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OfflineBaby_Hitler
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2 quarts of L. edodes on popcorn, what should I do with it?
    #1461981 - 04/15/03 11:07 PM (14 years, 10 months ago)



I assume doing a block would be faster than making dowells and hammering them into logs later this year. I'd rather do that really, maybe both. Does anybody have a good link to how to do blocks?

These jars were innoculated like 2 months ago. They're starting to get brown spots. What does that mean?


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[quote]RogerRabbit said:
Actually, it's very easy to isolate a super producing sclerotia strain.

Follow the strain isolation technique on Let's Grow Mushrooms, and then select sectors early which are brown in color.  By the second or third transfer, you'll see stones developing, and this is only about 1 month after the original swipe of spores on agar.

Now, take each stone and move it to a new dish.  Soon, the mycelium will grow out and you'll see fresh stones developing, and if they're good strains, the sclerotia is forming long before the mycelium reaches the edge of the plate.  Pick strains which form four or five stones within two weeks and use these for your grain masters.
RR [/quote]


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Offliner05c03
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Re: 2 quarts of L. edodes on popcorn, what should I do with it? [Re: Baby_Hitler]
    #1462004 - 04/15/03 11:12 PM (14 years, 10 months ago)

2 months is kind of a long time to keep spawn. The brown spots may be age related, a sign of being a little past their prime. If you can get the indoor parameters right blocks would be cool, but logs are nice and low tech and you just need to be able to keep the logs moist enough and let nature take its course. You have plenty of material to do both.


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OfflineBaby_Hitler
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Re: 2 quarts of L. edodes on popcorn, what should I do with it? [Re: r05c03]
    #1462045 - 04/15/03 11:24 PM (14 years, 10 months ago)

I have a huge stack of split oak firewood. It was cut about 2 years ago, and has been sitting in a stack outside that whole time.

No logs though, just wedges about 10-12 inches long.


--------------------
[quote]RogerRabbit said:
Actually, it's very easy to isolate a super producing sclerotia strain.

Follow the strain isolation technique on Let's Grow Mushrooms, and then select sectors early which are brown in color.  By the second or third transfer, you'll see stones developing, and this is only about 1 month after the original swipe of spores on agar.

Now, take each stone and move it to a new dish.  Soon, the mycelium will grow out and you'll see fresh stones developing, and if they're good strains, the sclerotia is forming long before the mycelium reaches the edge of the plate.  Pick strains which form four or five stones within two weeks and use these for your grain masters.
RR [/quote]


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Offlinezeronio
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Re: 2 quarts of L. edodes on popcorn, what should I do with [Re: Baby_Hitler]
    #1462727 - 04/16/03 02:43 AM (14 years, 10 months ago)

The brown spots are normal with shiitake. When you make blocks you're supposed to wait until the white mycellium turns yellow and brown before you put it to fruiting chamber.
The blocks are usually made from a mixture of sawdust, wood chips and up to 10% of supplement (rice bran, oat meal, ...) and gypsum. I fruited them also from straight sawdust but I got only few small fruits.


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Offlinezeronio
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Re: 2 quarts of L. edodes on popcorn, what should I do with [Re: Baby_Hitler]
    #1462742 - 04/16/03 02:49 AM (14 years, 10 months ago)

Quote:

I have a huge stack of split oak firewood. It was cut about 2 years ago, and has been sitting in a stack outside that whole time.

No logs though, just wedges about 10-12 inches long.




I have got similar wedges (wood bricks). I'm preparing sawdust spawn for various species and then I'm going to stack soaked wood bricks and put spawn between layers.
I hope to get a big mushroom wall this way. :tongue: 


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Re: 2 quarts of L. edodes on popcorn, what should I do with [Re: zeronio]
    #1462753 - 04/16/03 02:53 AM (14 years, 10 months ago)

My first attempt with wood turned green. What kind of container should I use, and what steps should I take towards sterility when spawning to wood?


--------------------
[quote]RogerRabbit said:
Actually, it's very easy to isolate a super producing sclerotia strain.

Follow the strain isolation technique on Let's Grow Mushrooms, and then select sectors early which are brown in color.  By the second or third transfer, you'll see stones developing, and this is only about 1 month after the original swipe of spores on agar.

Now, take each stone and move it to a new dish.  Soon, the mycelium will grow out and you'll see fresh stones developing, and if they're good strains, the sclerotia is forming long before the mycelium reaches the edge of the plate.  Pick strains which form four or five stones within two weeks and use these for your grain masters.
RR [/quote]


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Offlinezeronio
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Re: 2 quarts of L. edodes on popcorn, what should I do with [Re: Baby_Hitler]
    #1462884 - 04/16/03 04:30 AM (14 years, 10 months ago)

I use polypropylene bags with Tyvek filter patch. IME you have to sterilize it longer becuase of slower heat penetration or preboil it before sterilization. Try also to get very fresh sawdust and chips - it has less resident contamns. I had problems with forest green mold when I used sawdust that was lying outside for some time.
That's for preparing spawn - you don't have to sterilize wood logs or bricks for outdoor growing.


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