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Offlinelionsman
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V. bombycina grow * 5
    #14578487 - 06/08/11 07:06 AM (12 years, 8 months ago)

I found V. bombycina for the first time last September as two waterlogged and mature fruitbodies growing on a well rotted horse chestnut bough on the ground.


I attempted to clone it, but from 10 plates, mycelium grew out from only one, where tissue had been taken from near the base of the stem. I went back to the same spot a couple of weeks later and found 2 perfect eggs which cloned easily.


At the end of last year I made spawn on wheat (wedge transfer in jars) and inoculated a couple of oak sawdust/bran/gypsum blocks. The mycelium did not appear to like growing on this substrate, providing a poorly mycellated block. It did produce a few small eggs on the surface, but the temperature in my grow room got too low and they aborted.
V. bom appears to have been grown for the first time in 1982 by a German group, who used newspaper as substrate. V. volvacea is grown commercially on cotton waste, suggesting that the volvariellas may be happiest on a simple cellulose substrate, rather than green sawdust.  Carsten (Mycelio) also found that V. bom likes growing on straw. I inoculated 4 bags with wheat spawn, just straw, straw plus bran, newspaper/straw/bran and newspaper/spent K. mutabilis substrate/bran. The straw/bran bag contaminated, but the other 3 colonised well, with eggs forming in the sealed bags after 3-4 weeks. The bags were opened up to allow the eggs to develop.


In the straw containing bags only one large egg grew,


but newspaper/Km/bran was a much more productive substrate, with about a dozen eggs forming.


They all hatched at pretty much the same time.


As the mushrooms reached maturity, their weight pulled the block apart. They are probably the most beautiful and delicious mushrooms I’ve grown.

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OfflineFeelers
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Re: V. bombycina grow [Re: lionsman] * 1
    #14579507 - 06/08/11 12:19 PM (12 years, 8 months ago)

That post was awesome. Fantastic grow and writeup. The eggs look almost like giraffe skin. :grin:

Can you please go into more detail about the taste? I'm quite intrigued.

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OfflineMycelio
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Re: V. bombycina grow [Re: lionsman]
    #14585153 - 06/09/11 11:52 AM (12 years, 8 months ago)

Hey Lionsman,

wow, you made it, this is fantastic news!
Is it OK for you, if I add mycelium pictures?

Carsten

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Invisiblelipa

Registered: 07/24/07
Posts: 2,684
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Re: V. bombycina grow [Re: Mycelio]
    #14585274 - 06/09/11 12:20 PM (12 years, 8 months ago)



Thats awesome!! Nice job.

I wanna taste some!!

Lipa

Edited by lipa (06/09/11 12:22 PM)

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Offlineragadinks
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Re: V. bombycina grow [Re: lionsman]
    #14585657 - 06/09/11 01:45 PM (12 years, 8 months ago)

Hey - really great news (that even awakened me from my sleep :wink: ) !!
You probably should send some mycelium to other people, so that it does not get lost, because I am not sure if every wild strain is 'well-behaved' as yours.

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Offlinelionsman
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Re: V. bombycina grow [Re: ragadinks]
    #14589195 - 06/10/11 06:47 AM (12 years, 8 months ago)

Thanks guys, Feelers, not sure how to describe the taste. The closest I could think of is M. procera, which is my favourite edible, it's not as good, but the closest I can think of. Carsten, add anything you like. Ragadinks, I will probably give this away to anyone who wants it. I've been giving a lot of stuff away recently and need a rest, but I'll run some plates out and post again when I have wedges.

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OfflineMike711
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Re: V. bombycina grow [Re: lionsman]
    #14589232 - 06/10/11 07:14 AM (12 years, 8 months ago)

Jealous, thats what its all about, Ive wanted to do the same type of thing but Ive just been to lazy.  whats your next wild clone project you going to try next

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Offlinelionsman
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Re: V. bombycina grow [Re: Mike711]
    #14589243 - 06/10/11 07:19 AM (12 years, 8 months ago)

Hi Mike, yeh I enjoy growing wierd shit, makes life interesting. I have some L. decastes growing which I will post soon, and 1 L. nuda growing (as in 1 mushroom!) from the 6 blocks I inoculated. I'll post it when it's ready.

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Invisiblepilz-kultur
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Re: V. bombycina grow [Re: lionsman]
    #14589346 - 06/10/11 08:07 AM (12 years, 8 months ago)

@lionsman
Really great Job ! – Very well done !

Some more details…Temp.-Hum.- Light ? …..would be great !
Could you notice primordia (Fruitbody) formation on your
Agar-Medium…?

…at least , I´m highly interested on a culture…maybe trading/exchange (Also like growing exotic stuff – Some on stock !) ??? Just PM me….

Greetings from Vienna
Walter


--------------------
We must be mushrooms , for they keep us in the dark and feed us dung !


www.pilz-kultur.at

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OfflineMycelio
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Re: V. bombycina grow [Re: pilz-kultur]
    #14589416 - 06/10/11 08:27 AM (12 years, 8 months ago)

I can hook up Walter, but not everybody, who is interested.

How about one or two volunteers, who are good in agar work, to spread the culture, so Lionsman won't have too much hassle?

