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Offlineneuron99
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Registered: 04/02/11
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Last seen: 5 years, 9 months
~=.Neuron's grow. [w/ PIX!]=~ * 1
    #14564722 - 06/05/11 01:39 PM (12 years, 7 months ago)

Hello everybody! Coming from the world of cannabis, this is my first time growing psilocybin.

I've lurked these forums and have already soaked up so much information, it's exciting to embark on this journey with the help of such a knowledgeably community.



I ordered spores but forgot which strains I chose... I do know the order code which was the following:

> eq2: 1
>
> Lipa1: 1
>
> Tx2: 1

so whatever those may be (if anyone can recognize the shorthand plz let me know!), I'm just excited to have spores and begin!

special thanks to member "realfuzzhead" for lending a helping hand!


here's the game plan--not that you guys need to know--but more for myself, as I keep forgetting the stages and specifics lol


1.) purchase cubensis, WBS, pressure cooker, latex gloves, mason jars, silicone, tyvek , 99% isopropyl alcohol and power drill
2.) prepare liquid culture, prepare WBS
3.) inject WBS with LC, wait and let jars colonize
4.) spawn WBS to pasturized coir, let it colonize
5.) place tray in fruiting chamber
6.) harvest and dry


I've decided on the following teks:

-Cyber's LC Tek (opting to use honey)
-Cyber's self-healing lids
-6T WBS Tek
-Damion5050's Coir Tek
-Monotub





My first trip to Walmart, I need to go back b/c the pressure cooker is missing a part :frown:  Clear tub is to build a glovebox for myself, I still need to go get some PVC couplings and glue.





Since I have to go back to Walmart I decided might as well verify with you guys if I got the suitable items


I want to verify that this is the best type of polyfill.... I noticed many kinds, including higher density premium version as well as quilted versions




Also, this is the WBS I have chosen:




This was the only honey at Walmart, will this work?  There are organic stores nearby (whole foods/bristol farms) that I can go to if it really makes a difference, I do want to do everything right the first time.

Also, is the proper RTV? I noticed some posts ppl were using the stronger grey color:








Thanks!!!!


Edited by neuron99 (09/05/11 01:06 PM)


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InvisibleDoubleG909
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Posts: 136
Re: Neuron's first grow :) [Re: neuron99]
    #14564755 - 06/05/11 01:51 PM (12 years, 7 months ago)

Can you return your blue rtv and get the red tube? The red is high-temp and will withstand the PC. I see you have polyfill and wbs so I'm guessing your jumping right into bulk with a monotub? If so you may wanna get some quart sized mason jars. I like that your making a glove box right from the get go!


--------------------
“Nature is not our enemy, to be raped and conquered. Nature is ourselves, to be cherished and explored.” ― Terence McKenna



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Offlineneuron99
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Re: Neuron's first grow :) [Re: DoubleG909]
    #14564775 - 06/05/11 01:59 PM (12 years, 7 months ago)

Quote:

DoubleG909 said:
Can you return your blue rtv and get the red tube? The red is high-temp and will withstand the PC. I see you have polyfill and wbs so I'm guessing your jumping right into bulk with a monotub? If so you may wanna get some quart sized mason jars. I like that your making a glove box right from the get go!




I was looking specifically for the red type but unfortunately they didn't have any.  The temperature range for the blue was -65*F to 500*F, the other grey one IIRC had the same temp range but had less flexibility.  I was thinking more flexibility would translate into more "pokes" before I had to re-silicone the injection port

I am however going to a different Walmart today so I'm bringing the RTV with me and snagging the highest temp gasket-maker silicone

Yeah the pint jars are for the honey LC, I will be purchasing quart jars for the bulk :smile:


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InvisibleDoubleG909
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Posts: 136
Re: Neuron's first grow :) [Re: DoubleG909]
    #14564776 - 06/05/11 02:00 PM (12 years, 7 months ago)

If you don't want to wait for your LC to fully colonize you can do your first batch of jars multispore. Use like 1 or 2 cc's from the syringe into your LC and the rest on your WBS.


--------------------
“Nature is not our enemy, to be raped and conquered. Nature is ourselves, to be cherished and explored.” ― Terence McKenna



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Offlineneuron99
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Re: Neuron's first grow :) [Re: DoubleG909]
    #14564798 - 06/05/11 02:06 PM (12 years, 7 months ago)

Oh that's a good idea I didn't think of that

Maybe I'll use 6cc of the 10cc of each strain, while reserving 4cc to make LC (enough for two attempts @2cc ea. in case the first one fails to colonize)

So using 6cc, that's 3 quart jars? Gotta start crunching numbers to figure out which size monotub to get


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Offlineneuron99
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Re: Neuron's first grow :) [Re: neuron99]
    #14566705 - 06/05/11 09:43 PM (12 years, 7 months ago)

Okay back from Walmart and Home Depot with a fresh new pressure cooker and some new goodies! :smile:


Quart jars for WBS
Plastic wide-mouth lids
Red RTV Silicone
Toilet flanges for glovebox
Hoseclamps to secure gloves
Drill bit kit
Epoxy
(Not pictured: stack of USPS Express mail Tyvek)




Edited by neuron99 (06/05/11 10:16 PM)


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Offlinek00laid
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Re: Neuron's first grow :) [Re: neuron99]
    #14566718 - 06/05/11 09:46 PM (12 years, 7 months ago)

:shrug: i dont even use rtv silicone.

i use the bathroom stuff.

PCs just fine :thumbup:


--------------------
AMU - AMU Q & A - MyVideo Teks!


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OfflineShroom_i
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Re: Neuron's first grow :) [Re: k00laid]
    #14566777 - 06/05/11 09:59 PM (12 years, 7 months ago)

Lookin good to me, I'll be following this.


--------------------
Prisoner#1 said:
[quote]destructo_low said:
Prisoner's posts by percentage.

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[/quote]

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Invisiblehiggledy-piggledy
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Re: Neuron's first grow :) [Re: Shroom_i]
    #14567055 - 06/05/11 10:45 PM (12 years, 7 months ago)

:congrats:

you are prepared.

(one of the very few people)


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InvisibleGzaajhom
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Loc: USA
Re: Neuron's first grow :) [Re: Shroom_i]
    #14567059 - 06/05/11 10:46 PM (12 years, 7 months ago)

Looks good. I would suggest making a myc syringe instead of an LC though. Basically, what you do is inoculate a small amount of grains (about an inch in the quart jars), let it colonize, and then shoot water into the jar, shake, and draw the myc water back up into the syringe to inoculate other jars. That way, you can know for sure if there are any contaminants. It can be difficult to tell with LC.

Heres a pictorial from agar: GRAIN LC

LC's are still good though and will probably work fine anyway.


--------------------

Don't hate the player; hate the game. -Abraham Lincoln


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InvisibleHorizonSpawn
Gettin' my grow on :)
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Re: Neuron's first grow :) [Re: Gzaajhom]
    #14567130 - 06/05/11 10:56 PM (12 years, 7 months ago)

Hmmmmm... :uhoh: If you plan on using the plastic lids, you might want to consider acquiring gaskets for them... Just a thought... I'm a total noob...



Oh... Almost forgot... I don't think I've ever seen a silicone-based glue rated lower than 400degF... You'll only be pressurizing to about 250degF...


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:


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Offlinedon_vedo
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Re: Neuron's first grow :) [Re: HorizonSpawn]
    #14567235 - 06/05/11 11:13 PM (12 years, 7 months ago)

Looks like your set up and getting ready to go! Happy your here brother, i'll definitely be following this thread; good vibes

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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OfflineShroom_i
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Re: Neuron's first grow :) [Re: neuron99]
    #14567358 - 06/05/11 11:39 PM (12 years, 7 months ago)

Oh I noticed that no one gae you the "strain" names.

