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tryptonite
mushroom ninja



Registered: 02/06/04
Posts: 931
Loc: aussie
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question re: wild prints --> agar
#14534654 - 05/30/11 07:14 AM (12 years, 9 months ago) |
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sorry but I could not really find an answer to this question utse.
so I've just hacked up a plate of really nice pan subb mycelium in order to subculture more plates with H202 (4ml/L). but I was wondering if I did the right thing as the mycelium didnt even look like it was contaminated.
and my whenever I subculture mycelium to another plate it never does very well for some reason. usually slows to snail pace growth 
so the question is do wild prints always need to be subcultured to fresh plates even if no contamination is visible?
also I should mention the original culture is around a week old so was it too late anyway for H202 treatment?
Edited by tryptonite (05/30/11 07:19 AM)
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Nobitte
Student


Registered: 02/20/08
Posts: 493
Loc: Biosphere
Last seen: 6 years, 4 months
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Re: question re: wild prints --> agar [Re: tryptonite]
#14534669 - 05/30/11 07:22 AM (12 years, 9 months ago) |
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Do you use H2O2 in all your plates? I've heard its toxic to mycelium and its never really worked for me, but wait for a TC on that one.
I would instead just use multiple series of normal MDA plates or something similar to separate the desirable mycelium from contaminates.
-------------------- First we must learn... Then... WE CAN TEACH
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tryptonite
mushroom ninja



Registered: 02/06/04
Posts: 931
Loc: aussie
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Re: question re: wild prints --> agar [Re: Nobitte]
#14534685 - 05/30/11 07:28 AM (12 years, 9 months ago) |
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no peroxide in initial culture only in subcultures as its meant to kill all spores.
no contamination is visible mycelium looked good, so did I still need to subculture?
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Nobitte
Student


Registered: 02/20/08
Posts: 493
Loc: Biosphere
Last seen: 6 years, 4 months
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Re: question re: wild prints --> agar [Re: tryptonite]
#14534699 - 05/30/11 07:40 AM (12 years, 9 months ago) |
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I would spare them the peroxide, im sure it does kill spores, but it also hurts the poor mycelium.
If you have no visible contamination, begin to isolate and make series of aggressive/desirable sectors of growth from the mother-plate, dont use any more agar than you need to though, its a pretty big contamination vector if youre not totally setup with your sterile procedures.
-------------------- First we must learn... Then... WE CAN TEACH
Edited by Nobitte (05/30/11 07:42 AM)
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