Carsten

Edited by Mycelio (06/10/11 08:44 AM)

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Invisiblepilz-kultur
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Re: V. bombycina grow [Re: Mycelio]
    #14589615 - 06/10/11 09:39 AM (12 years, 8 months ago)

Hi Carsten and "Thank`s" !:wink:

Walter


--------------------
We must be mushrooms , for they keep us in the dark and feed us dung !


www.pilz-kultur.at

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Invisiblelipa

Registered: 07/24/07
Posts: 2,684
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Re: V. bombycina grow [Re: Mycelio] * 1
    #14589991 - 06/10/11 11:06 AM (12 years, 8 months ago)

Send me a plate one of you guys. I will make it available in the US. I will even fruit it out "big time" and distribute prints! This ones a keeper!!  It would be nice to have a print as well so I can get some new germ-plasm going. It's the perfect climate right now here in Southern California to grow these. I will also have a new cold storage unit specifically for warm weather varieties very soon.

By the way this mushroom is a homothallic species and has uni-nucleate haploid spores that are self fertile so they can produce fruitbodies from one spore. This makes things really easy in terms of producing mushrooms.

There is a lot of cool info on growing Volvariella on this website if you look around. http://www.nrcmushroom.org/

Lipa

Edited by lipa (06/10/11 11:18 AM)

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OfflineNeo Mithrandil
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Re: V. bombycina grow [Re: lipa]
    #14590571 - 06/10/11 01:16 PM (12 years, 8 months ago)

I added this species to the Bemushroomed mycobank. If one of you guys feels like creating an entry for lionsman's strain, that would be great.

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OfflineMycelio
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Re: V. bombycina grow [Re: Neo Mithrandil]
    #14591297 - 06/10/11 04:01 PM (12 years, 8 months ago)

Alright, here are some images:


Mycelium growing through beech sawdust with coffee grounds. This was my initial attempt, thinking if it grows on trees, it should like that substrate. Well, it does grow in, but slow and weak.

Now see what it does on some straw, which was lying on top:

In comparison, the mycelium almost explodes. This mushroom species is of the brown rot type, which means it can't digest lignin. So - as Lionsman wrote earlier - we better use something where the cellulose isn't fully embedded in lignin, like paper, straw or cotton waste (if available).

The mycelium is very similar to V. volvacea, it grows fast and branches often, almost in an 90° angle. Most times it grows high up in the air, just like cobweb or pin mold.
When colonization is complete (sometimes earlier) there is even more branching with short, swollen hyphae, like little balloons all around the hyphae. This is initially white, later becoming yellowish brownish. First time I saw it, I thought it was a sporulating mold.
Here is such a mature mycelium:

The smell is like V. volvacea, somehow sweet, somehow like sperm.

When trying around with straw substrates, I learned I could kill the mycelium with the levels of supplements I use for oysters. My best straw mixture was 90% straw, 5% coffee grounds and 5% rice. For soaking I used water from boiling potatoes (of course without salt). Then I got fast, dense growth and primordia often formed in large numbers even in small jars often before full colonization.

What else is important... lag period, moisture and yield. When being broken up and inoculated, the mycelium will rest for two or three days. This can be a problem when not working sterile, as molds have a higher chance to establish themselves.
In my experiments it appeared important, that the water content of the substrate was on the dry side, like 60 - 65%.
Compared to usual mushroom species, yield will be lower, as with all brown rot fungi. Seems like Lionsman got pretty close to the optimum already.

Carsten

Edited by Mycelio (06/14/11 01:26 PM)

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InvisibleJavadog
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Re: V. bombycina grow [Re: Mycelio]
    #14593586 - 06/10/11 11:35 PM (12 years, 8 months ago)

Beautiful grow!

My first Volvaria effort failed badly....what I had hoped
might turn out to be eggs didn't:


But hope springs eternal.

Take care, and thank you for sharing.

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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Invisiblebadman
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Re: V. bombycina grow [Re: lionsman] * 1
    #14595108 - 06/11/11 09:39 AM (12 years, 8 months ago)

Ar mate,

:aweoverdose:

What a fucking sweet grow.



POTM standardly

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Offlinelionsman
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Re: V. bombycina grow [Re: badman]
    #14604712 - 06/13/11 05:58 AM (12 years, 8 months ago)

Lipa, can you pm. me address. I'll send you a slant, it would be great if you can distribute it within the US.
Hi Walter, yes it forms small primordial eggs on agar after a while (MA). As far as I can tell it is a warmth loving mushroom. The weather has been erratic in the UK recently. Growth of the mushrooms themselves slowed when it got cool (teens C) and was pretty rapid when a few warm days hit us. The parent mushrooms were also found during warm wet weather.
Neo, I'm about to run some plates out and will list some wedges or slants on Bemushroomed when they're ready. Thanks for your comments guys.

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InvisibleFernandoCastro
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Re: V. bombycina grow [Re: lionsman]
    #14604867 - 06/13/11 07:35 AM (12 years, 8 months ago)

Quote:

lionsman said: Neo, I'm about to run some plates out and will list some wedges or slants on Bemushroomed when they're ready. Thanks for your comments guys.



Could you notify here when they're available in Bemushroomed?
That would be great.

Thanks in advance.


--------------------
www.cogusbox.com


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Invisibleloucal
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Re: V. bombycina grow [Re: FernandoCastro]
    #14610902 - 06/14/11 08:52 AM (12 years, 8 months ago)

This is amazing.  Pictures are awesome, thank you!

Also patiently awaiting this to hit bemushroomed.

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OfflineBlueLightRain
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Re: V. bombycina grow [Re: loucal]
    #14611239 - 06/14/11 10:22 AM (12 years, 8 months ago)

I agree, this is a wonderful grow. Beautiful photos, too. If anyone is the man to give it to, its Lipa. He'll do it well :smile:


--------------------
mushroomjoe.com - that's me!

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