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OfflineJilPil
I'm a teapot


Registered: 06/07/11
Posts: 859
Last seen: 6 years, 8 months
Making more syringe filling from what I have...
    #14597811 - 06/11/11 08:35 PM (12 years, 8 months ago)


so i have all this stuff
distilled water inside the glass bottles with rubber stoppers on tops
a syringe made the same way as RR's "lets grow mushrooms" tek (purchased, looks really clear with some dark specs floating around)
should i add something to the distilled water so the myc can really make the water noticeably different looking (like they made the water thicker by colonizing or reproducing?)

also am i going about this wrong? please stop me or give a quick tip

thanks
~Jil

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OfflineMOPE
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Re: Making more syringe filling from what I have... [Re: JilPil]
    #14597878 - 06/11/11 08:48 PM (12 years, 8 months ago)

Sorry, your question is pretty confusing, are you asking how to make a liquid culture? 

Or are you asking how to make the spores spread around in the water more?

Spores dont just "reproduce", or for that matter germinate in plain old water.

Replacing the distilled water with spring water should help prevent spores from clumping so much


--------------------
Got used to the feeling of falling
But you'll never see me following
Bouncing back and forth between the healing and the hollering
Riding the outer ring of my own private saturn
Thoughts scattered all across the grey matter

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OfflineWing
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Re: Making more syringe filling from what I have... [Re: MOPE]
    #14597910 - 06/11/11 08:57 PM (12 years, 8 months ago)

I think he wants to increase his spore solution by diluting it.

They look too small for LC but I like a big jar to get some swishing action in and make sure there is enough oxygen to go around if I need to leave it for a while


--------------------
My Old Grow Logs


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InvisibleCarl Sagan
Time Dilation Analyst


Registered: 04/19/11
Posts: 922
Loc: Myco-tek.org
Re: Making more syringe filling from what I have... [Re: Wing]
    #14598168 - 06/11/11 10:02 PM (12 years, 8 months ago)

Whatever you are going to do PC the jars, a syringe body filled with poly fill, and the water first.  Then use your syringe poly filter to equalize pressure before sticking your spore
syringe in.:thumbup:


--------------------
“Sacred cows make the best hamburger”

Mark Twain



Independant Research Foundation

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OfflineCheeseBurgler
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Re: Making more syringe filling from what I have... [Re: Carl Sagan]
    #14598539 - 06/11/11 11:26 PM (12 years, 8 months ago)

i think the OP is referring to liquid culture, i think.

in that case, Damion 5050's Elementary Liquid Culture Tek  boom! do this.

actually, you should do whatever you want, but DO NOT just spread around your MS syringes into other jars. unfortunately, it wont grow. its not quite THAT easy, but it IS easy to make LC! trust me (and the tek) (trust the tek before me though):tongue2:


but, if thats not your style...
colonize a cake, suck some (pc-ed) spring water into a (sterilized) syringe, shoot it between the glass and the cake, shake for a moment, then suck it back out. TADA! a new MS syringe! it works, but you HAVE TO BE STERILE, and use your brain. 

feel free to pm.


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OfflineJilPil
I'm a teapot


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Re: Making more syringe filling from what I have... [Re: CheeseBurgler]
    #14598771 - 06/12/11 12:13 AM (12 years, 8 months ago)

after the first post i learned what i needed to search up
i just didnt know the name
but everything i just read from all of you was awesome
thanks :smile:
:thumbup:

this thread can be closed now if a mod wants to

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OfflineCheeseBurgler
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Re: Making more syringe filling from what I have... [Re: JilPil]
    #14598804 - 06/12/11 12:21 AM (12 years, 8 months ago)

best of luck making this happen! we're all rooting (myc-ing?) for you! remember to ask question


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OfflineJilPil
I'm a teapot


Registered: 06/07/11
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Re: Making more syringe filling from what I have... [Re: CheeseBurgler]
    #14599006 - 06/12/11 01:06 AM (12 years, 8 months ago)

wasn't trying to disregard i just really like these little vials :3

ok so i did a test pc with the jars with the lids and they popped off
so i "figured"...
put tight foil around the lid spot of each one
and separately in a strainer did the lids
swapped the water out in the little vials added karo
pc for 30 and let it cool a tad
quickly moved all the jars from the pc to the box with holes that i cleaned with bleach wipes
rubbed alcohol on my hands
quickly put the lids on

was the fact that i didn't have a tight lid on them going to be my downfall?

