Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Cultivation Supplies   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleFungal growth
Lootinint
Male User Gallery


Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
whatman syringe filters, please explain!
    #14561097 - 06/04/11 05:48 PM (12 years, 8 months ago)

they look pretty cool, but how does that tiny needle hole provide enough ge?
i realize lc's don't need much, but if 1/4 inch is the norm,
how does a wheel filter even come close?
and if it does, do lc's even need the ge at all, provided the
jar is 3/4 or less full?

Edited by Fungal growth (06/05/11 10:53 AM)

Extras: Filter Print Post Top
InvisibleTranscendingLife
I Don't Need a Life to Live
Male User Gallery


Registered: 06/09/10
Posts: 21,627
Trusted Cultivator
Re: wheel filters, please explain! [Re: Fungal growth]
    #14561713 - 06/04/11 08:11 PM (12 years, 8 months ago)

Are you talking about Whatmans?

LCs don't need GE.

You need GE port for LCs so you can pull the liquid into your syringe, cuz if it wasn't there, you'd have a vacuum. Thus, it would be impossible to extract the LC.

LCs do not require GE, because it's in liquid, but I feel it's beneficial for LCs to have a bit of GE.

See my Lid tek...I use Whatmans for agar dishes & LCs...


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: wheel filters, please explain! [Re: TranscendingLife]
    #14561756 - 06/04/11 08:19 PM (12 years, 8 months ago)

Quote:

todlow said:
LCs do not require GE, because it's in liquid, but I feel it's beneficial for LCs to have a bit of GE.




You still need the same amount of oxygen for the myc though.
There's some in the jar of course, and if you leave a lot of room that helps too.
Completely agree the biggest issue is equalizing pressure.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
InvisibleFungal growth
Lootinint
Male User Gallery


Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
Re: wheel filters, please explain! [Re: Doc_T]
    #14564109 - 06/05/11 10:50 AM (12 years, 8 months ago)

yes i mean whatmans, syringe filters.
sorry, the term 'wheel filter' is a leftover from my days in junkie hell.
ok, so they're main use is for equalizing pressure in lc's, not necessarily ge.
would'nt work on a grain jar then.

another mildly related question, i don't know myself but i've read that most contaminate spores are larger than cubensis spores.
with the proper micron size, could a syringe filter be used as
an extra precaution when innoculating with spores, filtering out the
trich and the cob while letting the cube by?

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: wheel filters, please explain! [Re: Fungal growth]
    #14564382 - 06/05/11 11:58 AM (12 years, 8 months ago)

Won't work on grain jars, correct. I like polyfil for grain jars.

Your second question is just wrong from the get-go, you don't shoot with a dirty syringe.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
InvisibleFungal growth
Lootinint
Male User Gallery


Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
Re: wheel filters, please explain! [Re: Doc_T]
    #14564526 - 06/05/11 12:33 PM (12 years, 8 months ago)

i have a few sfd's but when they're all in use i use poly.

right, but as no spore print is 100% sterile, no syringe can be either.
agar is tried and true, but there may be other ways not found yet.
but sadly, a little research turned out that cubensis spores are
considerably larger than trichoderma spores.

Extras: Filter Print Post Top
OfflineMycowlogist
Freethinker
Male


Registered: 06/29/08
Posts: 534
Loc: in a galaxy far far away....
Last seen: 10 years, 1 month
Re: wheel filters, please explain! [Re: Fungal growth]
    #14564694 - 06/05/11 01:28 PM (12 years, 8 months ago)

If you have an syringe port.  Why don't you sterilize a syringe stuffed with polyfill (without the plunger) and use it as a filter/equalizer.  Considering you have enough room in your syringe port for the 2 syringes.  When you don't need it any more remove the syringe filter.

If that makes sense?:cool:


--------------------
"And in the end, it's not the years in your life that count. It's the life in your years."  Honest Abe

Extras: Filter Print Post Top
InvisibleTranscendingLife
I Don't Need a Life to Live
Male User Gallery


Registered: 06/09/10
Posts: 21,627
Trusted Cultivator
Re: wheel filters, please explain! [Re: Mycowlogist]
    #14566801 - 06/05/11 10:02 PM (12 years, 8 months ago)

spores are larger than .2um.

Won't work to use it as a filter like that.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

Extras: Filter Print Post Top
InvisibleFungal growth
Lootinint
Male User Gallery


Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
Re: wheel filters, please explain! [Re: TranscendingLife]
    #14568935 - 06/06/11 11:19 AM (12 years, 8 months ago)


Quote:

todlow said:
spores are larger than .2um.

Won't work to use it as a filter like that.



no, it won't. the #'s i found show cube spores are considerably larger than trich spores. other spores run the gamut from .1um to 100um.

do you think the whatman's are superior than sfd's or poly?
kind of a moot point i guess since my contam rate hovers right around zero.

Edited by Fungal growth (06/06/11 11:20 AM)

Extras: Filter Print Post Top
InvisibleTranscendingLife
I Don't Need a Life to Live
Male User Gallery


Registered: 06/09/10
Posts: 21,627
Trusted Cultivator
Re: wheel filters, please explain! [Re: Fungal growth]
    #14569052 - 06/06/11 11:44 AM (12 years, 8 months ago)

Whatmans are the same um as SFDs, as long as you get the right ones.

Both should be .2 um.

Whatmans just have different applications than SFDs


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Cultivation Supplies   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Cheap permeant jar lid filter.
( 1 2 3 all )
SixTango 12,328 52 01/29/03 09:31 AM
by shroomologist
*
( 1 2 all )
matts 3,221 37 08/27/02 08:08 PM
by Roadkill
* Whatman syringe filters on grain/spawn jars?
( 1 2 all )
bombardier420 8,957 27 08/28/22 12:58 PM
by SarahP
* Coffee Filter Question redfoot 1,374 8 05/19/03 09:37 PM
by afoaf
* LC leaking through syringe filter? Guerrilla 610 7 05/10/21 04:19 AM
by Guerrilla
* Hepa type filter - flow hood - cheap & easy
( 1 2 all )
SixTango 16,419 25 04/09/02 11:14 AM
by rebelmoon
* Where's The Best Place To Get Whatman Filters? Please Update Links. Shroomer Smurf 1,698 11 03/30/10 10:54 AM
by JonEveryman88
* LC Lid w/ Whatman's Syringe Filter Question MFKDGAF 4,770 11 02/08/12 12:46 AM
by San

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,464 topic views. 46 members, 211 guests and 71 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.005 seconds on 12 queries.