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OfflineYSJ
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Registered: 01/31/03
Posts: 57
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Something which is elementary, but which may prove useful... * 3
    #1456862 - 04/14/03 01:14 PM (14 years, 15 days ago)

Admins/Mods: I wasn't quite sure where to post this, so figured this was as good a place as any, as those suffering the woes of contams should find it helpful...In any case, please feel free to move this thread (and/or to copy the file locally - I honestly do not recall where on the WWW I found it, but have had it for a bit, and thought it worth sharing).

Everyone else: This is just a rudimentary guide to sterilization techniques, lab techniques, pure culture procedures, etc. As the title states, it is predominantly for diagnosing fungi/bacteria which insects harbour, but I think the advice in there is sound for the types of work which we are engaged in....Hope someone finds it helpful.

Since I do not recall where I DL'd it from, I posted it here for people to snag:

http://www.charm.net/~sj/lab_techniques.pdf

HTH,


~YSJ~


--------------------
"There are no angels unless they are Angels of Death...And I would never again doubt my place among them or lose my resolve to serve in their wild ranks."         
                                                            -T. Ligotti


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OfflineWildCardsRevenge
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Re: Something which is elementary, but which may prove useful... [Re: YSJ] * 1
    #1457215 - 04/14/03 03:59 PM (14 years, 15 days ago)

Great find there, Hopefully everyone takes a look at this and can learn something to help them out.


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OfflineMAIA
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Re: Something which is elementary, but which may prove useful... [Re: WildCardsRevenge]
    #1457573 - 04/14/03 05:52 PM (14 years, 15 days ago)

Everyone should read stuff like that. Thanks for the link !

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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OfflineOddish
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Re: Something which is elementary, but which may prove useful... [Re: YSJ]
    #1457582 - 04/14/03 05:59 PM (14 years, 15 days ago)

Thanks a lot! Definitely a good read.


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OfflineWildCardsRevenge
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Re: Something which is elementary, but which may prove useful... [Re: Oddish]
    #1457663 - 04/14/03 06:24 PM (14 years, 15 days ago)

I'm making this sticky so everyone takes a look at it


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OfflineYSJ
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Registered: 01/31/03
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Re: Something which is elementary, but which may prove usefu [Re: WildCardsRevenge]
    #1458160 - 04/14/03 09:32 PM (14 years, 14 days ago)

Yeah, I was kinda sold on its usefulness when I saw the following in all caps:

THERE IS NO SUCH THING AS MOSTLY STERILE

and

YOU ARE ONE BIG CONTAMINANT

and

SHORT CUTS = CONTAMINATION

Kinda sums it all up, really ;D



~YSJ~


--------------------
"There are no angels unless they are Angels of Death...And I would never again doubt my place among them or lose my resolve to serve in their wild ranks."         
                                                            -T. Ligotti


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OfflineYSJ
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Re: Something which is elementary, but which may prove usefu [Re: MAIA]
    #1459164 - 04/15/03 03:02 AM (14 years, 14 days ago)

Glad it was useful, and not a prob :smile:

Now, if we could just make up a simple listing of the UNHOLY HORRORS which imbibing of contaminated fruits could bring upon one....That, along with reasons why NOT TO PHUCK AROUND WITH CLEARLY CONTAMINATED JARS.  Sheesh.

I keep seeing posts from ppl saying things like "I sniffed this bio-bomb I made and man, was I sick as hell..."

Ok.  Not trying to rant here - and besides, it isn't me, it's uh....The booze.  Yeah, that's it :wink: - but seriously, folks.  Use some common sense. 

I was wearing an N-95 breather when I got the scraping to make the slides with that green devil (post below, vide infra).  I don't get a huge number of contams (my ratio is actually still pretty damned good, believe it or not, and virtually all vectors have been established, much to my delight), but still, when I *DO* get one, I treat it with respect.

Barring posting a veritable laundry list of evils which can be inflicted upon oneself from messing around w/contams which is so horrifying as to turn the amateur mycologist away from the hobby entirely, is there anything ELSE we can do to instill a proper amount of respect (fear...?) into people about just how nasty contams can be?

Anyone?  Any suggestions?

I have a lot of textual stuff on the nasty shite which various opportunistic fungi/bacteria can do, as well as some lit regarding simply receiving horse-doses of shite that wouldn't normally harm those who are not immunocompromised (e.g., the CDC BL handbook addendums lists a few cases of ordinary researchers that got fux0red by things not normally infectious or hazardous to humans, but which receiving a whopping dose of was enough to deleteriously affect their health...) - and, while not trying to be a wet rag, I'm tempted to post a good helping of that sort of info here, just to scare some sense into people.

Please note that I'm not bashing anyone who's ever got sick off a contam - I mean, hell!  A mere N-95 was CLEARLY not enough protection against the nasty I was viewing under the 'scope....Those spores are still almost too small to see @ 500X, which would mean they'd probably slip thru an N-95 with little difficulty, which means *I* could fall ill...Contrariwise, I *did* get that sample knowing full well the gamut of risks I faced, so have no one to blame but myself...

