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OfflineGigabyt3r
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Oops? Hopefully not too late?
    #14573331 - 06/07/11 08:46 AM (12 years, 7 months ago)

I just read that after inoculation you're supposed to leave all the 4 holes in each jar uncovered?

I covered mine up with tape due to contams!

Can I get away with this or do I need to uncover?


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"Ummm, you have to be a run-of-the-mill dipshit to use popcorn as your substrate, and sterilize it in the microwave"

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Offlineozzysmygod
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Re: Oops? Hopefully not too late? [Re: Gigabyt3r]
    #14573339 - 06/07/11 08:50 AM (12 years, 7 months ago)

Do you have a dry layer of vermiculite over your BRF mix?


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OfflineMushroomPalace
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Re: Oops? Hopefully not too late? [Re: Gigabyt3r]
    #14573358 - 06/07/11 08:57 AM (12 years, 7 months ago)

You should cover them with micropore tape.
Leaving them uncovered is absolute bullsh*t!
Micropore tape or filter discs or tyvek. That's the way to go. Who told you to leave them uncovered?


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OfflineWing
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Re: Oops? Hopefully not too late? [Re: MushroomPalace]
    #14573417 - 06/07/11 09:17 AM (12 years, 7 months ago)

You ae perfectly fine if you have a DRY layer of vermiculite. But it has to be about an inch deep and DRY. If you dont feel comfortable with just a dry verm layer then micropore take is ok.


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OfflineMushroomPalace
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Re: Oops? Hopefully not too late? [Re: Wing]
    #14573427 - 06/07/11 09:21 AM (12 years, 7 months ago)

I feel that it's always better to play safe and add the micropore tape.

Specially when you're a beginner in mycology, it's better to be safe than sorrow.


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OfflineSomeGuy
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Re: Oops? Hopefully not too late? [Re: Wing]
    #14573431 - 06/07/11 09:22 AM (12 years, 7 months ago)

I've never used micropore tape in my life. You haven't ruined them yet, but do uncover the holes, or you will. They can go a couple of weeks from inoculation before it becomes a serious issue. Just remember; DON"T shake, jostle, flip, or turn them sideways. They need to stay upright at all times, I promise


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Offlinesteelmonkey
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Re: Oops? Hopefully not too late? [Re: SomeGuy]
    #14573583 - 06/07/11 10:02 AM (12 years, 7 months ago)

Why is this going around that its ok to leave the inoculation holes uncovered,the dry vemiculites is a deterent to contams not a guarantee.If you leave your holes open your playing russian roulette


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Offlinenorml56
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Re: Oops? Hopefully not too late? [Re: steelmonkey]
    #14573649 - 06/07/11 10:16 AM (12 years, 7 months ago)

I leave mine uncovered and just cut out squares of tyvek an unscrew the ring on the jar an lay the tyvek over the top an screw it back down. That and the dry layer of verm has been a good success. Plus I only looked at the jars maybe 3 times during colonization.


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OfflineSomeGuy
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Re: Oops? Hopefully not too late? [Re: steelmonkey]
    #14573654 - 06/07/11 10:17 AM (12 years, 7 months ago)

Quote:

steelmonkey said:
Why is this going around that its ok to leave the inoculation holes uncovered,the dry vemiculites is a deterent to contams not a guarantee.If you leave your holes open your playing russian roulette



let me guess, your girls on birth control and still makes you wear condoms?


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Offlinesteelmonkey
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Re: Oops? Hopefully not too late? [Re: SomeGuy]
    #14573731 - 06/07/11 10:33 AM (12 years, 7 months ago)

Come again?


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OfflineMushroomPalace
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Re: Oops? Hopefully not too late? [Re: steelmonkey]
    #14573735 - 06/07/11 10:34 AM (12 years, 7 months ago)

Quote:

steelmonkey said:
Why is this going around that its ok to leave the inoculation holes uncovered,the dry vemiculites is a deterent to contams not a guarantee.If you leave your holes open your playing russian roulette



:whathesaid:


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OfflineMushroomPalace
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Re: Oops? Hopefully not too late? [Re: Gigabyt3r]
    #14573788 - 06/07/11 10:49 AM (12 years, 7 months ago)

Original poster:
It could be that you'll be fine by leaving the holes uncovered, but then you're just lucky.
The standard says that you need to cover your holes with some sort of barrier that is able to stop contaminant growth. What it is, doesn't matter. Whether it's tyvek, polyfill, micropore tape, filter discs,... it doesn't matter. But a sole barrier of vermiculite isn't enough.
A piece of vermiculite is about... 1mm? 2mm?
Well, fungi spores (of Trichoderma for example) are about 10µm - 12µm.
You see how contaminant spores could easily reach your wet substrate and germinate.

