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OfflineCanadian Bud
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Other ways to reproduce mushrooms without making spore print print
    #14575190 - 06/07/11 03:52 PM (12 years, 8 months ago)

I am just beginning my research on growing mushrooms, but was wondering if you can reproduce mushrooms without making a spore print. What are other methods that you can do?

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InvisibleTranscendingLife
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Re: Other ways to reproduce mushrooms without making spore print print [Re: Canadian Bud]
    #14575200 - 06/07/11 03:54 PM (12 years, 8 months ago)

LC, GLC, clone, iso on agar, G2G...

if I'm missing something someone else please chime in...


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OfflineHashishin13
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Re: Other ways to reproduce mushrooms without making spore print print [Re: TranscendingLife]
    #14575774 - 06/07/11 06:00 PM (12 years, 8 months ago)

Whats GLC? Grain liquid culture?

Also can you lop off a bit of mushroom and throw it into a jar? I thought u had to go through agar first?

I think you got all of them, for cubes at least. Woodlovers can do cardboard spawn, which is kinda a division of LC, G2G and cloning.


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Offlinemabachko
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Re: Other ways to reproduce mushrooms without making spore print print [Re: Hashishin13]
    #14575795 - 06/07/11 06:05 PM (12 years, 8 months ago)

Use the search, that is what it is there for, then ask questions about the procedure.

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Offlinepunkrocker292004
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Re: Other ways to reproduce mushrooms without making spore print print [Re: mabachko]
    #14575803 - 06/07/11 06:08 PM (12 years, 8 months ago)

TITTIES!!!!!!!!!!!!


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OfflineRogerRabbitM
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Re: Other ways to reproduce mushrooms without making spore print print [Re: punkrocker292004]
    #14575814 - 06/07/11 06:10 PM (12 years, 8 months ago)



--------------------
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Invisiblejokefox
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Re: Other ways to reproduce mushrooms without making spore print print [Re: TranscendingLife]
    #14575830 - 06/07/11 06:12 PM (12 years, 8 months ago)

Quote:

todlow said:
LC, GLC, clone, iso on agar, G2G...

if I'm missing something someone else please chime in...





so what tl are we not friends anymore
you forgot agar broth

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OfflineMatty_Rulez
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Re: Other ways to reproduce mushrooms without making spore print print [Re: Canadian Bud]
    #14575839 - 06/07/11 06:14 PM (12 years, 8 months ago)

I'll take cloning for $200, Alex...


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Invisiblebbox244
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Re: Other ways to reproduce mushrooms without making spore print print [Re: Hashishin13]
    #14575849 - 06/07/11 06:16 PM (12 years, 8 months ago)

For cloning, I tear the mushroom in half and using my scalpel, I take a rice sized sample from the base of the stem and transfer to agar, no h202,etc.  I usually make 2 tri-section plates of my clone.  I take the cleanest section and make another tri-section plate from that to get my wedges.  Then I do the following for my GLC...

I gave up trying regular LC's and only do WBS GLC's now.  I take WBS and soak it for 24 hours in 1/3 coffee, gypsum and hydrated lime (to offset PH from coffee).  Then I simmer the WBS in the soaking solution for 10 minutes, and steam strain like in RR's vids.  I let them sit in the strainer until I can take a small hand full of WBS and put it on toilet paper for 10 seconds without it wetting the paper.  Then I very loosely load narrow half pint, or pint jars half way up, put on my lid (I use RTV and RTV'd Tyvek squares), cover with foil and PC at 15 psi for 60 min.  Let cool naturally, then I noc with an agar wedge.  After 30-40% colonization, I give them a good shake.  Then they are done 2-3 days later.  At that point, I inject sterile H20, shake, suck back up and noc up what ever I am inoculating at that point.  Works every time, the WBS is so good, I won't even bother with anything else now.  Even on MS nocs, you'd be amazed at how fast loose WBS colonizes.  It's actually strange.  A lot of times, the WBS jar will re-colonize after taking a GLC sample from it if you use really long needles and suck out all the water you can.


--------------------
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OfflineCanadian Bud
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Re: Other ways to reproduce mushrooms without making spore print print [Re: bbox244]
    #14576183 - 06/07/11 07:26 PM (12 years, 8 months ago)

Go to 46:15 in this video:


He uses one jar colonized with mycelium to inoculate ten other jars. Is this the most popular method of reproduction? Do you guys prefer to use rye grain as the substrate because in the video the first method he uses with vermiculite, brown rice flour and water does not allow you to reproduce by mixing it into other jars like with the rye grain.