Cheers,


~YSJ~ 


--------------------
"There are no angels unless they are Angels of Death...And I would never again doubt my place among them or lose my resolve to serve in their wild ranks."         
                                                            -T. Ligotti


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Offlinepsilo9com
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Registered: 05/20/03
Posts: 267
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Re: Something which is elementary, but which may prove usefu [Re: YSJ]
    #1579800 - 05/26/03 11:49 PM (13 years, 10 months ago)

YOU ARE ONE BIG CONTAMINANT.


When you think about it. Life is just a bunch of contaminants eating eachother.

I like mine fried


Edited by psilo9com (05/26/03 11:52 PM)


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OfflineTheHobbit
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Re: Something which is elementary, but which may prove usefu [Re: psilo9com]
    #1724618 - 07/17/03 01:44 PM (13 years, 9 months ago)

I guess everyone's home/environment is a bit different, some being more prone to contamination than others of various sorts, but I wonder how often we all experience contamination. Even though my own experience is limited (newbie +), just taking basic precautions such as cleaning the innoculation area reasonably well in advance of doing so, busting out the Lysol, taking a shower beforehand, using a mask and rubber gloves, and using the Oven Tek, I've had minimal contams, like 2 jars out of maybe twenty, not too bad of a ratio, especially for a newb - and that's with dogs and cats everywhere in the house.
How do others fare with contams? Think ten-percent is not too bad?


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Offlinecasper702
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Registered: 07/06/03
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Re: Something which is elementary, but which may prove usefu [Re: TheHobbit]
    #1924723 - 09/17/03 12:03 PM (13 years, 7 months ago)

Read it, shit I pinted it and thanks for the link, this should be posted in the FAQ's or something


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OfflineJackolantern
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Re: Something which is elementary, but which may prove usefu [Re: casper702]
    #1959208 - 09/28/03 09:41 AM (13 years, 6 months ago)

That information just saved me about 5 minutes of extra work. Thanks guys... I'll think about the shroomery if my birthing is uncontaminated.


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Offlinecatalyst777
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Re: Something which is elementary, but which may prove useful... [Re: YSJ]
    #2020862 - 10/18/03 05:43 PM (13 years, 6 months ago)

Ha! I remember 7 years ago, when I did my first PF jars. Absolutely no contams at all! And amazing yields.

The thing is, I took no precautions at all. No lysol, glove box, mask or anything. Strange how I've had problems since then AFTER taking precautions. I think I just had really good karma the first time :smirk:

Still, I rarely have a problem.

Good luck all... 


--------------------
Facts do not cease to exist just because they are ignored.

Aldous Huxley


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Offlinepietruk
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Re: Something which is elementary, but which may prove useful... [Re: YSJ]
    #2339849 - 02/16/04 03:28 AM (13 years, 2 months ago)

Great link. Hope everyone takes a look at it.


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Invisiblepsilomonkey
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Registered: 08/08/03
Posts: 812
Loc: Airstrip One
Re: Something which is elementary, but which may prove useful... [Re: YSJ]
    #2840371 - 06/29/04 05:14 PM (12 years, 9 months ago)

Nice find. Good advice, well written a rare thing indeed.


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Offlinechemcrusader
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Re: Something which is elementary, but which may prove usefu [Re: YSJ]
    #2882749 - 07/12/04 05:13 PM (12 years, 9 months ago)

Thanks a lot. Down with contams!


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OfflineraSman
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Re: Something which is elementary, but which may prove usefu [Re: YSJ]
    #2901867 - 07/18/04 09:50 AM (12 years, 9 months ago)

nice find


--------------------
Free Chain Of IPods
This is quite possibly the easiest way to get something free on the internet. When asked which offer(yea, just 1) you will complete, you want to choose video professor. You can get a full refund on shipping and everything. Once five people (don't even have to know them) complete the offer as well...you get an IPod. I sh*t you not.


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Offlinecaimen
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Re: Something which is elementary, but which may prove usefu [Re: raSman]
    #2902268 - 07/18/04 01:53 PM (12 years, 9 months ago)

All i can say is kick ass


--------------------
MAN Made Speed
GOD Made Weed
<Who do YOU TRUST ? >

http://www.shroomery.org/index/par/25954


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Invisiblefastfred
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Re: Something which is elementary, but which may prove usefu [Re: YSJ]
    #3078694 - 09/01/04 06:31 PM (12 years, 7 months ago)

Quote:

YSJ said:
Now, if we could just make up a simple listing of the UNHOLY HORRORS which imbibing of contaminated fruits could bring upon one....That, along with reasons why NOT TO PHUCK AROUND WITH CLEARLY CONTAMINATED JARS. Sheesh.




Maybe we should wait for someone to actually get sick first, before we start telling everyone how sick it will make you.

Has there ever been a documented case?


-FF


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Offlinepacopaco
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Re: Something which is elementary, but which may p [Re: YSJ]
    #3219437 - 10/05/04 07:53 PM (12 years, 6 months ago)

do you wanna be the first? That's like being the drunk guy at the party that says "Hey look, i can juggle hot coals"....


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OfflineIGnosticAbhorI
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Re: Something which is elementary, but which may prove usefu [Re: pacopaco]
    #3329294 - 11/07/04 01:57 AM (12 years, 5 months ago)

I only wish I had some of those things, very professional.Thanks for the .pdf and gl


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Amazon Shop for: ½ Pint Jars

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