You DO need some protection against contaminants


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OfflinePerun
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Re: Oops? Hopefully not too late? [Re: steelmonkey]
    #14573805 - 06/07/11 10:53 AM (12 years, 7 months ago)

Quote:

steelmonkey said:
Why is this going around that its ok to leave the inoculation holes uncovered,the dry vemiculites is a deterent to contams not a guarantee.If you leave your holes open your playing russian roulette




You need to leave inoculation holes uncovered for GE...dry verm layer is your filter! You can use micropore tape,but in general...there is no need!


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First:                                                        Then:


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Offlinesteelmonkey
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Re: Oops? Hopefully not too late? [Re: Perun]
    #14573832 - 06/07/11 11:00 AM (12 years, 7 months ago)

Quote:

Perun said:
Quote:

steelmonkey said:
Why is this going around that its ok to leave the inoculation holes uncovered,the dry vemiculites is a deterent to contams not a guarantee.If you leave your holes open your playing russian roulette




You need to leave inoculation holes uncovered for GE...dry verm layer is your filter! You can use micropore tape,but in general...there is no need!





Thats why you use tyvek patches or poly in your lids


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OfflineMushroomPalace
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Re: Oops? Hopefully not too late? [Re: steelmonkey]
    #14574020 - 06/07/11 11:41 AM (12 years, 7 months ago)

Quote:

steelmonkey said:
Quote:

Perun said:
Quote:

steelmonkey said:
Why is this going around that its ok to leave the inoculation holes uncovered,the dry vemiculites is a deterent to contams not a guarantee.If you leave your holes open your playing russian roulette




You need to leave inoculation holes uncovered for GE...dry verm layer is your filter! You can use micropore tape,but in general...there is no need!





Thats why you use tyvek patches or poly in your lids



Indeed. As I said earlier, contaminant spores get through your vermiculite barrier vary easily.
Micropore tape prevents this while allowing gas exchange (GE).


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OfflineSomeGuy
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Re: Oops? Hopefully not too late? [Re: MushroomPalace]
    #14574049 - 06/07/11 11:48 AM (12 years, 7 months ago)

WRONG!!!!!!!!!!!!!!! contaminant spores don't have a shovel. The original pf didn't call for tyvek or micropore tape


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Offlinesteelmonkey
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Re: Oops? Hopefully not too late? [Re: SomeGuy]
    #14574079 - 06/07/11 11:54 AM (12 years, 7 months ago)

So everyone varied from the old PF tek and started using tyvek and poly for what reason?


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Offlineozzysmygod
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Re: Oops? Hopefully not too late? [Re: steelmonkey]
    #14574111 - 06/07/11 11:59 AM (12 years, 7 months ago)

Without an inch of dry verm ,you can fit an inch more BRF :p


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OfflineMushroomPalace
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Re: Oops? Hopefully not too late? [Re: SomeGuy]
    #14574148 - 06/07/11 12:06 PM (12 years, 7 months ago)

If you leave your jars in a place without air circulation and don't touch your jars during colonization, than indeed, you could be fine.

Although I think that the PF tek has been modified for a reason. We now use micropore tape and filter disks.
Paul Stamets uses jars and plastic bags with filter patches in his books "The Mushroom Cultivator" and "Mycelium Running" and in his laboratory of fungi perfecti.
Marc Keith uses filter discs in his DVD series "Let's Grow Mushrooms!".
Every myco-supplier sells jars with filter discs, bags with filter patches, even jars with lids with filters and self healing injection ports.
I think it's a bit primitive to work without it.
On the other hand, I've said it a thousand times and I'll say it again: everybody has their own way of working.
If it works for you, than great! But be fair, most beginning growers (and a certain amount of the experienced growers) check their mushrooms and substrates every day and they'll probably pick their jars up. By doing this you create air circulation and movement which makes it possible for contaminants to enter your jar.


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OfflineSomeGuy
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Re: Oops? Hopefully not too late? [Re: MushroomPalace]
    #14574161 - 06/07/11 12:10 PM (12 years, 7 months ago)

the whole filter disks and inoculation ports are for grain. You can't do grain pf style because you wouldn't be able to shake it. It would take one hell of a typhoon to move your verm. layer through those 4 little tiny holes. it's redundant to use more than one air filtration method, but it wouldn't hurt anything


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