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Offlinebik123
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Registered: 07/31/10
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Impulse sealer
    #14566930 - 06/05/11 10:25 PM (12 years, 7 months ago)

After autoclaving the grain spawn in the bags and letting it cool down in the lab, i noticed water condensate inside the plastic bags.
And after transferring the grain spawn, (grain-grain) i used the impulse sealer but it did not sealed it, due to the condensate.

Any idea what to do?


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OfflinePitcherCrab
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Re: Impulse sealer [Re: bik123]
    #14567603 - 06/06/11 12:37 AM (12 years, 7 months ago)

Wipe out the bags in a clean environment and try to seal them again? Or you could skip the sealer altogether and use a zip-tie instead. Then you wouldn't have to worry about the cleanliness of whatever it is you're using to wipe out the bag with.


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Offlinefungus_tao
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Re: Impulse sealer [Re: PitcherCrab]
    #14568025 - 06/06/11 04:34 AM (12 years, 7 months ago)

Adjust the controller on the side of your sealer until you get a good seal that won't break when you shake the bag.


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Offlinebik123
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Re: Impulse sealer [Re: fungus_tao]
    #14568262 - 06/06/11 06:50 AM (12 years, 7 months ago)

Or, in the clean to semi-clean lab, after autoclaving, would it be good to just open the bag and let the hot air escape. And then the condensate will escape as well and sealing will not be a problem.

But will contamination set in as a result as it cools for more than 10hrs?


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Offlinefungus_tao
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Re: Impulse sealer [Re: bik123]
    #14569707 - 06/06/11 02:33 PM (12 years, 7 months ago)

I actually seal bags before autoclaving...I know... but I've never had a bust bag except for when I was using a PC and it ran dry. Also, you can make three 1" folds in the top of the bag and use some clamps to keep the top of the bag folded shut. I've kept sterilized supp. sawdust on the shelf in open air for 3 months+ before inoculating that were NOT SEALED using this method. I used paper clamps available at office supply stores like these.


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