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OfflineProbationBlows
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Karo and sterilization
    #14602079 - 06/12/11 05:17 PM (12 years, 7 months ago)

Hey shroomery! i gotta quick question

so i made a karo solution last night using my microwave and inoculated it with 2cc of golden teacher. im wondering how to suck up the solution once the myc starts to form. idk how this can be done in a sterile environment? how to i sterilize my syringe so that i can suck up the solution? (btw i dont own a PC)


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OfflineDungeonMaster
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Re: Karo and sterilization [Re: ProbationBlows]
    #14602191 - 06/12/11 05:36 PM (12 years, 7 months ago)



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OfflineProbationBlows
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Re: Karo and sterilization [Re: DungeonMaster]
    #14606146 - 06/13/11 01:05 PM (12 years, 7 months ago)

ok but how to i sterilize my syringe so that i can suck up a solution... i know how to make a syringe from a print but i need to know how to sterilize it so that i can suck up my karo solution.


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Offlinejust me
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Re: Karo and sterilization [Re: ProbationBlows]
    #14606182 - 06/13/11 01:12 PM (12 years, 7 months ago)

w/out a PC it might be hard to STERILIZE

but you can clean them up pretty close to sterile :lol: with alcohol, or hydrogen peroxide

washing either or both of those thru the syringe just like you would inject any liquid, cleans them well, but does not make them sterile


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OfflineMad_Hatter2004
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Re: Karo and sterilization [Re: ProbationBlows]
    #14606192 - 06/13/11 01:15 PM (12 years, 7 months ago)

Boil a pot of water and once at a rapid boil aspirate boiling water into the syringe and then purge into the sink.Repeat a few times,after purging the syringe for the last time just put the cap back on the needle and let cool.


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7 days without waves makes one weak!


Edited by Mad_Hatter2004 (06/13/11 01:17 PM)


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OfflineProbationBlows
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Re: Karo and sterilization [Re: just me]
    #14606193 - 06/13/11 01:16 PM (12 years, 7 months ago)

ok so just suck up some H2O2 and shake it around in my syringe then squirt it back out and its clean enough to knock up some jars?


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Offlinejust me
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Re: Karo and sterilization [Re: ProbationBlows]
    #14606319 - 06/13/11 01:39 PM (12 years, 7 months ago)

no scratch that
i forgot about boiling water

i would do a combination of all three or at least the boiling water with alcohol flush first


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-pEaCeLoVeGoDbLeSs-

"The Downfall of Mankind; is Believing He Has Limitations."


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Offlineozzysmygod
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Re: Karo and sterilization [Re: just me]
    #14606661 - 06/13/11 02:34 PM (12 years, 7 months ago)

Quote:

just me said:you can clean them up pretty close to sterile :lol: with alcohol, or hydrogen peroxide




This isnt true. They only destroy bacteria.


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Offlinejust me
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Re: Karo and sterilization [Re: ozzysmygod]
    #14606816 - 06/13/11 03:01 PM (12 years, 7 months ago)

whats not true


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"The Downfall of Mankind; is Believing He Has Limitations."


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Invisibleuncle bobo
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Re: Karo and sterilization [Re: just me]
    #14606830 - 06/13/11 03:03 PM (12 years, 7 months ago)

i always sterilize my syringes with boiling water, and have never had any problems with it.


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AKA Wolfgang Grajonza                 
IKO MYCO. MEDICINAL FARMS.


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Offlineozzysmygod
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Re: Karo and sterilization [Re: just me]
    #14607078 - 06/13/11 03:46 PM (12 years, 7 months ago)

Quote:

just me said:
whats not true




The statement you made that I quoted in the box


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OfflineMad_Hatter2004
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Re: Karo and sterilization [Re: ozzysmygod]
    #14607211 - 06/13/11 04:11 PM (12 years, 7 months ago)

Materials needed:

Empty syringes
One bottle of 70% isopropyl alcohol
Paper towels
A lighter or alcohol flame

Procedure One: Making a sterile syringe

1. Fill your saucepan halfway with tap or distilled water (use distilled water if your tap water contains higher levels of minerals and chemicals).
2. Boil the water in the saucepan on high for a minimum of ten minutes, this should be adequate to sterilize and cleanse the water of all bacteria and viruses.
3. Take your empty syringe and fill it with the boiling water. Allow it to sit for two minutes with the hot water inside.
4. Purge the hot water from the syringe into a sink, not back into the saucepan.
5. Repeat steps 3 and 4 two more times.
6. Place the capped syringe in a cool draft-free place, preferably in a clean zip-lock bag
7. Allow it to cool for several hours before proceeding to suck up/aspirate some LC from the jar.


