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 poke smot!
floccinocci floofinator
Registered: 01/08/03
Posts: 5,251
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 Agar overflowing 
#1459832 - 04/15/03 09:45 AM (21 years, 7 months ago) |
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Hello... I'm sure I have read this somewhere but now that I need the information I can't find it anywhere. I'm pouring agar plates, but the past few times I've found that the agar quickly finds itself on the inside of the PC.
I fill pint jars (thats all that fits in this PC till the new one arrives) with the mix, about 125ml's of solution (which is less than half full). Then I cover with tinfoil and PC for 45 minutes. But even so, I keep finding the agar bubbling out of the containers and all that I am left with is less than 100ml's of agar, and gooey shit coating the PC 
Any ideas?
Edited by poke smot! (09/07/20 01:23 PM)
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 stefan
work in progress
Registered: 04/11/01
Posts: 8,932
Loc: The Netherlands
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Re: Agar overflowing [Re: poke smot!]
#1459886 - 04/15/03 10:15 AM (21 years, 7 months ago) |
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is it really nescissairy to sterilize agar in a pc? can't you just put it in your microwave? that way you can easier control the 'overflowing' of the agar...
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Unknown
Confused,dizzy,wheream I again?
Registered: 01/06/02
Posts: 380
Loc: MA
Last seen: 20 years, 8 months
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Re: Agar overflowing [Re: stefan]
#1460269 - 04/15/03 12:29 PM (21 years, 7 months ago) |
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Hmm,I wonder if it is possible in a microwave.
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The above is just like,my opinion man
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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 Re: Agar overflowing [Re: poke smot!]
#1460299 - 04/15/03 12:40 PM (21 years, 7 months ago) |
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I used to use Quart jars with a tyvek filter disc and the lid almost tight.
Worked like a charm.
I'm sure if you did the same with a Pint jar you would be fine.
good luck!
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
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deanofmean
mycophagous
Registered: 12/06/02
Posts: 2,017
Loc: PNW
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Re: Agar overflowing [Re: stefan]
#1460989 - 04/15/03 04:06 PM (21 years, 7 months ago) |
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yes, you can sterilize agar in a microwave .
do small batches, and add a little extra water .
nuke 3-4 min. at a time, and watch out for boil over .
i have a dinky 700 watt m/w so i nuke a half full pint jar for a total of 8 min.
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Unknown
Confused,dizzy,wheream I again?
Registered: 01/06/02
Posts: 380
Loc: MA
Last seen: 20 years, 8 months
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Re: Agar overflowing [Re: deanofmean]
#1461022 - 04/15/03 04:18 PM (21 years, 7 months ago) |
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Damn,that's dope as hell.
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The above is just like,my opinion man
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NoLogos
newbie
Registered: 02/28/03
Posts: 37
Loc: South US
Last seen: 20 years, 9 months
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Re: Agar overflowing [Re: Anonymous]
#1461330 - 04/15/03 05:53 PM (21 years, 7 months ago) |
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I would guess that your PC is cooling off too fast. That would cause vigorous boiling as the pressure drops, causing your boiling over problem.
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afoaf
CEO DBK?
Registered: 11/08/02
Posts: 32,665
Loc: Ripple's Heart
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Re: Agar overflowing [Re: NoLogos]
#1461661 - 04/15/03 07:44 PM (21 years, 7 months ago) |
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nice observation...seriously.
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All I know is The Growery is a place where losers who get banned here go.
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 canid
irregular meat sprocket
Registered: 02/26/02
Posts: 11,936
Loc: looking for zeebras, n. c...
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Re: Agar overflowing [Re: afoaf]
#1462820 - 04/16/03 01:31 AM (21 years, 7 months ago) |
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road, it also works to use those juice bottles with graduated necks (for ease of pouring) then puncturing the lid and stuffing with pollyfill.
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Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.
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zeronio
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Re: Agar overflowing [Re: Roadkill]
#1462828 - 04/16/03 01:39 AM (21 years, 7 months ago) |
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I do it exactly like Roadkill.
Tyvek filter is great also because it filters air as it enters the jar when it's cooling down.
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canid
irregular meat sprocket
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Re: Agar overflowing [Re: zeronio]
#1462851 - 04/16/03 02:00 AM (21 years, 7 months ago) |
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Quote:
Tyvek filter is great also because it filters air as it enters the jar when it's cooling down.
good point.
