.


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Offlineironlion31386
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Re: no pc experiment. you must believe!! [Re: Shroomism]
    #14712284 - 07/03/11 06:42 PM (13 years, 6 months ago)

LOL i hear you i have pcd like 150 jars sucessfully.. i know theres a reason silly. i just feel that in nature.. the ground isnt pressure cooked. i just wanted to try and see if i could succesfully colonize it, that is all. i guess if i were to be all natural, i would only soak and maybe pasturize

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Offlineironlion31386
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Re: no pc experiment. you must believe!! [Re: ironlion31386]
    #14712294 - 07/03/11 06:44 PM (13 years, 6 months ago)

but on the other hand my pinsets arent perfect. could that be something to do with hidden contams?..

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InvisibleShroomismM
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Re: no pc experiment. you must believe!! [Re: ironlion31386]
    #14712330 - 07/03/11 06:53 PM (13 years, 6 months ago)

Perfect pinsets come from perfect conditions. 100% colonization, proper moisture content, ideal fruiting conditions/humdity/FAE and light...
Also a strong strain is just as big of a factor. This is why an isolated strain will almost always produce a much better pinset than a multispore.
Hidden contams could potentially cause uneven pinsets but they are not even close to being a major factor in what makes a perfect pinset.


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Offlineironlion31386
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Re: no pc experiment. you must believe!! [Re: Shroomism]
    #14712351 - 07/03/11 06:58 PM (13 years, 6 months ago)

i want them soo bad. i already know my reason for not having nicer pinsets.. 1 no fan in my room 2 no temerature control 3 i have never used a hygrometer in any of my grows 4 i only use natural daylight 5 im a noob with monos. i definetly need to work on my FAE

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OfflineMasticore
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Re: no pc experiment. you must believe!! [Re: Shroomism]
    #14712698 - 07/03/11 08:25 PM (13 years, 6 months ago)

Quote:

Shroomism said:
Quote:

ironlion31386 said:
only simmered. no pc rye berries. i am only doing one jar.. i felt that 7 would could be a waste of my time..




IMO only 1 jar is a waste of your time.. not exactly a very conclusive test. No controls?
Good luck but I predict it probably won't end well, you very well may get lucky.. but there' a reason we PC whole grains.



Yup. One jar proves nothing, no matter how it ends up.


--------------------
Treat my posts like the Bible. I am fallible, subject to human limitation, and usually open to interpretation.

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Offlineironlion31386
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Re: no pc experiment. you must believe!! [Re: Masticore]
    #14712735 - 07/03/11 08:34 PM (13 years, 6 months ago)

jesus fucking christ.. forget about it! lol

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Offlineironlion31386
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Re: no pc experiment. you must believe!! [Re: ironlion31386]
    #14712757 - 07/03/11 08:39 PM (13 years, 6 months ago)

masticore.. i bet you play magik cards:tongue2:

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OfflineMasticore
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Re: no pc experiment. you must believe!! [Re: ironlion31386]
    #14712857 - 07/03/11 09:04 PM (13 years, 6 months ago)

Quote:

ironlion31386 said:
masticore.. i bet you play magik cards:tongue2:



No way.
:lolsy:



BTW, good luck either way. I'd hate to hear you wasted a jar of grain like this, but I've steam sterilized popcorn successfully so hopefully this will turn out well.


--------------------
Treat my posts like the Bible. I am fallible, subject to human limitation, and usually open to interpretation.

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Offlineironlion31386
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Re: no pc experiment. you must believe!! [Re: Masticore]
    #14713749 - 07/04/11 12:40 AM (13 years, 6 months ago)

o shit... thanks man... hey one more question.. if i have been pcing for only 30-45 mins up to this moment without any contams.. do you think i should change my method?. maybe up it to an hour? i guess i just dont fully believe in these incredibly heat tolerant endospores. hmmmmm

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Shop: Mushroom-Hut Substrate Bags   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


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