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OfflineChowder
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Registered: 10/26/09
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Inoculating 27 half-pints with 2 syringes problem.
    #14545047 - 06/01/11 09:58 AM (12 years, 8 months ago)

Hi all

I am ready to sterlize and inoculate 27 half pint jars.
I have plenty of good spore prints but I only have 2 syringes.
I went in town today at all the pharmacies and none were selling syringes with needles.
So what can I do? Can I scape some spores into a shot-glass with water and use the syringes several times? Would that be prone to contaminants?

I could buy some from ebay, but I want to do it tonight.

Any suggestions?

Thanks!


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Offlinepcnubie
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Registered: 04/23/11
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Last seen: 12 years, 8 months
Re: Inoculating 27 half-pints with 2 syringes problem. [Re: Chowder]
    #14545088 - 06/01/11 10:14 AM (12 years, 8 months ago)

go to your local feed store or co-op and ask for large animal syringes and needles 10cc-20cc, most feed stores and lg. animal supply shops have them.

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Offlinetreewood69
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Re: Inoculating 27 half-pints with 2 syringes problem. [Re: pcnubie]
    #14545170 - 06/01/11 10:46 AM (12 years, 8 months ago)

You have to read before jumping into this kinda stuff and making way to many jars for spores you have.  Either buy another syringe or make an LC.  Your asking for problems by trying to split up 1 syringe into 2 but it can be done.  I would say only do 20 jars (assuming you got 2 10cc syringes).  and the other 7 jars will be ok until you buy another syringe or untill one of your jars get fully colonized. http://www.shroomery.org/67/Poor-Mans-Mycelium-Syringe-Tek


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InvisibleTacoHerder
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Re: Inoculating 27 half-pints with 2 syringes problem. [Re: treewood69]
    #14545555 - 06/01/11 12:36 PM (12 years, 8 months ago)

Simply take some clean water. Fill up a 1/4 or 1/2 pint jar. Use either a lid that will allow you to inject the spore solution into and retrieve. Or just a solid lid. Sterilize the jar for 15 min and let cool.  Work in a gb and inject the whole syringe into the jar. Then shake around and suck up your first 10cc.  That should extent the life of your spores.


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Invisibleslapphappypill
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Re: Inoculating 27 half-pints with 2 syringes problem. [Re: TacoHerder]
    #14545574 - 06/01/11 12:42 PM (12 years, 8 months ago)

Quote:

TacoHerder said:
Simply take some clean water. Fill up a 1/4 or 1/2 pint jar. Use either a lid that will allow you to inject the spore solution into and retrieve. Or just a solid lid. Sterilize the jar for 15 min and let cool.  Work in a gb and inject the whole syringe into the jar. Then shake around and suck up your first 10cc.  That should extent the life of your spores.



I'm tired and couldn't understand all that...

But OP, just suck up some sterile water into your used syringe (Flame the needle first). I try to use a jar of water thats been PC'd for 20 mins.

Once the water is in the sued syringe, shake it up real good and keep using it like it's a brand new one.

There are billions of spores in a single syringe, this will let you get a lot more out of them!


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I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

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Offlineasdfasdf


Registered: 07/26/10
Posts: 424
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Re: Inoculating 27 half-pints with 2 syringes problem. [Re: slapphappypill]
    #14545671 - 06/01/11 01:11 PM (12 years, 8 months ago)

Quote:

slapphappypill said:
Quote:

TacoHerder said:
Simply take some clean water. Fill up a 1/4 or 1/2 pint jar. Use either a lid that will allow you to inject the spore solution into and retrieve. Or just a solid lid. Sterilize the jar for 15 min and let cool.  Work in a gb and inject the whole syringe into the jar. Then shake around and suck up your first 10cc.  That should extent the life of your spores.



I'm tired and couldn't understand all that...

But OP, just suck up some sterile water into your used syringe (Flame the needle first). I try to use a jar of water thats been PC'd for 20 mins.

Once the water is in the sued syringe, shake it up real good and keep using it like it's a brand new one.

There are billions of spores in a single syringe, this will let you get a lot more out of them!





both these ideas are your best bet.


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To answer some of your questions...If by "ms" you mean "magic shrooms" then yes that's what I'm trying to grow"

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OfflineChowder
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Registered: 10/26/09
Posts: 256
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Re: Inoculating 27 half-pints with 2 syringes problem. [Re: asdfasdf]
    #14549423 - 06/02/11 04:50 AM (12 years, 8 months ago)

Thanks to you all guys. I'll do that!


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