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VaeVictum
Sativa Cyborg

Registered: 06/01/11
Posts: 4,397
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Concerned Pf Tek grower + wall of text = help please? <3
#14593214 - 06/10/11 10:20 PM (12 years, 7 months ago) |
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Hello, I am following "the original" pf tek of 1/2 cup verm 1/4 brf 1/4 cup water. I mixed all of my stuff well and got it very moist and distributed and lightly tamped it down leaving a 1/2 inch for dry verm.
My only concern so far on day 5 is if maybe my cakes dried out during the 60 minutes of steam sterilization. If I could of pressure canned I would of but circumstances happen.
I used filtered water and my vermiculite was sort of a mix of coarse and fine (you might be able to tell in the pic). The brown rice flour I made by grinding organic long grain brown rice that I bought with food stamps like a boss, it also ended up being coarse and fine.
I am fairly certain that during the inoculation I was pretty sterile but I understand you can never be too sterile. I sanitized my island in my kitchen with alcohol wipes and made sure the air was still as possible. I then washed my hands and even splashed some alcohol on my finger tips. I pulled out my syringe and had help re-taping the holes (they cleaned their hands the same way) so we did it incredibly fast.
I wiped down the syringe with alcohol wipes in between every jar and pulled my shirt over my face and looked silly as to not breath on the jars either.
I have no mycelium on day 5 and just am wondering if maybe my mixture was too dry or if I just need to be more patient. But I at least waited 5 whole days to make a thread!
Strains are listed in the pics and I have the tapered jar in one of the pictures as well.
My only questions for you guys are these three...
During inoculation I fucked up good and on my 5th jar I accidentally grabbed an unsterilized pf jar that I didn't have room to sanitize and inoculated it sterlized the needle with an alcohol wipe and did the jar I ment to do after that. I id'd the unsterilized jar and have it removed from the others.
1) Is there no hope for the accidental 6th jar and the 5th? 2) If my mycelium doesn't grow or has halted growth do I start over with left over spores? 3) There's too many opinions on a few aspects, band on tight band on loose is one and store them in indirect light or in a dark spot. (I know this makes it two questions but I labeled them as one.
Currently my 6 babies are in a small container on a book shelf low to ground out of direct sunlight it's an average of 65-70 in my aparment at all times.
Pictures are below and I apologize for the wall 'o text.


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Wing
The Eye Tyrant



Registered: 04/25/11
Posts: 3,293
Last seen: 5 months, 11 days
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Re: Concerned Pf Tek grower + wall of text = help please? <3 [Re: VaeVictum]
#14593281 - 06/10/11 10:34 PM (12 years, 7 months ago) |
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1- Just isolate the two jars. The one you didn't sterilize will contam most likely. The one you did after that may contam as well but maybe not. Thats just the way it is.
sometimes it takes a while to see germination. Start to worry at the day 14 mark for ms syringes.
2- sure. Better yet, start another batch now. Not making the same mistake.
3- Loose, tight, whatever. Indirect sunlight is beneficial at all stages. Store them higher in the room where less air is disturbed by traffic and away from fabrics which may harbor things you don't want to get in your jars.
-------------------- My Old Grow Logs
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awakendone


Registered: 08/05/10
Posts: 824
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Re: Concerned Pf Tek grower + wall of text = help please? <3 [Re: Wing]
#14593358 - 06/10/11 10:45 PM (12 years, 7 months ago) |
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so that tape is covering the holes? if so, you need to get it off. it's the wrong tape anyways. i never used any tape on my jars
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Wing
The Eye Tyrant



Registered: 04/25/11
Posts: 3,293
Last seen: 5 months, 11 days
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Re: Concerned Pf Tek grower + wall of text = help please? <3 [Re: awakendone]
#14593368 - 06/10/11 10:47 PM (12 years, 7 months ago) |
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If you have a dry vermiculite layer then you don't need tape. But if you dont't have a dry verm layer then you need micrpore tape.
-------------------- My Old Grow Logs
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VaeVictum
Sativa Cyborg

Registered: 06/01/11
Posts: 4,397
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Re: Concerned Pf Tek grower + wall of text = help please? <3 [Re: Wing]
#14593753 - 06/11/11 12:28 AM (12 years, 7 months ago) |
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Quote:
winged_1 said: If you have a dry vermiculite layer then you don't need tape. But if you dont't have a dry verm layer then you need micrpore tape.
I removed the tape since I do have the layer of vermiculite. I moved it to the top of my cupboards. Thanks for all the info.
Edited by VaeVictum (06/11/11 12:29 AM)
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DoubleG909



Registered: 05/08/11
Posts: 136
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Re: Concerned Pf Tek grower + wall of text = help please? <3 [Re: VaeVictum]
#14593862 - 06/11/11 01:02 AM (12 years, 7 months ago) |
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It's not the best picture, but your sub does look pretty coarse. The Idea behind the sub prep is to mix the water with the verm til the verm is at field capacity, then introduce the brown rice flour to the mix thus giving and even coating of brf to all the little verm particles. It won't coat the verm unless it's a flour and thats important because that creates a nice bridge for the myc to work its way around on. Try to post some better shots.
-------------------- “Nature is not our enemy, to be raped and conquered. Nature is ourselves, to be cherished and explored.” ― Terence McKenna
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VaeVictum
Sativa Cyborg

Registered: 06/01/11
Posts: 4,397
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Re: Concerned Pf Tek grower + wall of text = help please? <3 [Re: DoubleG909]
#14593944 - 06/11/11 01:29 AM (12 years, 7 months ago) |
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Quote:
DoubleG909 said: It's not the best picture, but your sub does look pretty coarse. The Idea behind the sub prep is to mix the water with the verm til the verm is at field capacity, then introduce the brown rice flour to the mix thus giving and even coating of brf to all the little verm particles. It won't coat the verm unless it's a flour and thats important because that creates a nice bridge for the myc to work its way around on. Try to post some better shots.
Here's a head on pic of one of the jars. This one is inoculated with the cambodia strain.

I might take a picture of the brf I made too but I'm lazy atm. Honestly the brf and verm we're probably 3/4 fine 1/4 coarse which shouldn't be that bad.
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DoubleG909



Registered: 05/08/11
Posts: 136
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Re: Concerned Pf Tek grower + wall of text = help please? <3 [Re: VaeVictum]
#14593992 - 06/11/11 01:44 AM (12 years, 7 months ago) |
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doesn't look too bad. Where did you get your spores from? Can you describe what your syringes looked like? Light, dark, light with dark clusters floating in it?
-------------------- “Nature is not our enemy, to be raped and conquered. Nature is ourselves, to be cherished and explored.” ― Terence McKenna
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VaeVictum
Sativa Cyborg

Registered: 06/01/11
Posts: 4,397
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Re: Concerned Pf Tek grower + wall of text = help please? <3 [Re: DoubleG909]
#14593995 - 06/11/11 01:46 AM (12 years, 7 months ago) |
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Quote:
DoubleG909 said: doesn't look too bad. Where did you get your spores from? Can you describe what your syringes looked like? Light, dark, light with dark clusters floating in it?
Light with dark clusters that I shook up until they became retarded. I got them from lilshopofspores.com
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