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InvisibleAleon
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My Low Cost Mini-Lab/Flow Hood Setup
    #14564817 - 06/05/11 02:10 PM (12 years, 7 months ago)

Here is my mini lab setup.  Ive been using this same setup (at 2 different locations) for the past 1.5 years.  I was very low on cash and i had and extra inline fan that wasnt in use, so i put this macgyvered flow hood together.  I haven't had contamination since i built this flow hood.  I Used a 4" inline fan with a carbon filter(available at all hydroponic stores), 4" ducting, a small plastic tote, and cheap hepa filter replacements for fans (from walmart).  I already had the fan so that was $0, the tote was like $5, the 2 hepa 4"x10" were $30, and the carbon filter was $70 , the plastic shelving unit and 4mil B+W plastic was about $50 total; for a total of: $155. I wanted to put this out there for people who have a little money but not enough for a professional flow hood.  Dont forget to have a cleaned rubber tire for breaking up the spawn in the jars; it works wonders and prevents work-related injuries.









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Invisiblemister
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: Aleon]
    #14564847 - 06/05/11 02:19 PM (12 years, 7 months ago)

Good work. simple and to the point.


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:mushroomgrow::mushroomgrow::mushroomgrow::mushroomgrow:AMU Q & A thread:mushroomgrow::mushroomgrow::mushroomgrow::mushroomgrow:


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Offlinecurry
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: mister]
    #14565077 - 06/05/11 03:13 PM (12 years, 7 months ago)

Kudos to you, very impressive low-cost design.  I'm still in a glove box, so if I ever decide to upgrade, I think I'd definitely go with a design like that.  Thanks for sharin'.


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Invisibleloucal
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: curry]
    #14565830 - 06/05/11 06:28 PM (12 years, 7 months ago)

I dunno curry, I think once you factor in the price of the inline fan and carbon filter you are paying what you would pay for the parts to a legit laminar flow hood.  The carbon filters are for filtering out smell, I don't know what kind of microns that is effective to, but it must help a bit since I know those hepa filters he has there are about as effective at filtering out contams as a window screen is at keeping out tiny gnats.  Not tryin to hate, I just think the $155 could be appropriated better and the carbon filter and inline fan are overpriced parts for what they are being used for.  Like I said, not hating just saying if you are buying all your parts build a real laminar flow hood, or throw the cash in the bank for a rainy day and use a glove box.  Those two have scientific basis for allowing clean culturing, these types of setups have a few posters boasting 'no contams' but no real numbers (no contam at all isn't even realistic in a clean room with a laminar hood over time).  I'm going to personally go the laminar route when I get fed up pouring hundreds of plates and inoculating slants in a glovebox.  Grain to grain will be much nicer too :smile:


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InvisibleAleon
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: loucal]
    #14566281 - 06/05/11 08:13 PM (12 years, 7 months ago)

LOL, My claims to have no contams!  I wonder how im running a oyster mushrooms farm for a living if i was having contams.  Not trying to be rude, but from you pictures it looks like you have great success with your setup(not).  Keep it to yourself if you cant even grow uncontaminated cultures.  Plus, i already had pretty much everything left over from all my hydroponics growing(i have tons as i grow hydro and aquaponic herbs.salad greens to sell to frmrs markets, coops, and restraunts.) If you want to toss me the $ to get a real flow hood let me know and ill give you my adress so you can drop me a check.  There was no need for you to post as you did Loucal, you didnt add anything useful to the thread. And like i said i havent had contams ever since i built it.


--------------------
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www.swordandshieldwellness.com


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InvisibleNot Quite Social
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: Aleon]
    #14566604 - 06/05/11 09:25 PM (12 years, 7 months ago)

Aleon, you said you sell at farmers markets.  I got a question for you.  Do you sell at different markets on different days, or do you have vendors at different markets selling for you, and, if the latter is the case, what's their cut/charge?  Thanks.


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InvisibleAleon
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: Not Quite Social]
    #14568360 - 06/06/11 07:55 AM (12 years, 7 months ago)

I never have other people sell my work at farmers markets, ever!  I run this business with my life partner, and would never put our work in the hands of some middle-man market vendor who doesnt know shit about growing fungi.  I want people to buy from the cultivator so they can ask questions and become informed.  Please dont farm out your work, its degrading the family farms image.  Also, by not getting other people to sell my produce at frmrs mrkts, i leave open more niches for other micro-mushroom farms to open instead of monopolizing (this isnt about $ if you couldnt tell); this is why i dont hide any of my techniques to the public.  I cannot meet the demand, so why not let some1 else enjoy a life where they can grow mushrooms for a living too. :headbanger:  We do markets every saturday and sunday.  Between that, coops, and restaurants there is no need to go to more markets.  We sell oysters for $10/lb ($7/lb wholesale)and no one has ever complained about that price. The quality is unmatched, and they are supporting a tiny family run company, not some suit and tie business fags looking to make a quick buck off this whole "green/organic" trend.


