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InvisibleNumeroEno
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Grey]
    #21834090 - 06/20/15 09:27 PM (8 years, 7 months ago)

I'm just picturing a cube cap shaped like a squid. You know, like real pointy and narrow.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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InvisibleInocuole
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Grey]
    #21834462 - 06/20/15 10:45 PM (8 years, 7 months ago)

Quote:

Grey said:
I'm talking about that Ganj's too. Which one you talking about ROBO?




I figured he was talking about yours.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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OfflineTravelAgency
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Re: Strain Thread-Strain Discussion Here Only Please [Re: eatyualive]
    #21834885 - 06/21/15 12:23 AM (8 years, 7 months ago)

No it was my first grow, a kit from MJ- about 6 years ago- from being a kit it was seriously prolific, using an hpoo, and I think coir, peat sub, (probably verm) pre sterilized, and I didn't buy it online, either; it was from a local head shop. I think he might have personally dropped it by because I never saw anything like it before or since- part of the directions actually included a (now outdated) sketch for an FC- I had several amazing experiences but they were very different- I wish I had taken spore prints- but a lot of horrible life shit happened inbetween then and now so there wouldn't even have been a way for me to keep them had I printed them. My coming back here is a part of my rebirth. I know MJ said it was a Thailand strain. First flush was recommended to leave in bag (with micro pore filter, big one) and then to birth to FC (pre shot gun tek) and Harvest first fruits. I didn't have a single 1/4 inch not pinning and producing baddies. This was even before cell phone cams were worth a shit so I don't even have any pics. But it was that kit that made me want to learn and do everything mycological.


--------------------





Indoor Greenhouse Build 
          Mid Size Mushroom Farm


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OfflineFranco Hamman
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Re: Strain Thread-Strain Discussion Here Only Please [Re: TravelAgency]
    #21840344 - 06/22/15 01:33 AM (8 years, 7 months ago)

Need some advice please, inoculated some popcorn with a syringe Burma strain.

It is winter at the moment and temps are ranging from 16 - 18 deg. Celsius (room temp). It has been a week since inoculation and no growth. I have a heating pad, could I use that for rising the temp so that the myc growth shall start?

First time with Burma, have no clue what to expect...


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Franco Hamman]
    #21840347 - 06/22/15 01:34 AM (8 years, 7 months ago)

Don't use the heating pad. Keep waiting. Spores can take 3 weeks or more to germinate sometimes. Start worrying in another week or so.

Pictures of your jars? How did you prep the popcorn? Hopefully there is enough moisture in them for the spores to germinate. Why are you using popcorn? There are better grains if you can find them, like wild bird seed, oats, rye.

Quote:

First time with Burma, have no clue what to expect...



You can expect them to act like every other variety.


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OfflineFranco Hamman
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Re: Strain Thread-Strain Discussion Here Only Please [Re: TheEaglesGift]
    #21840714 - 06/22/15 05:56 AM (8 years, 7 months ago)

I rinsed the corn and strained a couple of times, soaked over night and PC'd for an hour. I have used corn in the past with the least contam and wbs. You are right, there are plenty other grains which are better. What would you suggest? I am thinking of going back to wbs...

You mentioned oats, what type, prep etc? Also wbs, some advice on prepping the grain as good as can get? Sohr gum also work pretty well from what I have heard...?


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OfflineFranco Hamman
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Franco Hamman]
    #21841330 - 06/22/15 11:15 AM (8 years, 7 months ago)

I want to start cloning some fruits to agar and isolate the genes. What are some good signs to look out for when choosing a fruit? Size, density, colour...?


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InvisiblePastywhyteMDiscord
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Franco Hamman]
    #21841333 - 06/22/15 11:16 AM (8 years, 7 months ago)

Clustering.


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Strain Thread-Strain Discussion Here Only Please [Re: Franco Hamman]
    #21841337 - 06/22/15 11:18 AM (8 years, 7 months ago)

Try picking a fat pin within a cluster. Whatever's fastest growing. Transfer once, and use that plate to expand the culture as well as knocking up grain. You'll want to fruit a clone at least a few times to make sure it performs well.

You should take at least a few or so pins for clones, and fruit them on a small scale. Pick whatever performs the best and grow that culture for a long time


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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Invisible13shroomsM
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Buck513]
    #21841549 - 06/22/15 12:40 PM (8 years, 7 months ago)

:awesome2:


--------------------
:takingnotes:A M U:helpdesk:
Click here ^ for the AMU forum
VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV
"Man is the sex organ of the machine world" ~  Marshall McLuhan


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Franco Hamman]
    #21841884 - 06/22/15 02:37 PM (8 years, 7 months ago)

Quote:

Franco Hamman said:
I rinsed the corn and strained a couple of times, soaked over night and PC'd for an hour. I have used corn in the past with the least contam and wbs. You are right, there are plenty other grains which are better. What would you suggest? I am thinking of going back to wbs...

You mentioned oats, what type, prep etc? Also wbs, some advice on prepping the grain as good as can get? Sohr gum also work pretty well from what I have heard...?




I suggest oats or wbs. I prefer oats these days. I don't soak my oats or wbs(my wbs isn't very dirty, you might have to rinse), I just simmer or boil til they're done. This is tricky, takes some practice. There is no set time, you simmer them til they're hydrated but not to the point they start to burst. WBS will burst on you if you over cook it and don't stir it enough, but oats are rugged. Good luck getting oats to burst from over cooking.

