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neuron99
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Registered: 04/02/11
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Last seen: 5 years, 9 months
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Re: Neuron's first grow :) [Re: don_vedo]
#14574848 - 06/07/11 02:44 PM (12 years, 7 months ago) |
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Quote:
don_vedo said:
Quote:
neuron99 said: Yeah the PVC piping and sheeting is a good idea, I figured the shelving is on sale at Walmart for $14 and is likely to be structurally more rigid than something I put together. I was debating about Black/White poly for privacy purposes but regular plastic sheeting would definitely be my second best alternative and equally effective.
Thanks for the info on the shelves brother, I think im going to pick one up myself and just throw the plastic sheeting over it. Then throw my humidifier on the bottom shelf. Seems pretty easy, and a great way to keep rh high without worrying about it dropping off when I am at work.
Lah'Kesh
You're very welcome! As a former cannabis cultivator my eyes are trained to scope out makeshift/McGuyver potential grow boxes (i.e. shelving units & storage cabinets)
WBS has been rinsed quite thoroughly and strained to dry after a 14 hour soak. Having (2) 5 gallon buckets helped alot during the rinse.

Just finished drilling 5/16" holes into the quart jars.

Off to go fill my jars! I'm straining it one more time just in case...I think I did a fine job initially as I'm not seeing any more residual water form
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don_vedo
MerKaBa



Registered: 05/12/11
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Re: Neuron's first grow :) [Re: neuron99]
#14575159 - 06/07/11 03:47 PM (12 years, 7 months ago) |
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Looks great man, I love the updates. You must have been a great cannabis grower, I can tell you've definitely done your research thus far. I see alot of people coming here and just asking questions instead of taking the time to read through and find there own answers.
Lah'Kesh
-------------------- Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.
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neuron99
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Registered: 04/02/11
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Re: Neuron's first grow :) [Re: don_vedo]
#14575979 - 06/07/11 06:42 PM (12 years, 7 months ago) |
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Quote:
don_vedo said: Looks great man, I love the updates. You must have been a great cannabis grower, I can tell you've definitely done your research thus far. I see alot of people coming here and just asking questions instead of taking the time to read through and find there own answers.
Lah'Kesh
Thanks! I try to use the search engine when I can lol
So here's where I'm at now.
The pressure cooker can hold a maximum of 7 quart jars so I decided to process these jars in batches of 6:


While I wait for the first batch to complete, I started making some self-healing lids for future honey LC use. Cutting holes out of a cardboard box helps with the drying of RTV silicone without touching any other surfaces:
These lids lack GE, see updated post for better design.


Looks like I have way more WBS than I need, I ended up filling additional 6 quarts (as planned) and another 6 pints (unplanned).
I'm still undecided what to do with the pint jars, I may use 3 of em and try a Grain LC. Not sure what to do with the other 3.

I pressure cooked them for 90 minutes, while many people have PC'd for 60 minutes I figured the extra 30 minutes may be beneficial for me as I opted out of simmering the WBS initially.

After a rigorous, sweat-inducing shake, straight into the oven for cooling

Edited by neuron99 (08/17/11 03:34 PM)
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neuron99
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Re: Neuron's first grow :) [Re: neuron99]
#14576589 - 06/07/11 08:42 PM (12 years, 7 months ago) |
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So I decided to not get ahead of myself and dumped out the pint jars. I'm going to stick with the original plan of 2ml of MS directly inoculated into each 1QT WBS jar, using a total of 24ml of the 30ml spore solution that I have. With the remaining 6ml (2ml each of 3 races) I will attempt to make honey LC in pint jars using 1ml, saving the last 1ml just in case my first attempt doesn't go well.
A days work done, 2 rounds of PC'ing, tomorrow morning is going to be exciting! Inoculation time! Get to use my glove box!