Equador
Thai Lipa
Texas

Here's a good link about "strains" and why that word is misinforming.

Might as well get it now while you're new.

http://www.shroomery.org/forums/showflat.php/Number/7955834


--------------------
Prisoner#1 said:
[quote]destructo_low said:
Prisoner's posts by percentage.

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[/quote]

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Offlineneuron99
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Registered: 04/02/11
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Re: Neuron's first grow :) [Re: Shroom_i]
    #14567720 - 06/06/11 01:35 AM (12 years, 7 months ago)

Quote:

Shroom_i said:
Lookin good to me, I'll be following this.



Quote:

higgledy-piggledy said:
:congrats:

you are prepared.

(one of the very few people)




thanks! i'm a forum addict and am equally excited for all the learning to do and ppl to converse with as I am for my first harvest

Quote:

HorizonSpawn said:
Hmmmmm... :uhoh: If you plan on using the plastic lids, you might want to consider acquiring gaskets for them... Just a thought... I'm a total noob...



Oh... Almost forgot... I don't think I've ever seen a silicone-based glue rated lower than 400degF... You'll only be pressurizing to about 250degF...





I have both metal and plastic lids ATM and haven't drilled out anything yet, gonna do some more reading before settling on a lid design I like


Quote:

Gzaajhom said:
Looks good. I would suggest making a myc syringe instead of an LC though. Basically, what you do is inoculate a small amount of grains (about an inch in the quart jars), let it colonize, and then shoot water into the jar, shake, and draw the myc water back up into the syringe to inoculate other jars. That way, you can know for sure if there are any contaminants. It can be difficult to tell with LC.

Heres a pictorial from agar: GRAIN LC

LC's are still good though and will probably work fine anyway.




Unfortunately the link doesn't work for me, or I don't have permission to read it yet. I'll have to take a look into this!

Quote:

don_vedo said:
Looks like your set up and getting ready to go! Happy your here brother, i'll definitely be following this thread; good vibes

Lah'Kesh



Quote:

Shroom_i said:
Oh I noticed that no one gae you the "strain" names.

Equador
Thai Lipa
Texas

Here's a good link about "strains" and why that word is misinforming.

Might as well get it now while you're new.

http://www.shroomery.org/forums/showflat.php/Number/7955834




thank you thank you!!


So here's where I'm at, just finished building most of my glovebox, waiting for the silicone to set.  All that's left is to hoseclamp the gloves onto the couplings. 





Complete with warning sticker making sure I put no babies in my glovebox :tongue:




I put a Tyvek vent, not sure if this is essential or not, I did a quick forum search and decided on my own that it wouldn't hurt, esp with flame sterilization and alcohol being in the same container and the gloves themselves not being Tyvek but rubber instead.



Edited by neuron99 (06/06/11 01:43 AM)


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Offlineneuron99
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Re: Neuron's first grow :) [Re: neuron99]
    #14571528 - 06/06/11 09:04 PM (12 years, 7 months ago)

okay, the silicone is set and glove box is finally complete:



and WBS currently being soaked in tap water to fill 9 qt jars (3 jars @3qt x 3 races)

I'm following the 6T tek (http://www.shroomery.org/9032/6T-WBS-Tek-Wild-Bird-Seed-Bulk-Spawn-Preparation-Method)which simply says to soak and that floaters don't matter

however I've come across Doc's tek (http://www.shroomery.org/9030/Docs-Wild-Bird-Seed-Tek) which is much more involved and involves removing floaters as well as repeated washing and simmering.  I don't mind the additional labor if it will make a significant difference just curious to see what others think.





I've decided to directly inoculate the WBS with 6ml of the 10ml that I have (the 6ml split 2ml/qt) while saving the remaining 4ml to produce Honey LC for future use


Unfortunately my syringes are not marked in any matter so I will have to call them "1" "2" and "3" and visually identify them after fruiting

On order:
-12ml Luer Lock Syringe no needle 100box
-18 gauge needles x 100
(way more than I need lol)

I was eying some Sterilite 27qt boxes today, they are nice and clear with semi-clear tops.  I'm thinking of using them in conjunction with black trash bags for the final couple stages.  (3) 27qt boxes with 3qt of spawn in each, mixed with coco and coffee (ratios still undetermined)


Edited by neuron99 (06/06/11 09:27 PM)


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Offlinedon_vedo
MerKaBa
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Re: Neuron's first grow :) [Re: neuron99]
    #14571968 - 06/06/11 10:35 PM (12 years, 7 months ago)

Looks like you have put in a lot of time reading, that is definitely always good! Glovebox looks high tech, nice work with it man. From my experiences Doc's got some great TEK's, I personally have gone with his MSG for fruiting, couldn't say anything bad about it. I'm sure you'll have fantastic flushes! Good Vibes brother :mushroom2:!

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Offlineneuron99
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Re: Neuron's first grow :) [Re: don_vedo]
    #14572545 - 06/07/11 01:21 AM (12 years, 7 months ago)

just smoking some bho waiting for those WBS to soak...



I don't think I'm going to do the simmering but will give a good rinsing after a 12 hour soak, if anyone has objections please do chime in.


I'm starting to look ahead and plan the colonizing and fruiting stages.  I have a space in the closet I plan on using, I'm thinking of buying a plastic storage shelf unit and wrap with black/white poly film for the colonizing process and then place LED strip lighting underneath each shelf for lighting during fruiting.  Each shelf will have a MSG.

A cheaper alternative would be to use black trashbags for colonization and then use a clear plastic covering for the shelves during fruiting (for consistent humidity and addition barrier to contamination)

If I do make a somewhat sealed environment during fruiting, do I need to create some ventilation system for FAE? or would the air contained within be enough to circulate? they will be in MSG it just seems from what I've read a consistent RH is necessary for successful use


Edited by neuron99 (06/07/11 01:34 AM)


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OfflineTeemo
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Re: Neuron's first grow :) [Re: neuron99]
    #14572571 - 06/07/11 01:36 AM (12 years, 7 months ago)

Great job preparing and planning, Neuron!  :congrats:

I don't think I can be of much help, but I know that according to Doc's WBS tek, simmering helps the seed to absorb more moisture... and it works well for me.  I'm not sure how big of a difference simmering vs. not simmering makes, so hopefully someone else will chime in on that.

High humidity is important, but fresh air exchange is equally so.  It seems to me that if you were to use a plastic storage shelf, that would just create a lot of stale air, so you would definitely need a ventilation setup.  Although there'd be a lot of air in it, there'd be no air going out and no fresh air coming in.

Might I suggest you build a greenhouse in your closet with PVC pipe and plastic sheeting instead?  Or perhaps a martha?  You're doing trays, right?  Those would provide both the controlled humidity and FAE you want.

Good luck!  :nyan:


--------------------

"How terribly sad it was that people are made in such a way that they get used to something as extraordinary as living."


Edited by Teemo (06/07/11 01:38 AM)


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Offlineneuron99
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Re: Neuron's first grow :) [Re: Teemo]
    #14572600 - 06/07/11 01:50 AM (12 years, 7 months ago)

Thanks!

I was planning on using DocT's hybrid MSG, specifically the Sterilite tubs that are made for file hanging.

After reading, I think this is a good way to divide my risk and I think I'll be able to fit two tubs per shelf, each with 2 qts spawn.  (So instead of the 9qts I was originally planning I may have to up it to 12 qts...I soaked 10 dry qts so hopefully with the 20-25% expansion and 3/4 fill I'll get 12).