--------------------
Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,167
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Re: Agar overflowing [Re: poke smot!]
#1463057 - 04/16/03 05:25 AM (21 years, 7 months ago) |
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It happens if the pressure cooker cools down too fast. Make sure to let it naturally cool down slowly until the pressure is back to atmospheric. Only then open the PC and take the bottle(jar) out.
This is usually a problem with thicker agar formulations, for instance oatmeal agar or dogfood agar. Less common with PDA or MEA.
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poke smot!
floccinocci floofinator
Registered: 01/08/03
Posts: 5,251
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Re: Agar overflowing *DELETED* [Re: Anno]
#1463868 - 04/16/03 01:16 PM (21 years, 7 months ago) |
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Post deleted by poke smot!Reason for deletion: x
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Hafrican
Mega-ultrachicken
Registered: 01/31/03
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Loc: SW Azerbaijan
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Re: Agar overflowing [Re: poke smot!]
#1465389 - 04/16/03 09:16 PM (21 years, 7 months ago) |
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Nice PC 
On my All-American, the warning label is where your gauge is :P
Lol ... such a well-crafted item .... enjoy!
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woodrow
journeyman
Registered: 03/17/03
Posts: 142
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Re: Agar overflowing [Re: Hafrican]
#1466254 - 04/17/03 03:32 AM (21 years, 7 months ago) |
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Fifteen minutes of sterilizing in a PC is usually enough for agar.
Instead of pouring petri dishes, I prefer to use flat sided bottles...liqueur bottles or prescription bottles. Microwaves are great for preparing and melting agar but they do a poor job of sterilizing. I like to put about 10 or 15 ml of freshly prepared agar into flat sided bottles and sterilize for 15 min in a PC and then let the PC cool SLOWLY. When the PC has cooled enough to handle the contents, remove the bottles and lay them on their sides until the agar is nice and solid. The bottles can be stored for many months on a shelf. It is a bit of a problem to work with agar through the narrow neck of a bottle but it is not hard to fashion tools for that purpose from heavy wire or bicycle spokes.
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Killjoy
TheHyperdimensionalSlug
Registered: 01/28/03
Posts: 865
Last seen: 10 years, 3 months
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Re: Agar overflowing [Re: woodrow]
#1466519 - 04/17/03 07:44 AM (21 years, 7 months ago) |
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Put Agar in flask (erlenmyer), stuff cotton-ball in neck of flask, swirl, put in microwave.
Nuke the bitches for 15 minutes at power 30.
Pour onto plates.
Done.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Agar overflowing [Re: Killjoy]
#1480652 - 04/21/03 10:21 PM (21 years, 7 months ago) |
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Another thing that will make your agar overflow is if the heat is up too high during sterilization. Anytime steam is escaping the top of your pc, it is also escaping your agar. Once you reach 15lbs, turn the heat down as low as possible to maintain that pressure. Since the pressure inside the agar bottle is also at 15psi, anytime the little weight on the top of the PC rattles and lets off steam, the agar is allowed to boil in the bottle. This causes it to 'boil over' just like a pot of sauce on the stovetop. And of course, like was said earlier, if the pc cools down to fast, the pressure in the agar bottle is higher than the pressure in the PC, and it boils over.
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Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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I use a screw cap elrenmeyer, tyvek under the cap and the cap screwed about half way down...I never worry about boilover....I got about 18 of those....250ml to 1000ml...they work well....
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Odin 13
Excitable Boy
Registered: 01/07/00
Posts: 62
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Re: Agar overflowing [Re: Prisoner#1]
#1491419 - 04/24/03 10:41 PM (21 years, 7 months ago) |
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With that AA PC, if it has the little stopcock type of bleedoff valve (mine does) you merely need to unscrew it a little bit until it starts to bleed off steam at the end of your sterilisation cycle. It takes mine about 5 minutes to 7 minutes to bleed off from 15-17 lbs/". That slow rate will prevent boilover caused by rapid depressurization at cycle end.
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Anonymous
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Re: Agar overflowing [Re: Odin 13]
#1491469 - 04/24/03 11:02 PM (21 years, 7 months ago) |
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Try getting one of these:
Here's my AA25X Electric Steam Sterilizer Stereoclave:
More pictures here:
http://www.shroomery.org/forums/showflat.php?Cat=&Board=Growlog&Number=1447682&fpart=&PHPSESSID=
Meant for sterilizing, not like a PC, so may help you out.
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