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OfflineThe_Ghost
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Registered: 03/27/07
Posts: 15,802
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Question about failed liquid culture.
    #14560495 - 06/04/11 03:04 PM (12 years, 7 months ago)

So i followed this tek using a spore print:
http://www.shroomery.org/forums/showflat.php/Number/5238137#5238137

Scraped spores directly into the jar, bypassing syringe making.

But the spores never seemed to germinate. :crankey:

Its been like 3 weeks and alot of them are just sorta sitting at the bottom. Some chunkier clusters have formed but no real growth. The water still has honey in it so its not like there weren't enough nutrients. :shrug:

What gives?


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Question about failed liquid culture. [Re: The_Ghost]
    #14560578 - 06/04/11 03:20 PM (12 years, 7 months ago)

LC is lame, and even if the spores grow in it, you never know if it's non-contaminated until you inoculate grains or brf.  If you had inoculated brf cakes with those spores, you'd be at or near birthing by now.
RR


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OfflineBreakfast Crew
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Registered: 01/20/09
Posts: 1,985
Last seen: 12 years, 6 months
Re: Question about failed liquid culture. [Re: RogerRabbit]
    #14560596 - 06/04/11 03:24 PM (12 years, 7 months ago)

Try the the link in my signature. Preparing a grain jar is less work than pf and you will know if its contaminated.  Spores to LC will take x2 as much time, or longer, for a beginner.


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