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Fungal growth
Lootinint



Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
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whatman syringe filters, please explain!
#14561097 - 06/04/11 05:48 PM (12 years, 8 months ago) |
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they look pretty cool, but how does that tiny needle hole provide enough ge? i realize lc's don't need much, but if 1/4 inch is the norm, how does a wheel filter even come close? and if it does, do lc's even need the ge at all, provided the jar is 3/4 or less full?
Edited by Fungal growth (06/05/11 10:53 AM)
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Are you talking about Whatmans?
LCs don't need GE.
You need GE port for LCs so you can pull the liquid into your syringe, cuz if it wasn't there, you'd have a vacuum. Thus, it would be impossible to extract the LC.
LCs do not require GE, because it's in liquid, but I feel it's beneficial for LCs to have a bit of GE.
See my Lid tek...I use Whatmans for agar dishes & LCs...
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Quote:
todlow said: LCs do not require GE, because it's in liquid, but I feel it's beneficial for LCs to have a bit of GE.
You still need the same amount of oxygen for the myc though. There's some in the jar of course, and if you leave a lot of room that helps too. Completely agree the biggest issue is equalizing pressure.
-------------------- You make it all possible. Doesn't it feel good?
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Fungal growth
Lootinint



Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
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Re: wheel filters, please explain! [Re: Doc_T]
#14564109 - 06/05/11 10:50 AM (12 years, 8 months ago) |
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yes i mean whatmans, syringe filters. sorry, the term 'wheel filter' is a leftover from my days in junkie hell. ok, so they're main use is for equalizing pressure in lc's, not necessarily ge. would'nt work on a grain jar then.
another mildly related question, i don't know myself but i've read that most contaminate spores are larger than cubensis spores. with the proper micron size, could a syringe filter be used as an extra precaution when innoculating with spores, filtering out the trich and the cob while letting the cube by?
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Won't work on grain jars, correct. I like polyfil for grain jars.
Your second question is just wrong from the get-go, you don't shoot with a dirty syringe.
-------------------- You make it all possible. Doesn't it feel good?
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Fungal growth
Lootinint



Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
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Re: wheel filters, please explain! [Re: Doc_T]
#14564526 - 06/05/11 12:33 PM (12 years, 8 months ago) |
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i have a few sfd's but when they're all in use i use poly.
right, but as no spore print is 100% sterile, no syringe can be either. agar is tried and true, but there may be other ways not found yet. but sadly, a little research turned out that cubensis spores are considerably larger than trichoderma spores.
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Mycowlogist
Freethinker



Registered: 06/29/08
Posts: 534
Loc: in a galaxy far far away....
Last seen: 10 years, 1 month
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If you have an syringe port. Why don't you sterilize a syringe stuffed with polyfill (without the plunger) and use it as a filter/equalizer. Considering you have enough room in your syringe port for the 2 syringes. When you don't need it any more remove the syringe filter.
If that makes sense?
-------------------- "And in the end, it's not the years in your life that count. It's the life in your years." Honest Abe
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: wheel filters, please explain! [Re: Mycowlogist]
#14566801 - 06/05/11 10:02 PM (12 years, 8 months ago) |
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spores are larger than .2um.
Won't work to use it as a filter like that.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Fungal growth
Lootinint



Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
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Quote:
todlow said: spores are larger than .2um.
Won't work to use it as a filter like that.
no, it won't. the #'s i found show cube spores are considerably larger than trich spores. other spores run the gamut from .1um to 100um.
do you think the whatman's are superior than sfd's or poly? kind of a moot point i guess since my contam rate hovers right around zero.
Edited by Fungal growth (06/06/11 11:20 AM)
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Whatmans are the same um as SFDs, as long as you get the right ones.
Both should be .2 um.
Whatmans just have different applications than SFDs
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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