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neuron99
Stranger


Registered: 04/02/11
Posts: 95
Last seen: 5 years, 10 months
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~=.Neuron's grow. [w/ PIX!]=~ 1
#14564722 - 06/05/11 01:39 PM (12 years, 8 months ago) |
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Hello everybody! Coming from the world of cannabis, this is my first time growing psilocybin.
I've lurked these forums and have already soaked up so much information, it's exciting to embark on this journey with the help of such a knowledgeably community.
I ordered spores but forgot which strains I chose... I do know the order code which was the following:
> eq2: 1 > > Lipa1: 1 > > Tx2: 1
so whatever those may be (if anyone can recognize the shorthand plz let me know!), I'm just excited to have spores and begin!
special thanks to member "realfuzzhead" for lending a helping hand!
here's the game plan--not that you guys need to know--but more for myself, as I keep forgetting the stages and specifics lol
1.) purchase cubensis, WBS, pressure cooker, latex gloves, mason jars, silicone, tyvek , 99% isopropyl alcohol and power drill 2.) prepare liquid culture, prepare WBS 3.) inject WBS with LC, wait and let jars colonize 4.) spawn WBS to pasturized coir, let it colonize 5.) place tray in fruiting chamber 6.) harvest and dry
I've decided on the following teks:
-Cyber's LC Tek (opting to use honey) -Cyber's self-healing lids -6T WBS Tek -Damion5050's Coir Tek -Monotub
My first trip to Walmart, I need to go back b/c the pressure cooker is missing a part Clear tub is to build a glovebox for myself, I still need to go get some PVC couplings and glue.

Since I have to go back to Walmart I decided might as well verify with you guys if I got the suitable items
I want to verify that this is the best type of polyfill.... I noticed many kinds, including higher density premium version as well as quilted versions

Also, this is the WBS I have chosen:

This was the only honey at Walmart, will this work? There are organic stores nearby (whole foods/bristol farms) that I can go to if it really makes a difference, I do want to do everything right the first time.
Also, is the proper RTV? I noticed some posts ppl were using the stronger grey color:

Thanks!!!!
Edited by neuron99 (09/05/11 01:06 PM)
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DoubleG909



Registered: 05/08/11
Posts: 136
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Re: Neuron's first grow :) [Re: neuron99]
#14564755 - 06/05/11 01:51 PM (12 years, 8 months ago) |
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Can you return your blue rtv and get the red tube? The red is high-temp and will withstand the PC. I see you have polyfill and wbs so I'm guessing your jumping right into bulk with a monotub? If so you may wanna get some quart sized mason jars. I like that your making a glove box right from the get go!
-------------------- “Nature is not our enemy, to be raped and conquered. Nature is ourselves, to be cherished and explored.” ― Terence McKenna
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neuron99
Stranger


Registered: 04/02/11
Posts: 95
Last seen: 5 years, 10 months
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Re: Neuron's first grow :) [Re: DoubleG909]
#14564775 - 06/05/11 01:59 PM (12 years, 8 months ago) |
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Quote:
DoubleG909 said: Can you return your blue rtv and get the red tube? The red is high-temp and will withstand the PC. I see you have polyfill and wbs so I'm guessing your jumping right into bulk with a monotub? If so you may wanna get some quart sized mason jars. I like that your making a glove box right from the get go!
I was looking specifically for the red type but unfortunately they didn't have any. The temperature range for the blue was -65*F to 500*F, the other grey one IIRC had the same temp range but had less flexibility. I was thinking more flexibility would translate into more "pokes" before I had to re-silicone the injection port
I am however going to a different Walmart today so I'm bringing the RTV with me and snagging the highest temp gasket-maker silicone
Yeah the pint jars are for the honey LC, I will be purchasing quart jars for the bulk
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DoubleG909



Registered: 05/08/11
Posts: 136
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Re: Neuron's first grow :) [Re: DoubleG909]
#14564776 - 06/05/11 02:00 PM (12 years, 8 months ago) |
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If you don't want to wait for your LC to fully colonize you can do your first batch of jars multispore. Use like 1 or 2 cc's from the syringe into your LC and the rest on your WBS.
-------------------- “Nature is not our enemy, to be raped and conquered. Nature is ourselves, to be cherished and explored.” ― Terence McKenna
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neuron99
Stranger


Registered: 04/02/11
Posts: 95
Last seen: 5 years, 10 months
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Re: Neuron's first grow :) [Re: DoubleG909]
#14564798 - 06/05/11 02:06 PM (12 years, 8 months ago) |
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Oh that's a good idea I didn't think of that
Maybe I'll use 6cc of the 10cc of each strain, while reserving 4cc to make LC (enough for two attempts @2cc ea. in case the first one fails to colonize)
So using 6cc, that's 3 quart jars? Gotta start crunching numbers to figure out which size monotub to get
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neuron99
Stranger