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7 days without waves makes one weak!


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OfflineMOPE
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Re: Karo and sterilization [Re: Mad_Hatter2004]
    #14607550 - 06/13/11 05:21 PM (12 years, 7 months ago)

Like a few others have said

Before I got a PC I would aspirate boiling water 4-5 times, that worked for me

But im sure using iso and h202 couldnt hurt


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OfflineProbationBlows
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Re: Karo and sterilization [Re: MOPE]
    #14609215 - 06/13/11 10:09 PM (12 years, 7 months ago)

ok so when i go to suck up my karo solution i just purge the water in my syringe into the sink and proceed to suck up my solution? thats what im confused about... i dont know what to do once my syringe has water in it... i know how to make a syringe from prints  but im wonder from an lc


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OfflineMad_Hatter2004
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Re: Karo and sterilization [Re: ProbationBlows]
    #14610966 - 06/14/11 09:19 AM (12 years, 7 months ago)

Do you know how to read english man...for crying out loud.

Take an empty syringe.If it is an unopened never before used syringe then all you have to do is flame the tip of the needle and wipe with a clean 70% isopropyl alcohol soaked paper towel or napkin.

If it is a used syringe then you'll want to bring a pot of water to a full rolling boil.Take syringe and aspirate/suck up some of the boiling water until the entire syringe is filled with boiling water,walk to sink and purge/shoot the boiling water out of the syringe.Walk back to boiling water and aspirate/suck up more boiling water until the syringe is full yet again,walk back to sink again and purge the syringe emptying it of boiling water.Walk back to boiling water and aspirate yet again,back to sink and purge yet again.Back to boiling water,aspirate,back to sink and purge.Let empty syringe cool.

Wipe injection port on the LC lid with a clean 70% isopropyl alcohol soaked paper towel or napkin,flame sterilize tip of syringe needle,wipe with clean 70% isopropyl alcohol and push the needle through the injection port on the LC lid.Aspirate some LC into the syringe...boom you're ready to go.


--------------------
7 days without waves makes one weak!


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Offlineozzysmygod
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Re: Karo and sterilization [Re: Mad_Hatter2004]
    #14610980 - 06/14/11 09:25 AM (12 years, 7 months ago)

It is not recommended that you wipe a needle after flame sterilization with alcohol. Alcohol isnt sterile.......... so if you wipe a needle with alcohol after flaming it ,you could introduce contaminates.


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InvisibleFungal growth
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Re: Karo and sterilization [Re: Mad_Hatter2004]
    #14610985 - 06/14/11 09:26 AM (12 years, 7 months ago)

if you need it to suck up an lc just don't leave the
water in the syringe when you done. squirt it out and cap it.
then once its cool (not long with an empty syringe) use it
to suck up the lc.
copacetic?


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OfflineMad_Hatter2004
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Re: Karo and sterilization [Re: ozzysmygod]
    #14610992 - 06/14/11 09:28 AM (12 years, 7 months ago)

Never heard that one before,not in all my 7 years in the game.

Hell find me a tek that says not to use an alcohol soaked paper towel or napkin.


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7 days without waves makes one weak!


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Offlineozzysmygod
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Re: Karo and sterilization [Re: Mad_Hatter2004]
    #14611441 - 06/14/11 11:05 AM (12 years, 7 months ago)

Here is just one-

Quote:

RogerRabbit said:
If you flame sterilize, anything you do afterward will only make the needle 'dirtier'.  Why not just flame and use?  Forget the alcohol after flaming.  There is no need to cool down the needle.  Just use hot and let the first drop or two of solution cool the needle, so the rest can flow contaminant free.
RR




I have seen hundreds of posts in hundreds of threads from trusted cultivators  stating that wiping anything down with alcohol after flame sterilization only serves to make them more dirty. Ive seen a thread where RR says he's been trying for years to tell people to stop doing it, but people carry on anyway.

