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Offlineglitche
Genetic Engineer


Registered: 04/07/11
Posts: 241
Last seen: 8 years, 3 months
Are my Jars okay?
    #14528424 - 05/28/11 10:04 PM (12 years, 9 months ago)

I followed a tek on here how to make jars by drilling a hole in the top and putting polyfil in the hole as an inoculation point. I understand it isn't the best and most sterile but I'm working with what I can afford for now. I wanted some advice on these jars I'm a total n00b so I'm desperate for some advice. Thank you in advance









Also, Do you think there is too much moisture inside of this, I'm using WBS as a substrate.


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Offlinechal420
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Re: Are my Jars okay? [Re: glitche]
    #14528450 - 05/28/11 10:12 PM (12 years, 9 months ago)

what do you think is wrong with them? the pics are kinda bad hard to tell

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Offlineglitche
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Registered: 04/07/11
Posts: 241
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Re: Are my Jars okay? [Re: chal420]
    #14528468 - 05/28/11 10:17 PM (12 years, 9 months ago)

I'm not sure I think I did everything correctly but I just wanted a second opinion. I did the WBS tek and it's been 4 days and I don't see any growth, I know its still very early in the game I guess I'm just paranoid being that I'm a n00b.  I'll upload some better pics.

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Invisiblethor_ak
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Registered: 04/03/02
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Re: Are my Jars okay? [Re: glitche]
    #14528494 - 05/28/11 10:23 PM (12 years, 9 months ago)

Looks like its a little wet. What tek did you use?


--------------------
"A smart man learns from his mistakes. A wise man, learns from the mistakes of others. A woman, makes you pay for these mistakes."

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Offlineglitche
Genetic Engineer


Registered: 04/07/11
Posts: 241
Last seen: 8 years, 3 months
Re: Are my Jars okay? [Re: glitche]
    #14528517 - 05/28/11 10:28 PM (12 years, 9 months ago)

I used Doc's the only thing I did differently was I didn't take out the birdseed that floated. There were only 4 Jars that look  like that out of the 24 the rest of them don't look as moist.

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Offlineglitche
Genetic Engineer


Registered: 04/07/11
Posts: 241
Last seen: 8 years, 3 months
Re: Are my Jars okay? [Re: glitche]
    #14528542 - 05/28/11 10:33 PM (12 years, 9 months ago)

I just let the Polyfil hang out on the outside but on the inside it's tight and looks like a lil nub.

Hopefully these pics are better?





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Invisiblethor_ak
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Re: Are my Jars okay? [Re: glitche]
    #14528561 - 05/28/11 10:36 PM (12 years, 9 months ago)

I haven't done to much WBS, but I think that might be too moist. And if

it is, there probably isn't anything you can do to help it. I'd wait for

someone elses opinion, cause like I said, Im not an expert. So you just

soaked, and simmered, then PC'ed? That seems like an awful lot of water

in there.


--------------------
"A smart man learns from his mistakes. A wise man, learns from the mistakes of others. A woman, makes you pay for these mistakes."

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Offlineglitche
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Registered: 04/07/11
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Last seen: 8 years, 3 months
Re: Are my Jars okay? [Re: glitche]
    #14528603 - 05/28/11 10:43 PM (12 years, 9 months ago)

oooooooh, I just remembered why only 4 were like that. I tried 2 different teks one of the teks said to boil the WBS before pressure cooking and I guess I didn't let them dry out enough. Thanks man Sorry, I need to start labeling my jars  :sorry:

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OfflineAlex22
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Registered: 04/17/11
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Re: Are my Jars okay? [Re: glitche]
    #14529016 - 05/29/11 12:48 AM (12 years, 9 months ago)

4 days isn't unusual to not see any growth. It could just be the spores, the temperature being lower than perfect, or a host of other things.

For example I bought a syringe from a non shroomery vendor and it has been 10 days and I'm just seeing the first growth in little splotches.

In comparison I took a spore print (my first ever woohoo) and inoculated four jars of the exact same batch of substrate and three of them are about 65% colonized in the same amount of time with the other showing good growth as well.

Wait a little longer before you panic.


