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chronicdruid
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Registered: 04/29/09
Posts: 112
Last seen: 4 years, 7 months
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Re: UK supplies thread. [Re: deucedbi9]
#14744836 - 07/10/11 10:58 AM (12 years, 6 months ago) |
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hi dueced, cant enlarge ur pic atm, but myne looks different. its blue ontop, white underneath. and dont see the need to seperate it into different layers.
take a look at the pics, made some test jars, but dont have a culture to inoculate them with yet. the lids just have 2 1/2 inch holes 1 has an injection port the other has a piece of the tyvek siliconed over it. so there is no problem undoing lids like you suggested? im pretty sure it is breathable or allows acertin amount of gas exchange and is also hydrophobic, which is perfect for mycology.
well it stayed fine after 1 pressure cook for 90 + mins at 15psi
think it should be a fine material to use, but will test several jars first before making them all like this.
Edited by chronicdruid (07/10/11 11:03 AM)
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chronicdruid
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Registered: 04/29/09
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Last seen: 4 years, 7 months
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does any1 know anywhere to get parafilm at a reasonable price... (cheap as possible) if any1 uses an alternative could they please post what brand or type they use. tempted to try clingfilm as parafilm is so expensive, but i dont want to ruin experiments just because i skimped on the parafilm.
also is the green parafilm for florists any good for sealing dishes? says, its waterproof and stretchable, but guess it might not be breathable. dam cheap though.
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FractalXplora
Grainiack




Registered: 02/11/06
Posts: 2,494
Loc: UK
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theres no subsitute for proper parafilm, last time I needed some myself and a couple of uk dudes on here went 3rds on a roll.
Worked out about 11 quid each.
Got it last summer my 3rd is still going, not used it all yet, but getting there 
from here
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chronicdruid
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Registered: 04/29/09
Posts: 112
Last seen: 4 years, 7 months
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cheers bro, will have to invest. some of these lab supply website crack me up... its £105 + vat. for the parafilm dispenser!! how did they work that out XD
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ozzysmygod
Late Night PC'ing Enthusiast



Registered: 11/26/08
Posts: 835
Last seen: 9 years, 3 months
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Micropore tape worked for me on some test plates. But parafilm is the one!
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badman

Registered: 06/14/06
Posts: 4,039
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Quote:
chronicdruid said: cheers bro, will have to invest. some of these lab supply website crack me up... its £105 + vat. for the parafilm dispenser!! how did they work that out XD
I know its robbery, they normally overcharge for anything lab related. TBH the dispenser is only needed if you do a fuck load of plates, a clean pair of scissors is all you really need. Id suggest using the 100 mm wide roll.
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ozzysmygod
Late Night PC'ing Enthusiast



Registered: 11/26/08
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Re: UK supplies thread. [Re: badman]
#14752302 - 07/11/11 04:27 PM (12 years, 6 months ago) |
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100mm really? I use that at the moment and was considering getting 50mm next time as i currently cut it in half before use.
You could build your own dispenser if your set on it. Spare blades for the ones they sell cost £5 so you could use it to build one
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badman

Registered: 06/14/06
Posts: 4,039
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Re: UK supplies thread. [Re: ozzysmygod]
#14753099 - 07/11/11 07:13 PM (12 years, 6 months ago) |
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100 mm all the way mate. I use the red for standard dishes, one piece will go around twice. I use one of the reds for a 'work' plate and two for a 'good'. A red piece will also do slants nicely. Green for small dishes and half a red for booze proofing labels check left v right slant.
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chronicdruid
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Registered: 04/29/09
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Re: UK supplies thread. [Re: badman]
#14756345 - 07/12/11 11:16 AM (12 years, 6 months ago) |
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no i didnt want the dispenser, im quite happy with scissors or just tearing with my hands. i was just noting how ridiculous the price of it was.
nice slants there badman
think i might try the 100mm cutting it width wise appeals to me. peace
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deucedbi9
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Registered: 10/24/06
Posts: 4,594
Loc: UK
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Quote:
chronicdruid said: ...if any1 uses an alternative tempted to try clingfilm as parafilm is so expensive, but i dont want to ruin experiments just because i skimped on the parafilm.
Ive not used parafilm for the same reason.cost. Started out using cellotape (i shit you not) the last inch of the dish was micropore tape(again relative expence)
Now use only cling film. Cut ~ 2" off the end of roll. Extreemly easy to use. It may dry out, after a time, but is usefull and cheap for short term storage and propagation of a strain.
-------------------- whether low pressure sucks or high pressure blows... it's a bugger to cycle in. even though I'm feeling good Something tells me I'd better activate my prayer capsule
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ozzysmygod
Late Night PC'ing Enthusiast



