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Invisiblearis
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What are your thoughts about Test Tubes?
    #14310639 - 04/18/11 07:00 AM (12 years, 10 months ago)



I found RR's DVD and lipa's stock culture tek helpful.
I was lucky enough to find a lot of pyrex at a tag sale a few years ago!

I have about 70 small tubes
12 of the medium
9 of the largest

How would you use these different size tubes?


Edited by aris (04/18/11 11:58 AM)


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InvisibleDoc_T
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Re: What are your thoughts about Test Tubes? [Re: aris]
    #14310647 - 04/18/11 07:03 AM (12 years, 10 months ago)

Large = 25mm?


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Offlinedankakanat
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Re: What are your thoughts about Test Tubes? [Re: Doc_T]
    #14310994 - 04/18/11 08:56 AM (12 years, 10 months ago)

do they sell test tubes at normale stores besides internet i cant seem to find them?


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OfflineRogerRabbitM
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Re: What are your thoughts about Test Tubes? [Re: dankakanat]
    #14311125 - 04/18/11 09:43 AM (12 years, 10 months ago)

To find test tubes locally, go to your city's hospital district and find a medical supply house.  They'll either have or can order test tubes and petri dishes.

You would treat larger tubes just as smaller ones except for the amount of agar and the size of the piece of wood you put in each.
RR


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Re: What are your thoughts about Test Tubes? [Re: dankakanat]
    #14311130 - 04/18/11 09:43 AM (12 years, 10 months ago)

Lab supply places have them, but they tend to be hard to fine locally IME.

aris, they are all screw cap yes? Screw tops are the best. You *can* do it with straight mouth ones and stick a piece of polyfil in covered with foil, buuuuuut one wrong move and that will fall out in not sterile conditions. I test tube bung may provide better results, but you'll need to figure out some way of providing a little gas exchange.

All the sizes should all work the same and be used the same per RR's tek on the video. I use large ones for my culture storage because I found some super cheap on ebay. I like them because they are easy to get culture pieces out of, but the small ones will work just fine provided you have small enough tools... which shouldn't be an issue.


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“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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InvisiblePrisoner#1
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Re: What are your thoughts about Test Tubes? [Re: PitcherCrab]
    #14311539 - 04/18/11 11:43 AM (12 years, 10 months ago)

Quote:

PitcherCrab said:
Screw tops are the best. You *can* do it with straight mouth ones and stick a piece of polyfil in covered with foil.




they also sell slip on caps to cover test tubes




search for polypropylene  test tube closure or test tube caps


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Invisiblearis
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Storage and sealing of Test Tubes? Refrigerator? [Re: PitcherCrab]
    #14311585 - 04/18/11 11:55 AM (12 years, 10 months ago)

Thanks for the tips everyone!

I wish the test tubes had screw caps but alas, they do not.  At least they were almost free! 


Whats the best temperature to aim for in the stock refrigerator?

How often do I have to let some fresh air into my stock refrigerator? 

Does light influence long term storage of cultures?

Is it ever best to freeze a culture? 

When I have a minute I will add insulation to my mini-frig maybe.


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InvisiblePrisoner#1
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Re: Storage and sealing of Test Tubes? Refrigerator? [Re: aris]
    #14311602 - 04/18/11 12:00 PM (12 years, 10 months ago)

for storage you want below 0F but you want the temp to be steady like a deep
freezer, a refridgerator/freezer cycles on and off which can destroy the
culture. you dont need to provide any air exchange because the culture will
be dormant, I've used distilled water and mineral oil for topping my slants
distilled water is preferable


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OfflineRogerRabbitM
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Re: Storage and sealing of Test Tubes? Refrigerator? [Re: Prisoner#1]
    #14312367 - 04/18/11 02:13 PM (12 years, 10 months ago)

Many species will be killed by storage below 0C/32F.  Other species such as Shiitake and oyster can be readily frozen.  One needs to be sure first.  I keep my culture refrigerator at 1C/34F.  The slants are all in insulated lunch boxes to ameliorate the temperature swings.
RR


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Invisiblemaug
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Re: Storage and sealing of Test Tubes? Refrigerator? [Re: RogerRabbit]
    #14312537 - 04/18/11 02:36 PM (12 years, 10 months ago)

I saw another user post this link when they were talking about how they make their self-sealing lids (they used the blue rubber stoppers). They also have plastic tubes, and those test tube lids that were mentioned. Even if privacy were an issue, I think I would prefer to buy from a place like this, and maybe have it shipped to a middle man. Hope that helps.