The MSG seem to be designed such that they can be used without additional housing but I also did read a member saying fluctuations in RH were quite detrimental to his grow, which is why I considered enclosing MSG within a humidity tent as advised.  Im just not sure what the essential features of a humidity tent are.

Yeah the PVC piping and sheeting is a good idea, I figured the shelving is on sale at Walmart for $14 and is likely to be structurally more rigid than something I put together.  I was debating about Black/White poly for privacy purposes but regular plastic sheeting would definitely be my second best alternative and equally effective.


Edited by neuron99 (06/07/11 03:47 AM)


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Offlinedon_vedo
MerKaBa
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Re: Neuron's first grow :) [Re: neuron99]
    #14574070 - 06/07/11 11:53 AM (12 years, 7 months ago)

Quote:

neuron99 said:
Yeah the PVC piping and sheeting is a good idea, I figured the shelving is on sale at Walmart for $14 and is likely to be structurally more rigid than something I put together.  I was debating about Black/White poly for privacy purposes but regular plastic sheeting would definitely be my second best alternative and equally effective.




Thanks for the info on the shelves brother, I think im going to pick one up myself and just throw the plastic sheeting over it. Then throw my humidifier on the bottom shelf. Seems pretty easy, and a great way to keep rh high without worrying about it dropping off when I am at work.

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Offlineneuron99
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Re: Neuron's first grow :) [Re: don_vedo]
    #14574848 - 06/07/11 02:44 PM (12 years, 7 months ago)

Quote:

don_vedo said:
Quote:

neuron99 said:
Yeah the PVC piping and sheeting is a good idea, I figured the shelving is on sale at Walmart for $14 and is likely to be structurally more rigid than something I put together.  I was debating about Black/White poly for privacy purposes but regular plastic sheeting would definitely be my second best alternative and equally effective.




Thanks for the info on the shelves brother, I think im going to pick one up myself and just throw the plastic sheeting over it. Then throw my humidifier on the bottom shelf. Seems pretty easy, and a great way to keep rh high without worrying about it dropping off when I am at work.

Lah'Kesh






You're very welcome! As a former cannabis cultivator my eyes are trained to scope out makeshift/McGuyver potential grow boxes (i.e. shelving units & storage cabinets) :crazy2:


WBS has been rinsed quite thoroughly and strained to dry after a 14 hour soak.  Having (2) 5 gallon buckets helped alot during the rinse.





Just finished drilling 5/16" holes into the quart jars.




Off to go fill my jars! I'm straining it one more time just in case...I think I did a fine job initially as I'm not seeing any more residual water form




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Offlinedon_vedo
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Re: Neuron's first grow :) [Re: neuron99]
    #14575159 - 06/07/11 03:47 PM (12 years, 7 months ago)

Looks great man, I love the updates. You must have been a great cannabis grower, I can tell you've definitely done your research thus far. I see alot of people coming here and just asking questions instead of taking the time to read through and find there own answers.

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Offlineneuron99
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Re: Neuron's first grow :) [Re: don_vedo]
    #14575979 - 06/07/11 06:42 PM (12 years, 7 months ago)

Quote:

don_vedo said:
Looks great man, I love the updates. You must have been a great cannabis grower, I can tell you've definitely done your research thus far. I see alot of people coming here and just asking questions instead of taking the time to read through and find there own answers.

Lah'Kesh




Thanks! I try to use the search engine when I can lol


So here's where I'm at now.

The pressure cooker can hold a maximum of 7 quart jars so I decided to process these jars in batches of 6:






While I wait for the first batch to complete, I started making some self-healing lids for future honey LC use.  Cutting holes out of a cardboard box helps with the drying of RTV silicone without touching any other surfaces:


These lids lack GE, see updated post for better design.






Looks like I have way more WBS than I need, I ended up filling additional 6 quarts (as planned) and another 6 pints (unplanned).

I'm still undecided what to do with the pint jars, I may use 3 of em and try a Grain LC.  Not sure what to do with the other 3.




I pressure cooked them for 90 minutes, while many people have PC'd for 60 minutes I figured the extra 30 minutes may be beneficial for me as I opted out of simmering the WBS initially.



After a rigorous, sweat-inducing shake, straight into the oven for cooling






Edited by neuron99 (08/17/11 03:34 PM)


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Offlineneuron99
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Re: Neuron's first grow :) [Re: neuron99]
    #14576589 - 06/07/11 08:42 PM (12 years, 7 months ago)

So I decided to not get ahead of myself and dumped out the pint jars.  I'm going to stick with the original plan of 2ml of MS directly inoculated into each 1QT WBS jar, using a total of 24ml of the 30ml spore solution that I have.  With the remaining 6ml (2ml each of 3 races) I will attempt to make honey LC in pint jars using 1ml, saving the last 1ml just in case my first attempt doesn't go well.


A days work done, 2 rounds of PC'ing, tomorrow morning is going to be exciting! Inoculation time! Get to use my glove box!




I'm not sure if I can stand to hear another couple hours of the PC's rattling, nor is the RTV completely set on the self-healing LC lids, so I'll be whipping up my honey LC and PC'ing it tmrw morning.


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Offlineneuron99
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Re: Neuron's first grow :) [Re: neuron99]
    #14578927 - 06/08/11 09:51 AM (12 years, 7 months ago)

Good mornin shoomery!


Today is inoculation day!  :dancer:

But first, will start brewing the honey LC as the RTV lids are finally finished (I'll be making the LC with the remaining solution after inoculating MS into WBS): See later posts for better LC lid design, ditched this one



Edited by neuron99 (08/17/11 03:33 PM)


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Offlinedon_vedo
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Re: Neuron's first grow :) [Re: neuron99]
    #14578950 - 06/08/11 09:59 AM (12 years, 7 months ago)

Nice keep up the updates brother, good vibes and good luck :mushroom2:

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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OfflineLoriath
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Re: Neuron's first grow :) [Re: don_vedo]
    #14579046 - 06/08/11 10:24 AM (12 years, 7 months ago)

Im really excited about this grow, you have done tons of research and for someone new at it you seem to have every aspect covered!

cant wait to watch those jars colonize.


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Offlineneuron99
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Re: Neuron's first grow :) [Re: Loriath]
    #14579067 - 06/08/11 10:31 AM (12 years, 7 months ago)

Okay so I whipped up the honey LC, a 4% solution so in these pint jars I used about ~10 ounces/~300 ml of water and ~2.5 teaspoons/~12.5 ml of clover honey. 

Gives me a few inches of space for shaking room as I don't have any broken glass or marbles on hand ATM (I had a whole box of broken borosilicate last month, little did I know I would have future use for it lol)

Anyways, into the PC they go! I'm using the single port RTV design lid so the mason jars have been tightened and then loosened 1/4 turn.




As a reminder to myself, I'm gonna need to use the empty syringe+iso soaked poly method to de-pressurize before inoculating.  Planning on 1cc in each jar.


Cleaned out an empty cabinet to prepare for storage.  Man, this is a good hobby to have for a stoner, I don't think I've cleaned my house like this ever before.

The cabinet, completely empty, is at a constant 80*F.  IIRC, the jars themselves will be producing some heat, I hope that this environment is ideal.




Cooled down WBS jars now patiently awaiting inoculation, I gotta go run an errand soon, gonna wait until the afternoon when I have a nice chunk of time so nothings rushed.