--------------------
http://gaianmushroommind.webs.com/articles.htm

Trying to start a movement. Here is the first article. Excerpt:


" Imagine if I told you that you could use the alphabet but only from G to V. That sounds ridiculous right? Then why is it okay to live our lives within the parameters others set? There is an entire world of experience that we are being denied."

Read it, let me know what you think, PM me.

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Offlineglitche
Genetic Engineer


Registered: 04/07/11
Posts: 241
Last seen: 8 years, 3 months
Re: Are my Jars okay? [Re: Alex22]
    #14529036 - 05/29/11 12:55 AM (12 years, 9 months ago)

Quote:

Alex22 said:
4 days isn't unusual to not see any growth. It could just be the spores, the temperature being lower than perfect, or a host of other things.

For example I bought a syringe from a non shroomery vendor and it has been 10 days and I'm just seeing the first growth in little splotches.

In comparison I took a spore print (my first ever woohoo) and inoculated four jars of the exact same batch of substrate and three of them are about 65% colonized in the same amount of time with the other showing good growth as well.

Wait a little longer before you panic.




Congrats man, I can't wait till i get my first spore print from my own shrooms =). I also can't wait until I see some signs of mycelium. I know I'm being impatient I'm more nervous than anything being that this is my first time.

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Offlinerunningfox2002
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Re: Are my Jars okay? [Re: glitche]
    #14529038 - 05/29/11 12:56 AM (12 years, 9 months ago)

It seems like it is alot of moisture.  My spores took a lot longer but they allfinally colonized.  Be patient...all good things come from it :smile:.  Also...if you have that many jars, id seriously try to do better than polyfil.  Alot of chance for contams that way.  Its good for fae from what ive read, but not for ge.  Id hate to see it all contam lol.


--------------------
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Anything I say or talk about is strictly for my own search of knowledge and to satisfy my thirst of curiosity.

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Invisiblethor_ak
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Re: Are my Jars okay? [Re: Alex22]
    #14529040 - 05/29/11 12:56 AM (12 years, 9 months ago)

Yea, I've gotten bum spores too from non vendors. The only thing I can

suggest is to just wait. I'd say wait 10 days and if you don't see

anything, or you notice a bad smell, start over. By the looks of it you

shook the jars after you PC'd them, and after you inoculated? To hot/cold

temps will hinder your growth massively. Did you have the tops covered

while you PC'd them? How much water did you use in your PC?


--------------------
"A smart man learns from his mistakes. A wise man, learns from the mistakes of others. A woman, makes you pay for these mistakes."

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OfflineAlex22
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Re: Are my Jars okay? [Re: glitche]
    #14529049 - 05/29/11 12:59 AM (12 years, 9 months ago)

Its normal. My first grow I was checking my jars every day, worrying and wondering. Six of them actually never showed any growth at all but six made it and I got a pretty good haul. From now on I'm only ordering from sponsors here because of the success others have had.

I'm doing my second now and trust me the nerves go way down as time goes on. They do look a little wet inside but mine looked like that first grow too and it ended up okay. I'm sure you will be too, and if not then at least you can learn something and try again :grin:


--------------------
http://gaianmushroommind.webs.com/articles.htm

Trying to start a movement. Here is the first article. Excerpt:


" Imagine if I told you that you could use the alphabet but only from G to V. That sounds ridiculous right? Then why is it okay to live our lives within the parameters others set? There is an entire world of experience that we are being denied."

Read it, let me know what you think, PM me.

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Offlineglitche
Genetic Engineer


Registered: 04/07/11
Posts: 241
Last seen: 8 years, 3 months
Re: Are my Jars okay? [Re: Alex22]
    #14529088 - 05/29/11 01:23 AM (12 years, 9 months ago)

Quote:

runningfox2002 said:
It seems like it is alot of moisture.  My spores took a lot longer but they allfinally colonized.  Be patient...all good things come from it :smile:.  Also...if you have that many jars, id seriously try to do better than polyfil.  Alot of chance for contams that way.  Its good for fae from what ive read, but not for ge.  Id hate to see it all contam lol.




Yeah, that definitely wouldn't be good if they all got contaminated I'm gonna make better Jars out of them after I see how this works first. Thanks you.