Strain while steaming hot, don't rinse them. Let the steam dry them as you toss them around in the strainer. Takes some practice, but over the years this is the method I've gravitated towards. Soaking is pointless to me, I see zero benefit(and I've yet to see any conclusive evidence that soaking either helps or hurts in regards to endospores).

Jar up and pc for 120 minutes at 15 psi.


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OfflineMachiavelliavore
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Re: Strain Thread-Strain Discussion Here Only Please [Re: TheEaglesGift]
    #21842716 - 06/22/15 06:32 PM (8 years, 7 months ago)

It's interesting that so many people present the processs of drying grains as extended straining.

The best way to get grains to dry easiy is, IMO:

Remove from very hot water to increase evaporation

After straing for a short period, increase the surface area as much as possible (tote lids)

Sunlight and Air Flow:  Outside on a tote lid in the sun grains dry really fast.  heater/fan can also work, but not as well.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Machiavelliavore]
    #21842735 - 06/22/15 06:37 PM (8 years, 7 months ago)

I just rinse mine with hot tap water and spread them out on a towel.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Strain Thread-Strain Discussion Here Only Please [Re: Machiavelliavore]
    #21842737 - 06/22/15 06:37 PM (8 years, 7 months ago)

Quote:

It's interesting that so many people present the processs of drying grains as extended straining.

The best way to get grains to dry easiy is, IMO:

Remove from very hot water to increase evaporation

After straing for a short period, increase the surface area as much as possible (tote lids)

Sunlight and Air Flow:  Outside on a tote lid in the sun grains dry really fast.  heater/fan can also work, but not as well.




I actually don't dry my grains all that much. All my grain gets inocualted with wedges or g2g xfers, so I don't mind if my grain is a little on the wet side.

I also use 3/4 inch GE holes with entire CFDs under the lid. My jars get way more GE than most folks. If I prep grain as most others would, the tops of my jars dry out pretty badly.

I just dump my hots grains/water int he strainer and then jar it up once no more drips out the bottom. I might toss it around a few times. No offense, but you're doing a lot more to prep your grains than I am.


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OfflineMachiavelliavore
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Re: Strain Thread-Strain Discussion Here Only Please [Re: TheEaglesGift]
    #21842776 - 06/22/15 06:47 PM (8 years, 7 months ago)

I usually do the same.  My prep is typically something like tossing the grain in 4x boiling water, returning it to a boil, letting it sit for an hour, and straining it.

Sometimes I fuck up and end up with wet grain that I need to dry (esp grass seed) so those are some methods for fixing it.  I just find it odd when I see things like "strain overnight" that are pretty ineffective by comparison.

Wet grass seed also has a tendency to clump.  Grass seed is pretty annoying when done in quart glass jars tbh, and superdrying the grain definitely helped prevent some clumping.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Machiavelliavore]
    #21842806 - 06/22/15 06:53 PM (8 years, 7 months ago)

I just had my first problem from grains being too dry. I think I left them on the towel for too long. They were colonizing fine and then they stalled. I smelled them and they smelled like fresh cubes. Apparently they still colonized pretty fully, because I dunked and rinsed the grains from 2 of the jars yesterday and put them back in the jars. They're recovering really nicely, so I'm cautiously optimistic considering I was about to flush them. I think there's a reasonable chance that it will work, and I had nothing to lose by trying.

I really underestimated the effect the dry heat would have on my grows. It's consistently getting into the triple digits and the RH hasn't gotten above 10% in weeks.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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Offlineralph larun
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Re: Strain Thread-Strain Discussion Here Only Please [Re: NumeroEno]
    #21843116 - 06/22/15 07:57 PM (8 years, 7 months ago)

Ps. Subaeruginosa dose anyone know about this strain I know its a wood loving strain from ausrtilia but has any one grown it or have they grow wood loving species indoors


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InvisiblePastywhyteMDiscord
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Re: Strain Thread-Strain Discussion Here Only Please [Re: ralph larun]
    #21843127 - 06/22/15 08:00 PM (8 years, 7 months ago)

Wood loving species are very difficult to grow indoors. Some have done it, most fail. . .


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InvisibleNumeroEno
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Pastywhyte]
    #21843200 - 06/22/15 08:14 PM (8 years, 7 months ago)

That's a species, not a strain. I think there was someone who grew them indoors, but woodlovers in the cyanescens complex really prefer to grow outdoors.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Strain Thread-Strain Discussion Here Only Please [Re: NumeroEno]
    #21843475 - 06/22/15 09:10 PM (8 years, 7 months ago)

Quote:

NumeroEno said:
I just had my first problem from grains being too dry. I think I left them on the towel for too long. They were colonizing fine and then they stalled. I smelled them and they smelled like fresh cubes. Apparently they still colonized pretty fully, because I dunked and rinsed the grains from 2 of the jars yesterday and put them back in the jars. They're recovering really nicely, so I'm cautiously optimistic considering I was about to flush them. I think there's a reasonable chance that it will work, and I had nothing to lose by trying.

I really underestimated the effect the dry heat would have on my grows. It's consistently getting into the triple digits and the RH hasn't gotten above 10% in weeks.




Why did you put it back in the jars? Why not just rinse the uncolonized grain off and spawn? I'm interested in what you did, but a little confused about it.


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