Edited by neuron99 (08/17/11 03:35 PM)


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Offlinesporophight
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Re: Neuron's first grow :) [Re: neuron99]
    #14579185 - 06/08/11 10:59 AM (12 years, 7 months ago)

First off, Great job being well informed and thorough. As far as shelving goes, If you are using a tub I would advise against making a sealed chamber as well. It is redundant and possibly detrimental. If you think that humidity fluctuations are going to be an issue then i would abandon the tub and use trays in a greenhouse/martha. Congrats on the new hobby! Good luck!:thumbup::thumbup:


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Re: Neuron's first grow :) [Re: sporophight]
    #14579280 - 06/08/11 11:24 AM (12 years, 7 months ago)

Quote:

sporophight said:
First off, Great job being well informed and thorough. As far as shelving goes, If you are using a tub I would advise against making a sealed chamber as well. It is redundant and possibly detrimental. If you think that humidity fluctuations are going to be an issue then i would abandon the tub and use trays in a greenhouse/martha. Congrats on the new hobby! Good luck!:thumbup::thumbup:




I think neuron was going to use a msg type tub which is a monotub with small holes drilled in 5 sides kind of like a shotgun fc; he was going to use a sealed chamber to keep rh higher due to the fact that the msg might produce to much fae and dry the sub out in a normal room.

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Re: Neuron's first grow :) [Re: neuron99]
    #14579297 - 06/08/11 11:28 AM (12 years, 7 months ago)

Quote:

neuron99 said:
... I hope that this environment is ideal.



with the doors of your cabinet open your jars get nice ambient light.

you also could dismantle them...


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Re: Neuron's first grow :) [Re: higgledy-piggledy]
    #14579376 - 06/08/11 11:46 AM (12 years, 7 months ago)

Quote:

higgledy-piggledy said:
Quote:

neuron99 said:
... I hope that this environment is ideal.



with the doors of your cabinet open your jars get nice ambient light.

you also could dismantle them...




for colonization do I need ambient light?  I was planning on closing the cabinet and forgetting about it for a couple weeks to allow for colonization


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Re: Neuron's first grow :) [Re: neuron99]
    #14579408 - 06/08/11 11:52 AM (12 years, 7 months ago)

Quote:

neuron99 said:
for colonization do I need ambient light?  I was planning on closing the cabinet and forgetting about it for a couple weeks to allow for colonization




I have bags going great right now with no ambient light, only light they get is from me checking them every once in a while. I think it could go either way.

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Re: Neuron's first grow :) [Re: don_vedo]
    #14579425 - 06/08/11 11:57 AM (12 years, 7 months ago)

okay cool I was under the impression that darkness is ideal for colonization and ambient light should  be introduced to induce pinning

I don't plan on fruiting in that cabinet, first the space is way too limited for the 12 qts of spawn I'm creating, additionally it's above my fridge and sort of a PITA to access.  Good for privacy, not for when things get bulky.


I just finished PC'ing the honey water for 15 minutes @ 15psi, however I'm afraid that I overcooked the honey. Is this what it's supposed to look like? I don't recall seeing so much sediment in others, while I have read a couple members post saying they coffee filter their honey water.  Am I going to be okay inoculating these as soon as they cool down?








Edited by neuron99 (06/08/11 12:01 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14580396 - 06/08/11 03:25 PM (12 years, 7 months ago)

Inoculated 2cc into each 1qt jar of WBS:



















(not pictured: wiping of hot needle w/ alcohol soaked paper towel)











After a quick shake, back into the cabinet they go:




See you in 2~3 weeks!



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Re: Neuron's first grow :) [Re: neuron99]
    #14580427 - 06/08/11 03:31 PM (12 years, 7 months ago)

Nice job brother, good vibes comin your way :mushroom2:

Can't wait to see the progress!

Lah'kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Re: Neuron's first grow :) [Re: don_vedo]
    #14581259 - 06/08/11 06:40 PM (12 years, 7 months ago)

you sanitized a sterilized needle.

you went backwards >.>



probs wont hurt

but to cool needles. you expel a drop of spore solution.
dont touch your needle with anything.


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Re: Neuron's first grow :) [Re: k00laid]
    #14581758 - 06/08/11 08:35 PM (12 years, 7 months ago)

I was following Doc's WBS tek, specifically the inoculation steps where he advises flame sterilization followed by alcohol wipe before inoculating the jar and then another alcohol wipe after injection


But either way, thanks for the heads up, I don't think one can be too sterile :smile:


Edited by neuron99 (06/08/11 08:36 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14581781 - 06/08/11 08:39 PM (12 years, 7 months ago)

well. once you learn the difference between sanitizing and sterilizing you will know why you wiping with alcohol can actually be detrimental.

but im just being nit picky.
people do it that way all the time.

xD


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OfflineSelfindulgence
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Re: Neuron's first grow :) [Re: k00laid]
    #14581982 - 06/08/11 09:14 PM (12 years, 7 months ago)

I realize it seems to be echoing through this thread but amazing first grow. You've inspired me to change my entire approach on my next grow. Awesome attention to detail brother.


--------------------
Fuck L Ron Hubbard and Fuck all his clones. Fuck all those gun-toting Hip gangster wannabes. Fuck retro anything. Fuck your tattoos. Fuck all you junkies and Fuck your short memory. Fuck smiley glad-hands With hidden agendas. Fuck these dysfunctional, Insecure actresses. Learn to swim. Cuz I'm praying for rain and I'm praying for tidal waves I wanna see the ground give way. I wanna watch it all go down. Mom please flush it all away. I wanna watch it go right in and down. I wanna watch it go right in. Watch you flush it all away. Time to bring it down again. Don't just call me pessimist. Try and read between the lines. I can't imagine why you wouldn't Welcome any change, my friend. I wanna see it all come down. suck it down. flush it down.


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Re: Neuron's first grow :) [Re: don_vedo]
    #14584038 - 06/09/11 08:17 AM (12 years, 7 months ago)

Quote:

don_vedo said:
Quote:

sporophight said:
First off, Great job being well informed and thorough. As far as shelving goes, If you are using a tub I would advise against making a sealed chamber as well. It is redundant and possibly detrimental. If you think that humidity fluctuations are going to be an issue then i would abandon the tub and use trays in a greenhouse/martha. Congrats on the new hobby! Good luck!:thumbup::thumbup:




I think neuron was going to use a msg type tub which is a monotub with small holes drilled in 5 sides kind of like a shotgun fc; he was going to use a sealed chamber to keep rh higher due to the fact that the msg might produce to much fae and dry the sub out in a normal room.

Lah'Kesh




I realize what a MSG is. I think that instead of combining completely different teks you should just adjust the number/size of holes. No single tub design will work in every environment. But with adjustments a tub can work in most.


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Re: Neuron's first grow :) [Re: sporophight]
    #14584770 - 06/09/11 10:41 AM (12 years, 7 months ago)

Oh sorry thought you didn't realize it was an msg; I stated that, because after reading, I have found that doc T and several others have had great success with fruiting msg's in a gh or some sort of humidity tent.

Totally understand what your saying, definitely makes sense. I would imagine to each his own. Personally for me I am going to go with fruiting my msg's in a gh just cause the method has been proven to work and I don't have enough time in my daily life right now to experiment as much as id like.

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Re: Neuron's first grow :) [Re: don_vedo]
    #14588144 - 06/09/11 10:34 PM (12 years, 7 months ago)

Yup I think I'm leaning towards a MSG in that shelf I posted up but setup like a GH with plastic lining, it would be semi-sealed


My glassblowing friend kindly donated me some material for my future LC jars :smile: Nice and sharp to break up the mycelium




And on order are some Whatman syringe filters for better LC lids, the Tyvek and polyfill designs make me concerned about wicking up contamination when I shake the jar

Should I check on the WBS jars periodically (1/week)  or would it be better to just wait the 3 weeks?  Does momentary ambient light exposure affect colonization at all?


