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VaeVictum
Sativa Cyborg

Registered: 06/01/11
Posts: 4,397
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I bought two syringes from a sponsor of the forums and have a question. *DELETED*
#14622686 - 06/16/11 01:27 PM (12 years, 8 months ago) |
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Post deleted by VaeVictumReason for deletion: revising
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Jeffreylabowski
THE DUDE


Registered: 02/28/11
Posts: 90
Loc: midwest
Last seen: 12 years, 7 months
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Re: I bought two syringes from a sponsor of the forums and have a question. [Re: VaeVictum]
#14622718 - 06/16/11 01:32 PM (12 years, 8 months ago) |
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what was your method of sterilyzation? Some houses can be full of trich spores. How well did you clean the room where your storing your jars?
It could be a bad syringe but i have never bought anything from lilshopofspores.. If its a sponsor then I would assume the syringe is good but you never know.. Thats why i like making my own
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: I bought two syringes from a sponsor of the forums and have a question. [Re: VaeVictum]
#14622722 - 06/16/11 01:32 PM (12 years, 8 months ago) |
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Put some of the cambo syringe on agar & clean it up if it's dirty.
Did you inoculate in an SAB/GB?
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Crushed
Sober Enough


Registered: 02/18/10
Posts: 96
Loc: Southwest
Last seen: 12 years, 6 months
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Re: I bought two syringes from a sponsor of the forums and have a question. [Re: TranscendingLife]
#14622743 - 06/16/11 01:37 PM (12 years, 8 months ago) |
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It is possible the syringe is bad, it does happen. My guess would be though that the needle got contaminated at some point during the inoculation process. Did you flame sterilize between jars?
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In theory, there is no difference between theory and practice. In practice, there is.
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VaeVictum
Sativa Cyborg

Registered: 06/01/11
Posts: 4,397
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Re: I bought two syringes from a sponsor of the forums and have a question. [Re: TranscendingLife]
#14622764 - 06/16/11 01:40 PM (12 years, 8 months ago) |
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I sterilized by using 2 lighters together and wiped with alcohol after. I didn't have a glovebox or sab I have an island in the middle of my kitchen which is really big and inoculated there in still air.
Like I said It's just fucked up because the panama strain I didn't even sterilize while inoculating and they are all healthy and trich free.
Whereas the cambodia which I was MORE cautious with is giving me nothing but yucky jars!
I have 4 cc's left if I can get an agar plate what would my process be? Let it grow then cut out healthy myc and try to get no trich then drop it on top of a jar?
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VaeVictum
Sativa Cyborg

Registered: 06/01/11
Posts: 4,397
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Re: I bought two syringes from a sponsor of the forums and have a question. [Re: VaeVictum]
#14622770 - 06/16/11 01:41 PM (12 years, 8 months ago) |
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Yes I said in my first post that I sterilized in between every jar after I used all 10 cc's of my panama which is growing fine.
The cambodia is giving me nothing but trich and I sterilized it an infinite amount times more than the other.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: I bought two syringes from a sponsor of the forums and have a question. [Re: VaeVictum]
#14622786 - 06/16/11 01:43 PM (12 years, 8 months ago) |
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your kitchen & your bathroom are the two dirtiest places in your house.
You could cut a section of healthy myc out & drop it in a jar, but you'd need something other than a BRF jar for it as you'd just be dropping it on the dry verm layer.
You can make sorta a slurry outta it to transform it somewhat into an LC.
Just do that once you KNOW you don't have any trich in the agar dish.
Also, I'd recommend investing in an alcohol lamp, or making your own w/ a 1/2 pint jar & a shoe lace.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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chal420
plur


Registered: 04/02/11
Posts: 238
Last seen: 12 years, 6 months
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Re: I bought two syringes from a sponsor of the forums and have a question. [Re: VaeVictum]
#14622800 - 06/16/11 01:47 PM (12 years, 8 months ago) |
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get some lysol and spray the air and the table where you inject let sit for 10 15 min and do it again try and kill all the airborn bac. and molds
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: I bought two syringes from a sponsor of the forums and have a question. [Re: chal420]
#14622807 - 06/16/11 01:48 PM (12 years, 8 months ago) |
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lysol does not sanitize the air. It's a surface disinfectant.
Oust will disinfect the air.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Jeffreylabowski
THE DUDE


Registered: 02/28/11
Posts: 90
Loc: midwest
Last seen: 12 years, 7 months
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Re: I bought two syringes from a sponsor of the forums and have a question. [Re: TranscendingLife]
#14622823 - 06/16/11 01:51 PM (12 years, 8 months ago) |
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by sterilyzing i meant did you PC your jars? For how long? Did you open your PC when it was still hot?
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chal420
plur


Registered: 04/02/11
Posts: 238
Last seen: 12 years, 6 months
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Re: I bought two syringes from a sponsor of the forums and have a question. [Re: TranscendingLife]
#14622831 - 06/16/11 01:53 PM (12 years, 8 months ago) |
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Quote:
todlow said:
Also, I'd recommend investing in an alcohol lamp, or making your own w/ a 1/2 pint jar & a shoe lace.
dude awesome i never thought about doing that great tip ill have to try that sometime. does a shoe lace work really well? it does not burn up too quick?
also they have air disinfectant wich is what i got it kills 99.9 percent of airborn bac and mold or you can make a bleach solution right?
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: I bought two syringes from a sponsor of the forums and have a question. [Re: chal420]
#14622860 - 06/16/11 01:58 PM (12 years, 8 months ago) |
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The shoelace won't burn up, because it will be soaked w/ denature alcohol. Just UTSF & there's a tek up for it somewhere.
there is the Lysol w/ air disinfectant, yes.
1:10 bleach to water is what I use. Works great.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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