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OfflineBuckeye Oysters
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Can you make agar slurry to inoculate outdoor hpoo?
    #14527324 - 05/28/11 05:41 PM (12 years, 8 months ago)

I had a few extra fully colonized dishes of agar that I put 3 dishes per 1/2gal water in a blender and chopped till fine bits.  Then I mixed with about 10 parts hpoo in a wheel barrow and dumped in the woods/bushes into a small level, but irregular mound and placed cardboard over it.

Will this work or would I have to use grain to make a slurry?  Or should I just stick with grain inoculation?  I have done grain slurries before with success in bulk corncob bits.


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Offlinejust me
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Re: Can you make agar slurry to inoculate outdoor hpoo? [Re: Buckeye Oysters]
    #14527449 - 05/28/11 06:04 PM (12 years, 8 months ago)

i tried a bed that was just lc, to a NICE susbstrate

it didnt fruit, or really colonize

spawn seems necessary, unless youre using spores.

ime...:shrug:


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InvisibleDoctor_Inoc
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Re: Can you make agar slurry to inoculate outdoor hpoo? [Re: Buckeye Oysters]
    #14527485 - 05/28/11 06:10 PM (12 years, 8 months ago)

Quote:

Buckeye Oysters said:
I had a few extra fully colonized dishes of agar that I put 3 dishes per 1/2gal water in a blender and chopped till fine bits.  Then I mixed with about 10 parts hpoo in a wheel barrow and dumped in the woods/bushes into a small level, but irregular mound and placed cardboard over it.

Will this work or would I have to use grain to make a slurry?  Or should I just stick with grain inoculation?  I have done grain slurries before with success in bulk corncob bits.



Contamination. 
(Unless you PC'ed your blender before making the slurry).
I would inoculate more petri dishes, checking for contamination, making the necassry transfers until a clean culture appeared..

Once a clean culture appeared, I would inoculate grains.
Once grains became fully colonized, I would then inoculate an outdoor bed of field capacity and pasteurized manure.

I wouldn't inoculate an out door bed of manure using live culture in liquid form.


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InvisibleDoc_T
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Re: Can you make agar slurry to inoculate outdoor hpoo? [Re: Doctor_Inoc]
    #14527542 - 05/28/11 06:20 PM (12 years, 8 months ago)

^^^ sounds about right.

Agar->grain->bulk is the standard method because it's the right way to do things.
Not to say it's impossible to innovate, but it's got to be something nobody has thought of yet.


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Offlinefaceyneck
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Re: Can you make agar slurry to inoculate outdoor hpoo? [Re: Buckeye Oysters]
    #14527543 - 05/28/11 06:21 PM (12 years, 8 months ago)

This I'm sure will work yes. The real rub is - how frequently will it yield mushrooms?

That I don't know. You'll need to try it out for yourself.

I don't see why there would be contamination problems, like another poster mentioned. Contamination is more of a monoculture (indoor) problem. Outdoors - things don't usually contaminate per se. However, all the other life floating around in the air and the :holyshit: might take over most of the time, meaning a low degree of success.

I say give it a solid try and see what happens. :super:


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Offlinejust me
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Re: Can you make agar slurry to inoculate outdoor hpoo? [Re: faceyneck]
    #14527618 - 05/28/11 06:39 PM (12 years, 8 months ago)

facey, that applies to some nice spawn and and some nice (pastuerized or not) substrate.

never saw a pro or con of either outdoors. suppose the pasteurized colonized better. but wetter. all depending tho (climate, waterings,etc)

but if youre using AGAR outdoors, its going to get some contam

and agar in the posters case is his spawn


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Re: Can you make agar slurry to inoculate outdoor hpoo? [Re: just me]
    #14527623 - 05/28/11 06:40 PM (12 years, 8 months ago)

Suppose you made the slurry (in a sterile blender) and then let it recolonize?
Like thick LC pudding...


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InvisibleDoctor_Inoc
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Re: Can you make agar slurry to inoculate outdoor hpoo? [Re: faceyneck]
    #14527665 - 05/28/11 06:49 PM (12 years, 8 months ago)

Quote:

I don't see why there would be contamination problems, like another poster mentioned.



2 reason:

1.  If the blender wasn't PC'ed before the slurry was made, it's not sterile.
2.  Once the slurry is introduced to the substrate materials outdoors, all the sugars from the agar are now through out the water, attracting every mold and bacteria spore under the sun to it like a tractor beam.

Inoculating outdoor substrates of cow manure or other manures cubensis grow from in the wild (not horse manure) with spores is know to succeed.


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OfflineBuckeye Oysters
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Re: Can you make agar slurry to inoculate outdoor hpoo? [Re: Doctor_Inoc]
    #14528003 - 05/28/11 08:06 PM (12 years, 8 months ago)

Quote:

Doctor_Inoc said:
Quote:

I don't see why there would be contamination problems, like another poster mentioned.



2 reason:

1.  If the blender wasn't PC'ed before the slurry was made, it's not sterile.
2.  Once the slurry is introduced to the substrate materials outdoors, all the sugars from the agar are now through out the water, attracting every mold and bacteria spore under the sun to it like a tractor beam.