Edited by neuron99 (06/09/11 10:44 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14588201 - 06/09/11 10:46 PM (12 years, 7 months ago)

Nice buddy you got there!

I am doing some MSG tubs myself, how were you planning on doing your lighting with the shelves? Just not snapping the top shelf on? I think I am going to go with this greenhouse I found at Lowes.

http://www.lowes.com/pd_318839-10155-FHPT400CL_4294935924_4294937087_?productId=3111999&Ns=p_product_prd_lis_ord_nbr|0||p_product_quantity_sold|1&pl=1¤tURL=%2Fpl_Greenhouses_4294935924_4294937087_%3FNs%3Dp_product_prd_lis_ord_nbr|0||p_product_quantity_sold|1&facetInfo=

Just so I don't have to put one together myself, and i'm thinking it would end up being a good investment as I can use it for a long time, with several grows.

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Re: Neuron's first grow :) [Re: don_vedo]
    #14588220 - 06/09/11 10:51 PM (12 years, 7 months ago)

Cool! That's the design I'll be trying to mimic, hopefully a DIY version will have significant savings with equal performance, if not I'll definitely ditch it for the Lowes one.  I'm thinking of using black plastic for additional privacy.

As for lighting I was eyeing high output LED strip lighting or some low wattage under cabinet floro tubes (IIRC they come around ~12") each level would have it's own light.  I'm leaning towards LED as long as it's not cost prohibitive


Edited by neuron99 (06/09/11 10:53 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14588315 - 06/09/11 11:15 PM (12 years, 7 months ago)

Good thinking on the lights, I think I am going to use a couple 6500k fluorescent bulbs, I have some lamps I was able to modify so that I can just hang them over the greenhouse itself and let the light shine in through the clear viynile. 

Keep posting those pics man, love the step by step process! Watchin your every move :grin:

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Re: Neuron's first grow :) [Re: don_vedo]
    #14588351 - 06/09/11 11:24 PM (12 years, 7 months ago)

What I don't understand, is it seems that you made a hella over the top glove box(very cool by the way). Yet your jars only are protected by coffee filters on top, no micopore tape, tyvek, or polyfill. I always thought coffee filters were more of an extra not a main. I always follow docs wbs tek to the max, by adding polyfill to the inoculation points, tyvek over that, and coffee filter after i poke a hole through the tyvek. I'm sure It's just me being super anal, I'm just confused at how you seem to have spent mad time on the glove box(mine is just a box with 2 holes haha) yet not much extra time on adding as much protection to the injection points as possible. I dunno you could of added tape after inoculating, I couldn't tell. Good luck buddy.


--------------------
Grain Jar to Homemade Mycobag Tek
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Re: Neuron's first grow :) [Re: FuckMeRunnin]
    #14588369 - 06/09/11 11:30 PM (12 years, 7 months ago)

Quote:

FuckMeRunnin said:
What I don't understand, is it seems that you made a hella over the top glove box(very cool by the way). Yet your jars only are protected by coffee filters on top, no micopore tape, tyvek, or polyfill. I always thought coffee filters were more of an extra not a main. I always follow docs wbs tek to the max, by adding polyfill to the inoculation points, tyvek over that, and coffee filter after i poke a hole through the tyvek. I'm sure It's just me being super anal, I'm just confused at how you seem to have spent mad time on the glove box(mine is just a box with 2 holes haha) yet not much extra time on adding as much protection to the injection points as possible. I dunno you could of added tape after inoculating, I couldn't tell. Good luck buddy.




:confused2: I think he used silicone injection holes so he wouldn't need polyfill.. also I've heard that tyvek + polyfill + coffee filters restricts FAE which is still important in colonizing myc.  I know I've posted pics of my jars a few times here in cult (I do polyfill + coffee filters) and I've been scolded and told to take off the coffee filters, since they really wouldn't block any trich or other mold spores, and hinder my efforts by restricting FAE.  (I usually leave my coffee filters on until they're mostly colonized though, just cuz I've got a dusty house)


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Re: Neuron's first grow :) [Re: FuckMeRunnin]
    #14588396 - 06/09/11 11:36 PM (12 years, 7 months ago)

I thought that he added that stuff to the lc jars not the grain. I could be wrong, and wouldn't self healing silicon holes prevent FAE more so then 3 layers of material? The reason I say this is because the LC tek i followed called for the back of a syringe filled with polyfill, next to the self healing injection point. This way you wouldn't have to manually pump air into the jar with a needle every so often.


--------------------
Grain Jar to Homemade Mycobag Tek
http://www.shroomery.org/forums/showflat.php/Number/10954174


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Re: Neuron's first grow :) [Re: FuckMeRunnin]
    #14588518 - 06/10/11 12:15 AM (12 years, 7 months ago)

The WBS jars have Tyvek + micropore tape over injection site in tyvek, and double coffee filter


The 1st version LC lids had the silicone port but I dumped the LC jars pictured and am modifying the lids to also include Whatman syring filter for GE so I don't have to depressurize with an amply sterilized syringe


Edited by neuron99 (06/10/11 01:09 AM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14588537 - 06/10/11 12:23 AM (12 years, 7 months ago)

Yes, the syringe filter idea was genius, I have used it many times and never had a problem...Hell I forgot about one over a year and a half ago, I recently inoculated jars and low and behold no contams :-) ps why didn't you just keep the tyvek on, under the jar ring? Then again with the box you have going there's nothing getting in haha.


--------------------
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http://www.shroomery.org/forums/showflat.php/Number/10954174


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Re: Neuron's first grow :) [Re: FuckMeRunnin]
    #14588659 - 06/10/11 01:10 AM (12 years, 7 months ago)

the Tyvek is placed over the glass mason jar, under the lid w/ holes, both under the screw top collar


Edited by neuron99 (06/10/11 01:11 AM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14623554 - 06/16/11 03:57 PM (12 years, 7 months ago)

It's been about a week so I took a peek to see how things are going, the Tx seem to have started colonizing, I can see faint patches of whitish hairs formimg, the Lip and Eq don't show any signs yet.


Some goodies arrived in the mail:






Still waiting on the Whatman filters, once they get in it's time to whip up some LC jars


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Re: Neuron's first grow :) [Re: neuron99]
    #14623629 - 06/16/11 04:08 PM (12 years, 7 months ago)

Looks good neuron, I normally do bags but usually once I start seeing growth they take off from there and don't look back. My current grow, the bags showed signs in 7 days and then 7 days later they were 100%. I spawned to bulk Tuesday night so I'll give them a week until I take a peek. My point being that they should start rolling now for you! Good luck and good vibes brother, keep us posted on your LC.

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Re: Neuron's first grow :) [Re: don_vedo]
    #14642953 - 06/20/11 12:56 PM (12 years, 7 months ago)

Been checking up on the jars periodically, they have all started to colonize, Tx being much faster than the other two.


:sad: unfortunately I think I've spotted a contamination in one of my Tx jars, can someone confirm:






should I toss the jar? or dump it outdoors somewhere


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Offlinejpshroomer
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Re: Neuron's first grow :) [Re: neuron99]
    #14643007 - 06/20/11 01:06 PM (12 years, 7 months ago)

it looks like trich to me. def toss it or put outside see what happens but get it away from the others...


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Re: Neuron's first grow :) [Re: jpshroomer]
    #14643177 - 06/20/11 01:33 PM (12 years, 7 months ago)

damn, I found another jar with a tiny green speck.  makes that 2/12 jars, both Texans.

I feel like a dirty person lol contaminated even with a glovebox


On the plus, the others are nice and healthy white.  But only about 5% colonized (generous estimate).  Will need to check end of the month.


