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bholzer
quasi-scientist



Registered: 03/22/11
Posts: 2,409
Last seen: 12 years, 7 months
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Did I mess up bad?
#14691680 - 06/29/11 01:11 PM (13 years, 6 months ago) |
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I inoculated some jars about 3 days ago, but I'm worried I may have fucked up. I followed the "lets grow mushrooms" series exactly. The video didn't say anything about waiting after steam sterilization, so I only gave them about 15 minutes from steam to inoculation.
After reading, I've seen some people waiting up to 36 hours. Should I have done this? Did I fuck it up? Not seeing any colonization yet, but I wasn't really expecting to after only 3 days.
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Use these substances wisely, they have the ability to cause life altering realizations.
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overstand
Stranger



Registered: 02/05/11
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Re: Did I mess up bad? [Re: bholzer]
#14691695 - 06/29/11 01:15 PM (13 years, 6 months ago) |
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I am sorry but your spores are dead. They can not survive boiling temps. It is best to let the jars cool over night. 24 hours is ideal.
-------------------- “Disobedience is the true foundation of liberty. The obedient must be slaves.” - Henry David Thoreau
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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
Loc: @ Home
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Re: Did I mess up bad? [Re: bholzer]
#14691703 - 06/29/11 01:17 PM (13 years, 6 months ago) |
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Quote:
bholzer said: I inoculated some jars about 3 days ago, but I'm worried I may have fucked up. I followed the "lets grow mushrooms" series exactly. The video didn't say anything about waiting after steam sterilization, so I only gave them about 15 minutes from steam to inoculation.
After reading, I've seen some people waiting up to 36 hours. Should I have done this? Did I fuck it up? Not seeing any colonization yet, but I wasn't really expecting to after only 3 days.
hmmm.... thermal death of the spores will occure at 45°C/113°F.
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bholzer
quasi-scientist



Registered: 03/22/11
Posts: 2,409
Last seen: 12 years, 7 months
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Re: Did I mess up bad? [Re: overstand]
#14691704 - 06/29/11 01:17 PM (13 years, 6 months ago) |
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Quote:
overstand said: I am sorry but your spores are dead. They can not survive boiling temps. It is best to let the jars cool over night. 24 hours is ideal.
Ah bummer, no chance of survival there? The jars were warm to the touch, but not hot...
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Use these substances wisely, they have the ability to cause life altering realizations.
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bholzer
quasi-scientist



Registered: 03/22/11
Posts: 2,409
Last seen: 12 years, 7 months
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Re: Did I mess up bad? [Re: Luger0815]
#14691714 - 06/29/11 01:19 PM (13 years, 6 months ago) |
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Quote:
Luger0815 said:
hmmm.... thermal death of the spores will occure at 45°C/113°F.
Shit, I was probably flirting with about that temperature. I guess I'll give them a little bit to see, but now my hopes are crushed.
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Use these substances wisely, they have the ability to cause life altering realizations.
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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
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Re: Did I mess up bad? [Re: bholzer]
#14691727 - 06/29/11 01:21 PM (13 years, 6 months ago) |
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Quote:
bholzer said:
Quote:
Luger0815 said:
hmmm.... thermal death of the spores will occure at 45°C/113°F.
Shit, I was probably flirting with about that temperature. I guess I'll give them a little bit to see, but now my hopes are crushed.
If you have some spores left in the syringe, just innoc. again.
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bholzer
quasi-scientist



Registered: 03/22/11
Posts: 2,409
Last seen: 12 years, 7 months
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Re: Did I mess up bad? [Re: Luger0815]
#14691738 - 06/29/11 01:23 PM (13 years, 6 months ago) |
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Quote:
Luger0815 said:
Quote:
bholzer said:
Quote:
Luger0815 said:
hmmm.... thermal death of the spores will occure at 45°C/113°F.
Shit, I was probably flirting with about that temperature. I guess I'll give them a little bit to see, but now my hopes are crushed.
If you have some spores left in the syringe, just innoc. again.
I don't have any left in that syringe, but I do have another syringe that I suppose I could use. Would I be okay just to move my jars back to my still-air box and re-inoc? Or should I sterilize them again?
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Use these substances wisely, they have the ability to cause life altering realizations.
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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
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Re: Did I mess up bad? [Re: bholzer]
#14691759 - 06/29/11 01:28 PM (13 years, 6 months ago) |
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You could innoc with the other syringe, wait or sterilize and innoc again. Your choice. 3 days is not enough time to see any germination. Maybe you should wait another week, to see if the spores survived. I would start over. Good luck....
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nixon
Funguy



Registered: 11/26/10
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Re: Did I mess up bad? [Re: Luger0815]
#14691852 - 06/29/11 01:51 PM (13 years, 6 months ago) |
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In my grow, i only gave the jars about 45 min to cool, the spores are colonizing like crazy now so you might still have a chance, give it approx. 6 days and if no jars started colonizing then start over.
-------------------- masspan said:
in your 2nd pic that mushroom is actively standing on its head for sure
nearhorn said:
what does that mean? its active?
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