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InvisibleTranscendingLife
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Re: Other ways to reproduce mushrooms without making spore print print [Re: jokefox]
    #14576872 - 06/07/11 09:33 PM (12 years, 8 months ago)

Quote:

jokefox said:
Quote:

todlow said:
LC, GLC, clone, iso on agar, G2G...

if I'm missing something someone else please chime in...





so what tl are we not friends anymore
you forgot agar broth




we're still friends :hug: agar broth = LC or GLC...


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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Invisiblejokefox
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Re: Other ways to reproduce mushrooms without making spore print print [Re: TranscendingLife]
    #14576914 - 06/07/11 09:44 PM (12 years, 8 months ago)

:durrhurr:

so i think thats all them then

unless you count mycelium in sterile water,

i wonder if someone could come up with something else new

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InvisibleTranscendingLife
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Re: Other ways to reproduce mushrooms without making spore print print [Re: jokefox]
    #14576932 - 06/07/11 09:47 PM (12 years, 8 months ago)

Quote:

jokefox said:
:durrhurr:

so i think thats all them then

unless you count mycelium in sterile water,

i wonder if someone could come up with something else new




isn't that GLC? :confused2:...

I've heard of spawning bulk to bulk before, but never recommend it as that's really touchy & a waste of time/effort IMHO.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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InvisibleDoctor_Inoc
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Re: Other ways to reproduce mushrooms without making spore print print [Re: jokefox]
    #14576933 - 06/07/11 09:47 PM (12 years, 8 months ago)

A slurry.

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Invisiblejokefox
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Re: Other ways to reproduce mushrooms without making spore print print [Re: Doctor_Inoc]
    #14576953 - 06/07/11 09:50 PM (12 years, 8 months ago)

so what, i dont know all the terms still :datass:

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OfflineCanadian Bud
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Re: Other ways to reproduce mushrooms without making spore print print [Re: jokefox]
    #14577042 - 06/07/11 10:03 PM (12 years, 8 months ago)

Can you answer the questions I asked in my second post?

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InvisibleDoctor_Inoc
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Re: Other ways to reproduce mushrooms without making spore print print [Re: Canadian Bud]
    #14577064 - 06/07/11 10:06 PM (12 years, 8 months ago)

Quote:

He uses one jar colonized with mycelium to inoculate ten other jars. Is this the most popular method of reproduction? Do you guys prefer to use rye grain as the substrate because in the video the first method he uses with vermiculite, brown rice flour and water does not allow you to reproduce by mixing it into other jars like with the rye grain.



That's called grain to grain transfer.  Personally, grain to grain tranfers is my favorite method of inocualting more grain mediums. 

Use WBS or rye.

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InvisibleTranscendingLife
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Re: Other ways to reproduce mushrooms without making spore print print [Re: Doctor_Inoc]
    #14577077 - 06/07/11 10:08 PM (12 years, 8 months ago)

that video is of a Grain to Grain (G2G).

That's how we make 1 jar equal 10 equal 100 equal 1000...
Quote:

Doctor_Inoc said:
Quote:

He uses one jar colonized with mycelium to inoculate ten other jars. Is this the most popular method of reproduction? Do you guys prefer to use rye grain as the substrate because in the video the first method he uses with vermiculite, brown rice flour and water does not allow you to reproduce by mixing it into other jars like with the rye grain.



That's called grain to grain transfer.  Personally, grain to grain tranfers is my favorite method of inocualting more grain mediums. 

Use WBS or rye.




QFT


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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Invisiblejokefox
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Re: Other ways to reproduce mushrooms without making spore print print [Re: Doctor_Inoc]
    #14577080 - 06/07/11 10:08 PM (12 years, 8 months ago)

i like agar broth
cause i made to much of it and i have no choice :fonz:

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Invisiblestonesun
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Re: Other ways to reproduce mushrooms without making spore print print [Re: Canadian Bud]
    #14577103 - 06/07/11 10:12 PM (12 years, 8 months ago)

Quote:

Canadian Bud said:
Can you answer the questions I asked in my second post?



Quote:

Hashishin13 said:
can you lop off a bit of mushroom and throw it into a jar?





Sure, you can clone that way too.
http://www.shroomery.org/forums/showflat.php/Number/14334911#14334911

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