I'm not sure if I can stand to hear another couple hours of the PC's rattling, nor is the RTV completely set on the self-healing LC lids, so I'll be whipping up my honey LC and PC'ing it tmrw morning.
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neuron99
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Re: Neuron's first grow :) [Re: neuron99]
#14578927 - 06/08/11 09:51 AM (12 years, 7 months ago) |
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Good mornin shoomery!
Today is inoculation day!
But first, will start brewing the honey LC as the RTV lids are finally finished (I'll be making the LC with the remaining solution after inoculating MS into WBS): See later posts for better LC lid design, ditched this one
Edited by neuron99 (08/17/11 03:33 PM)
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don_vedo
MerKaBa



Registered: 05/12/11
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Re: Neuron's first grow :) [Re: neuron99]
#14578950 - 06/08/11 09:59 AM (12 years, 7 months ago) |
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Nice keep up the updates brother, good vibes and good luck 
Lah'Kesh
-------------------- Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.
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Loriath
Needing Practice

Registered: 07/22/05
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Loc: Canada
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Re: Neuron's first grow :) [Re: don_vedo]
#14579046 - 06/08/11 10:24 AM (12 years, 7 months ago) |
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Im really excited about this grow, you have done tons of research and for someone new at it you seem to have every aspect covered!
cant wait to watch those jars colonize.
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neuron99
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Re: Neuron's first grow :) [Re: Loriath]
#14579067 - 06/08/11 10:31 AM (12 years, 7 months ago) |
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Okay so I whipped up the honey LC, a 4% solution so in these pint jars I used about ~10 ounces/~300 ml of water and ~2.5 teaspoons/~12.5 ml of clover honey.
Gives me a few inches of space for shaking room as I don't have any broken glass or marbles on hand ATM (I had a whole box of broken borosilicate last month, little did I know I would have future use for it lol)
Anyways, into the PC they go! I'm using the single port RTV design lid so the mason jars have been tightened and then loosened 1/4 turn.

As a reminder to myself, I'm gonna need to use the empty syringe+iso soaked poly method to de-pressurize before inoculating. Planning on 1cc in each jar.
Cleaned out an empty cabinet to prepare for storage. Man, this is a good hobby to have for a stoner, I don't think I've cleaned my house like this ever before.
The cabinet, completely empty, is at a constant 80*F. IIRC, the jars themselves will be producing some heat, I hope that this environment is ideal.

Cooled down WBS jars now patiently awaiting inoculation, I gotta go run an errand soon, gonna wait until the afternoon when I have a nice chunk of time so nothings rushed.
Edited by neuron99 (08/17/11 03:35 PM)
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sporophight
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Re: Neuron's first grow :) [Re: neuron99]
#14579185 - 06/08/11 10:59 AM (12 years, 7 months ago) |
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First off, Great job being well informed and thorough. As far as shelving goes, If you are using a tub I would advise against making a sealed chamber as well. It is redundant and possibly detrimental. If you think that humidity fluctuations are going to be an issue then i would abandon the tub and use trays in a greenhouse/martha. Congrats on the new hobby! Good luck!
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don_vedo
MerKaBa



Registered: 05/12/11
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Quote:
sporophight said: First off, Great job being well informed and thorough. As far as shelving goes, If you are using a tub I would advise against making a sealed chamber as well. It is redundant and possibly detrimental. If you think that humidity fluctuations are going to be an issue then i would abandon the tub and use trays in a greenhouse/martha. Congrats on the new hobby! Good luck! 
I think neuron was going to use a msg type tub which is a monotub with small holes drilled in 5 sides kind of like a shotgun fc; he was going to use a sealed chamber to keep rh higher due to the fact that the msg might produce to much fae and dry the sub out in a normal room.
Lah'Kesh
-------------------- Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.
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higgledy-piggledy
Registered: 08/24/10
Posts: 966
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Re: Neuron's first grow :) [Re: neuron99]
#14579297 - 06/08/11 11:28 AM (12 years, 7 months ago) |
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Quote:
neuron99 said: ... I hope that this environment is ideal.
with the doors of your cabinet open your jars get nice ambient light.
you also could dismantle them...
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neuron99
Stranger


Registered: 04/02/11
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Quote:
higgledy-piggledy said:
Quote:
neuron99 said: ... I hope that this environment is ideal.
with the doors of your cabinet open your jars get nice ambient light.
you also could dismantle them...
for colonization do I need ambient light? I was planning on closing the cabinet and forgetting about it for a couple weeks to allow for colonization
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don_vedo
MerKaBa