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Re: Storage and sealing of Test Tubes? Refrigerator? [Re: RogerRabbit]
    #14314588 - 04/18/11 11:01 PM (12 years, 10 months ago)

Quote:

RogerRabbit said:
Many species will be killed by storage below 0C/32F.  Other species such as Shiitake and oyster can be readily frozen.  One needs to be sure first.  I keep my culture refrigerator at 1C/34F.  The slants are all in insulated lunch boxes to ameliorate the temperature swings.
RR




Hmmm... I have had quite a few cultures destroyed by the fluctuation of my mini fridge. The insulated lunch boxes are a great solution! RR, what sort of lunch boxes are the ones you use? Are they the soft ones?


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PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
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“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Invisiblearis
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Re: Storage Tubes /w distilled h2o? [Re: PitcherCrab]
    #14616683 - 06/15/11 10:13 AM (12 years, 8 months ago)

Quote:

PitcherCrab said:
Hmmm... I have had quite a few cultures destroyed by the fluctuation of my mini fridge. The insulated lunch boxes are a great solution!




I found some on sale that had a removable rigid interior.  All plastic..


What is the advantage of storage on agar, instead of suspension in distilled water? *

An original goal here was to fit more samples in a smaller amount of refrigerated space.  I have no idea how fast a para filmed test tube, full of h20 (rather than agar) evaporates at 34F (or room temp for that matter). 

I'm reading that the distilled water storage doesn't even require refrigeration.

I would like to waste as little space and distilled water as possible.  Too small?

 

http://www.shroomery.org/forums/showflat.php/Number/14632590#14632590


*
Quote:

One Reference that appeared in Applied Microbiology, Aug. 1974,
by M.R.McGinnis, A.A.Padhye, and L.Ajello described the tests
that were performed. "Four hundred and seventeen isolates of 147
species belonging to 66 genera of filamentous fungi, yeasts, and
aerobic actinomycetes were maintained in sterile distilled water at
room temperature over periods ranging from 12 to 60 months in
four independent experiments. Of the 417 cultures, 389 (93%)
survived storage."

------------------------------


Of the 151 isolates taken from water storage (after
storage up to 7 yr), 94% were viable. For those isolates
with previously recorded growth rates, the growth rates
were not consistently affected by the distilled water
storage method (TABLE I). For those for which previous
information was not available, the growth rates
were as expected (TABLE I). Thus, water storage did
not have an obvious influence on culture viability or
growth rate.

-->
Preserving cultures of wood-decaying Badidiomycotina using sterile distilled water in cryovials
http://www.fpl.fs.fed.us/documnts/pdf1994/burds94a.pdf





Edited by aris (06/18/11 11:03 AM)


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Offlinetotal
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Re: Storage Tubes /w distilled h2o? [Re: aris]
    #14616704 - 06/15/11 10:18 AM (12 years, 8 months ago)

I've been wanting to jump on the cyrovial bandwagon myself...
They are pretty close in price to glass tubes, and can be autoclaved just like'em...
Inside threads...
DNA and Genetic info free...

Sounds like the perfect storage...

And 5 years is a LONG time lipa!


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Offlinetotal
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Re: Storage Tubes /w distilled h2o? [Re: total]
    #14616728 - 06/15/11 10:22 AM (12 years, 8 months ago)

Lipa...
Id like to take a moment to thank you for that research paper btw!
It was a great read...
Now im getting me some Cyrotubes for sure...


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