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OfflineMowglee
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Spawn bags. Open bag syringe.
    #14576459 - 06/07/11 08:17 PM (12 years, 7 months ago)

So, was just wondering if anyone else has just opened a freshly p.c. rye bag and just shot the wet spores from the syringe into the opening of the bag and sealed it.


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InvisibleeLShaMukO
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Re: Spawn bags. Open bag syringe. [Re: Mowglee] * 1
    #14576493 - 06/07/11 08:23 PM (12 years, 7 months ago)

you can stab the bag and put micro pore after that .
rub the spot whit alcohol , flame needle and stab the mofo.

what you say is possible,just more open to contaminants.


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OfflineMowglee
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Re: Spawn bags. Open bag syringe. [Re: eLShaMukO]
    #14576629 - 06/07/11 08:49 PM (12 years, 7 months ago)

How many CC's would you suggest for 5p of rye seed?  I'm going to try this. I like finding out where to cut corners.


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Offlineresptodd
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Re: Spawn bags. Open bag syringe. [Re: Mowglee]
    #14576661 - 06/07/11 08:55 PM (12 years, 7 months ago)

Poke the bag....... sterile needle. clean the site with alcohol. shake your syringe. inject 2cc. clean the site with alcohol. put a piece of tape on it.


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InvisibleeLShaMukO
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Re: Spawn bags. Open bag syringe. [Re: Mowglee]
    #14576701 - 06/07/11 09:01 PM (12 years, 7 months ago)

2 to 4 cc's is fine.


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OfflineMowglee
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Re: Spawn bags. Open bag syringe. [Re: resptodd]
    #14576712 - 06/07/11 09:02 PM (12 years, 7 months ago)

No disrespect to the flawless technique, but I'm just going to open the top and shoot 2cc's in there. =)


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InvisibleeLShaMukO
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Re: Spawn bags. Open bag syringe. [Re: Mowglee]
    #14576749 - 06/07/11 09:10 PM (12 years, 7 months ago)

good luck.


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OfflineWing
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Re: Spawn bags. Open bag syringe. [Re: Mowglee]
    #14576770 - 06/07/11 09:14 PM (12 years, 7 months ago)

Why risk it? You might be perfectly fine but its an unnecessary risk. You might as well not sterilize ur needle and just leave the bag open lol

Good luck man


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OfflineRogerRabbitM
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Re: Spawn bags. Open bag syringe. [Re: Mowglee]
    #14576798 - 06/07/11 09:19 PM (12 years, 7 months ago)

Quote:

Mowglee said:
No disrespect to the flawless technique, but I'm just going to open the top and shoot 2cc's in there. =)




Very bad idea unless you have a flowhood.
RR


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InvisibleDoctor_Inoc
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Re: Spawn bags. Open bag syringe. [Re: Mowglee]
    #14576842 - 06/07/11 09:27 PM (12 years, 7 months ago)

Quote:

Mowglee said:
No disrespect to the flawless technique, but I'm just going to open the top and shoot 2cc's in there. =)



Even if you'r inoculating in front of a flowhood, open bag inoculations with a spore syringe is impractical.  Save the open bag inoculations for inoculating with fully colonized grain spawn or agar cultures.  You'r asking for contamination opening your bag just to inject spore solution out a syringe. 

You best heed what the others are saying of first sealing the bag and inoculating through the bag, after sterilization.  Seal the puncture with instead of micro-pore tape, use ducting, scotch, electrical or masking.  Micropore-tape becomes saturated when you wipe it alcohol and opens up a vector for contaminates to be wicked in via, the wet tape.


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OfflineMowglee
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Re: Spawn bags. Open bag syringe. [Re: RogerRabbit]
    #14576845 - 06/07/11 09:29 PM (12 years, 7 months ago)

I normally just spoon myc. from a quart jar into the bag to inoculate them (in this room; and use bleach on my spoon as well as other sterilization tech. for the room). The only problem I ever had was using an infected bag of myc. I've also moved away from using the impulse sealer. I just grab the top of the bag, spin it, fold it, and zip tie it. It has worked for over 100+ bags. Are the wet spores really more prone to contamination?


You guys have freaked me into first shooting it in to inoculate quart sized jars. I guess I'll try 6 out, and 1 bag.  Let's see what happens. =P 

Pictures in a couple weeks.


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OfflineWing
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Re: Spawn bags. Open bag syringe. [Re: Mowglee]
    #14577263 - 06/07/11 10:37 PM (12 years, 7 months ago)

Go with what works for you man. If you've been doing it your way and not had any problems then keep at it. I would personally take every precaution available.


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