Quote:

thor_ak said:
Yea, I've gotten bum spores too from non vendors. The only thing I can

suggest is to just wait. I'd say wait 10 days and if you don't see

anything, or you notice a bad smell, start over. By the looks of it you

shook the jars after you PC'd them, and after you inoculated? To hot/cold

temps will hinder your growth massively. Did you have the tops covered

while you PC'd them? How much water did you use in your PC?





I pressure cooked them for 90 minutes at a psi of 15 and I filled the PC up to the first line what it said to do in my PC handbook.

I let them cool for a day after they were pressure cooked and shook each of them after I took them out.

I didn't shake them after I inoculated them because I heard you are suppose to do that only after you see 20% growth of mycelium which I read from a tek on here.

The tops were covered with aluminum foil while I pressure cooked them so the water wouldn't get onto the polyfil I heard wet polyfil would make it very easy for germs to contam the jars.


Quote:

Alex22 said:
Its normal. My first grow I was checking my jars every day, worrying and wondering. Six of them actually never showed any growth at all but six made it and I got a pretty good haul. From now on I'm only ordering from sponsors here because of the success others have had.

I'm doing my second now and trust me the nerves go way down as time goes on. They do look a little wet inside but mine looked like that first grow too and it ended up okay. I'm sure you will be too, and if not then at least you can learn something and try again :grin:




haha, thanks man hopefully this goes well so I don't have to worry more but i guess it is all part of the learning experience thank you =) and congrats on your hauls hopefully I will get some good ones down the line.

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Invisiblejokefox
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Re: Are my Jars okay? [Re: glitche]
    #14529092 - 05/29/11 01:24 AM (12 years, 9 months ago)

Quote:

glitche said:
I just let the Polyfil hang out on the outside but on the inside it's tight and looks like a lil nub.

Hopefully these pics are better?








bacteria ewwwwwwwwwwwwwwwwwwwwwwwwwwwwww

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InvisibleLuger0815
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Re: Are my Jars okay? [Re: jokefox]
    #14529113 - 05/29/11 01:32 AM (12 years, 9 months ago)

i wonder how it smells inside....
take a sniff...and tell us if you would eat it.

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Invisiblejokefox
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Re: Are my Jars okay? [Re: Luger0815]
    #14529116 - 05/29/11 01:33 AM (12 years, 9 months ago)

its bacteria, i can tell , just look at the slime

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InvisibleLuger0815
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Re: Are my Jars okay? [Re: jokefox]
    #14529123 - 05/29/11 01:34 AM (12 years, 9 months ago)

yes, looks gross man...

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InvisibleShadOWCrack
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Re: Are my Jars okay? [Re: jokefox]
    #14529463 - 05/29/11 05:07 AM (12 years, 9 months ago)

glitche said:
I'm not sure I think I did everything correctly but I just wanted a second opinion. I did the WBS tek and it's been 4 days and I don't see any growth, I know its still very early in the game I guess I'm just paranoid being that I'm a n00b.  I'll upload some better pics.



What did you use to knock them up? Spores,:syringe: LC?
Spores can take 3-7 days to germinate. a :syringe: is a little faster because the spores are hydrated. i have seen growth with an LC in 12hrs.
Quote:

jokefox said:
Quote:

glitche said:
I just let the Polyfil hang out on the outside but on the inside it's tight and looks like a lil nub.

Hopefully these pics are better?








bacteria ewwwwwwwwwwwwwwwwwwwwwwwwwwwwww



:imwithstupid: You might want to move that and any jar that looks like that away from your other jars.

watch this jar too it could be to much h2o, but also bacteria will generate heat and cause it too. just keep an eye on it.

:goodluck:


--------------------
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:opensign: VVV Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV :opensign:

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InvisibleShadOWCrack
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Re: Are my Jars okay? [Re: ShadOWCrack]
    #14529465 - 05/29/11 05:09 AM (12 years, 9 months ago)

ps

if those jars are the ones you boiled it could be starch but i get bac. when that happens.

need any help just hit me up


--------------------
AMU=Amateur Mycologists United

AMU ------->:opensign:--->:goatse:<---:opensign:<------Q&A
:squirrelnut:I'm just a squirrel man, trying to get a nut:urinate:

:v::v:

:opensign: VVV Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV :opensign:

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