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OfflineNeoSporen
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Registered: 09/05/09
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Pan foe microscopy pics.
    #14576658 - 06/07/11 08:54 PM (12 years, 8 months ago)

It's been a while since I've posted anything. Been working over 65 hours a week, so I don't really have any time to hunt, but I wanted to post some pics from the other day. I found some pan foes in my yard on sunday and just wanted to check them out under the scope. Spores measure an average of about 13.7 X 6.85 micrometers. No reticle yet, but I figured out how to measure distance using my software. :cheers: and happy hunting.

Gills and spores, 80X


Pleurocystidia at 200X


Pleurocystidia at 800X


Pleurocystidia at 800X


Spores 200X


Spores at 2000X


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Having lived through an existence close to nature, one accepts the small and simple things as most important in life. Sun, wind, rain and snow. The sounds birds make, smells of fresh wild flowers. Love of all kinds, from friends and family, thy self and our neighbors. Beautiful sunrises to the darkest clouds dancing above in the sky. To forgive, learn, share and express. This is the only thing a man such as myself can ask for. What comes as the result is nothing short of the core of human existence, to truly live free in body and mind.

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Offlinehyperjump69
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Re: Pan foe microscopy pics. [Re: NeoSporen]
    #14576677 - 06/07/11 08:57 PM (12 years, 8 months ago)

:thumbup:


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This link will drive you NUTS-------> http://www.youtube.com/watch?v=cKYw0XJfzO4&feature=BFa&list=LLimI46qikD80&index=4



Sorry I don't conform to your idea of reality. Now go away before I bitch-slap you.

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OfflineNeoSporen
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Re: Pan foe microscopy pics. [Re: hyperjump69]
    #14576717 - 06/07/11 09:03 PM (12 years, 8 months ago)

Wow, I didn't even notice that my notes are all wrong now. When did it change to Panaeolina foenisecii?


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Having lived through an existence close to nature, one accepts the small and simple things as most important in life. Sun, wind, rain and snow. The sounds birds make, smells of fresh wild flowers. Love of all kinds, from friends and family, thy self and our neighbors. Beautiful sunrises to the darkest clouds dancing above in the sky. To forgive, learn, share and express. This is the only thing a man such as myself can ask for. What comes as the result is nothing short of the core of human existence, to truly live free in body and mind.

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OfflineTwiztidsage
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Re: Pan foe microscopy pics. [Re: NeoSporen]
    #14576775 - 06/07/11 09:15 PM (12 years, 8 months ago)

Great micrographs!

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OfflineAlan RockefellerM
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Re: Pan foe microscopy pics. [Re: NeoSporen]
    #14576818 - 06/07/11 09:23 PM (12 years, 8 months ago)

Your pleurocystidia is cheilocystidia.  The subcapitate shape is a good match for Panaeolina foenisecii.

The spores are rough, which I can barely see in your spore pic, but looking at the other Panaeolina foenisecii spores on the web, few show the ornamentation.

Here is a picture I took in 2007, before I started adjusting the white balance:

http://commons.wikimedia.org/wiki/File:Panaeolina_foenisecii_spores_3.jpg

Here is one of Darvin's spore images taken with a $5000 phase contrast microscope and its still hard to see:

http://mushroomobserver.org/image/show_image/81309?

Easier to see on one of Oluna Ceska's drawings:



Can you see the ornamentation when looking through the eyepiece?  To maximize the chance set the condenser aperture to really small and adjust the condenser until they look best.

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OfflineNeoSporen
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Re: Pan foe microscopy pics. [Re: Alan Rockefeller]
    #14576853 - 06/07/11 09:30 PM (12 years, 8 months ago)

:lol: Thanks alan. the help is greatly sppreciated. Looking through the eyepiece, I can see it much better than the pictures show. It pretty much looks exactly like the drawing you provided. I get the gill face and edge mixed up, and while still learning, any pointers are awesome. I'm still playing around with the white balance and light levels, but I gave in and bought the phase contrast kit that fits my scope. I've been using it for many other things besides looking at fungi (moastly metallurgy) so it's money well spent in my mind.


