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Offlineexplosivekush
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contam porn! [ 2nd try, all contams again.. ] with pics
    #14564121 - 06/05/11 10:53 AM (12 years, 8 months ago)

i think it is my syringe because the contams follows the trail like the spore water and there is only contamination around all inoc spots.
these are my own made sterilized jars, with the same inoculant


Edited by explosivekush (02/10/13 07:13 PM)

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OfflineNature Boy
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14564149 - 06/05/11 11:00 AM (12 years, 8 months ago)

Doesn't matter what is in the jar component/ingredient wise at this point.  What IS important is that you recognized that there is trich.  DO NOT open the jars.  Discard as soon as possible!!!!!!!

By the way, its W-I-P-E-D.  Whipped is what you do to cream, or chicks into S & M.  :lol:

N.B.


--------------------
All submitted posts under this user name are works of pure fiction or outright lies.  Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit.  Note well:  Sorry, but I do not answer PM's unless you are a long-time trusted friend.  If you have a question, ask it in the appropriate thread.

                                                                               

Edited by Nature Boy (06/05/11 11:02 AM)

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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Nature Boy]
    #14564171 - 06/05/11 11:05 AM (12 years, 8 months ago)

haha yea i fixed that lol

on one of my jars actually i noticed some mycelium (  :Awemush: )

but on the other side of the jar.. lots of yellow / green dots. I think ima wait on that jar and see who wins the war d: probably discard the rest as there is alot of trich and no mycelium

the spots are more whitish with a green undertone, but the green is pretty visible

Edited by explosivekush (06/05/11 11:08 AM)

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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14564185 - 06/05/11 11:10 AM (12 years, 8 months ago)

any spot in myco for me without a pressure cooker? anyone can confirm that the boiling works for sterilizing

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Offlinetr8orjohn
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14564212 - 06/05/11 11:15 AM (12 years, 8 months ago)

UTSE for the PF-Tek, that'll show you how to reuse your jars.

I believe there are some other posts on here talking about how toxic metabolites from the trich can infiltrate into the resulting fruits, resulting in toxic shrooms. Whether this is correct or not, BRF and verm are cheap enough that it'll probably be your best bet to toss them and start over; if your looking for more of a kit-type approach, some of the sponsors sell spawn bags that are presterilized; a quick search will show you how to use those properly as well.

-jon

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Offlinewasting_space
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14564234 - 06/05/11 11:20 AM (12 years, 8 months ago)

If the contamination is in the area where u released innoculant than the innoculant is sour, or u did not flame sterilize the needle tip..  Even though all syringes sold claim to sterilized, its a good idea to sterilize the needle before use anyway..  If the contamination is all over in strange places than the jar itself was sour...  Best not to buy kits, DIY, at least that way if u fuck up, u will not feel as bad...


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OfflineMatty_Rulez
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14564259 - 06/05/11 11:28 AM (12 years, 8 months ago)

your syringe was probably contammed...  I got a bad B+ syringe once, and everything I innoc'd with it went green at the innoc sites...
even tried it on some agar, and they all went green....
if you use a good shroomery sponsor, they will usually replace the syringe for you...

good luck, man...

....  and yes..  if you're using PF-Tek...  you can get away with steam sterilizing them... 
PC's can be found pretty cheap, though...


--------------------
Life would be tragic if it weren't funny. - Stephen Hawking

Edited by Matty_Rulez (06/05/11 11:34 AM)

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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Matty_Rulez]
    #14564291 - 06/05/11 11:33 AM (12 years, 8 months ago)

i flame sterilized until red hot. id say they r more all over the place then at inoc sites but there were some around inoc sites.

where i got the syringe wasn't a sponsor but a shroomery member recommended me there i guess ill try to ask for another syringe.

but im not too sure if its the syringe because the day after i inoculated the cali mushroom kit thread popped up and it has the same guy that sold me my kit. Anyways another guy on there was saying that all his jars from that guy got contamed with trich too

ill try to get pics before i toss  , just gotta get this card reader 2 work

thx for all replys..

Edited by explosivekush (06/05/11 11:37 AM)

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Invisibleyutaka

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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14564312 - 06/05/11 11:39 AM (12 years, 8 months ago)

Quote:

explosivekush said:
...and i think i did a sterile procedure [ used face masks, latex gloves rubbed w/ alcohol, flame sterilized and wiped needle with alcohol, and also wiped the top of the lids with alcohol after taking foil off]...




Looks like another vector of contamination may have been wiping the needle after you flame sterilized it.

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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: yutaka]
    #14564372 - 06/05/11 11:55 AM (12 years, 8 months ago)

soz i should of changed the order of that, but i flame sterilized red hot after wiping the needle

it must be the jars, i doubt he sterilized them even though he said

"Jars are Pressure Sterilized at 15psi for 120 Minutes in our new Medical grade Autoclave, eliminating ANY possibility of contamination!"

lol bs.. anyways they took 5 days to come here and he says he shipped the same day that he sterilizes.

