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 Gigabyt3r
Stranger
Registered: 05/24/11
Posts: 58
Last seen: 11 years, 28 days
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 Is mycelium really that sensitive to contamination? 
#14658177 - 06/23/11 08:57 AM (11 years, 1 month ago) |
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How come it only takes 2 minutes to kill all bacteria with boiling water but Aall the guides on here say to PC for 60mins on average?
Why so long?
Also, I was wondering, I bought a spore syringe, and accidently pulled it so it sucked air in. Are they really that sensitive that now the syringe is now contaminated since it sucked some 'normal' air in?
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"Ummm, you have to be a run-of-the-mill dipshit to use popcorn as your substrate, and sterilize it in the microwave"
<3 Shiitake's
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SomeGuy
I feel better now :)
 
Registered: 04/18/10
Posts: 7,496
Loc: 
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Re: Is mycelium really that sensitive to contamination? [Re: Gigabyt3r]
#14658193 - 06/23/11 09:08 AM (11 years, 1 month ago) |
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Because it takes that long for the heat to penetrate the jar, and through the substrate to the center. Some bacteria die in minutes, but some takes longer. I don't have the paper in front of me, but I think the thermal death time for psuedomonas tolasii is 13 minutes, at 250f(which is 15psi) and so it would have to have heated a long time to get to that temp. in the center of the jar. You'l just have to try the syringe to find out, but yea, it's not a good thing to suck atmospheric air into a syringe
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 virus1824
Mr Mushroom
Registered: 09/25/05
Posts: 1,751
Loc: Europe
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Re: Is mycelium really that sensitive to contamination? [Re: SomeGuy]
#14658201 - 06/23/11 09:11 AM (11 years, 1 month ago) |
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Quote:
Is mycelium really that sensitive to contamination?
I think what you ment to say was: ïs substrate that sensitive to contamination.
And yes, some bacteria even survive the 90 minute sterilizing process.
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A weekend wasted is never a wasted weekend
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Gigabyt3r
Stranger
Registered: 05/24/11
Posts: 58
Last seen: 11 years, 28 days
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Re: Is mycelium really that sensitive to contamination? [Re: SomeGuy]
#14658210 - 06/23/11 09:17 AM (11 years, 1 month ago) |
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Quote:
paducahovoids said:
Because it takes that long for the heat to penetrate the jar, and through the substrate to the center. Some bacteria die in minutes, but some takes longer. I don't have the paper in front of me, but I think the thermal death time for psuedomonas tolasii is 13 minutes, at 250f(which is 15psi) and so it would have to have heated a long time to get to that temp. in the center of the jar. You'l just have to try the syringe to find out, but yea, it's not a good thing to suck atmospheric air into a syringe
Thanks for that reply, I understand now. I hope my syringe is ok ... 
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"Ummm, you have to be a run-of-the-mill dipshit to use popcorn as your substrate, and sterilize it in the microwave"
<3 Shiitake's
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ozzysmygod
Late Night PC'ing Enthusiast
Registered: 11/26/08
Posts: 835
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Re: Is mycelium really that sensitive to contamination? [Re: Gigabyt3r]
#14658454 - 06/23/11 11:01 AM (11 years, 1 month ago) |
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Your syringe is probably fooked...
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Vartousky
Stranger
Registered: 04/23/11
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Re: Is mycelium really that sensitive to contamination? [Re: ozzysmygod]
#14659099 - 06/23/11 01:45 PM (11 years, 1 month ago) |
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The mycelium can take over contaminants if they have advantages. You'll be fine, I've experimented with worse sterile procedure that worked. I'm not saying you should add air contaminants to your syringes, but it should be fine. It really demands though, I've seen mycelium eat trichoderma and vise versa.
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k00laid
NEMO
Registered: 05/03/10
Posts: 19,625
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Re: Is mycelium really that sensitive to contamination? [Re: Vartousky]
#14659865 - 06/23/11 03:36 PM (11 years, 1 month ago) |
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Quote:
Vartousky said:
It really demands though, I've seen mycelium eat trichoderma and vise versa.
no you havent.
trichoderma grows ON the cubensis mycelium, it cannot be beaten. it might conceal the trichoderma for a little while. but thats all
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AMU - AMU Q & A - MyVideo Teks!
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diatom
Strangler
Registered: 01/08/11
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Re: Is mycelium really that sensitive to contamination? [Re: k00laid]
#14659934 - 06/23/11 03:47 PM (11 years, 1 month ago) |
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I've sucked air into syringes before and I didn't have any contams so you'll probably be ok as far as the syringe goes.
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Wing
The Eye Tyrant
Registered: 04/26/11
Posts: 3,293
Last seen: 11 months, 11 days
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Re: Is mycelium really that sensitive to contamination? [Re: k00laid]
#14659955 - 06/23/11 03:50 PM (11 years, 1 month ago) |
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Quote:
k00laid said:
Quote:
Vartousky said:
It really demands though, I've seen mycelium eat trichoderma and vise versa.
no you havent.
trichoderma grows ON the cubensis mycelium, it cannot be beaten. it might
There is only a few contams that can be treated and gotten rid of. Trich is not one of them.
Edited by Wing (06/23/11 03:57 PM)
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maug
Registered: 11/28/10
Posts: 1,703
Loc: inside you
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Re: Is mycelium really that sensitive to contamination? [Re: Wing]
#14660134 - 06/23/11 04:12 PM (11 years, 1 month ago) |
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You're not killing all the bacteria when you pasteurize. I pasteurized a few of jars, and I ended up just letting 6 of them sit around uninoculated. A month or so later, they had some healthy bacteria colonies on them, and it looked like some trich that was about to turn green. They smelled like fruity oat bran, with a hint of leather.
Cube myc is generally pretty tough though. They will eat and fight the bacteria, but it will slow down colonization time.
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I think nighttime is dark so you can imagine your fears with less distraction. -Calvin and Hobbes
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PrimalSoup
illuminations
Registered: 11/17/09
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Loc: PNW
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Re: Is mycelium really that sensitive to contamination? [Re: maug]
#14660323 - 06/23/11 04:37 PM (11 years, 1 month ago) |
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Sterilized jars will keep a long time if you don't have any open contamination pathways. Sitting like this they stay good for weeks on end - but that's just how slow I am sometimes. 
@OP: Healthy fully colonized myc is resistant to contamination. But put competitor/parasite spores or endospores on the same substrate with a myc colony and let them fight it out for growth space? Not so good. Trich sucks big time.
 PS
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects:  Wheat Prep 2.0 Acidic Tea Tek Potency Project!
Edited by PrimalSoup (06/23/11 04:51 PM)
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