Home | Community | Message Board


High Mountain Compost
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Petri Dish, Potato Dextrose, Pressure Cooker

Jump to first unread post. Pages: 1
Invisiblepoke smot!
floccinocci floofinator
Male

Registered: 01/08/03
Posts: 5,231
Agar overflowing *DELETED*
    #1459832 - 04/15/03 11:45 AM (13 years, 7 months ago)

Post deleted by poke smot!

Reason for deletion: x



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinestefan
work in progress

Registered: 04/11/01
Posts: 8,932
Loc: The Netherlands
Last seen: 11 days, 8 hours
Re: Agar overflowing [Re: poke smot!]
    #1459886 - 04/15/03 12:15 PM (13 years, 7 months ago)

is it really nescissairy to sterilize agar in a pc? can't you just put it in your microwave? that way you can easier control the 'overflowing' of the agar...


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineUnknown
Confused,dizzy,wheream I again?
Registered: 01/07/02
Posts: 380
Loc: MA
Last seen: 12 years, 8 months
Re: Agar overflowing [Re: stefan]
    #1460269 - 04/15/03 02:29 PM (13 years, 7 months ago)

Hmm,I wonder if it is possible in a microwave.


--------------------
The above is just like,my opinion man


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: Agar overflowing [Re: poke smot!]
    #1460299 - 04/15/03 02:40 PM (13 years, 7 months ago)

I used to use Quart jars with a tyvek filter disc and the lid almost tight.
Worked like a charm.

I'm sure if you did the same with a Pint jar you would be fine.

good luck!


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: Agar overflowing [Re: stefan]
    #1460989 - 04/15/03 06:06 PM (13 years, 7 months ago)

yes, you can sterilize agar in a microwave .
do small batches, and add a little extra water .
nuke 3-4 min. at a time, and watch out for boil over .
i have a dinky 700 watt m/w so i nuke a half full pint jar for a total of 8 min.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineUnknown
Confused,dizzy,wheream I again?
Registered: 01/07/02
Posts: 380
Loc: MA
Last seen: 12 years, 8 months
Re: Agar overflowing [Re: deanofmean]
    #1461022 - 04/15/03 06:18 PM (13 years, 7 months ago)

Damn,that's dope as hell.


--------------------
The above is just like,my opinion man


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineNoLogos
newbie
Registered: 02/28/03
Posts: 37
Loc: South US
Last seen: 12 years, 9 months
Re: Agar overflowing [Re: Anonymous]
    #1461330 - 04/15/03 07:53 PM (13 years, 7 months ago)

I would guess that your PC is cooling off too fast. That would cause vigorous boiling as the pressure drops, causing your boiling over problem.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleafoaf
CEO DBK?
 User Gallery

Registered: 11/08/02
Posts: 32,665
Loc: Ripple's Heart
Re: Agar overflowing [Re: NoLogos]
    #1461661 - 04/15/03 09:44 PM (13 years, 7 months ago)

nice observation...seriously.


--------------------
All I know is The Growery is a place where losers who get banned here go.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinecanid
irregular meat sprocket
Male User Gallery

Folding@home Statistics
Registered: 02/26/02
Posts: 11,792
Loc: looking for zeebras, n. c...
Last seen: 5 days, 23 hours
Re: Agar overflowing [Re: afoaf]
    #1462820 - 04/16/03 03:31 AM (13 years, 7 months ago)

road, it also works to use those juice bottles with graduated necks (for ease of pouring) then puncturing the lid and stuffing with pollyfill.


--------------------



Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinezeronio
Stranger
Male

Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 3 months, 3 days
Re: Agar overflowing [Re: Roadkill]
    #1462828 - 04/16/03 03:39 AM (13 years, 7 months ago)

I do it exactly like Roadkill.
Tyvek filter is great also because it filters air as it enters the jar when it's cooling down.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinecanid
irregular meat sprocket
Male User Gallery

Folding@home Statistics
Registered: 02/26/02
Posts: 11,792
Loc: looking for zeebras, n. c...
Last seen: 5 days, 23 hours
Re: Agar overflowing [Re: zeronio]
    #1462851 - 04/16/03 04:00 AM (13 years, 7 months ago)

Quote:


Tyvek filter is great also because it filters air as it enters the jar when it's cooling down.





good point.


--------------------



Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 17 days
Re: Agar overflowing [Re: poke smot!]
    #1463057 - 04/16/03 07:25 AM (13 years, 7 months ago)

It happens if the pressure cooker cools down too fast. Make sure to let it naturally cool down slowly until the pressure is back to atmospheric. Only then open the PC and take the bottle(jar) out.
This is usually a problem with thicker agar formulations, for instance oatmeal agar or dogfood agar. Less common with PDA or MEA.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblepoke smot!
floccinocci floofinator
Male

Registered: 01/08/03
Posts: 5,231
Re: Agar overflowing *DELETED* [Re: Anno]
    #1463868 - 04/16/03 03:16 PM (13 years, 7 months ago)

Post deleted by poke smot!