Edited by neuron99 (06/20/11 01:34 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14643704 - 06/20/11 03:16 PM (12 years, 7 months ago)

Yah I would definitely say trich and would say to get them away from your grow area ASAP brother, no reason to get the those spores all over the place! Personally would toss the jars but I guess you could throw them outdoors and see why happens.

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Offlineneuron99
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Re: Neuron's first grow :) [Re: don_vedo]
    #14710840 - 07/03/11 12:32 PM (12 years, 6 months ago)

Here's a quick update:









I'm a bit concerned about this jar, it seems to have quite a bit of moisture but I don't see anything discolored.  All the growth seems to be bright white.





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Re: Neuron's first grow :) [Re: neuron99]
    #14711119 - 07/03/11 01:41 PM (12 years, 6 months ago)

It looks like the last one has a bacterial infection. The 7x may also have a contaminant but its hard to tell. Bacteria makes the grains look wet/slimy, and when you shake it up the grains that were sticking to the glass will leave a brown residue.


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Re: Neuron's first grow :) [Re: Gzaajhom]
    #14711223 - 07/03/11 02:04 PM (12 years, 6 months ago)

Hrm okay, those are the Texans, labeled "Tx"

Should I toss those jars out? What would have caused the infection?


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Re: Neuron's first grow :) [Re: neuron99]
    #14715362 - 07/04/11 11:56 AM (12 years, 6 months ago)

so I've moved the jars into a cooler environment and it seems that growth has perked up a bit, I'm thinking inside the cabinet was too warm


do I toss all jars with brown liquid residue or only the really bad one (pictured)?  It looks it could be burst "wbs juice" as well from shaking


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Re: Neuron's first grow :) [Re: neuron99]
    #14721032 - 07/05/11 03:20 PM (12 years, 6 months ago)

I would let it go and see what it does, most likely bacteria but why not just let it finish up. Open the it outside or away from your grow area; depending on the smell Ull know what's going on. I would post a thread in the contam section of the forum as well to get some advice from the guys that hang over that way.

Lah'Kesh


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Re: Neuron's first grow :) [Re: don_vedo]
    #14728130 - 07/06/11 08:25 PM (12 years, 6 months ago)

I pulled three more jars out due to excessive brown colored moisture, they're not dumped yet bu quarantined outside.  However I doubt any will be useable just gonna dump em in a shady spot


I believe the culprit is me shaking the jars (such that moist WBS came in contact with the Tyvek, thus wicking contams) as well as too warm of temperatures 80*F+ outside the cab so inside must've been a few degrees higher


Gonna go and get materials for FC, looks like Ill be working with 7 qts of spawn...


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Re: Neuron's first grow :) [Re: neuron99]
    #14728331 - 07/06/11 09:04 PM (12 years, 6 months ago)

Quote:

neuron99 said:
I pulled three more jars out due to excessive brown colored moisture, they're not dumped yet bu quarantined outside.  However I doubt any will be useable just gonna dump em in a shady spot


I believe the culprit is me shaking the jars (such that moist WBS came in contact with the Tyvek, thus wicking contams) as well as too warm of temperatures 80*F+ outside the cab so inside must've been a few degrees higher


Gonna go and get materials for FC, looks like Ill be working with 7 qts of spawn...




Don't get down on yourself, it was probably a combination of the heat and shaking. I find my bags start churning along nicely at about 70-75*F.

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Re: Neuron's first grow :) [Re: don_vedo]
    #14736156 - 07/08/11 12:43 PM (12 years, 6 months ago)

thanks, this summer heat isn't helping :sun::thumbdown:


unfortunately no pictures, but I spawned 3QTS of Thai Lipa into 2 hybrid shotgun monotubs last night, each one has roughly 1.5 QTS of spawn to about 4 QTS of coir/verm/coffee


have to admit, the transfer of spawn into tubs made me a little nervous of contamination...I did wipe the entire workspace down as well as spray aerosol lysol spray prior to transfer.  made sure to wear latex gloves and cleaned my spoon with ISO.  wish I had a giant cleanroom haha


after transferring spawn, black trash bagged and waiting now :dancer:



also got 4 QTS of Equador finishing up colonizing in jars which I'll make sure to document when I transfer to their tub(s)


all the Texans eventually got quarantined due to infection/contamination


Edited by neuron99 (07/08/11 12:55 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14736220 - 07/08/11 12:58 PM (12 years, 6 months ago)

I just harvested my first flushes from my MSGs. They produced a decent amount however I was not to happy with the side pinning I got; I ended up going without a trashbag and taped the sides, the sub shrank really quickly and side pins were almost inevitable. I fruited in my Martha which kept a decent humidity which kept things a little more constant.

One piece of advise I would give to you is make sure you let that sub colonize and then consolidate till you see pins, it will be well worth it. From my experience you will wait regardless if your fruit early or not. Personally I am going to try bulk trays next. I have done monos, now done msg and think I might prefer just normal trays in the Martha.

Keep the updates coming neuron! Good luck brother!

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Re: Neuron's first grow :) [Re: don_vedo]
    #14736243 - 07/08/11 01:02 PM (12 years, 6 months ago)

thanks for the tip! I don't wanna be a peeker, approximately how many days do you think I should wait? 

I also opted out of trashbagging the inside and just duct taped the bottom 4.5", we'll see how the side pinning turns out.  For the remaining spawn I'll line the inside with bag or foil and see how that works out.


I too want to eventually have trays in a Martha setup, while it has a little more startup cost involved it looks to be quite a stable environment


Still need to make some LC lids and make a batch of LC, I'm a bit wary of contamination, do you guys think it's better to use the remaining MS solution to inoc a grain jar to do a G2G or create a LC solution? IIRC it's very difficult to determine if a LC jar is contaminated or not?


Edited by neuron99 (07/08/11 01:12 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14737160 - 07/08/11 04:32 PM (12 years, 6 months ago)

I would wait like 12-15 days before peeking and then after you peek if you still don't have pins put it back and leave to consolidate until pins appear. The mcy will force pins even in colonization after so much time. I believe that was my problem, I fruited to early then when pins did appear they appeared on the best climate avaiable to them; without a trashbag liner and being that the sub pulled away from the sides I ended up with mostly side pins. Grant you I got a great flush but harvest has been a pain in the ass.

Martha with trays definitely seems to be the best available option in my opinion. I have bags colonizing now and will spawn to bulk in trays when they are done and then throw them in my martha. Like I said tubs are just a pain in my ass; and from my experience they aren't as easy as just spawning an then giving them no care till harvest.

If it were me I would make an LC and instead of attempting G2G from all that I have read G2Gs really require a flow hood or at least you attain the best chance of getting no contams with one.

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Re: Neuron's first grow :) [Re: don_vedo]
    #14876276 - 08/05/11 10:53 AM (12 years, 5 months ago)

looks like the Thai Lipa are almost done colonizing, I did a pretty low ratio of spawn:bulk like near 1:4 but everything looks good.

it's interesting to observe different rates of colonization for the same spawn in different tubs.  one tub is completely covered in white and colonized while the other seems to be a bit more patchy

the Equador was spawned using a 1:1 ratio and is also finishing up (tub was made later than the rest, 7/21)


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Re: Neuron's first grow :) [Re: neuron99]
    #14877676 - 08/05/11 04:34 PM (12 years, 5 months ago)

Definitely post some pics when you start fruiting an seeing pins! Thanks for the update brother, been wondering how this grows been goin.