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Re: UK supplies thread. [Re: deucedbi9]
#14761035 - 07/13/11 06:06 AM (12 years, 6 months ago) |
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Parafilm is about £15 a roll is it not?
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deucedbi9
Stranger

Registered: 10/24/06
Posts: 4,594
Loc: UK
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Re: UK supplies thread. [Re: ozzysmygod]
#14761154 - 07/13/11 07:04 AM (12 years, 6 months ago) |
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Quote:
ozzysmygod said: Parafilm is about £15 a roll is it not?
Not sure.When i looked for some online i just baulked at the price and found an adequate alternative.
-------------------- whether low pressure sucks or high pressure blows... it's a bugger to cycle in. even though I'm feeling good Something tells me I'd better activate my prayer capsule
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ozzysmygod
Late Night PC'ing Enthusiast



Registered: 11/26/08
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Last seen: 9 years, 3 months
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Re: UK supplies thread. [Re: deucedbi9]
#14761408 - 07/13/11 09:20 AM (12 years, 6 months ago) |
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Have you used it before? Its actually amazing, well worth £15 imo and it will last for ages
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deucedbi9
Stranger

Registered: 10/24/06
Posts: 4,594
Loc: UK
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Re: UK supplies thread. [Re: ozzysmygod]
#14762335 - 07/13/11 12:40 PM (12 years, 6 months ago) |
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Quote:
ozzysmygod said: Have you used it before? Its actually amazing, well worth £15 imo and it will last for ages
As long as a £1 a time roll of 12inch clingfilm, cut into 2 inch sections? As stated. I have never used parafilm.Perhaps, were more professionaly minded, i might try it,but at the moment i would prefer to use my income on petri's, and other essentials, rather than the parafilm to wrap them in.
-------------------- whether low pressure sucks or high pressure blows... it's a bugger to cycle in. even though I'm feeling good Something tells me I'd better activate my prayer capsule
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ozzysmygod
Late Night PC'ing Enthusiast



Registered: 11/26/08
Posts: 835
Last seen: 9 years, 3 months
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Re: UK supplies thread. [Re: deucedbi9]
#14762628 - 07/13/11 01:37 PM (12 years, 6 months ago) |
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Praps not as cheap as clingfilm no! :p
If it works for you mate, then go with it. I wasnt knocking you!
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LousyHero
California Dreamin'



Registered: 06/05/11
Posts: 72
Loc: Laandon!, UK
Last seen: 10 years, 10 months
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Re: UK supplies thread. [Re: ozzysmygod]
#14763175 - 07/13/11 03:13 PM (12 years, 6 months ago) |
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guys what's that stuff you add to syringe water to stop spores from clumping up and sticking to the sides?
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  !!10ml / 50ml syringes / needles for trade UK!!
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ozzysmygod
Late Night PC'ing Enthusiast



Registered: 11/26/08
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Last seen: 9 years, 3 months
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Re: UK supplies thread. [Re: LousyHero]
#14763216 - 07/13/11 03:20 PM (12 years, 6 months ago) |
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a drop of sex lube or washing up liquid. Ive never used it though.
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LousyHero
California Dreamin'



Registered: 06/05/11
Posts: 72
Loc: Laandon!, UK
Last seen: 10 years, 10 months
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Re: UK supplies thread. [Re: ozzysmygod]
#14763626 - 07/13/11 04:57 PM (12 years, 6 months ago) |
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cool, thanks.. well I will.. sucked some spores into the syringe and then realized that big part got stuck to the shot glass walls..
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  !!10ml / 50ml syringes / needles for trade UK!!
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ozzysmygod
Late Night PC'ing Enthusiast



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Re: UK supplies thread. [Re: LousyHero]
#14765981 - 07/14/11 01:09 AM (12 years, 6 months ago) |
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You'll still have spores in your syringe dont worry
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Gigabyt3r
Stranger

Registered: 05/24/11
Posts: 58
Last seen: 12 years, 6 months
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Re: UK supplies thread. [Re: ozzysmygod]
#14766894 - 07/14/11 09:20 AM (12 years, 6 months ago) |
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-------------------- "Ummm, you have to be a run-of-the-mill dipshit to use popcorn as your substrate, and sterilize it in the microwave" <3 Shiitake's
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