--------------------
Mushroom medicines available at:
www.swordandshieldwellness.com


Edited by Aleon (06/06/11 08:04 AM)


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Invisibleloucal
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: Not Quite Social]
    #14568385 - 06/06/11 08:04 AM (12 years, 7 months ago)

Hey man, if you want to take offense that's your choice, but you will undoubtedly eventually realize that all you are doing is blowing non-sterile air all over your plates.  Those filters state the efficiency right on them, its usually in the way low 90%s when flow hood teks clearly state you even want the 99.99% over the 99.97% filters.  I'm just saying you don't have laminar flow and trying to help someone else who might spend the same amount that a flow hood costs to 'macgyver' up a setup that may work for your purposes, but is still blowing contams all over your work.  Oysters grow really fast and almost always overrun contams even if they get a chance to start.  If you don't think you have contams on your dishes then you must fundamentally disagree with Stamets.

You have the tire for breaking up grain spawn (which I think RR has even outright said at one point will always be dirty) right in front of the intake where the carbon filter is!!

As for the one plate I have up here of CM, first of all you are comparing apples to oranges.  That was a transfer from a plate I traded for that came from someone elses lab and went through the mail!  Plus I didn't even ever claim to have 'no contams' even though I rarely do and I pour hundreds of plates/slants a month.  FYI though the section I thought might be a contam on that plate actually did grow together with the CM with no visible zone of inhibition and I looked at it often.  I am still experimenting with cordyceps and if you know something about it I do encourage you to post in the thread.

Like I said, just trying to help out someone who does NOT have that stuff lying around before they waste their money, by pointing out that it is NOT laminar flow.  You did call it a flow hood, which could technically be ok but it is misleading because when texts mention using a flow hood they are always talking about a laminar flow hood.  The mods will correct me if I'm wrong here, but I think in this case they might be in agreement.  A carbon filter as it is here is just an overly expensive prefilter.


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InvisibleAleon
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: loucal]
    #14568405 - 06/06/11 08:22 AM (12 years, 7 months ago)

Honestly i feel that you dont know what your talking about.  I never said this is the best way to go, i just outlined my setup; thats it.  I had a choice with the little money i have, to automate my fruting chamber or get a professional laminar flow hood.  I chose to automate my fruitng chamber so i dont have to hand water 2-3 times a day. Why would i fix something that aint broken.  And plus i do not just grow oysters, ive done shitake, lions maine, maitake, morel(spawn), and others; so this setup works for more than just oysters.  And your claims about the carbon filter just removing smell is BS.  They do much more than that; many hospitals use carbon filters installed throughout the air system to remove germs from the air that are created by sick patients, so sickness isnt transfered.  Also, who named you the flow hood police???  Leave me alone.  I didnt tell anyone to build this; i laid out my price and told the truth.  I never get contamination.  About the tire thing, it can be cleaned buy it new and submerge it in bleach.  You are hilarious, you are limiting reality to figures you read in books & on forums, not first hand experience.  Build what i have and try it for yourself, then you can chime in.  I dont wnat to hear what you think would and wouldnt work, i want to hear what you've tried that does or doesnt work. If you have $ for the pro laminar i would suggest that over my setup.  I just didnt have the money (way to rub it in J-Ass) This works for me, OK.  To each his own!


--------------------
Mushroom medicines available at:
www.swordandshieldwellness.com


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Invisibleloucal
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: Aleon]
    #14568503 - 06/06/11 09:01 AM (12 years, 7 months ago)

I'm sorry buddy, I didn't realize 41 posts on shroomery made you an expert on not only everything that has to do with practically growing mushrooms, but also on MY personal experience :rolleyes:

I am going to leave you alone, mostly because this thread depresses me.  I am a big fan of local food but this is an example of why big corps and politicians are always attacking the little guy.  You sell food to people for a living and you are totally skimping on your sterile technique.  You sir, are the hilarious one.  I don't see RR with a jerry rigged flow hood, he produces too much for it to be possible.  Make sure you have enough money to not get people sick before you start trying to make your own spawn, you can always buy it from the reputable vendors.  This is one thing that those 'fags' you speak of in suit and ties do correctly because they know when to pass the buck.