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Re: Neuron's first grow :) [Re: don_vedo]
    #14878433 - 08/05/11 07:31 PM (12 years, 5 months ago)

finally got some mushroom related work done today, LC lids are ready to be used:




and built a fruiting chamber for these monotub/shotgun hybrids:


(i love zip ties btw)

















pretty budget setup, lights from IKEA @ $9.99 ea and the shelves at Home Depot for $11, I forget exactly how much drop cloth and zip ties were, but those aren't very expensive

once I get a chance I'll probably neatly secure portions of the drop cloth to the frame, right now I'm feeling lazy and just draped it over

I'm not trying to built a Martha at the moment, just a greenhouse that can hold up the RH for my monotub/shotgun hybrids as RH tends to fluctuate around my house and if anything on the dry end


Edited by neuron99 (08/05/11 07:36 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14879091 - 08/05/11 10:19 PM (12 years, 5 months ago)

these tubs have been colonizing for what seems to be forever, the first two have been colonizing for 28 days, the third one has been colonizing for 15 days. everything was WBS spawn to bulk Coco/Verm/Coffee mixture.  ratio for the first two tubs was about 1:4 and the third tub was 1:1


I'm thinking the first tub is ready to be unbagged and placed in the FC with a wax paper casing?


1.)Thai Lipa #1 (28 days)




2.)Thai Lipa #2 (28 days)




3.)Equador (15 days)



Edited by neuron99 (08/06/11 01:22 AM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14880535 - 08/06/11 10:12 AM (12 years, 5 months ago)

bump... should I try fruiting the first tub or wait a bit longer till its more densely covered with growth?


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Re: Neuron's first grow :) [Re: neuron99]
    #14880728 - 08/06/11 11:15 AM (12 years, 5 months ago)

Fruit that first tray, the other two you should definitely wait.

How did you prepare your substrate? What temperatures are those tubs kept around during their spawn run?


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Re: Neuron's first grow :) [Re: thatguy0]
    #14881387 - 08/06/11 02:36 PM (12 years, 5 months ago)

Just in case, as you may not know... Be sure to get a gasket for those plastic LC lids. As they are neither air nor liquid tight without a proper gasket.


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Re: Neuron's first grow :) [Re: HorizonSpawn]
    #14881911 - 08/06/11 05:07 PM (12 years, 5 months ago)

I was planning on using a wide-band rubberband as a gasket, do you think that will be sufficient?

the spawn were MS syringe inoculated in WBS.  bulk substrate was a coco/verm/coffee mix around 70/20/10.  set to colonize in black garbage bags in temps I would approximate to be low 80's


Edited by neuron99 (08/06/11 05:30 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14885223 - 08/07/11 12:00 PM (12 years, 5 months ago)

That first one looks good to go brother, however you could always wait till it starts showing pins to introduce to fruiting. After this advice was given to me I have found my first flushes to be a lot more prolific.

Lah'Kesh


--------------------
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Re: Neuron's first grow :) [Re: don_vedo]
    #14885437 - 08/07/11 01:00 PM (12 years, 5 months ago)

Quote:

don_vedo said:
That first one looks good to go brother, however you could always wait till it starts showing pins to introduce to fruiting. After this advice was given to me I have found my first flushes to be a lot more prolific.

Lah'Kesh





awesome :smile: it's basking in CFL light getting misted and covered with crumpled parchment paper

have you noticed the myc pinning even before FAE and light is introduced?


off to PC some honey!


Edited by neuron99 (08/07/11 01:12 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14886552 - 08/07/11 04:58 PM (12 years, 5 months ago)

Yah the myc will eventually force pins if left in colonization for a period of time.

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Re: Neuron's first grow :) [Re: don_vedo]
    #14915636 - 08/13/11 12:01 PM (12 years, 5 months ago)

here's an update on how the tubs are doing, since this is my first time I'm not 100% on identifying knots and primordia formation





i hope i see pins soon! there seems to be alot of metabolite build up.  i've been fanning & misting only 2x a day, approx 12h apart and placing crumpled wax paper over the surface.

box #1:




box #2:





think this box is ready to fruit?:





oh and off topic, but looks what I found at the local farmers market :dancer: :bow2::dancer:



Edited by neuron99 (08/13/11 12:17 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14915666 - 08/13/11 12:11 PM (12 years, 5 months ago)

your first two pics are metabolites. maybe not enough fresh air.

the second is some weird mycelium, looks infected, but you'll know in a few days.

put holes in the plastic sheet, or just fan it out ~3 times a day.


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Re: Neuron's first grow :) [Re: k00laid]
    #14915712 - 08/13/11 12:26 PM (12 years, 5 months ago)

ok cool thanks for the speedy reply, I've moved the plastic over so there's a nice slot for FAE





what is it about the second box that makes you believe theres an infection?  i'm curious to learn how to "read" the mycellium but at this point most of the boxes look the same to me lol...


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Re: Neuron's first grow :) [Re: neuron99]
    #14915965 - 08/13/11 01:46 PM (12 years, 5 months ago)

:shrug: looks weird. it might fruit,,


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Re: Neuron's first grow :) [Re: k00laid]
    #14920364 - 08/14/11 11:36 AM (12 years, 5 months ago)

I agree with k00laide, that 3rd tubs myc. looks a little funny. The top 2 are definitely loaded with metabolites; I would guess your tubs are a little too wet and is why you are seeing pooling and metabolites like that. Definitely get that FAE up.


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Re: Neuron's first grow :) [Re: don_vedo]
    #14920523 - 08/14/11 12:06 PM (12 years, 5 months ago)

hmmm okay I hope it turns out to make it


been focusing on giving the tubs a good fanning 3-4x a day and misting only in the morning


i'm getting nervous, the tubs have been in the FC for a week now and I still don't see pinning  :facepalm3:


Edited by neuron99 (08/14/11 12:07 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14920601 - 08/14/11 12:21 PM (12 years, 5 months ago)

Just let them be and they will be. The first time I went through the whole process I was nervous around every bend. Give it time. Next time I would let the tubs colonize and consolidate till they show signs of either knots or pins.

Lah'Kesh


--------------------
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Re: Neuron's first grow :) [Re: don_vedo]
    #14920624 - 08/14/11 12:26 PM (12 years, 5 months ago)

I'm hoping the next run will go better, and probably faster if I start with an LC instead of MS syringe.


Am I dealing with any "overlay" with my tubs?  I've been doing mad amounts of reading trying to figure out the best thing to do, but not experienced enough to tell whether its myc continuing to colonize or overlay


I've been noticing small pools of metabolites and I've been just tilting my tubs in different directions until the substrate has reabsorbed the metabolite moisture.  I figured this would be better than attempting to wick up the excess?


Edited by neuron99 (08/14/11 12:28 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14920658 - 08/14/11 12:34 PM (12 years, 5 months ago)

Your next time should definitely go better. This round you have learned a lot and have gone through the motions so that next time you will be able to make adjustments. LC batches should definitely go by faster,I have found that a straight water LC is the best way to go.

I don't think your dealing with overlay but can't be 100% sure, from my experience reading overlay is pretty rare and doesn't come around too often.

Personally when my tubs get metabolites like that I start to soak them up with the corners of paper towels. For me metabolites always indicate too moist of conditions.

Lah'kesh


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Re: Neuron's first grow :) [Re: don_vedo]
    #14921261 - 08/14/11 03:03 PM (12 years, 5 months ago)

the window the MSG's are sitting next to is half open (not the half nearest the tubs) so there is some air flow.  hygrometer right next to the MSG has been right around 60% these days.

i've been placing the crumpled wax paper over the substrate to keep local RH% high.

hopefully these conditions are ideal for a MSG tub, i've been leaving the shelves mostly uncovered to get good FAE w/o noticing a drop in RH%.


