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OfflineMad_Hatter2004
Surfista Amigo
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Registered: 06/19/04
Posts: 2,298
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Re: Karo and sterilization [Re: ozzysmygod]
    #14611497 - 06/14/11 11:17 AM (12 years, 8 months ago)

Quote:

ozzysmygod said:
Here is just one-

Quote:

RogerRabbit said:
If you flame sterilize, anything you do afterward will only make the needle 'dirtier'.  Why not just flame and use?  Forget the alcohol after flaming.  There is no need to cool down the needle.  Just use hot and let the first drop or two of solution cool the needle, so the rest can flow contaminant free.
RR




I have seen hundreds of posts in hundreds of threads from trusted cultivators  stating that wiping anything down with alcohol after flame sterilization only serves to make them more dirty. Ive seen a thread where RR says he's been trying for years to tell people to stop doing it, but people carry on anyway.

Alcohol is a sanitizer not a sterilizer. I might even make a spore syringe using 70% isopropyl alcohol to prove its ineffectivness at killing spores.

From wikipedia "As an antiseptic it is good against vegetative bacteria and fair against fungi and viruses, but is ineffective against spores."

...





Not in any way shape or form trying to discredit RR (he's the man) but over the years I have seen him say one thing only to turn around and say the complete opposite at another time.

I'll continue to flame sterilize and then wipe with a clean paper towel soaked in isopropyl alcohol.Also,if you're going to quote someone,you might want to add what date that quote is taken from.


--------------------
7 days without waves makes one weak!

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Offlineozzysmygod
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Re: Karo and sterilization [Re: Mad_Hatter2004]
    #14611534 - 06/14/11 11:26 AM (12 years, 8 months ago)

The quote was taken from a thread in 07 but I've seen RR telling people not to wipe down syringes with alcohol this week. That was just one of the first search results.

He does sometimes update his methods, where research and experimentation proves old methods ineffective. But no matter how much research and experimentation you carry out alcohol, isopropyl or otherwise, isnt just going to miraculously start destroying spores.

Wikipedia says in black and white that rubbing alcohol does not kill spores, which you have ignored in your last post.

I will form a thread when I have time listing the hundreds of quotes and sources stating that wiping needles down with alcohol is ineffective.

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Offlinetotal
Post Office Tyvek Advocator
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Registered: 10/03/04
Posts: 11,406
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Re: Karo and sterilization [Re: ozzysmygod]
    #14611593 - 06/14/11 11:38 AM (12 years, 8 months ago)

You guys need to find out whats right for you...
I have done both, both wipe and not...
They both work... :super:

BUT...
Now i just red hot a needle and shove it in there...
The logic is that, if its red hot, everything is dead and there is no need for the alcohol(other than cooling off the syringe so you dont waste a small amount of spores/lc to cool it)

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OfflineMad_Hatter2004
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Re: Karo and sterilization [Re: ozzysmygod]
    #14611605 - 06/14/11 11:41 AM (12 years, 8 months ago)

I never said it would kill contaminat mold spores,but it is an effective extra precautionary step IMHO.Hell it's even still shown on the lets grow mushrooms page.


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Offlineozzysmygod
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Registered: 11/26/08
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Re: Karo and sterilization [Re: Mad_Hatter2004]
    #14611660 - 06/14/11 11:53 AM (12 years, 8 months ago)

Quote:

ozzysmygod said:
From wikipedia "is ineffective against spores."





Quote:

Mad_Hatter2004 said:it is an effective extra precautionary step




Sigh

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OfflineProbationBlows
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Registered: 03/19/11
Posts: 51
Last seen: 12 years, 6 months
Re: Karo and sterilization [Re: ozzysmygod]
    #14613468 - 06/14/11 05:36 PM (12 years, 8 months ago)

ok sweet thank you guys...  madhatter dont be a dick please :smile: im just trying to learn not get fucking degrading lecture lol

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