It has been said 1000 times and clearly you just don't have enough experience under your belt to have had a petri dish multiplied into thousands of pounds of substrate, but trust me if you tried to do that with your setup it would be a disaster.

I don't think you know what you are talking about, attacking me when I am giving straight facts, learn to take some constructive criticism and you might learn something.  If you came in here saying you even tried to 'dial it in' and showed a bic lighter test with this 'flow hood' then I would still be shaking my head but at least you would have demonstrated that you did a little bit of research before you jumped into this.  So to summarize, no I will not be building one of these to test it out, you spent $155 on all your supplies minus a fan.  All a flow hood needs is a prefilter, fan, the wood and hardware for the box and a dimmer switch.

http://www.filtera-b2b.com/businessfilters/hepa.htm#99.99wood
18 x 18 x 5-7/8 (99.99% HEPA wood) $117.31

That's an 18 by 18 filter, if you spent your money on that and wood you would have a laminar flow hood for the same price and you would be killing it compared to what you have now, that just appears clean to the naked eye. I have never built one with an inline fan, but I think it would still work if you worked out the numbers and had the right size. Your welcome for dropping the knowledge and the nudge you need to do it right the next time, now quit sniffling and get your last word in, because I'n not worried about helping you anymore and I have provided enough information to help anyone else to more easily not make the same mistake.  Like you said, to each his own, but your not going to tell me not to chime in until I have built something half ass for the same price range that I and others have constructed laminar flow hoods.


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InvisibleEvilMushroom666
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: loucal]
    #14568582 - 06/06/11 09:27 AM (12 years, 7 months ago)

Quote:

loucal said:
learn to take some constructive criticism and you might learn something.




I saw nothing wrong with lou`s original post, he was stating he was not
trying to hate on you, but was simply giving you information on another way
to go about things. Clearly what you have set up works for you, but there
is ALWAYS room for improvement in everyone`s personal setup, that even
includes RR and other commercial cultivators.

What works for you, may not work for others as I have learned in this
hobby. Learn to take some constructive criticism and IMO you will be
a lot better off my friend. We are all on the same side, trying to
accomplish the same goals, no need to be so defensive my friend.


Edited by EvilMushroom666 (06/06/11 09:37 AM)


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InvisibleAleon
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: EvilMushroom666]
    #14568991 - 06/06/11 11:31 AM (12 years, 7 months ago)

I apologize for the harshness, i have been getting alot of people hating on me l8ly.  I understand he was stating his opinion, but so was i. 

"Those two have scientific basis for allowing clean culturing, these types of setups have a few posters boasting 'no contams' but no real numbers (no contam at all isn't even realistic in a clean room with a laminar hood over time)" 

I think that this is what upset me (especially the boasting part).  B/c im wasnt trying to look cool, or be a lier; i was and am telling the truth.  I really havnt had any contams (when using clean cultures; i have had some trichorderma when cloning a strains i find in  the woods, but very little) And ive been using this same setup for 1.5 years.  And i never said this was the best; its actually just enough to squeak by.  I only posted this b/c i felt i was asking to many questions on this forum, and i wanted to share something.  Obviously, i am still learning and shouldnt care about sharing my experiences b/c they are not using the top-of-the-line equipment.  People grow mushrooms in 3rd worlds countries by the masses with no electricity or sterile technique; i just find it hard to beleive that you must have "the best" equipment to succeed (though the best equipment will improve things).

Also a question about "Make sure you have enough money to not get people sick before you start trying to make your own spawn".  I am wondering, if there is no visible contaminants can there still be a health risk?  As you said even real laminar flow hoods get some contams so if you cant visually c them, how could even the people with the pro laminar flow hoods not be a risk of poisoning the end consumers?  I honestly want to know this for the health of my customers.  I only c mycleium when i grow things out, if i see a contam (which is extremely rare)i throw it out; i dont expand it.

Once again i apologize, life has been frustrating these past few weeks. (on a side note, normally the most heated up conversations generate some of the best thoughts/ideas;  destruction is a form of creation.)


--------------------
Mushroom medicines available at:
www.swordandshieldwellness.com


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OfflineRogerRabbitM
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: EvilMushroom666]
    #14569019 - 06/06/11 11:36 AM (12 years, 7 months ago)

Let's all shitcan the hostility please.  It serves no purpose.