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Having lived through an existence close to nature, one accepts the small and simple things as most important in life. Sun, wind, rain and snow. The sounds birds make, smells of fresh wild flowers. Love of all kinds, from friends and family, thy self and our neighbors. Beautiful sunrises to the darkest clouds dancing above in the sky. To forgive, learn, share and express. This is the only thing a man such as myself can ask for. What comes as the result is nothing short of the core of human existence, to truly live free in body and mind.

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OfflineAlan RockefellerM
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Re: Pan foe microscopy pics. [Re: NeoSporen]
    #14576946 - 06/07/11 09:49 PM (12 years, 8 months ago)

Quote:

bfogg8706 said:
I'm still playing around with the white balance and light levels, but I gave in and bought the phase contrast kit that fits my scope.




Oh that explains why the images look like that.  Its pretty cool how the normally clear cystidia show up pretty well.  You can also set the condenser to the wrong phase setting and get a darkfield effect which makes cystidia look really awesome, like ghosts.

Quote:

Looking through the eyepiece, I can see it much better than the pictures show. It pretty much looks exactly like the drawing you provided.




You might be able to get more resolution with a different camera - The amscope webcams are ok but lack dymanic range and good resolution.  I have been using a Canon S95 to take pics through the microscope and the pictures are much better than in the past when I was using older cameras with dirty lenses. 

If you use a handheld camera to take pictures through the scope, take care to never get dust in the lens (always keep the camera in a case).  When you use the camera to take regular pictures the dust doesn't matter because the light is coming in from many directions so it takes a huge piece of dust to make a spot on your picture.  But when taking pictures through the microscope all the light is coming from one direction so any tiny bit of dust is projected perfectly on to your micrograph.  Thousands of my micrographs have the same black mark in the same place from dust inside the camera lens.  A new Canon SD1300 runs $129 and would be a good choice for a microscope camera.

Quote:

I've been using it for many other things besides looking at fungi (moastly metallurgy) so it's money well spent in my mind.




How do you use it for metallurgy, what kinds of things can you see?

Since light can not pass through metal I guess you would need some other kind of illumination strategy.  I would either use epi-illumination or a little disc of tissue paper around the objective.  For epi-illumination, shine a bright light (usually LED) down one of the eyepieces that you are not taking the picture through, perhaps the trinocular if you have it, though if you can turn off the trinocular and shine the light through one of the binocular eyepieces that will give you more total light.

The girl who does the focus stacking uses a novel illumination method where she cuts a circle of tissue paper out and affixes it to the front of the objective, with a small hole for the lens, then reflects bright light off of that to get a diffuse light that comes from many angles.  That might give better light than epi-illumination, try both.

My friend just got a 50 watt white LED.  I am going to borrow it soon and see if it is useful for microscopy.  It is about as bright as a car headlight with the brights on.

To see the pleurocystidia on a fresh specimen, make an extremely thin transverse section of the cap with a new razor blade.  The section should look like a saw blade, with the cheilocystida on the tips of the teeth, pleurocystidia on the sides of the teeth, and pileipellis on the back of the blade.  Since it is nearly impossible to make a section thin enough, I angle the blade on purpose so one side is way too thick and the other side is impossibly thin.  Then I cut off the thick part, leaving a really good section.

With dried material it is harder for me, I guess the best way is to rehydrate and cut the gill into many tiny strips with a new razor blade, and look for pleurocystidia along the cuts.  I have mixed success doing that so I really prefer to do microscopy on fresh mushrooms, though others say its no problem or even easier sometimes to use dried material.

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InvisiblesporeRider
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Re: Pan foe microscopy pics. [Re: Alan Rockefeller]
    #14577202 - 06/07/11 10:28 PM (12 years, 8 months ago)

:bow2::awesomenod: Awesome bfogg:headbanger:
Man that looks like a fun toy you got:thumbup::thumbup:
Looking forward to LOTS of microscopy done by you with your awesome book full of finds:super::cool: Keep at it your already doing GGGRRRREAT:smile::shineon:


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