Registered: 04/02/11
Posts: 95
Last seen: 5 years, 10 months
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Re: Neuron's first grow :) [Re: neuron99]
#14566705 - 06/05/11 09:43 PM (12 years, 8 months ago) |
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Okay back from Walmart and Home Depot with a fresh new pressure cooker and some new goodies! 
Quart jars for WBS Plastic wide-mouth lids Red RTV Silicone Toilet flanges for glovebox Hoseclamps to secure gloves Drill bit kit Epoxy (Not pictured: stack of USPS Express mail Tyvek)
Edited by neuron99 (06/05/11 10:16 PM)
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
Last seen: 7 months, 6 days
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Re: Neuron's first grow :) [Re: neuron99]
#14566718 - 06/05/11 09:46 PM (12 years, 8 months ago) |
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i dont even use rtv silicone.
i use the bathroom stuff.
PCs just fine
-------------------- AMU - AMU Q & A - MyVideo Teks!
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Shroom_i
Gatherer




Registered: 10/13/10
Posts: 868
Loc: Zone Six (6)
Last seen: 7 years, 1 month
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Re: Neuron's first grow :) [Re: k00laid]
#14566777 - 06/05/11 09:59 PM (12 years, 8 months ago) |
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Lookin good to me, I'll be following this.
-------------------- Prisoner#1 said: [quote]destructo_low said: Prisoner's posts by percentage. Smart and witty - 9.9999922409044% Kind and caring - 0.0000077590956% [/quote] worthy of a facepalm - 90%[/quote]
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higgledy-piggledy
Registered: 08/24/10
Posts: 966
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Re: Neuron's first grow :) [Re: Shroom_i]
#14567055 - 06/05/11 10:45 PM (12 years, 8 months ago) |
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you are prepared.
(one of the very few people)
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Gzaajhom
title


Registered: 12/11/10
Posts: 183
Loc: USA
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Re: Neuron's first grow :) [Re: Shroom_i]
#14567059 - 06/05/11 10:46 PM (12 years, 8 months ago) |
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Looks good. I would suggest making a myc syringe instead of an LC though. Basically, what you do is inoculate a small amount of grains (about an inch in the quart jars), let it colonize, and then shoot water into the jar, shake, and draw the myc water back up into the syringe to inoculate other jars. That way, you can know for sure if there are any contaminants. It can be difficult to tell with LC.
Heres a pictorial from agar: GRAIN LC
LC's are still good though and will probably work fine anyway.
--------------------
Don't hate the player; hate the game. -Abraham Lincoln
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HorizonSpawn
Gettin' my grow on :)


Registered: 05/14/11
Posts: 1,739
Loc: Myco-Tek.org
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Re: Neuron's first grow :) [Re: Gzaajhom]
#14567130 - 06/05/11 10:56 PM (12 years, 8 months ago) |
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Hmmmmm... If you plan on using the plastic lids, you might want to consider acquiring gaskets for them... Just a thought... I'm a total noob...
Oh... Almost forgot... I don't think I've ever seen a silicone-based glue rated lower than 400degF... You'll only be pressurizing to about 250degF...
-------------------- Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC... NOTE: Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone
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don_vedo
MerKaBa



Registered: 05/12/11
Posts: 1,383
Loc: 5th dimension
Last seen: 3 years, 10 months
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Looks like your set up and getting ready to go! Happy your here brother, i'll definitely be following this thread; good vibes
Lah'Kesh
-------------------- Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.
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Shroom_i
Gatherer




Registered: 10/13/10
Posts: 868
Loc: Zone Six (6)
Last seen: 7 years, 1 month
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Re: Neuron's first grow :) [Re: neuron99]
#14567358 - 06/05/11 11:39 PM (12 years, 8 months ago) |
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Oh I noticed that no one gae you the "strain" names.
Equador Thai Lipa Texas
Here's a good link about "strains" and why that word is misinforming.
Might as well get it now while you're new.
http://www.shroomery.org/forums/showflat.php/Number/7955834
-------------------- Prisoner#1 said: [quote]destructo_low said: Prisoner's posts by percentage. Smart and witty - 9.9999922409044% Kind and caring - 0.0000077590956% [/quote] worthy of a facepalm - 90%[/quote]
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neuron99
Stranger