Also, for my time and money, it's far better to have someone else go to the farmer's markets and add my mushrooms to their own product mix.  Why?  Because if I'm at a market, I'm not here growing mushrooms.  In addition, perhaps my situation is unique in that I live in the wilderness and the closest market is nearly an hour drive to a town with only 600 for total population.  The rest of the markets up here are two to three hours away.  That means a full tank of gas, and it's awfully hard to make ends meet when the $5/gallon gas(yes $5, that's not a typo) eats all my income, and while I'm there, I'm also not building and sterilizing substrates that day. 

If I were to go to the markets myself, I'd be providing essentially free food for people while we go hungry.  It's also extremely inefficient and a waste of gas and time for each vendor to take a separate vehicle to the markets when one vendor can handle the product for several farmers.  It's the only way to make it work in this area, and we're busy setting up a local farmers co-op to improve efficiency even more.  The gentleman who sells my mushrooms at the farmers markets is 78 years old, and even at his age, he comes here to work for me one day a week.  This way, he knows the whole process and can talk mushrooms to the customers just as easily as I can.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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InvisibleAleon
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: RogerRabbit]
    #14569657 - 06/06/11 02:18 PM (12 years, 7 months ago)

It seems you have a unique situation, living where you do. Correct me if im wrong; you really love growing the mushrooms! :cool:  I think that there are different needs/wants for each mushroom cultivator.  You love to grow mushrooms, i love to interact with the customers and grow mushrooms There must be balance for what i want to work, so obviously i wouldnt be able to grow as mush mushy as some1 in your scenario); just different preferences. But my point is (IMO) that since i dont have to spend as much on gas because of my close proximity to markets or give a cut to the vendor, i dont need to grow as much to make the same amount of money as some1 who grows more mushrooms (due to reduced delivery and middleman costs). Many times i run multiple errands when i deliver mushys so that i make the best use of my gas. Also i bought my property because of its close proximity to markets, thereby reducing the "carbon footprint" as people call it today.  Even though one can give mushys to a vendor, he/she still must drive to get it; with gas prices rising (holy shit its $5 where you are, lol) i think the need to locate the food production facilities as close to the end consumers as possible is very important. Even the market vendors may not be able to afford the gas to pick up and deliver the produce if current gas price trends continue.

I apologize for the hostility again, ive had a rough week (though thats no excuse).


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Mushroom medicines available at:
www.swordandshieldwellness.com


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Invisiblenboy
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: Aleon]
    #16652884 - 08/06/12 10:59 AM (11 years, 5 months ago)

Way too late on this one but what do you have against 'fags'? Are you homophobic or just wanting to sound cool or ignorant? You don't sound cool you sound like an idiot.  What kind of professional gets in these kinds of arguments and uses phrases like 'fags' and 'J-Ass' Sorry man, your words are bogus.


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InvisibleAleon
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: nboy]
    #16653069 - 08/06/12 11:41 AM (11 years, 5 months ago)

My words are bogus. Dont listen to them, ever.


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InvisibleJavadog
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: nboy]
    #16655968 - 08/06/12 07:19 PM (11 years, 5 months ago)

Quote:

nboy said:
Way too late on this one but what do you have against 'fags'? Are you homophobic or just wanting to sound cool or ignorant? You don't sound cool you sound like an idiot.  What kind of professional gets in these kinds of arguments and uses phrases like 'fags' and 'J-Ass' Sorry man, your words are bogus.




Hmmmm...I searched the page and did not see the use of this word.

Aleon is cool.  ...had a bad week a year ago maybe...

JD


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Invisiblenboy
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: Aleon]
    #16657815 - 08/06/12 11:22 PM (11 years, 5 months ago)

I'm just trying to build flow hood.Don't get me wrong. I've never read any of your other posts 
Quote:

Aleon said:
The quality is unmatched, and they are supporting a tiny family run company, not some suit and tie business fags looking to make a quick buck off this whole "green/organic" trend.


I just don't think that kind of language should be tolerated or accepted.

I'm sure you've got plenty of wisdom and great mushroom growing skills. But honestly, I would never buy mushrooms or really anything from someone that spoke like that publicly in talking about their business.


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Offlinecloudpersona
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: nboy]
    #16659064 - 08/07/12 07:21 AM (11 years, 5 months ago)

Quote:

nboy said:
I'm just trying to build flow hood.Don't get me wrong. I've never read any of your other posts 
Quote:

Aleon said:
The quality is unmatched, and they are supporting a tiny family run company, not some suit and tie business fags looking to make a quick buck off this whole "green/organic" trend.


I just don't think that kind of language should be tolerated or accepted.

I'm sure you've got plenty of wisdom and great mushroom growing skills. But honestly, I would never buy mushrooms or really anything from someone that spoke like that publicly in talking about their business.



Leave it alone, shit's a year old.