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Re: Neuron's first grow :) [Re: neuron99]
#14579408 - 06/08/11 11:52 AM (12 years, 7 months ago) |
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Quote:
neuron99 said: for colonization do I need ambient light? I was planning on closing the cabinet and forgetting about it for a couple weeks to allow for colonization
I have bags going great right now with no ambient light, only light they get is from me checking them every once in a while. I think it could go either way.
Lah'Kesh
-------------------- Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.
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neuron99
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Re: Neuron's first grow :) [Re: don_vedo]
#14579425 - 06/08/11 11:57 AM (12 years, 7 months ago) |
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okay cool I was under the impression that darkness is ideal for colonization and ambient light should be introduced to induce pinning
I don't plan on fruiting in that cabinet, first the space is way too limited for the 12 qts of spawn I'm creating, additionally it's above my fridge and sort of a PITA to access. Good for privacy, not for when things get bulky.
I just finished PC'ing the honey water for 15 minutes @ 15psi, however I'm afraid that I overcooked the honey. Is this what it's supposed to look like? I don't recall seeing so much sediment in others, while I have read a couple members post saying they coffee filter their honey water. Am I going to be okay inoculating these as soon as they cool down?


Edited by neuron99 (06/08/11 12:01 PM)
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neuron99
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Re: Neuron's first grow :) [Re: neuron99]
#14580396 - 06/08/11 03:25 PM (12 years, 7 months ago) |
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Inoculated 2cc into each 1qt jar of WBS:








(not pictured: wiping of hot needle w/ alcohol soaked paper towel)




After a quick shake, back into the cabinet they go:

See you in 2~3 weeks!
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don_vedo
MerKaBa



Registered: 05/12/11
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Re: Neuron's first grow :) [Re: neuron99]
#14580427 - 06/08/11 03:31 PM (12 years, 7 months ago) |
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Nice job brother, good vibes comin your way 
Can't wait to see the progress!
Lah'kesh
-------------------- Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.
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k00laid
NEMO


Registered: 05/03/10
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Re: Neuron's first grow :) [Re: don_vedo]
#14581259 - 06/08/11 06:40 PM (12 years, 7 months ago) |
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you sanitized a sterilized needle.
you went backwards >.>
probs wont hurt
but to cool needles. you expel a drop of spore solution. dont touch your needle with anything.
-------------------- AMU - AMU Q & A - MyVideo Teks!
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neuron99
Stranger


Registered: 04/02/11
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Re: Neuron's first grow :) [Re: k00laid]
#14581758 - 06/08/11 08:35 PM (12 years, 7 months ago) |
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I was following Doc's WBS tek, specifically the inoculation steps where he advises flame sterilization followed by alcohol wipe before inoculating the jar and then another alcohol wipe after injection
But either way, thanks for the heads up, I don't think one can be too sterile
Edited by neuron99 (06/08/11 08:36 PM)
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k00laid
NEMO


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Re: Neuron's first grow :) [Re: neuron99]
#14581781 - 06/08/11 08:39 PM (12 years, 7 months ago) |
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well. once you learn the difference between sanitizing and sterilizing you will know why you wiping with alcohol can actually be detrimental.
but im just being nit picky. people do it that way all the time.
xD
-------------------- AMU - AMU Q & A - MyVideo Teks!
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Selfindulgence
Funk Master



Registered: 06/01/11
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Re: Neuron's first grow :) [Re: k00laid]
#14581982 - 06/08/11 09:14 PM (12 years, 7 months ago) |
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I realize it seems to be echoing through this thread but amazing first grow. You've inspired me to change my entire approach on my next grow. Awesome attention to detail brother.
-------------------- Fuck L Ron Hubbard and Fuck all his clones. Fuck all those gun-toting Hip gangster wannabes. Fuck retro anything. Fuck your tattoos. Fuck all you junkies and Fuck your short memory. Fuck smiley glad-hands With hidden agendas. Fuck these dysfunctional, Insecure actresses. Learn to swim. Cuz I'm praying for rain and I'm praying for tidal waves I wanna see the ground give way. I wanna watch it all go down. Mom please flush it all away. I wanna watch it go right in and down. I wanna watch it go right in. Watch you flush it all away. Time to bring it down again. Don't just call me pessimist. Try and read between the lines. I can't imagine why you wouldn't Welcome any change, my friend. I wanna see it all come down. suck it down. flush it down.
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