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OfflineAbraxis0
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14596563 - 06/11/11 03:22 PM (12 years, 8 months ago)

I dunno man, I'm not seeing trich. The first pic does look a tad funky but that could just be the lighting and angle. Trich is an emerald green color. So don't be too bummed about Nature Boys post (although he is dead right that it was a really poor decision to open a jar you thought was contaminated any where near your work area). In my experience Myco always loses to trich every time. Perhaps this thread should be moved to the Contam forum, mods?


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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Abraxis0]
    #14596570 - 06/11/11 03:24 PM (12 years, 8 months ago)

yea i ment to put it in contam forum it would be nice if a mod can move..

all i kno is it is definately a contam im not sure what tho.. could be yeast mold

i opened it in my garage.

pics aren't of best quality and lighting , but its a greyish-green colour in real life

Edited by explosivekush (06/11/11 03:29 PM)

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OfflineNature Boy
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14596589 - 06/11/11 03:31 PM (12 years, 8 months ago)

Quote:

explosivekush said:
damn

:feelsbadman:

so should i just toss all the jars and start in a month or so

its a wierd contam on the other jars, it-s greenish grey with small solid yellow things on  - around it





JEEEZZZZ!!!  Just get rid of them ALL!!!  PM me your address I'll send you the money for a new set of jars I PROMISE!!!!  Wash your clothes, take a shower, and knock your next set of jars up at a friends house!!  That's your only hope.

Edit:  Promise to throw them out, and I'll send you some spore syringes to boot!!!!!!!!!!!!!!

N.B.


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All submitted posts under this user name are works of pure fiction or outright lies.  Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit.  Note well:  Sorry, but I do not answer PM's unless you are a long-time trusted friend.  If you have a question, ask it in the appropriate thread.

                                                                               

Edited by Nature Boy (06/11/11 03:34 PM)

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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Nature Boy]
    #14596612 - 06/11/11 03:34 PM (12 years, 8 months ago)

thanks alot for the offer D: , keep your money though, ill buy me some new glass jars. 5 shrooms just for that offer though though i could use a syringe, im not sure if my vendor is good it's not a sponsor

what are your thoughts on my new jars. , toss them too?

-edit- the new jars might not be contam'ed it could be just a piece of discoloured verm , it would suck to toss out 2 batches in a row

Edited by explosivekush (06/11/11 03:40 PM)

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OfflineNature Boy
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14596635 - 06/11/11 03:43 PM (12 years, 8 months ago)

Well, you're the guy on the scene, and in the best position to judge.  Its a bit early, but if they are progressing along the same lines as the first batch, I wouldn't get your expectations too high.  Once it becomes apparent that there is contamination of any sort, you've GOT to get the jar O-U-T of your grow area ASAP.  Unfortunately, contam spores are VERY numerous, tiny, and "sticky"...like nettles.  They get EVERYWHERE and it takes scrupulous technique to succeed following a house-wide contamination, which you probably have, but don't realize.  I'm shocked no one else has come forward to acknowledge and second this fact.

Seriously, its OK to take me up on the offer.  Its genuine.  I have more mushies, syringes, and other supplies than I need or probably will ever use.  And jars don't cost tit, so that's no biggie, either.

I'd love to see you succeed, but it may to take more effort on your part given what has now happened.

N.B.


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All submitted posts under this user name are works of pure fiction or outright lies.  Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit.  Note well:  Sorry, but I do not answer PM's unless you are a long-time trusted friend.  If you have a question, ask it in the appropriate thread.

                                                                               

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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Nature Boy]
    #14596654 - 06/11/11 03:48 PM (12 years, 8 months ago)

ill for sure toss out those two old jars, far away from my house this time , and ill leave the new jars for another week before even looking at them. I sprayed the hell out of my room [workspace] and garage [ where i opened the jars ] with lysol,  trying to fight the contams. even though it will hardly do anything, it was worth a try :frown:.

edit- in a week ill post pics of the news jars

well thx ppl for all the help especially NB

Edited by explosivekush (06/11/11 03:50 PM)

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OfflineBreakfast Crew
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14596821 - 06/11/11 04:30 PM (12 years, 8 months ago)

Contamination from a dirty syringe would show around the inoculation zones. In this case the culprit was not properly sterilizing the jars.  Use a regular pot with a lid, add 1-2 inches of water and cover for 1 hour and 30 minutes. When it begins boiling start the timer and turn the heat low-medium.

You CANNOT save a contaminate jar. Toss it out and start over.

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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Breakfast Crew]
    #14596854 - 06/11/11 04:37 PM (12 years, 8 months ago)

i've heard of this method, but wouldn't a vegetable steamer be better, as there is hot steam from boiling water all over the jars

the first pics with almost the whole cake contam'ed was from the kit, the last pics were home-made and sterilized for 80 mins in a vegetable steamer kind of like this



its basically a pot with a section that you put in that has holes like a strainer. i put water in the bottom of the pot, then put the section with the holes on with the jars standing upright on it , and the lid over it. i started the count when it started boiling. 80 mins to be exact. i checked the water levels every 20 mins to make sure it doesn't boil dry.