how long should i wait before putting my pan cyans in them?
how long before i know there are no bact

here is the pic timeline

Edited by JilPil (06/12/11 01:11 AM)

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OfflineMad_Hatter2004
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Re: Making more syringe filling from what I have... [Re: JilPil]
    #14599159 - 06/12/11 01:44 AM (12 years, 8 months ago)

Are you trying to make more spore solution,or a Liquid Culture?

I'd just make a Liquid Culture using some water and honey or karo.

Simply mix up the proper amounts of water and honey or water and karo and then place in a jar with a tight fitting lid,injection port,and some sort of GE filter like tyvek.Throw in the PC for 20 minutes and let cool for a few hours or overnight.Once cooled,inoculate the karo or honey water with 1-2cc's of spore solution from your 10cc syringe.Let the LC sit aside for a few days and then inspect for mycelium growth.Once you have sufficient enough growth you can swirl the jar around to break the mycelium cloud up into smaller pieces (it helps to have a marble or glass shards in the LC) and then suck some up into a blank sterile syringe.Store the leftover LC in the fridge for later use.Knock up a few test jars with the LC and wait to see signs of colonization and also to make sure there is no bacterial contamination.If all is good,start knocking up your jars and they will colonize 2-3x faster then if you inoculated with spores.This also saves you 8-9cc's of spore solution to make more LC with,which in turn will make you an efficient cultivator as one syringe can last a long long long time.

http://www.shroomery.org/11341/Liquid-Culture

http://www.shroomery.org/9393/FastFreds-Media-Cookbook


--------------------
7 days without waves makes one weak!

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OfflineJilPil
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Re: Making more syringe filling from what I have... [Re: Mad_Hatter2004]
    #14599343 - 06/12/11 03:08 AM (12 years, 8 months ago)

liquid culture because im not inoculating anything too soon

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OfflineMad_Hatter2004
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Re: Making more syringe filling from what I have... [Re: JilPil]
    #14599508 - 06/12/11 05:09 AM (12 years, 8 months ago)

You didn't use just straight karo did you..your supposed to use 4ml/cc's of karo to 96ml/cc's water...96% water and 4% karo.

I don't know how sterile those little LC's will be since all they have is a foil lid.

You should by some 1/2 pint canning jars with bands/lids and then get yourself some rtv silicone to make self healing injection ports int he lids,then use tyvek,or syringe filters as GE filters for the lids.

With that said I probably wouldn't inoculate those with your Panaeolus Cyanescens spores.I'd make a proper LC jar with self healing injection port and tyvek or syringe filter as the GE filter.

Typically you won't know if an LC is good or not just by looking at it,although sometimes it's plainly obvious that it is contaminated.The best thing you can do is knock up a few test jars with some of the LC and then wait for signs of colonization.If all is well,then you can proceed to make up all the jars you want,sterilize them and let them cool,and the inoculate with and LC syringe.


--------------------
7 days without waves makes one weak!

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Offlinelastboss
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Re: Making more syringe filling from what I have... [Re: Mad_Hatter2004]
    #14599570 - 06/12/11 05:48 AM (12 years, 8 months ago)

If you're not inoculating anything too soon it is really best kept in the syringe, lasts longer, and if you bought it from a good vendor you know it's clean and shouldn't cause contams. You never really know with LC's until you test them.

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OfflineCheeseBurgler
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Re: Making more syringe filling from what I have... [Re: lastboss]
    #14600473 - 06/12/11 11:56 AM (12 years, 8 months ago)

not to be a dick, but firstly, putting a bungie cord on a the lid of a pot doesn't make it a pressure cooker. actually, it makes it a pot, with a lid, and a bungie cord.

secondly, when making liquid cultures, they are going to have to breath, so thats why you put a noc port and a filter in the lid. your tops pop off because they are wrong, and because there is no air exchange as pressures change.

once something is pressure cooked (for real), you let it cool over night. hot ass fucking 'just boiled' water is gonna kill anything you put into it. makes sense huh.

also, one pressure cooked, they stay sterile for a while (like as long as you dont sneeze on them, or take the foil off, or get the filter wet), so there is no reason to rush them off to your Still Air box.

