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OfflineACID_noise
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Liquid Substrate Question
    #14691686 - 06/29/11 01:13 PM (13 years, 6 months ago)

So I currently have 12 freshly-picked wild cubes and I want to clone it using a liquid substrate (honey/water).  Once it fully colonizes it, can I begin fruiting in the liquid or do I have to transfer it to a solid medium?

If I have to transfer to a solid medium, can I use a dry cow patty?

Thanks for your support, people!


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OfflineRoastedPete
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Re: Liquid Substrate Question [Re: ACID_noise]
    #14691716 - 06/29/11 01:19 PM (13 years, 6 months ago)

I think what you are talking a about us a liquid culture.  You use those to colonize other substrates. If you manage to make a working culture, you can use it for any tek you see fit to try out. LC's take the place of spores.

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OfflineACID_noise
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Re: Liquid Substrate Question [Re: RoastedPete]
    #14691735 - 06/29/11 01:23 PM (13 years, 6 months ago)

thanks for your response

I see...so using liquid culture is an easier way to get a substrate colonized?

And what about cow patties? I live on a farm, and shrooms pop up on them occasionally, but as far as making a substrate from cow dung, what would you suggest?


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OfflineRoastedPete
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Re: Liquid Substrate Question [Re: ACID_noise]
    #14691773 - 06/29/11 01:32 PM (13 years, 6 months ago)

Yes, that is correct.

As for preparing substrates, there are plenty of teks on this website. Search around and see what you find!

And welcome!

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OfflineACID_noise
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Re: Liquid Substrate Question [Re: RoastedPete]
    #14691989 - 06/29/11 02:19 PM (13 years, 6 months ago)

thanks for the help i will def chek it out!


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InvisibleDoc_T
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Re: Liquid Substrate Question [Re: ACID_noise]
    #14692055 - 06/29/11 02:31 PM (13 years, 6 months ago)

You can NOT take a wild specimen and clone it to LC.


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OfflineWing
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Re: Liquid Substrate Question [Re: Doc_T]
    #14692099 - 06/29/11 02:37 PM (13 years, 6 months ago)

:whathesaid:

Well sorta ... You can make a LC and try it but it will most likely contaminate. Wild mushrooms are notoriously dirty. Your best bet is to get a spore print from one, transfer to agar and isolate it from the contaminates.

Or just buy a spore syringe and make a LC from that.


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Offlineoverstand
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Re: Liquid Substrate Question [Re: Wing]
    #14692790 - 06/29/11 04:56 PM (13 years, 6 months ago)

Cloning and fruiting wild mushrooms is complicated. It requires working with agar, strain isolation, pressure cooking, spawning to grain, casing and fruiting. The best bet for a noob is to buy a spore syringe and use the PF tek. That method is easy as pie. Here is a how to video on the PF tek. http://www.mushroomvideos.com/BRF-Pf-Tek


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InvisibleDoc_T
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Re: Liquid Substrate Question [Re: overstand]
    #14692810 - 06/29/11 05:00 PM (13 years, 6 months ago)

If you find a wild specimen, cloning to agar would be worth the work.
Steep learning curve but a great payout. And it's truly not all that hard.


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