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Re: Neuron's first grow :) [Re: neuron99]
    #14925524 - 08/15/11 11:31 AM (12 years, 5 months ago)

I have my first set of pins in tub#1!!






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Re: Neuron's first grow :) [Re: neuron99]
    #14925535 - 08/15/11 11:33 AM (12 years, 5 months ago)

:popcorn:


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Re: Neuron's first grow :) [Re: Fungus_monk]
    #14925603 - 08/15/11 11:48 AM (12 years, 5 months ago)

awwww yeah!


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Re: Neuron's first grow :) [Re: k00laid]
    #14928506 - 08/15/11 09:01 PM (12 years, 5 months ago)

:bender:

Lah'Kesh


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Re: Neuron's first grow :) [Re: don_vedo]
    #14937323 - 08/17/11 01:31 PM (12 years, 5 months ago)

thanks guys! i'm soooooo excited  :biggrin:



Had this posted over at the contamination forum but wasn't getting any responses...

MS syringe of Equador inoculated in WBS and spawned to Coir/Verm/Coffee MSG tub

Been bagged up and sitting waiting for 100% colonization before fruiting, noticed weird yellow fuzz today.


I don't have much experience, but this didn't look like the metabolites in my other tubs so curious to see what this is and if I can fix it with H2O2 if it indeed is a contam


tub #3



Edited by neuron99 (08/17/11 03:29 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14937860 - 08/17/11 03:25 PM (12 years, 5 months ago)

^
|
|

Still got that question unanswered, but here's some pinporn from tub #1 :smile: third day of fruiting







largest fruits in the tub so far:



a baby pin next to slightly more mature fruit:




pin just started, about 3mm in diameter:



Edited by neuron99 (08/17/11 03:36 PM)


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Re: Neuron's first grow :) [Re: neuron99]
    #14942536 - 08/18/11 01:19 PM (12 years, 5 months ago)

Hey Neuron, not sure I can help you out with the possible contam problem with tub #3. However I am very excited for you, it's always awesome to start seeing pins poppin up. Looks like some of those tubs are going to push out side pins like crazy; I wouldnt worry but next time definitely use a trash bag. Same thing happened to me my last go around with the MSG, side pinning isn't too bad just a pain in the ass. Also looks like some of those caps might be a little too moist but I could be wrong; with the metabolites and moist looking caps I'd def say cool it on the misting for a couple days just so the tubs dry out a little. Keep those updates rollin brother!

Lah'Kesh


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Re: Neuron's first grow :) [Re: don_vedo]
    #14942720 - 08/18/11 01:59 PM (12 years, 5 months ago)

cool thanks for the pointer, check out the dimpled cap few pix down, is that what you mean?


man the rate of growth when fruiting is so fun, every morning feels like christmas morning









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Offlineneuron99
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Re: Neuron's first grow :) [Re: neuron99]
    #14947191 - 08/19/11 12:06 PM (12 years, 5 months ago)

looks like tub #3 (still colonizing in closet) got hit with trich, the yellow funkiness has taken on a shade of green :frown:


but good news, I woke up to this today :smile:




looks like somebody had a wet dream last night...  :drysmash:





veils broken, others starting to split.... harvest time!








1st flush: 76.1g (wet)



some other good news, saw my first pin in tub #2 today:






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Offlinedon_vedo
MerKaBa
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Registered: 05/12/11
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Re: Neuron's first grow :) [Re: neuron99]
    #14947424 - 08/19/11 12:48 PM (12 years, 5 months ago)

Nice, enjoy that fresh harvest! Sorry to hear about the trich brother!

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Offlineneuron99
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Re: Neuron's first grow :) [Re: don_vedo]
    #14952020 - 08/20/11 10:49 AM (12 years, 5 months ago)

thanks!!

been harvesting a few mushrooms here and there, been keeping an eye and trying to catch them right as their veils start breaking.  is that okay to do, or should I harvest in staged increments?





today's cut:



Edited by neuron99 (08/20/11 11:01 AM)


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Offlineeric owns


Registered: 07/15/11
Posts: 708
Last seen: 7 months, 6 days
Re: Neuron's first grow :) [Re: neuron99]
    #14952096 - 08/20/11 11:18 AM (12 years, 5 months ago)

Dam your going mightly fine this pics are awesome!
i solute you


--------------------



"I felt sorry for the living to know such sarrow. And death being able to heal the scars that everyone swore time would heal" - Eric owns


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Offlineneuron99
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Re: Neuron's first grow :) [Re: eric owns]
    #14952602 - 08/20/11 01:42 PM (12 years, 5 months ago)

thanks! :mushroom2:


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Offlineneuron99
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Re: Neuron's first grow :) [Re: neuron99]
    #14955980 - 08/21/11 10:01 AM (12 years, 5 months ago)

harvested these beauties this morning:





My 2-stage drying setup, here's stage 1:

desk fan blowing straight up, bamboo kabob skewers poked thru a cardboard box




stage 2: 5-gallon bucket with damprid flakes on the bottom and cardboard insert to hold mushrooms




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Offlineneuron99
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Re: Neuron's first grow :) *DELETED* [Re: neuron99]
    #14957904 - 08/21/11 06:37 PM (12 years, 5 months ago)

Post deleted by neuron99

Reason for deletion: before 24



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Invisibledtowntoker
gimme a spliff
Registered: 08/06/11
Posts: 2,368
Re: Neuron's first grow :) [Re: neuron99]
    #14957918 - 08/21/11 06:42 PM (12 years, 5 months ago)

And you bumped this because?


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InvisibleFungus_monk
Here or there...
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Registered: 08/07/11
Posts: 1,589
Loc: U.S. Flag
Re: Neuron's first grow :) [Re: dtowntoker]
    #14957966 - 08/21/11 06:51 PM (12 years, 5 months ago)

You shouldnt be bumping within 24 hours, its a forum rule.


--------------------
My Trade List

I post fictional information that i have developed through years of extreme delirium and from suffering strange delusions that i partake in illegal activites. The shroomery.org is merely my outlet for these delusions.


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Offlinedon_vedo
MerKaBa
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Re: Neuron's first grow :) [Re: Fungus_monk]
    #14962942 - 08/22/11 07:34 PM (12 years, 5 months ago)

Looking good brother!

Lah'Kesh


--------------------
Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.


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Offlineneuron99
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Registered: 04/02/11
Posts: 95
Last seen: 5 years, 9 months
Re: Neuron's first grow :) [Re: don_vedo]
    #15032567 - 09/05/11 12:39 PM (12 years, 4 months ago)

thank you shroomery and all the wonderful members! i've been harvesting great flushes, i've even flipped the entire MSG cake from tub #1 over into a larger tub and fruiting a 4th flush like a gigantic PF cake with nice fruits up top and all around the sides

those that have tried the mushrooms say the potency is wonderful :thumbup: :dancer:


for some laughs



Edited by neuron99 (09/05/11 12:39 PM)


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OfflineShroom_i
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Re: Neuron's first grow :) [Re: neuron99]
    #15033684 - 09/05/11 04:33 PM (12 years, 4 months ago)

NICE!


--------------------
Prisoner#1 said:
[quote]destructo_low said:
Prisoner's posts by percentage.

Smart and witty - 9.9999922409044%

Kind and caring - 0.0000077590956%
[/quote]

worthy of a facepalm - 90%[/quote]


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InvisibleHorizonSpawn
Gettin' my grow on :)
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Registered: 05/14/11
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Loc: Myco-Tek.org Flag
Re: Neuron's first grow :) [Re: Shroom_i]
    #15034243 - 09/05/11 06:25 PM (12 years, 4 months ago)

Looks marvelous! :mushroom2:


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:


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