GREAT JOB having a still air box though!!! kudos for that!!

and a couple closing notes...wtf is the third piture? i mean, i get it you smoked a bowl (maybe?) but how outta that? i dont get it. did you boil that too?

and what would REALLY REALLY REALLY help is a little grammar checking once you write your message. i understand if english is not your first language, cuz thats cool, but maybe try Google translate or something next time. basically, its hard to understand you.


oh, and, um, again not to be a dick, but did you even look at the tek i sent you? or what Mad Hatter had to say? its gold, thats why we spent the time to send it.

never the less, you'll get it right...just keep trying...but read the damn tek! and follow the directions exactly, dont make up your own shit!

luck.


--------------------

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OfflineMOPE
Walking Chemical Reaction


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Re: Making more syringe filling from what I have... [Re: CheeseBurgler]
    #14600577 - 06/12/11 12:19 PM (12 years, 8 months ago)

Quote:

YouKnowEdDavis said:
not to be a dick, but firstly, putting a bungie cord on a the lid of a pot doesn't make it a pressure cooker. actually, it makes it a pot, with a lid, and a bungie cord.




:laugh2: QFT I was gonna say this too


--------------------
Got used to the feeling of falling
But you'll never see me following
Bouncing back and forth between the healing and the hollering
Riding the outer ring of my own private saturn
Thoughts scattered all across the grey matter

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OfflineJilPil
I'm a teapot


Registered: 06/07/11
Posts: 859
Last seen: 6 years, 8 months
Re: Making more syringe filling from what I have... [Re: CheeseBurgler]
    #14600800 - 06/12/11 01:04 PM (12 years, 8 months ago)

i hate that pot when the lit just sits there and rattles i was holding it down with the cord...

nothing has gone wrong so far
i still have my syringe of pan c

i looked at them forever, also read all the comments in those threads too... i just took your advice from before and did whatever i wanted.

im going to wait a while before i consider putting anything into the little vials. i wanna see if they are even contaminant free (actually what is the best amount of time before i know its good?)

i just started mycology i having fun experimenting :smile:

i just made 5 random sized jars of flax and rye flower that nobody eats at my house
for no reason but at least ill know the outcome of those substrates if someone asks about them

that's why im trying to make more LC for the future

i mean when i go to do my bulk ill be doing it verbatim of a really nice tek.



its a gravity bong i made. (pg. 2 has a video)
http://forum.grasscity.com/toking-tools/800640-glass-glass-grav-bong.html

edit:"not to be a dick" just makes the person think your going to say something dickish, so they expect it.

i read your whole post without reading "not to be a dick" and you didn't sound like a dick to me it was helpful
but as soon as you said that it made me upset lol.

Edited by JilPil (06/12/11 01:11 PM)

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OfflineCheeseBurgler
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Re: Making more syringe filling from what I have... [Re: JilPil]
    #14600992 - 06/12/11 01:42 PM (12 years, 8 months ago)

nothing will grow in the vials if they are contamed. they will just sit there, and look like water forever, unfortunately.

what exactly was in the vials to begin with? spring water? did you buy them like that? im not sure they would be practical for anything...

i understand the bungie cord now. makes sense, it just looked a little funny at first.

and OHHHHH....its a Grav Bong! i get it! too cool! i watched the video, sweet dreds; i like that you called it milk (the smoke, not your hair).

anywho, thanks for explaining what was going on, i know I appreciate it......and ill never be a "not dick" to you again, my bad.  :foshizzle:


hit us up when you get to doin some other stuff...we'll be here.


