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OfflineAdam553
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Registered: 11/23/09
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new to agar
    #14532657 - 05/29/11 07:53 PM (12 years, 8 months ago)

can contaminants be white like myc? there is some tell tail signs of rhyzomophric growth (spelling?) aside from my inoculated dowels in some of my petri dishes, and its white, i'm wondering if it is from myc falling off of my wooden dowel that i placed in the dish or if it is contamination.

my procedure was sterile, but unfortunately i don't have a HEPA filter or anything like that.... should i expect contamination?


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InvisibleDoctor_Inoc
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Registered: 04/30/11
Posts: 646
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Re: new to agar [Re: Adam553]
    #14532738 - 05/29/11 08:09 PM (12 years, 8 months ago)

Trichoderma mycelium begins white before it sporulates, turning green.

Sounds to me like you either have satellite colonies (contamination) or pieces of myce have fallen off of the dowel, inoculating the agar.
(As you suggested).
Pics would help.


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OfflineAdam553
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Re: new to agar [Re: Doctor_Inoc]
    #14532778 - 05/29/11 08:16 PM (12 years, 8 months ago)

i'll try to get pics tommorow. as it is though i only have a cellphone for pictures, is that going to be good enough to determine if it is contam or not?


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InvisibleDoctor_Inoc
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Re: new to agar [Re: Adam553]
    #14532820 - 05/29/11 08:27 PM (12 years, 8 months ago)

Worth a gamble.

Won't really know until you try it sir.
If my eyes can't determine (due to pic. quality), there are plenty of other experienced hands with better eyes then me.
(They all probably have bigger computer monitors then me too :smile2: ).
Those individuals may be able to see the pictures clearer then me.


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OfflineAdam553
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Re: new to agar [Re: Doctor_Inoc]
    #14532855 - 05/29/11 08:33 PM (12 years, 8 months ago)

i'll give it a shot tomorrow then.


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OfflineTerry M
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Re: new to agar [Re: Adam553]
    #14532893 - 05/29/11 08:37 PM (12 years, 8 months ago)

Quote:

Adam553 said:
my procedure was sterile, but unfortunately i don't have a HEPA filter or anything like that.... should i expect contamination?



I hope you're using a glove box. Otherwise don't bother to try and grow anything on agar -- contaminants will beat you.

Are you trying to inoculate agar from colonized dowels? I tried this and finally gave up because the dowel culture I got was so contaminated. But I guess it's good enough for logs.


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OfflineAdam553
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Re: new to agar [Re: Terry M]
    #14532925 - 05/29/11 08:44 PM (12 years, 8 months ago)

i have a still air box with tyvek sleeves and gloves that i wear, but nothing is sealed. i dont have one of those cool glove boxes that i see in here that are all self contained.


sterilized the area, inside the box, and flame sterilized the needle i used when i tried a MS syringe with the agar plate. i use clorox whipes because i think the bleach kills more than alcohol. although i use 70% iso. alcohol too.


if you had bad experience with the dowels then i'm guessing its contam from them or the air... or me screwing up somewhere.


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