Edited by explosivekush (06/11/11 04:40 PM)

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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Breakfast Crew]
    #14596858 - 06/11/11 04:37 PM (12 years, 8 months ago)

Quote:

Breakfast Crew said:
Contamination from a dirty syringe would show around the inoculation zones. In this case the culprit was not properly sterilizing the jars.

You CANNOT save a contaminate jar. Toss it out and start over.




Yep.

And go buy a pressure cooker at a thrift store for ten bucks. Just do it.


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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Doc_T]
    #14596863 - 06/11/11 04:39 PM (12 years, 8 months ago)

ok since your all pro's giving me this advice the next batch will be done with a glove box and pressure cooker

edit- nice boxy lol.

Edited by explosivekush (06/11/11 04:43 PM)

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Offline2jew4u
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14596878 - 06/11/11 04:41 PM (12 years, 8 months ago)

If you find a PC at a thrift store for ten bux- Buy all the ones you can!!!

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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14596884 - 06/11/11 04:42 PM (12 years, 8 months ago)

Gloves at least. Open air is smoother and easier than a glove box, so for shooting jars I don't recommend it usually.
Do wear a mask if you can and don't breathe on the needle.


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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Doc_T]
    #14596896 - 06/11/11 04:45 PM (12 years, 8 months ago)

the first batch, was done with gloves, alcohhol , flame sterilized needles , face masks and a room with all fans / vents / windows closed to minmize airflow and before inoculation the whole room was nuked with lysol disinfectant spray , so much that it hurt to breath without the mask.

the second batch was the same, just without the gloves because i only had the first two.


oh and before all this i even took a shower, and washed my hands-arms with anti-bacterial soap... but still all contams :frown:


just to know : is this contam trich, and the yellow spots yeast mold? this is what i was guessing

Edited by explosivekush (06/11/11 04:49 PM)

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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14596916 - 06/11/11 04:49 PM (12 years, 8 months ago)

So there you go- do it like that, but sterilize the jars better.
THEN if they contam, it's likely the syringe.

How can you tell? Leave one jar unshot.
If it stays good and the others turn green, it's the syringe.
If it turns green without being touched, your sterile prep is lacking.


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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Doc_T]
    #14596925 - 06/11/11 04:51 PM (12 years, 8 months ago)

i never thought of that , good advice ^^ i will definately do that next time

could it be that contams get in through the dry verm layer while the needle was puncturing it?

Edited by explosivekush (06/11/11 04:53 PM)

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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14596934 - 06/11/11 04:54 PM (12 years, 8 months ago)

Possible, unlikely.
It should be sterile under the foil- you pull the foil and shoot, then tape.
There's very little opportunity for contams to enter- and if they do, the growth will be under the holes, not throughout the jars.


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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Doc_T]
    #14596962 - 06/11/11 05:00 PM (12 years, 8 months ago)

ok. thanks for all the advice

i hope my current and future batches go good, ima toss those old jars faar away from my home :smile:


Edited by explosivekush (06/11/11 05:04 PM)

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OfflineAbraxis0
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14596980 - 06/11/11 05:06 PM (12 years, 8 months ago)

Quote:

explosivekush said:
i've heard of this method, but wouldn't a vegetable steamer be better, as there is hot steam from boiling water all over the jars

the first pics with almost the whole cake contam'ed was from the kit, the last pics were home-made and sterilized for 80 mins in a vegetable steamer kind of like this



its basically a pot with a section that you put in that has holes like a strainer. i put water in the bottom of the pot, then put the section with the holes on with the jars standing upright on it , and the lid over it. i started the count when it started boiling. 80 mins to be exact. i checked the water levels every 20 mins to make sure it doesn't boil dry.




That looks exactly like the one I've used over the past few years. I boil/steam my jars for almost 2 hours. Once boiling I'll add 4 cups of water every thirty minutes, I've never had a problem with it boiling dry. Contamination was rare.

I didn't use a glove box, and I shot the jars in open air. I did where gloves and a surgical mask, and I turned off the air conditioner 1 hour before shooting them up. Also used copius amounts of Alchohol & lysol to sterilize the entire area.


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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Abraxis0]
    #14597000 - 06/11/11 05:11 PM (12 years, 8 months ago)

it would be easier for me to use it rather then go out an buy a pressure cooker, but if i find one for cheap i will.

glad to know it works for you, i searched the forum before using it and some other people use it too

Edited by explosivekush (06/11/11 05:15 PM)

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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14597010 - 06/11/11 05:14 PM (12 years, 8 months ago)

For pf tek, steaming in a big pot with a heavy lid is ok.
But a pressure cooker is better, even a little one.
And you'll want to have a little one later anyway, no harm in buying it now.


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