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InvisibleSclerite
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Registered: 07/15/01
Posts: 14
A little data
    #1460228 - 04/15/03 02:16 PM (14 years, 8 months ago)

Thus far, in a an unknown space and time, the following has been recorded:

PES Amazonia
Huatla

substrates (all organic except shitake):
2) Quinoa
2) Black Quinoa
2) Millet
2) Amaranth
2) Rye seed
1) Texturized vegetable protein (TVP) (PES Amazonia)
1) Dried and crumbled shitake mushrooms (Huatla)
1) Soy Flour and vermiculite (50/50) (PES Amazonia)
1) Bat Guano and vermiculite (50/50) (Huatla)

Procedure:

Grains soaked over simmering distilled water for 1 hour

Grains sterilized by pressure (12.5 psi) for 1 hour in 1 pint wide mough mason jars fitted with coffee filter cut out under lids

Spore prints (1 ea.) made into sterile syringes and inoculated into jars, 5 cc each jar

All remaining unused substrates combined with addition of soaked birdseed and sterilzed as above in 1 quart mason jars.

Remaining spore solution incoculated into 1 quart jars (10 cc each), totalling five jars.

14 days post inoculation:

5 jars completely colonized by mycelium

2 - PES amazonia - Millet, Rye
3- Huatla - Millet, Rye, Shitake mushroom

Millet colonized both strains soonest, took longer for rhizomorphic growht

Rye shows strongest rhizomorphic growth

Shitake mushroom slow to begin colonizing, rapdily overtook other substrates in colonization time, less rhizomorphic growth - upon casing, decidely wetter, indicating better mositure retention than other substrates

Notes:
Amaranth very dense and mycelium penetrating slowly, although good growth

All Quinoas nearly colonized

Soy Flour dried and hardened - dead substrate
Bat guano (strong ammonia smell at onset) - dead substrate
TVP - turned mushy in sterilization, minimal growth

Quart jars:

3 jars partly colonized, but slow
2 jars uncolonized (suspect bacterial contamination, but no smell or color). Rate of colonization of quart jars much slower than pint jars: suspect using more pint jars more expeditious (based on previosu trials with conventional substrates) than volume afforded by quart jars

5 pint jars cased with 1 inch casing in plastic containers using peat/dolomitic lime/perlite casing and adjusted for pH and mositure content by the addition of saturated limewater (minus precipitate).

Until the next report....


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Offlinecanid
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Re: A little data [Re: Sclerite]
    #1462830 - 04/16/03 03:40 AM (14 years, 7 months ago)

in what way are dried and crumbled shiitake fruitbodies inorganic?
or am i missreading your post?

the presence of an amonia odor in the bat poo indicates improper (or no) composting. this may be easily remedied and i would be interested in hearing results as i've not heard of it being tried here at the shroomery.

all in all, good post.


--------------------



Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


Edited by concretefeet (04/16/03 03:43 AM)


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InvisibleSclerite
Stranger
Registered: 07/15/01
Posts: 14
Re: A little data [Re: canid]
    #1463104 - 04/16/03 08:17 AM (14 years, 7 months ago)

All the grains/flours were produced/grown organically (without pesticides, etc.) - I have no idea if the dried shitakes were produced in such a manner as they are not labelled as such. The rationale for trying these was that mushroom compost is not a particularly good substrate as it is exhausted. However, fruit bodies are not, and other mushrooms have relatively high levels of trytophan - Being a dry substrate, able to hold a moisture content equivalent to other mushrooms and substrate requirements, and just generally being curious, it was somewhat surprising to see such results.


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