Edited by explosivekush (06/05/11 12:06 PM)

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OfflineNature Boy
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: tr8orjohn]
    #14565108 - 06/05/11 03:23 PM (12 years, 8 months ago)

Re-use jars with trich....WHY????  Jars are so friggin cheap.  Why risk the next round of time money and energy???

I say chuck those jars ASAP and start clean and fresh!  :2cents:

N.B.


--------------------
All submitted posts under this user name are works of pure fiction or outright lies.  Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit.  Note well:  Sorry, but I do not answer PM's unless you are a long-time trusted friend.  If you have a question, ask it in the appropriate thread.

                                                                               

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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Nature Boy]
    #14565695 - 06/05/11 05:53 PM (12 years, 8 months ago)

Well i got a just got my 60$ refunded atleast..

idk if it is really trich, my card reader isnt working im probably gunna try to get pics up before i toss

i would order from sponsors but none of them accept paypal or bank transfers. if im wrong on this pls tell me a sponsor with pre-sterilized jars that does accept paypal/banktransfers


Edited by explosivekush (06/05/11 05:55 PM)

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Offlineafrosheen
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14565748 - 06/05/11 06:06 PM (12 years, 8 months ago)

What's holding you back from making your own jars? BRF jars are simple, and grain jars are even simpler. You can get all the ingredients at a local grocery store.

BTW, I never saw one mention of a glovebox, which is what you should have been using to inoculate the jars. That's a big source of contamination right there, open air inoculation x 4 holes in lids.


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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? *DELETED* [Re: afrosheen]
    #14570342 - 06/06/11 04:56 PM (12 years, 8 months ago)

Post deleted by explosivekush

Reason for deletion: old


Edited by explosivekush (06/06/11 06:09 PM)

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Offlineoccollegeboi
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14571517 - 06/06/11 09:02 PM (12 years, 8 months ago)

Definitely trich!

What someone else said: It's best to just simply buy jars, brf, verm, and do it yourself. A LOT of people on here have stated that the kits are a waste of money and are very prone to contamination.

Also, use a glove box.

You may want to start out by watching the 4 video series on youtube for "Let's Grow Mushrooms PF Tek" and make sure you watch all 4.

If you have questions that arise, use the search function on the top right of this website, and if you can't find an answer, ask in the cultivation forum.

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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: occollegeboi]
    #14595895 - 06/11/11 12:41 PM (12 years, 8 months ago)

i've watched those vids, i've read so much teks and studied this subject on this site for about a month or two before i stared.

Well i made my own jars, sterilized in a vegetable steamer for 80 minutes. I inoculated 4 days ago, and all the jars are showing the same first signs as the old jars :frown: .

What happens is it starts of small white fuzzy spots , barely noticable unless you look up close, but they have a green undertone and yellow splotches around them. This is happening on all of my home-made jars.
:frown: it could be the syringe, i've talked to my vendor and they said they would replace for me.

:feelsbadman:

oh and those 2 other jars that i kept from the old batch , are now 80% green, and one has mycelium a bit bigger then the size of a quarter.

could colonization is a non-sterile spot cause contamination, even with micropore tape and a dry vermiculite layer? because the spot both batches colonized in are probably the most un-sterile spots in a house lol

also my temps from both batches were 70-85

Edited by explosivekush (06/11/11 12:48 PM)

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InvisibleDeepSpace9
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14595953 - 06/11/11 12:52 PM (12 years, 8 months ago)

Glove box isnt overkill. Most likely you shooting the jars up in an open air room is the problem.

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OfflineNature Boy
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14596080 - 06/11/11 01:29 PM (12 years, 8 months ago)

OMFG!!!  I TOLD you to get rid of those jars, PRONTO!!!  Your entire workspace is likely contaminated at this point.  YES, it escapes!!!  I don't care how tight the lid is on!!!

I'd say you are completely fucked at this point.  Well, you can always collect stamps. :facepalm:

N.B.


--------------------
All submitted posts under this user name are works of pure fiction or outright lies.  Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit.  Note well:  Sorry, but I do not answer PM's unless you are a long-time trusted friend.  If you have a question, ask it in the appropriate thread.

                                                                               

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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: Nature Boy]
    #14596100 - 06/11/11 01:33 PM (12 years, 8 months ago)

damn

:feelsbadman:

so should i just toss all the jars and start in a month or so

its a wierd contam on the other jars, it-s greenish grey with small solid yellow things on  - around it

Edited by explosivekush (06/11/11 01:53 PM)

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Offlineexplosivekush
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Re: 6 days after incoculation, all jars contamed w/ trich? [Re: explosivekush]
    #14596290 - 06/11/11 02:13 PM (12 years, 8 months ago)

i got my cam working , im gunna get some pics up atleast in about 10 mmins

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