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InvisibleM11
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Registered: 06/14/09
Posts: 1,840
Sterile way to transfer sclerotia grown on agar to grain?
    #14691864 - 06/29/11 01:52 PM (13 years, 6 months ago)

Hey folks,

I am planning another truffle grow soon.  I think I am going to go with Mexicana A this time around instead of ATL 7. 

Anyway, I was wondering if it would be possible to grow some cultures out on agar, find the strains that are most likely to make stones, then transfer those strains to grain.  I would ideally like to make a grain LC out of this selected culture. 

Is there any sterile way to do this without a flow hood? 

M


--------------------
Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter.  Just pretend it's christmas.
-RR

Those mushrooms are fine.  Your friend is a pussy.
-RR

Outdoor Patch

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Offlinescatmanrav
Brainy Smurf


Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 12 years, 14 days
Trusted Cultivator
Re: Sterile way to transfer sclerotia grown on agar to grain? [Re: M11]
    #14692139 - 06/29/11 02:45 PM (13 years, 6 months ago)

I mist my bathroom with alcohol and spray with lysol after removing all towels/rugs. Then I have a clean bin with no lid on it that I turn on its side and set on my toilet. Just works as a shield to go over your work area. Then I put a peice of tin foil in front of your face where you sit as a breathing/sneeze shield. Poke a slit in it to see through. I do all G2G's like this. Put the colonized jar, or agar plate in the tub, all your jars next to you, then just pop them in the tub, pop a wedge, or spoonful in, then back out and next jar.

I've always done all G2G this way, thousands and thousands, rarely loose any.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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