Inoculating outdoor substrates of cow manure or other manures cubensis grow from in the wild (not horse manure) with spores is know to succeed.




Like I said the agar was fully colonized so it should have enough mycellium bits mixed homogeniously with the agar bits that it wont contam.  I am not colonizing the agar in the water, just chopping it up and immediately using it.  Outdoors you are not supposed to pasteurize or sterilize substrate as the natural bacteria and molds help you.

I'll update in a week.  I plan to make some with grain as well.


--------------------
Evolution is Lamarckism in disguise.  Adaptation never creates a new species or trait, but rather the new species/trait always existed within the parent DNA until circumstances allowed it to be activated.  For instance, every wolf has the DNA for poodles, but that DNA would never be revealed without man selectively breeding for it.


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Offlinefaceyneck
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Re: Can you make agar slurry to inoculate outdoor hpoo? [Re: Doctor_Inoc]
    #14528100 - 05/28/11 08:25 PM (12 years, 8 months ago)

Well, if the inoculation is taking place outdoors, I see no point in blending the slurry under sterile conditions. :shrug:

I doubt the agar pieces would be a problem outdoors. Like you said, EVERYTHING would be trying to grow on it, meaning not one thing can take over, and it's the one thing taking over which contaminates.

I also think this has likely been attempted before, but I haven't come across it yet. I'm interested to see what happens. :popcorn:


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Offlinefaceyneck
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Re: Can you make agar slurry to inoculate outdoor hpoo? [Re: Buckeye Oysters]
    #14528112 - 05/28/11 08:28 PM (12 years, 8 months ago)

Quote:

Buckeye Oysters said:
Quote:

Doctor_Inoc said:
Quote:

I don't see why there would be contamination problems, like another poster mentioned.



2 reason:

1.  If the blender wasn't PC'ed before the slurry was made, it's not sterile.
2.  Once the slurry is introduced to the substrate materials outdoors, all the sugars from the agar are now through out the water, attracting every mold and bacteria spore under the sun to it like a tractor beam.

Inoculating outdoor substrates of cow manure or other manures cubensis grow from in the wild (not horse manure) with spores is know to succeed.




Like I said the agar was fully colonized so it should have enough mycellium bits mixed homogeniously with the agar bits that it wont contam.  I am not colonizing the agar in the water, just chopping it up and immediately using it.  Outdoors you are not supposed to pasteurize or sterilize substrate as the natural bacteria and molds help you.

I'll update in a week.  I plan to make some with grain as well.





In a lecture from Paul Stamets, he talks of using pasteurized manure outdoors. Pasteurizing preserves the beneficial bacteria, so that's not a problem. Depending on the starting size of the manure pile, it'll oftentimes pasteurize itself.

Looking forward to the update. :super:


--------------------
Anything posted here, is total bullshit.
My Meyers-Briggs Personality: INTJ
New growers, or anyone else just needing help; I'm always glad to help right here. :grin:


We give cultivation advice here. :super:


AMU Q & A - We're glad to help
My Doggy Door Greenhouse!          First Ever Shmuvbox Tek!          Do Manure Right!


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InvisibleDoctor_Inoc
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Re: Can you make agar slurry to inoculate outdoor hpoo? [Re: Buckeye Oysters]
    #14529382 - 05/29/11 03:40 AM (12 years, 8 months ago)

Quote:

Like I said the agar was fully colonized so it should have enough mycellium bits mixed homogeniously with the agar bits that it wont contam.



Don't think so.
Culture on agar grows 2D on the surface of the medium.
Agar doesn't become "fully colonized" through the entire medium, or third-dimensionally.

Quote:

Outdoors you are not supposed to pasteurize or sterilize substrate as the natural bacteria and molds help you.



You are right about this. 
It is best to keep the wild microbes when cultivating outdoors.

Sterilizing or pasteurizing your materials for outdoor cultivation will result in whatever competitor microbe is the strongest, colonizing your substrate.
(chances are, it won't be the mushroom mycelium).

Concern is, once you blended that agar and myce into a slurry, the nutes and sugars were released into the slurry.
Any and every outdoor competitor and beneficial microbe spore will be drawn to that slurry like flies to shit.
That increases the chances of alpha-competitor spores taking a foot-holding onto the substrate.

Would'v planned on spawning grains to an outdoor bed of horse, cow, buffalo, elephant, goat, sheep dung ect..
Possibly buffering it up with some coir or vermicultie at low ratios (10-20%) and gypsum at 5%.
Or, inoculate an outdoor bed of cow manure with multi spore solution, or both.
Seeing as how you'v already concocted the slurry, of coarse give it a go and post results.


Edited by Doctor_Inoc (05/29/11 03:49 AM)


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InvisibleDoc_T
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Re: Can you make agar slurry to inoculate outdoor hpoo? [Re: Doctor_Inoc]
    #14529522 - 05/29/11 05:57 AM (12 years, 8 months ago)

You asked the question and got an answer.

Try it if you want.


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