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sporophight
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Registered: 06/02/10
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Re: Neuron's first grow :) [Re: don_vedo]
#14584038 - 06/09/11 08:17 AM (12 years, 7 months ago) |
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Quote:
don_vedo said:
Quote:
sporophight said: First off, Great job being well informed and thorough. As far as shelving goes, If you are using a tub I would advise against making a sealed chamber as well. It is redundant and possibly detrimental. If you think that humidity fluctuations are going to be an issue then i would abandon the tub and use trays in a greenhouse/martha. Congrats on the new hobby! Good luck! 
I think neuron was going to use a msg type tub which is a monotub with small holes drilled in 5 sides kind of like a shotgun fc; he was going to use a sealed chamber to keep rh higher due to the fact that the msg might produce to much fae and dry the sub out in a normal room.
Lah'Kesh
I realize what a MSG is. I think that instead of combining completely different teks you should just adjust the number/size of holes. No single tub design will work in every environment. But with adjustments a tub can work in most.
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don_vedo
MerKaBa



Registered: 05/12/11
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Oh sorry thought you didn't realize it was an msg; I stated that, because after reading, I have found that doc T and several others have had great success with fruiting msg's in a gh or some sort of humidity tent.
Totally understand what your saying, definitely makes sense. I would imagine to each his own. Personally for me I am going to go with fruiting my msg's in a gh just cause the method has been proven to work and I don't have enough time in my daily life right now to experiment as much as id like.
Lah'Kesh
-------------------- Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.
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neuron99
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Re: Neuron's first grow :) [Re: don_vedo]
#14588144 - 06/09/11 10:34 PM (12 years, 7 months ago) |
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Yup I think I'm leaning towards a MSG in that shelf I posted up but setup like a GH with plastic lining, it would be semi-sealed
My glassblowing friend kindly donated me some material for my future LC jars Nice and sharp to break up the mycelium

And on order are some Whatman syringe filters for better LC lids, the Tyvek and polyfill designs make me concerned about wicking up contamination when I shake the jar
Should I check on the WBS jars periodically (1/week) or would it be better to just wait the 3 weeks? Does momentary ambient light exposure affect colonization at all?
Edited by neuron99 (06/09/11 10:44 PM)
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don_vedo
MerKaBa



Registered: 05/12/11
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Re: Neuron's first grow :) [Re: neuron99]
#14588201 - 06/09/11 10:46 PM (12 years, 7 months ago) |
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Nice buddy you got there!
I am doing some MSG tubs myself, how were you planning on doing your lighting with the shelves? Just not snapping the top shelf on? I think I am going to go with this greenhouse I found at Lowes.
http://www.lowes.com/pd_318839-10155-FHPT400CL_4294935924_4294937087_?productId=3111999&Ns=p_product_prd_lis_ord_nbr|0||p_product_quantity_sold|1&pl=1¤tURL=%2Fpl_Greenhouses_4294935924_4294937087_%3FNs%3Dp_product_prd_lis_ord_nbr|0||p_product_quantity_sold|1&facetInfo=
Just so I don't have to put one together myself, and i'm thinking it would end up being a good investment as I can use it for a long time, with several grows.
Lah'Kesh
-------------------- Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.
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neuron99
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Re: Neuron's first grow :) [Re: don_vedo]
#14588220 - 06/09/11 10:51 PM (12 years, 7 months ago) |
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Cool! That's the design I'll be trying to mimic, hopefully a DIY version will have significant savings with equal performance, if not I'll definitely ditch it for the Lowes one. I'm thinking of using black plastic for additional privacy.
As for lighting I was eyeing high output LED strip lighting or some low wattage under cabinet floro tubes (IIRC they come around ~12") each level would have it's own light. I'm leaning towards LED as long as it's not cost prohibitive
Edited by neuron99 (06/09/11 10:53 PM)
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don_vedo
MerKaBa



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Re: Neuron's first grow :) [Re: neuron99]
#14588315 - 06/09/11 11:15 PM (12 years, 7 months ago) |
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Good thinking on the lights, I think I am going to use a couple 6500k fluorescent bulbs, I have some lamps I was able to modify so that I can just hang them over the greenhouse itself and let the light shine in through the clear viynile.
Keep posting those pics man, love the step by step process! Watchin your every move 
Lah'Kesh
-------------------- Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.
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FuckMeRunnin
REALLY good looking


Registered: 07/18/09
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Re: Neuron's first grow :) [Re: don_vedo]
#14588351 - 06/09/11 11:24 PM (12 years, 7 months ago) |
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What I don't understand, is it seems that you made a hella over the top glove box(very cool by the way). Yet your jars only are protected by coffee filters on top, no micopore tape, tyvek, or polyfill. I always thought coffee filters were more of an extra not a main. I always follow docs wbs tek to the max, by adding polyfill to the inoculation points, tyvek over that, and coffee filter after i poke a hole through the tyvek. I'm sure It's just me being super anal, I'm just confused at how you seem to have spent mad time on the glove box(mine is just a box with 2 holes haha) yet not much extra time on adding as much protection to the injection points as possible. I dunno you could of added tape after inoculating, I couldn't tell. Good luck buddy.
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Teemo
Swift Scout



