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buddy111
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Registered: 05/29/11
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Last seen: 3 years, 7 months
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how long can a spore print survive?
#14533182 - 05/29/11 09:38 PM (12 years, 9 months ago) |
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I just found a whole bunch of old spore prints that i had in an envelope for the last 10years
what are the odds that they might still produce life?
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 6 months
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Re: how long can a spore print survive? [Re: buddy111]
#14533239 - 05/29/11 09:48 PM (12 years, 9 months ago) |
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I've fruited Ps. cyan from 15 year old prints that I'd kept in the fridge the whole time. I soaked some of the spores in sterile water for a month or so before plating them on agar. Mostly you need to rehydrate them, but I've also heard that cubensis spores don't keep as well...it's easier to just get fresh prints. 
PS
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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mycoscopy
Mushkateer


Registered: 05/29/11
Posts: 456
Loc: TX
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Re: how long can a spore print survive? [Re: PrimalSoup]
#14533250 - 05/29/11 09:51 PM (12 years, 9 months ago) |
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I'd try them anyway just to see, soak them like PS said, it'd be fun to see what comes of it!
-------------------- Mushroom nerd, future farmer, lab rat. Anything I say about psilocybes is purely hypothetical.
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BrandNewbie
Captain



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Re: how long can a spore print survive? [Re: buddy111]
#14533587 - 05/29/11 11:12 PM (12 years, 9 months ago) |
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I think that you'll find that ten year old spores are more mature and a whole lot wiser...

(They ARE tougher than you'd think though. Definitely let 'em soak for a spell first.)
-------------------- Question: Why do women wear make-up and perfume? Answer: Because they're ugly and they stink.
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buddy111
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Registered: 05/29/11
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Re: how long can a spore print survive? [Re: BrandNewbie]
#15374607 - 11/15/11 02:12 PM (12 years, 3 months ago) |
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I was delayed in my project and so considering trying them again.
The way I used to do it was to just sprinkle the spores right onto the substrate. no syringe.
can't find a description of that tek now. anyone know where it is?
so if i do this soaking thing what am I doing differently?
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 6 months
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Re: how long can a spore print survive? [Re: buddy111]
#15376803 - 11/15/11 10:02 PM (12 years, 3 months ago) |
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You're preparing sterilized water in some container, and introducing spores from the prints to it under sterile conditions, and then waiting a month or so, after which you take tiny amounts of the visible spores and try to germinate them on agar plates. At least that's the way I've done it.
Perhaps you could just saturate the spore print, wait a while, let it dry out, and then try your normal technique. Dunno. 
PS
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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buddy111
Stranger
Registered: 05/29/11
Posts: 3
Last seen: 3 years, 7 months
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Re: how long can a spore print survive? [Re: PrimalSoup]
#15379434 - 11/16/11 01:47 PM (12 years, 3 months ago) |
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right.. well it's minimal risk so i guess i might just try to do it the way I've always done it and hope that there is enough moisture in the substrate to adequately hydrate those little spores and come to life. they are magic afterall.
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