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
– Terence McKenna
“If the words ‘life, liberty, and the pursuit of happiness’ don’t include the right to experiment with your own consciousness, then the Declaration of Independence isn’t worth the hemp it was written on.”
-Terence McKenna


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Offlineegodeathflux
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: cloudpersona]
    #16659134 - 08/07/12 07:44 AM (11 years, 5 months ago)

:horrifying:


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"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




"Standing on the corner of 5th and Vermouth"



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InvisibleAleon
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: egodeathflux]
    #16659701 - 08/07/12 10:30 AM (11 years, 5 months ago)

Do people not make mistake?  Is any human perfect?  I apologized and there is no more i can do.  I feel different than the person i was yesterday, not to mention a year ago.  Im in my young 20's and grew up in a materialistic rich suburb of chicago where i was programed to be someone who i am not.  Everyday i try to dismantle my disguise, which i didnt even now existed until recently.   

"The passage sums up Confucius's own spiritual and intellectual milestones: “The Master says: At 15, I set my heart on learning. At 30 I know where I stand (my character has been formed). At 40, I have no more doubts, at 50, I know the will of Heaven, at 60 my ears are attuned (i.e. my moral sense is well-developed), at 70, I follow my heart’s desire without crossing the line (without breaking moral principles).” "

I am still an unopened blossom just learning (pain, suffering, and mistakes are some of the best tools for development in the learners toolkit).  Most sage's (confucius, Lao Tze, etc) and wise men seem like assholes to the public (they would pick me apart!) because they say it how it is. Which is... that we all contain within us the answers to all of our problems by realizing we all come from one infinite.  And bad has to exists for good to exists; the yin and yang.


Edited by Aleon (08/07/12 10:38 AM)


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InvisibleMykes logos
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: Aleon]
    #16661955 - 08/07/12 05:32 PM (11 years, 5 months ago)

Really efficient setup OP!

I just invested about $2,500 into my lab and laminar flowhood build, which has taken weeks of planning, building, and waiting, etc... and now I'm seeing that you're doing all that with that? wow!

If you're supporting your family with that, you obviously know what you're doing. It may look "sketchy", if you please, but wow- I'm mad impressed!

It takes a skilled cultivator to do what you do with that set-up:congrats:


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InvisibleAleon
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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: Mykes logos]
    #16663243 - 08/07/12 08:48 PM (11 years, 5 months ago)

I got rid of that setup the day i posted it.  A laminar flow hood and state-of-the-art clean room technology is the preferred route, if possible.  If not use what you can to make it work FOR YOU! At the time this was all i had to work worth, But as stated earlier, for over a year i used this setup in 2 different locations and was able to make enough clean culture/spawn in it to run a small sized oyster on past. straw farm.  I would past. the straw in a 55gal drum with propane burner underneath and could do 4 bathes a day.  I grow on supp. sterilized sawdust now.  The yield/ft3 is absurdly higher using a sub as dense as sawdust on shelves; compared to cereal straws packed into 6' columns or 5 gal buckets stacked 5 high.

I have little mush cultivation skill (only been growing mush for 4 years), mostly just luck.  And i try to repay that luck by learning how to replicate it, then share that data. I am a skilled observer in this area, which IMO is the most important, due to the amount of plants i have grown indoors using CEA cultivation (which is the same as CEA mushroom farming just a different organism).  This is how i got my "baseline" for my current mushrooms farm production procedures; trial and error, luck, w/e you want to call it. :shrug: 

Good luck.


--------------------
Mushroom medicines available at:
www.swordandshieldwellness.com


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Re: My Low Cost Mini-Lab/Flow Hood Setup [Re: Aleon]
    #16664994 - 08/08/12 08:32 AM (11 years, 5 months ago)

Yes Aleon, I've been looking through some "lab build/cleanroom" threads as of late, because I just built my own and am about to build a laminar flowhood as well- actually just ordered my HEPA from Fungiperfecti yesterday...

I saw these here- I am very impressed:








Well done. I've noticed as I've gone from being curious to getting into mushrooms hunting to reading and learning about cultivation... and am now well beyond the "pondering" stage, that most of this hobby appears to be "home-grown" in a sense. I'm pretty sure all the top cultivators in the country, and even some on here who run their own farm that they started from scratch, started with Stamets books and pf-teks, etc.... It's more "self-taught"- which is the fun part!

It's really cool seeing the "evolution", if you please, of your lab set up over the past couple years- all at the click of a button.

Thanks for your contribution to this community- it helps beginners like me out so much!

:cheers:


Edited by Mykes logos (12/20/12 08:52 AM)


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