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OfflineAlbanian_agi
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Registered: 06/02/11
Posts: 11
Last seen: 11 years, 19 days
injecting my 1/2 pint jars (question)
    #14589647 - 06/10/11 09:51 AM (12 years, 8 months ago)

well i have about 10 jars with 4 holes on the top with tape on them and ready to inject.
my question is how do i go about injecting the jars?
how much do i inject in each jar/hole ?
also i bought a 12cc syringe but some solution got out and i only have 10/11 cc left is that enough?

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Invisiblejbbishop
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Registered: 04/16/11
Posts: 54
Re: injecting my 1/2 pint jars (question) [Re: Albanian_agi]
    #14589677 - 06/10/11 10:02 AM (12 years, 8 months ago)

A total of about 1-1.5 CC per jar.  If you're careful you can do all 10.  Wrap your index finger around the base of the plunger and you can feel more precisely what amount you're injecting as opposed to just the feeling you would get from having your thumb on the plunger, and this will give you additional control.  Insert the needle into the side of the jar and as you inject, pull back slightly to avoid plugging or you might end up wasting a bunch if you apply too much pressure because the needle is plugged.  Remember to stick the tip of the needle in your flame and pull back a little when it glows red hot to draw in some sterile air and then shake it up good to break up and distribute the spores evenly.  When they've been hydrated for a long time they clump up a lot more.  If you made your own syringes you'd only want to hydrate them for about a day and the sooner you use them the better.  If you would use any inoculation hood you wouldn't need to poke holes in your lids.  If you want to be more miserly you can inject 3 points but it will take longer to grow out.  In my experience it is more profitable to get a good print and make up to about 10 syringes from that.  The syringes often don't avoid contams for the two months they are supposed to be good for and half the syringes I've ever bought had already been contaminated.  Spore prints can last 2 years or longer.

Edited by jbbishop (06/10/11 10:31 AM)

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Offlinem_jacobs
Man Whore
Registered: 03/11/11
Posts: 121
Loc: CA
Last seen: 10 years, 8 months
Re: injecting my 1/2 pint jars (question) [Re: Albanian_agi]
    #14589711 - 06/10/11 10:08 AM (12 years, 8 months ago)

(Done inside a glove box.)
Assuming you left the tinfoil on top of the lids.
I remove the tinfoil.
I wipe the top of the lid/tape with alchol.
I flame sterilize the needle.
Then take the syringe and push it through the tape.
I inject in on the side of the jar.  Basically I do the smallest little dibble.  A little goes a long way.  No more then .5cc per hole.  So the jar alone will get a total of 2cc (4x .5cc = 2cc).  I did a lot less then 2cc when I did mine.

If you have only 1 syringe and u need 20cc, you can do half your jars, and then suck in more "STERILE" water into your syringe, but you have to make dam sure you using sterile water and procedures, or you will contaminate your syringe.


--------------------
Where I got my supplies

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OfflineAlbanian_agi
Stranger

Registered: 06/02/11
Posts: 11
Last seen: 11 years, 19 days
Re: injecting my 1/2 pint jars (question) [Re: m_jacobs]
    #14589868 - 06/10/11 10:38 AM (12 years, 8 months ago)

ahh thanks alot guys :laugh:!!
but m_jacobs i dont really want to put in water because atm i dont have any sterile water lol

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OfflineAlbanian_agi
Stranger

Registered: 06/02/11
Posts: 11
Last seen: 11 years, 19 days
Re: injecting my 1/2 pint jars (question) [Re: Albanian_agi]
    #14590169 - 06/10/11 11:47 AM (12 years, 8 months ago)

ok so i just injected them. had a little problem at first, the needle wouldent go red for me. But after some trial and error i got it to work.

so my question now is ...does it matter that some dark shit build up on the needle.
and i think i might have wasted a little of the the solution by the holes on the lid i ended up whipping the lids with alcohol.

anything wrong here??

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