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Offlineshroomchef
Chef

Registered: 03/30/03
Posts: 74
Loc: Somewhere in the Northeas...
Last seen: 13 years, 10 months
Pre-steril bags How can you tell if they are contaminated?
    #1467178 - 04/17/03 02:17 PM (15 years, 1 month ago)

How can you tell if those presterilized bags with filter patch and rubber injection site are contaminated without ripping them open?

Specifically, I bought four and inoculated them w/ maz; two weeks later one showed growth but then appeared to harden and die it seemed. So i crumbled it inside bag and reinoculated and now the one is showing good growth; however no growth has been seen from the other three I have even reinoculated two weeks ago and still no visible signs so i'm wondering if their no good? Spores are fine grown 9 pf cakes with syringe no prob conditions were extremely sterile.


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What kind of room can you not go into because it has no walls,windows, or doors?

What's the beginning of eternity,
the end of time and space,
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OfflineBig_D
Baaith AKA LoneSoldier
Registered: 04/11/03
Posts: 50
Last seen: 12 years, 8 months
Re: Pre-steril bags How can you tell if they are contaminate [Re: shroomchef]
    #1467345 - 04/17/03 03:17 PM (15 years, 1 month ago)

If you used the same syringe to innoculate the cakes and bags but the cakes are showing growth, and the bags aren't... Then it is the bags not the spores. Hence, you have bad bags. Whoever you got it from should replace it with no problem.

But if they try to tell you that its your spores, then they are incorrect (assuming that it happened they way I enterpreted it above)

Peacy out Dun,


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Leave Me Alone!


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Anonymous

Re: Pre-steril bags How can you tell if they are contaminated? [Re: shroomchef]
    #1467349 - 04/17/03 03:19 PM (15 years, 1 month ago)

when you inoculated these bags, did you flame-sterilize the needle and swab the inoculation point with disinfectant first?


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Offlineshroomchef
Chef

Registered: 03/30/03
Posts: 74
Loc: Somewhere in the Northeas...
Last seen: 13 years, 10 months
Re: Pre-steril bags How can you tell if they are contaminated? [Re: ]
    #1467396 - 04/17/03 03:37 PM (15 years, 1 month ago)

yeah i swabbed and flamed sterilized; lysol entire work area, no drafts, showered, clean clothes, dust mask, covered hai, gloves, just about everything but sterilized glove box. Yeah its probably just the bags however one opf them was fine it was the same syringe thxs anyway for the advice.


--------------------
What kind of room can you not go into because it has no walls,windows, or doors?

What's the beginning of eternity,
the end of time and space,
the beginning of the end,
and the end of every place?




Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Pre-steril bags How can you tell if they are contaminated? [Re: shroomchef]
    #1467404 - 04/17/03 03:40 PM (15 years, 1 month ago)

email the vendor and ask for a replacement. remember not to tell them that you were using their product to commit a crime though.


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