Alcohol is a sanitizer not a sterilizer. I might even make a spore syringe using 70% isopropyl alcohol to prove its ineffectivness at killing spores.

From wikipedia "As an antiseptic it is good against vegetative bacteria and fair against fungi and viruses, but is ineffective against spores."

...


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Invisibleuncle bobo
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Re: Karo and sterilization [Re: ozzysmygod]
    #14611466 - 06/14/11 11:10 AM (12 years, 7 months ago)

true that, but it does wash or help wipe spores away from the targeted area, like the silicone your putting the needle through.


--------------------

AKA Wolfgang Grajonza                 
IKO MYCO. MEDICINAL FARMS.


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OfflineMad_Hatter2004
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Re: Karo and sterilization [Re: ozzysmygod]
    #14611497 - 06/14/11 11:17 AM (12 years, 7 months ago)

Quote:

ozzysmygod said:
Here is just one-

Quote:

RogerRabbit said:
If you flame sterilize, anything you do afterward will only make the needle 'dirtier'.  Why not just flame and use?  Forget the alcohol after flaming.  There is no need to cool down the needle.  Just use hot and let the first drop or two of solution cool the needle, so the rest can flow contaminant free.
RR




I have seen hundreds of posts in hundreds of threads from trusted cultivators  stating that wiping anything down with alcohol after flame sterilization only serves to make them more dirty. Ive seen a thread where RR says he's been trying for years to tell people to stop doing it, but people carry on anyway.

Alcohol is a sanitizer not a sterilizer. I might even make a spore syringe using 70% isopropyl alcohol to prove its ineffectivness at killing spores.

From wikipedia "As an antiseptic it is good against vegetative bacteria and fair against fungi and viruses, but is ineffective against spores."

...





Not in any way shape or form trying to discredit RR (he's the man) but over the years I have seen him say one thing only to turn around and say the complete opposite at another time.

I'll continue to flame sterilize and then wipe with a clean paper towel soaked in isopropyl alcohol.Also,if you're going to quote someone,you might want to add what date that quote is taken from.


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Offlineozzysmygod
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Re: Karo and sterilization [Re: Mad_Hatter2004]
    #14611534 - 06/14/11 11:26 AM (12 years, 7 months ago)

The quote was taken from a thread in 07 but I've seen RR telling people not to wipe down syringes with alcohol this week. That was just one of the first search results.

He does sometimes update his methods, where research and experimentation proves old methods ineffective. But no matter how much research and experimentation you carry out alcohol, isopropyl or otherwise, isnt just going to miraculously start destroying spores.

Wikipedia says in black and white that rubbing alcohol does not kill spores, which you have ignored in your last post.

I will form a thread when I have time listing the hundreds of quotes and sources stating that wiping needles down with alcohol is ineffective.


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Offlinetotal
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Re: Karo and sterilization [Re: ozzysmygod]
    #14611593 - 06/14/11 11:38 AM (12 years, 7 months ago)

You guys need to find out whats right for you...
I have done both, both wipe and not...
They both work... :super:

BUT...
Now i just red hot a needle and shove it in there...
The logic is that, if its red hot, everything is dead and there is no need for the alcohol(other than cooling off the syringe so you dont waste a small amount of spores/lc to cool it)


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OfflineMad_Hatter2004
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Re: Karo and sterilization [Re: ozzysmygod]
    #14611605 - 06/14/11 11:41 AM (12 years, 7 months ago)

I never said it would kill contaminat mold spores,but it is an effective extra precautionary step IMHO.Hell it's even still shown on the lets grow mushrooms page.


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Offlineozzysmygod
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Re: Karo and sterilization [Re: Mad_Hatter2004]
    #14611660 - 06/14/11 11:53 AM (12 years, 7 months ago)

Quote:

ozzysmygod said:
From wikipedia "is ineffective against spores."





Quote:

Mad_Hatter2004 said:it is an effective extra precautionary step




Sigh


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OfflineProbationBlows
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Re: Karo and sterilization [Re: ozzysmygod]
    #14613468 - 06/14/11 05:36 PM (12 years, 7 months ago)

ok sweet thank you guys...  madhatter dont be a dick please :smile: im just trying to learn not get fucking degrading lecture lol


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