Registered: 04/02/11
Posts: 95
Last seen: 5 years, 10 months
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Re: Neuron's first grow :) [Re: Shroom_i]
#14567720 - 06/06/11 01:35 AM (12 years, 8 months ago) |
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Quote:
Shroom_i said: Lookin good to me, I'll be following this.
Quote:
higgledy-piggledy said:

you are prepared.
(one of the very few people)
thanks! i'm a forum addict and am equally excited for all the learning to do and ppl to converse with as I am for my first harvest
Quote:
HorizonSpawn said: Hmmmmm... If you plan on using the plastic lids, you might want to consider acquiring gaskets for them... Just a thought... I'm a total noob...
Oh... Almost forgot... I don't think I've ever seen a silicone-based glue rated lower than 400degF... You'll only be pressurizing to about 250degF...
I have both metal and plastic lids ATM and haven't drilled out anything yet, gonna do some more reading before settling on a lid design I like
Quote:
Gzaajhom said: Looks good. I would suggest making a myc syringe instead of an LC though. Basically, what you do is inoculate a small amount of grains (about an inch in the quart jars), let it colonize, and then shoot water into the jar, shake, and draw the myc water back up into the syringe to inoculate other jars. That way, you can know for sure if there are any contaminants. It can be difficult to tell with LC.
Heres a pictorial from agar: GRAIN LC
LC's are still good though and will probably work fine anyway.
Unfortunately the link doesn't work for me, or I don't have permission to read it yet. I'll have to take a look into this!
Quote:
don_vedo said: Looks like your set up and getting ready to go! Happy your here brother, i'll definitely be following this thread; good vibes
Lah'Kesh
Quote:
Shroom_i said: Oh I noticed that no one gae you the "strain" names.
Equador Thai Lipa Texas
Here's a good link about "strains" and why that word is misinforming.
Might as well get it now while you're new.
http://www.shroomery.org/forums/showflat.php/Number/7955834
thank you thank you!!
So here's where I'm at, just finished building most of my glovebox, waiting for the silicone to set. All that's left is to hoseclamp the gloves onto the couplings.

Complete with warning sticker making sure I put no babies in my glovebox 

I put a Tyvek vent, not sure if this is essential or not, I did a quick forum search and decided on my own that it wouldn't hurt, esp with flame sterilization and alcohol being in the same container and the gloves themselves not being Tyvek but rubber instead.
Edited by neuron99 (06/06/11 01:43 AM)
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neuron99
Stranger


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Re: Neuron's first grow :) [Re: neuron99]
#14571528 - 06/06/11 09:04 PM (12 years, 8 months ago) |
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okay, the silicone is set and glove box is finally complete:

and WBS currently being soaked in tap water to fill 9 qt jars (3 jars @3qt x 3 races)
I'm following the 6T tek (http://www.shroomery.org/9032/6T-WBS-Tek-Wild-Bird-Seed-Bulk-Spawn-Preparation-Method)which simply says to soak and that floaters don't matter
however I've come across Doc's tek (http://www.shroomery.org/9030/Docs-Wild-Bird-Seed-Tek) which is much more involved and involves removing floaters as well as repeated washing and simmering. I don't mind the additional labor if it will make a significant difference just curious to see what others think.

I've decided to directly inoculate the WBS with 6ml of the 10ml that I have (the 6ml split 2ml/qt) while saving the remaining 4ml to produce Honey LC for future use
Unfortunately my syringes are not marked in any matter so I will have to call them "1" "2" and "3" and visually identify them after fruiting
On order: -12ml Luer Lock Syringe no needle 100box -18 gauge needles x 100 (way more than I need lol)
I was eying some Sterilite 27qt boxes today, they are nice and clear with semi-clear tops. I'm thinking of using them in conjunction with black trash bags for the final couple stages. (3) 27qt boxes with 3qt of spawn in each, mixed with coco and coffee (ratios still undetermined)
Edited by neuron99 (06/06/11 09:27 PM)
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don_vedo
MerKaBa



Registered: 05/12/11
Posts: 1,383
Loc: 5th dimension
Last seen: 3 years, 10 months
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Re: Neuron's first grow :) [Re: neuron99]
#14571968 - 06/06/11 10:35 PM (12 years, 8 months ago) |
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Looks like you have put in a lot of time reading, that is definitely always good! Glovebox looks high tech, nice work with it man. From my experiences Doc's got some great TEK's, I personally have gone with his MSG for fruiting, couldn't say anything bad about it. I'm sure you'll have fantastic flushes! Good Vibes brother !
Lah'Kesh
-------------------- Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.
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neuron99
Stranger


