Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Substrate Bags   Myyco.com Golden Teacher Liquid Culture For Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisibletryptonite
mushroom ninja
Male User Gallery


Registered: 02/06/04
Posts: 931
Loc: aussie
question re: wild prints --> agar
    #14534654 - 05/30/11 07:14 AM (12 years, 9 months ago)

sorry but I could not really find an answer to this question utse.

so I've just hacked up a plate of really nice pan subb mycelium in order to subculture more plates with H202 (4ml/L). but I was wondering if I did the right thing as the mycelium didnt even look like it was contaminated.

and my whenever I subculture mycelium to another plate it never does very well for some reason. usually slows to snail pace growth :shrug:

so the question is do wild prints always need to be subcultured to fresh plates even if no contamination is visible?

also I should mention the original culture is around a week old so was it too late anyway for H202 treatment?

Edited by tryptonite (05/30/11 07:19 AM)

Extras: Filter Print Post Top
OfflineNobitte
Student


Registered: 02/20/08
Posts: 493
Loc: Biosphere
Last seen: 6 years, 4 months
Re: question re: wild prints --> agar [Re: tryptonite]
    #14534669 - 05/30/11 07:22 AM (12 years, 9 months ago)

Do you use H2O2 in all your plates? I've heard its toxic to mycelium and its never really worked for me, but wait for a TC on that one.

I would instead just use multiple series of normal MDA plates or something similar to separate the desirable mycelium from contaminates.

:2cents:


--------------------
First we must learn...

Then... WE CAN TEACH :weirdeyes:

Extras: Filter Print Post Top
Invisibletryptonite
mushroom ninja
Male User Gallery


Registered: 02/06/04
Posts: 931
Loc: aussie
Re: question re: wild prints --> agar [Re: Nobitte]
    #14534685 - 05/30/11 07:28 AM (12 years, 9 months ago)

no peroxide in initial culture only in subcultures as its meant to kill all spores.

no contamination is visible mycelium looked good, so did I still need to subculture?


--------------------

Entheogen Trade List

Extras: Filter Print Post Top
OfflineNobitte
Student


Registered: 02/20/08
Posts: 493
Loc: Biosphere
Last seen: 6 years, 4 months
Re: question re: wild prints --> agar [Re: tryptonite]
    #14534699 - 05/30/11 07:40 AM (12 years, 9 months ago)

I would spare them the peroxide, im sure it does kill spores, but it also hurts the poor mycelium.

If you have no visible contamination, begin to isolate and make series of aggressive/desirable sectors of growth from the mother-plate, dont use any more agar than you need to though, its a pretty big contamination vector if youre not totally setup with your sterile procedures.


--------------------
First we must learn...

Then... WE CAN TEACH :weirdeyes:

Edited by Nobitte (05/30/11 07:42 AM)

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Substrate Bags   Myyco.com Golden Teacher Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* Wild Prints for innoculation... mammoth_hunter 2,340 17 09/12/02 06:59 PM
by mammoth_hunter
* wild print vs. domesticated AbeZard 1,460 3 08/25/01 07:43 AM
by Zen Peddler
* Question about printing on foil Ripple 1,199 8 08/24/02 12:49 PM
by mickey_rourke
* Question for PRINT buyers Cow Shit Collector 793 5 05/08/01 12:42 AM
by ChangLee
* Question about prints chronic_124 476 2 04/01/02 08:48 AM
by luvdemshrooms
* question about prints BongHit 514 2 05/04/01 04:42 PM
by hatter
* Quick questions RE: Practicalities of Agar pouring Heruuka 1,600 5 10/10/02 02:22 AM
by Anno
* Question about agar supplies Chemical_Smile 1,546 3 04/20/02 05:50 AM
by babyshroom

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
431 topic views. 22 members, 238 guests and 76 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.01 seconds on 14 queries.