Reason for deletion: x



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHafrican
Mega-ultrachicken

Registered: 01/31/03
Posts: 105
Loc: SW Azerbaijan
Last seen: 9 years, 5 months
Re: Agar overflowing [Re: poke smot!]
    #1465389 - 04/16/03 11:16 PM (13 years, 7 months ago)

Nice PC :smile:
On my All-American, the warning label is where your gauge is :P
Lol ... such a well-crafted item .... enjoy!


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblewoodrow
journeyman
Registered: 03/17/03
Posts: 135
Re: Agar overflowing [Re: Hafrican]
    #1466254 - 04/17/03 05:32 AM (13 years, 7 months ago)

Fifteen minutes of sterilizing in a PC is usually enough for agar.
Instead of pouring petri dishes, I prefer to use flat sided bottles...liqueur bottles or prescription bottles. Microwaves are great for preparing and melting agar but they do a poor job of sterilizing. I like to put about 10 or 15 ml of freshly prepared agar into flat sided bottles and sterilize for 15 min in a PC and then let the PC cool SLOWLY. When the PC has cooled enough to handle the contents, remove the bottles and lay them on their sides until the agar is nice and solid. The bottles can be stored for many months on a shelf. It is a bit of a problem to work with agar through the narrow neck of a bottle but it is not hard to fashion tools for that purpose from heavy wire or bicycle spokes.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineKilljoy
TheHyperdimensionalSlug

Registered: 01/28/03
Posts: 865
Last seen: 2 years, 3 months
Re: Agar overflowing [Re: woodrow]
    #1466519 - 04/17/03 09:44 AM (13 years, 7 months ago)

Put Agar in flask (erlenmyer), stuff cotton-ball in neck of flask, swirl, put in microwave.

Nuke the bitches for 15 minutes at power 30.

Pour onto plates.

Done.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineRogerRabbitM
Bans for Pleasure
 User Gallery

Registered: 03/26/03
Posts: 41,968
Loc: USA Mountain Northwest
Last seen: 2 months, 15 days
Trusted Cultivator
Re: Agar overflowing [Re: Killjoy]
    #1480652 - 04/22/03 12:21 AM (13 years, 7 months ago)

Another thing that will make your agar overflow is if the heat is up too high during sterilization. Anytime steam is escaping the top of your pc, it is also escaping your agar. Once you reach 15lbs, turn the heat down as low as possible to maintain that pressure. Since the pressure inside the agar bottle is also at 15psi, anytime the little weight on the top of the PC rattles and lets off steam, the agar is allowed to boil in the bottle. This causes it to 'boil over' just like a pot of sauce on the stovetop. And of course, like was said earlier, if the pc cools down to fast, the pressure in the agar bottle is higher than the pressure in the PC, and it boils over.


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 191,700
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Agar overflowing [Re: RogerRabbit]
    #1485127 - 04/23/03 03:52 AM (13 years, 7 months ago)

I use a screw cap elrenmeyer, tyvek under the cap and the cap screwed about half way down...I never worry about boilover....I got about 18 of those....250ml to 1000ml...they work well....



Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleOdin 13
Excitable Boy

Registered: 01/08/00
Posts: 62
Re: Agar overflowing [Re: Prisoner#1]
    #1491419 - 04/25/03 12:41 AM (13 years, 7 months ago)

With that AA PC, if it has the little stopcock type of bleedoff valve (mine does) you merely need to unscrew it a little bit until it starts to bleed off steam at the end of your sterilisation cycle. It takes mine about 5 minutes to 7 minutes to bleed off from 15-17 lbs/". That slow rate will prevent boilover caused by rapid depressurization at cycle end.


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Agar overflowing [Re: Odin 13]
    #1491469 - 04/25/03 01:02 AM (13 years, 7 months ago)

Try getting one of these:
Here's my AA25X Electric Steam Sterilizer Stereoclave:


More pictures here:
http://www.shroomery.org/forums/showflat.php?Cat=&Board=Growlog&Number=1447682&fpart=&PHPSESSID=

Meant for sterilizing, not like a PC, so may help you out.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Petri Dish, Potato Dextrose, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Agar Overflow Jackal 793 5 12/09/02 06:53 AM
by DERRAYLD
* TYVEK AIR FILTER - Jars or air scrubber SixTango 2,292 2 01/28/02 12:42 PM
by SixTango
* Technical question about filter disks
( 1 2 all )
Anonymous 3,216 37 10/14/03 05:57 PM
by MrMaddHatter
* home made filter patch bags zeronio 5,014 16 05/18/02 07:09 AM
by goolag
* Oatmeal agar - 2 questions YSr 2,590 11 11/05/04 02:04 PM
by ATWAR
* IMPULSE SEAL TYVEK
( 1 2 all )
agar 2,788 27 01/06/05 01:22 PM
by PooPs
* Post deleted by MOE THE MAD SCIENTIST phreedom 1,843 8 12/27/02 08:28 AM
by cheesenoonions
* What Kind Of Lid To Use When Sterilizing Agar? glenbelt 1,079 8 02/17/09 05:33 PM
by fastfred

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
1,931 topic views. 0 members, 4 guests and 1 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Crestline Sales - MycoPath
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.059 seconds spending 0.003 seconds on 14 queries.