--------------------

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OfflineJilPil
I'm a teapot


Registered: 06/07/11
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Re: Making more syringe filling from what I have... [Re: CheeseBurgler]
    #14601139 - 06/12/11 02:08 PM (12 years, 8 months ago)

Quote:

YouKnowEdDavis said:
nothing will grow in the vials if they are contamed. they will just sit there, and look like water forever, unfortunately.

what exactly was in the vials to begin with? spring water? did you buy them like that? im not sure they would be practical for anything...

i understand the bungie cord now. makes sense, it just looked a little funny at first.

and OHHHHH....its a Grav Bong! i get it! too cool! i watched the video, sweet dreds; i like that you called it milk (the smoke, not your hair).

anywho, thanks for explaining what was going on, i know I appreciate it......and ill never be a "not dick" to you again, my bad.  :foshizzle:


hit us up when you get to doin some other stuff...we'll be here.




well i did have distilled water in them but replaced it with regular water
i didnt have some spring around the house so i used the Britta filter

i figured instead of using the cyans. id do that squirt water in then suck out thing with a jar i have that's colonized that way i wont waste the cyans if/when they come up contaminated

i got the little vials a long time ago and the rubber plugs too
they have ml markings on them :wink:..... that's about it lol

yeah ill probably get  more regular jars later on


hey if a jar is made pf tek way but never inoculated how long till its not able to be inoculated anymore?
i have some leftover jars from my first inoculation and after learning that colonized jar, syringe method id like to fill the rest of the jars up that i didnt have enough juice for.

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OfflineCheeseBurgler
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Re: Making more syringe filling from what I have... [Re: JilPil]
    #14601242 - 06/12/11 02:28 PM (12 years, 8 months ago)

the old "squirt it in, suck it out" method is THE SHIT!!! never will you be without a MS syringe! there is nothing wrong with this tek, and it works every time. just BE STERILE and use your brain!

when you replace water, they have to be re-sterilized. i know it sucks, but thats the way it goes. 

hmm....the timeline on how long a pf jar stays ready to be inoculated? umm.....as long as it has been stored with care, probably a long as time; maybe 3 months? i dont know from experience, but i mean, its just flour and verm and water in there...soooooooo probably way longer than 3 months, im just trying to give you a safe answer.

and hell yeah, if your other jars are ready, STERILIZE YOUR SYRInGEs, USE (sterile) SPRING WATER, and do the "squirt 'n' suck," you'll be amazed. shit, i used my own MS for nocing up grains, no problems, no contams, end of story.

maybe the britta filter will work, but i KNOW that spring water is the best for me.

pressure cookers are 35 dollars at walmart. not only can you sterilize EVERYTHING, but you can make food in it too.....mmmmmmmmmmm, foooooooood.....


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OfflineJilPil
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Re: Making more syringe filling from what I have... [Re: CheeseBurgler]
    #14601822 - 06/12/11 04:15 PM (12 years, 8 months ago)

do the jars have to be like 110% colonized to do the squirt and suck?

its funny i feel good when i know i messed up

at least i got a good question out of it

im going to start writing up checklists to follow while in the heat of mycology so i wont make as many stupid little errors

might go to walmart right now... i thought they were like 100  at any store and online for like 300  for big ones
also going to post office lol i gotta get some tyvek

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OfflineCheeseBurgler
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Re: Making more syringe filling from what I have... [Re: JilPil]
    #14602288 - 06/12/11 06:00 PM (12 years, 8 months ago)

uh, 100% colonized will do fine. you could probably even do it before the 1 week consolidation period. but i wouldn't do it any sooner than that; risk suckin up some verm into the syringe and it getting stuck, and cuz it just makes sense.

checklist, YES!!! do it! that is a GREAT idea! i always take notes when i'm reading all this info.

post office, YES!!! tyvek is the way to go! oh, and bandaids. get the "tough strips WATERPROOF" kind, top left -->http://www.bandaid.com/our-products/durable-protection

(cut a little square of tyvek just big enough to cover your GE hole, then cut the sticky part off the bandaid and stick it down. THOSE kind of bandaids survive the dishwasher and the PC, and they let air exchange with exchanging water.)

walmart PC, YES!!! its by a company  called "wearever" and i think it comes in an 8 quart and a 6 quart. i just got the biggest one, cuz why not? 35$ is what i paid for my "biggest one," so look for that.

might want to pick up some jars while your there....1/2 pint, wide mouth.

hell yeah!!! im excited for you!


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