Registered: 01/09/11
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Quote:
FuckMeRunnin said: What I don't understand, is it seems that you made a hella over the top glove box(very cool by the way). Yet your jars only are protected by coffee filters on top, no micopore tape, tyvek, or polyfill. I always thought coffee filters were more of an extra not a main. I always follow docs wbs tek to the max, by adding polyfill to the inoculation points, tyvek over that, and coffee filter after i poke a hole through the tyvek. I'm sure It's just me being super anal, I'm just confused at how you seem to have spent mad time on the glove box(mine is just a box with 2 holes haha) yet not much extra time on adding as much protection to the injection points as possible. I dunno you could of added tape after inoculating, I couldn't tell. Good luck buddy.
I think he used silicone injection holes so he wouldn't need polyfill.. also I've heard that tyvek + polyfill + coffee filters restricts FAE which is still important in colonizing myc. I know I've posted pics of my jars a few times here in cult (I do polyfill + coffee filters) and I've been scolded and told to take off the coffee filters, since they really wouldn't block any trich or other mold spores, and hinder my efforts by restricting FAE. (I usually leave my coffee filters on until they're mostly colonized though, just cuz I've got a dusty house)
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"How terribly sad it was that people are made in such a way that they get used to something as extraordinary as living."
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FuckMeRunnin
REALLY good looking


Registered: 07/18/09
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I thought that he added that stuff to the lc jars not the grain. I could be wrong, and wouldn't self healing silicon holes prevent FAE more so then 3 layers of material? The reason I say this is because the LC tek i followed called for the back of a syringe filled with polyfill, next to the self healing injection point. This way you wouldn't have to manually pump air into the jar with a needle every so often.
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neuron99
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Registered: 04/02/11
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The WBS jars have Tyvek + micropore tape over injection site in tyvek, and double coffee filter
The 1st version LC lids had the silicone port but I dumped the LC jars pictured and am modifying the lids to also include Whatman syring filter for GE so I don't have to depressurize with an amply sterilized syringe
Edited by neuron99 (06/10/11 01:09 AM)
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FuckMeRunnin
REALLY good looking


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Re: Neuron's first grow :) [Re: neuron99]
#14588537 - 06/10/11 12:23 AM (12 years, 7 months ago) |
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Yes, the syringe filter idea was genius, I have used it many times and never had a problem...Hell I forgot about one over a year and a half ago, I recently inoculated jars and low and behold no contams :-) ps why didn't you just keep the tyvek on, under the jar ring? Then again with the box you have going there's nothing getting in haha.
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neuron99
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the Tyvek is placed over the glass mason jar, under the lid w/ holes, both under the screw top collar
Edited by neuron99 (06/10/11 01:11 AM)
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neuron99
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Re: Neuron's first grow :) [Re: neuron99]
#14623554 - 06/16/11 03:57 PM (12 years, 7 months ago) |
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It's been about a week so I took a peek to see how things are going, the Tx seem to have started colonizing, I can see faint patches of whitish hairs formimg, the Lip and Eq don't show any signs yet.
Some goodies arrived in the mail:


Still waiting on the Whatman filters, once they get in it's time to whip up some LC jars
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don_vedo
MerKaBa



Registered: 05/12/11
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Re: Neuron's first grow :) [Re: neuron99]
#14623629 - 06/16/11 04:08 PM (12 years, 7 months ago) |
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Looks good neuron, I normally do bags but usually once I start seeing growth they take off from there and don't look back. My current grow, the bags showed signs in 7 days and then 7 days later they were 100%. I spawned to bulk Tuesday night so I'll give them a week until I take a peek. My point being that they should start rolling now for you! Good luck and good vibes brother, keep us posted on your LC.
Lah'Kesh
-------------------- Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.
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neuron99
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Re: Neuron's first grow :) [Re: don_vedo]
#14642953 - 06/20/11 12:56 PM (12 years, 7 months ago) |
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Been checking up on the jars periodically, they have all started to colonize, Tx being much faster than the other two.
unfortunately I think I've spotted a contamination in one of my Tx jars, can someone confirm:


should I toss the jar? or dump it outdoors somewhere
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jpshroomer
hUnTsMaN



Registered: 05/09/11
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Re: Neuron's first grow :) [Re: neuron99]
#14643007 - 06/20/11 01:06 PM (12 years, 7 months ago) |
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it looks like trich to me. def toss it or put outside see what happens but get it away from the others...
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neuron99
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Re: Neuron's first grow :) [Re: jpshroomer]
#14643177 - 06/20/11 01:33 PM (12 years, 7 months ago) |
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damn, I found another jar with a tiny green speck. makes that 2/12 jars, both Texans.
I feel like a dirty person lol contaminated even with a glovebox
On the plus, the others are nice and healthy white. But only about 5% colonized (generous estimate). Will need to check end of the month.
Edited by neuron99 (06/20/11 01:34 PM)
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don_vedo
MerKaBa



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Re: Neuron's first grow :) [Re: neuron99]
#14643704 - 06/20/11 03:16 PM (12 years, 7 months ago) |
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Yah I would definitely say trich and would say to get them away from your grow area ASAP brother, no reason to get the those spores all over the place! Personally would toss the jars but I guess you could throw them outdoors and see why happens.
Lah'Kesh
-------------------- Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.
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neuron99
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Re: Neuron's first grow :) [Re: don_vedo]
#14710840 - 07/03/11 12:32 PM (12 years, 6 months ago) |
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Here's a quick update:



I'm a bit concerned about this jar, it seems to have quite a bit of moisture but I don't see anything discolored. All the growth seems to be bright white.

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Gzaajhom
title


Registered: 12/11/10
Posts: 183
Loc: USA
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Re: Neuron's first grow :) [Re: neuron99]
#14711119 - 07/03/11 01:41 PM (12 years, 6 months ago) |
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It looks like the last one has a bacterial infection. The 7x may also have a contaminant but its hard to tell. Bacteria makes the grains look wet/slimy, and when you shake it up the grains that were sticking to the glass will leave a brown residue.
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Don't hate the player; hate the game. -Abraham Lincoln
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