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SirTripAlot
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Registered: 01/11/05
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First LC attempt
#14549836 - 06/02/11 07:52 AM (12 years, 8 months ago) |
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1. Was going to prepare via "Super Karo tek"
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/2321056/an/0/page/0
2. except the karo with be sucked into a syringe containing about 2cc of spores. (sterilized...heat up etc... Suck in a little air through a flame source after karo has been taken in)
3. let syringe incubate
4. incolate
any wrong steps?
-------------------- “I must not fear. Fear is the mind-killer. Fear is the little-death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.”
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scatmanrav
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Yes. The one where you put it in a syringe to grow.
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SirTripAlot
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Registered: 01/11/05
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Why would I not be able to inoculate a rye medium for instance, after colonization has taken place?
-------------------- “I must not fear. Fear is the mind-killer. Fear is the little-death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.”
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RogueTrippeR
Peaceful


Registered: 02/27/11
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Quote:
SirTripAlot said: Why would I not be able to inoculate a rye medium for instance, after ]colonization has taken place?
colonization won't take place?
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Cynosure
allow me to be your guide.


Registered: 10/06/09
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All of the teks I have read (and tried) seem to suggest putting all of your ingredients (nutrients, water, spores) into a sealed, sterile jar and let the spores do their thang. They tend to fill up syringes after the jar has some growth happening.
I don't see why your plan wouldn't work.. but I've failed at all of my LC attempts thus far.
-------------------- "You can peel it [language] off the ceiling and make it dance in front of you" - McKenna <3 .
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SirTripAlot
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Per the Tek I linked...if one is able to let spores germinate in a plactic container, why cant you let them germinate in a syringe chamber?
-------------------- “I must not fear. Fear is the mind-killer. Fear is the little-death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.”
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SirTripAlot
Semper Fidelis



Registered: 01/11/05
Posts: 7,598
Loc: Harmless (Mostly)
Last seen: 38 minutes, 51 seconds
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**germination
-------------------- “I must not fear. Fear is the mind-killer. Fear is the little-death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.”
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CheeseBurgler
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Registered: 03/31/11
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Quote:
SirTripAlot said: why cant you let them germinate in a syringe chamber?
that is a good point, but i think its a little back asswards. if you make a LC jar, then suck out some contents to germinate in the syringe, wont there still be contents in the jar doing that same thing as whats going on in the syringe?
what i mean to say is why add an extra step? the LC liquid is going to germinate, then be used to noc, so why store them in 2 separate containers (jar and syringe)?
not trying to be a dick, which is sometime hard with only a textual media, but if i were you, i'd let the jar germinate, THEN suck it up into a syringe.
great idea though (unique and out-of-the-box), and if you want to do it, then by all means, do it up. but this being your first go around with LC, i'd just leave it all in the jar till its ready (to be sucked out).
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scatmanrav
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Quote:
SirTripAlot said: Per the Tek I linked...if one is able to let spores germinate in a plactic container, why cant you let them germinate in a syringe chamber?
IME growth of LC is poor without good gas exchange. You'll get no gas exchange in a syringe. Less oxygen in the water to use up too (since theres less water). Thats why you grow your cultures somewhere else. It'll probably work out ok, but you wont be using a LC. And really at that time theres no point anyway. The point of a LC is to grow many syringes of material at once. You can just use the syringe right now, the spores will germinate on the rye or whatever, and you'll have growth in jars quicker.
Theres just no point to what you did, besides the fact that you'll get little to no growth.
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RogueTrippeR
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"...Less oxygen in the water to use up too (since there's less water)."
not only is there less water and no gas exchange, heated water holds less O to begin with. So, after you boil the O out of the water, contain it without gas exchange and then introduce more wasted air (burned of O since it's been drawn through a flame) you want to grow myc. It might work but I'm not sure why you would try.
This is way you posted. You knew something was off, just not sure what. So you posted to ask. 
Edited by RogueTrippeR (06/02/11 04:14 PM)
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