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 smith
Team B+
Registered: 03/21/03
Posts: 21
Loc: Michigan
Last seen: 14 years, 19 days
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 wbs question 
#1455149 - 04/13/03 07:34 PM (15 years, 15 days ago) |
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when you have quart jars prepared with wbs and ready to inoculate with a syringe, do you just stick the needle thru the tyvek filer and call it close enough?
or should one put another filter on the outside of the jar when your done?
thanks
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 SixTango
Mycota
Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
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Re: wbs question [Re: smith]
#1455224 - 04/13/03 08:02 PM (15 years, 15 days ago) |
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For any WBS jar that is going to get a syringe innoc, my foaf puts 2 holes in the lid. Innoc through one & tape it over.
With a 1 hole lid, loosen cap 1/4 turn. That moves the hole in the lid - on the tyvek - underneath.
Inject & tighten cap back up, which moves the hole in the lid AWAY from the hole in the tyvek. Then cover the lid with alc swabbed double coffee filter (allow alc to evap - before application) & hold them down with a rubber band.
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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~
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smith
Team B+
Registered: 03/21/03
Posts: 21
Loc: Michigan
Last seen: 14 years, 19 days
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Re: wbs question [Re: smith]
#1455246 - 04/13/03 08:12 PM (15 years, 15 days ago) |
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good idea thanks. one more qusetion, how may cc's do you think is about right to put in?
thanks again
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Oddish
Bulbasaur!
Registered: 04/12/03
Posts: 327
Last seen: 4 years, 7 months
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 Re: wbs question [Re: smith]
#1455303 - 04/13/03 08:46 PM (15 years, 15 days ago) |
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It is all up to you, man. You can do 1cc per jar or even up the dosage if you want them to colonize faster. Usually 1cc does the trick just fine.
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no-tone
Enema Bag Jones
Registered: 10/17/00
Posts: 1,091
Loc: Warm, Moist and Dark
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Re: wbs question [Re: SixTango]
#1455656 - 04/13/03 11:45 PM (15 years, 15 days ago) |
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Great post. It was very explanatory!
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Man thinks. God laughs. - Jewish Proverb
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SixTango
Mycota
Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
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Re: wbs question [Re: smith]
#1455754 - 04/14/03 12:17 AM (15 years, 15 days ago) |
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My foaf does around 5 or 6 quarts with a 12CC syringe.
BE CLEAN ABOUT IT ALL 
Shake the syringe, hard before innoc. Leave cap on while doing so (as if the syringe is clean & viable - waving an exposed needle around can pick up airborne contams. Swab needle with alc, or flame, between each jar. Once you punch in the syringe, swivel & squirt at eye level. So - you can see whatzzzzup.
5 little squirts. One in each corner of the jar - against the glass & one down the center. Then, do not shake. That way you can see if you get myc or any contam - when it germinates.
Shake around 15% colonization. Then, be patient. It takes a day or 3 to recover & takes off again. No need to shake again, IMHO.
6T 
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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~
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