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Offlinezzantium2999
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spores to agar-will this work?
    #14527607 - 05/28/11 06:34 PM (12 years, 8 months ago)

Could I put cubensis spores on agar and get colonization? I know you can with azureus spores. Also, If I can, is it possible to use a syringe or will it work with just a print? If you don't have an answer to This question, DONT POST ANYTHING!!! I know this sounds silly, and everyone is waiting to say "Thats stupid. What you want to do is...." What I would like is this questioned answered without any stupid comments on why this sounds dumb to try. THANK YOU :grin:


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OfflineScarab74
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Re: spores to agar-will this work? [Re: zzantium2999]
    #14527622 - 05/28/11 06:40 PM (12 years, 8 months ago)

Yes, but you need to do a sterile tranfer from the print by flaming a wire loop (we like lighting alcohol wipes in a glass bowl) till it glows red.  Then scape it across the print followed by scraping it across the agar. Open the agar only just before the scrape then close immediately.

If you stay sterile then you should have a cube-only culture growing pretty soon!


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~Scarab74
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InvisibleDoc_T
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Re: spores to agar-will this work? [Re: zzantium2999]
    #14527630 - 05/28/11 06:41 PM (12 years, 8 months ago)

Quote:

zzantium2999 said:
"What you want to do is...."




What you want to do is watch Roger Rabbit's videos.
All of them... but start with the agar one.


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You make it all possible. Doesn't it feel good?


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Offlinezzantium2999
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Re: spores to agar-will this work? [Re: Doc_T]
    #14531866 - 05/29/11 05:25 PM (12 years, 8 months ago)

lol yea I want to buy the videos but don't have a credit card right now. Hoping to find an online copy or trade someone for them. To bad the agar one isn't online


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A good friend would bail you out of jail, but your best friend would be the one sitting next to you saying, "damn that was awesome".


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Offlinek00laid
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Re: spores to agar-will this work? [Re: zzantium2999]
    #14531879 - 05/29/11 05:28 PM (12 years, 8 months ago)

what you want to do is drop spores onto agar.

then they germinate.

then you transfer to new agar,

then you transfer a again. then let it grow a bit and look for sectors.


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AMU - AMU Q & A - MyVideo Teks!


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Offlinezzantium2999
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Re: spores to agar-will this work? [Re: zzantium2999]
    #14531883 - 05/29/11 05:28 PM (12 years, 8 months ago)

well it turns out that they are. Plus some more cultivation vids from R.R. HERE THEY ARE


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A good friend would bail you out of jail, but your best friend would be the one sitting next to you saying, "damn that was awesome".


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Offlinek00laid
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Re: spores to agar-will this work? [Re: zzantium2999]
    #14531888 - 05/29/11 05:29 PM (12 years, 8 months ago)

those same videos are on www.mushroomvideos.com

and theyr all just demos, even the ones on the youtube vid. they are the same demos.


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AMU - AMU Q & A - MyVideo Teks!


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OfflineRogerRabbitM
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Re: spores to agar-will this work? [Re: zzantium2999]
    #14531979 - 05/29/11 05:49 PM (12 years, 8 months ago)

Quote:

zzantium2999 said:
lol yea I want to buy the videos but don't have a credit card right now.




You can send a money order to the address on the bottom of each page of mushroom videos.  We'll get your full release on 2 DVDs right out when received.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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