Registered: 04/02/11
Posts: 95
Last seen: 5 years, 10 months
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Re: Neuron's first grow :) [Re: don_vedo]
#14572545 - 06/07/11 01:21 AM (12 years, 8 months ago) |
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just smoking some bho waiting for those WBS to soak...
I don't think I'm going to do the simmering but will give a good rinsing after a 12 hour soak, if anyone has objections please do chime in.
I'm starting to look ahead and plan the colonizing and fruiting stages. I have a space in the closet I plan on using, I'm thinking of buying a plastic storage shelf unit and wrap with black/white poly film for the colonizing process and then place LED strip lighting underneath each shelf for lighting during fruiting. Each shelf will have a MSG.
A cheaper alternative would be to use black trashbags for colonization and then use a clear plastic covering for the shelves during fruiting (for consistent humidity and addition barrier to contamination)
If I do make a somewhat sealed environment during fruiting, do I need to create some ventilation system for FAE? or would the air contained within be enough to circulate? they will be in MSG it just seems from what I've read a consistent RH is necessary for successful use
Edited by neuron99 (06/07/11 01:34 AM)
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Teemo
Swift Scout



Registered: 01/09/11
Posts: 192
Loc: Lost at sea
Last seen: 12 years, 7 months
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Re: Neuron's first grow :) [Re: neuron99]
#14572571 - 06/07/11 01:36 AM (12 years, 8 months ago) |
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Great job preparing and planning, Neuron! 
I don't think I can be of much help, but I know that according to Doc's WBS tek, simmering helps the seed to absorb more moisture... and it works well for me. I'm not sure how big of a difference simmering vs. not simmering makes, so hopefully someone else will chime in on that.
High humidity is important, but fresh air exchange is equally so. It seems to me that if you were to use a plastic storage shelf, that would just create a lot of stale air, so you would definitely need a ventilation setup. Although there'd be a lot of air in it, there'd be no air going out and no fresh air coming in.
Might I suggest you build a greenhouse in your closet with PVC pipe and plastic sheeting instead? Or perhaps a martha? You're doing trays, right? Those would provide both the controlled humidity and FAE you want.
Good luck!
--------------------
"How terribly sad it was that people are made in such a way that they get used to something as extraordinary as living."
Edited by Teemo (06/07/11 01:38 AM)
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neuron99
Stranger


Registered: 04/02/11
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Last seen: 5 years, 10 months
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Re: Neuron's first grow :) [Re: Teemo]
#14572600 - 06/07/11 01:50 AM (12 years, 8 months ago) |
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Thanks!
I was planning on using DocT's hybrid MSG, specifically the Sterilite tubs that are made for file hanging.
After reading, I think this is a good way to divide my risk and I think I'll be able to fit two tubs per shelf, each with 2 qts spawn. (So instead of the 9qts I was originally planning I may have to up it to 12 qts...I soaked 10 dry qts so hopefully with the 20-25% expansion and 3/4 fill I'll get 12).
The MSG seem to be designed such that they can be used without additional housing but I also did read a member saying fluctuations in RH were quite detrimental to his grow, which is why I considered enclosing MSG within a humidity tent as advised. Im just not sure what the essential features of a humidity tent are.
Yeah the PVC piping and sheeting is a good idea, I figured the shelving is on sale at Walmart for $14 and is likely to be structurally more rigid than something I put together. I was debating about Black/White poly for privacy purposes but regular plastic sheeting would definitely be my second best alternative and equally effective.
Edited by neuron99 (06/07/11 03:47 AM)
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don_vedo
MerKaBa



Registered: 05/12/11
Posts: 1,383
Loc: 5th dimension
Last seen: 3 years, 10 months
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Re: Neuron's first grow :) [Re: neuron99]
#14574070 - 06/07/11 11:53 AM (12 years, 8 months ago) |
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Quote:
neuron99 said: Yeah the PVC piping and sheeting is a good idea, I figured the shelving is on sale at Walmart for $14 and is likely to be structurally more rigid than something I put together. I was debating about Black/White poly for privacy purposes but regular plastic sheeting would definitely be my second best alternative and equally effective.
Thanks for the info on the shelves brother, I think im going to pick one up myself and just throw the plastic sheeting over it. Then throw my humidifier on the bottom shelf. Seems pretty easy, and a great way to keep rh high without worrying about it dropping off when I am at work.
Lah'Kesh
-------------------- Our deepest fear is not that we are inadequate. Our deepest fear is that we are powerful beyond measure. We ask ourselves, Who am I to be brilliant, gorgeous, talented, fabulous? Actually, who are you not to be? We were born to make manifest the glory of God that is within us all. And as we let our own light shine, we unconsciously give other people permission to do the same.
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