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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: PrimalSoup]
#20942472 - 12/07/14 02:19 PM (9 years, 1 month ago) |
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Who cares if you are using agar though? I think agar is easier than making syringes anyways, and obviously safer. No pour MEA is as easy as it gets. I don't see the point of dicking around with syringes, other than for LC.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: d0urd3n]
#20942731 - 12/07/14 03:07 PM (9 years, 1 month ago) |
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I'm not talking about the agar work. Because you have to swab the gills to get spores, the swab has more potential for contamination than a print onto sterile media. When I went to test the swabs I got a lot of bacteria germination, but after a few weeks it also yielded mycelium. If I inoculated from spores on agar the bacteria load is minimal.
The vendors who make syringes with PE (at least in the case of sporeworks) centrifuge (sterile?) caps to separate the spores out. This was described in detail somewhere...
PE will rarely if ever drop spores voluntarily. How you gonna make a spore print then?
Could be I'm misunderstanding what you're trying to say though. 
PS
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: PrimalSoup]
#20942783 - 12/07/14 03:17 PM (9 years, 1 month ago) |
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Quote:
PrimalSoup said: Could be I'm misunderstanding what you're trying to say though. 
PS
Most likely my lack of a comma threw you off. I get what you are saying though. Bacteria on gills. Wonder if that is super common, or you just had a bacterial bunch for some reason. It just doesn't seem to be a huge deal to transfer until clean. Oh well. Thanks PS.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: d0urd3n]
#20942804 - 12/07/14 03:22 PM (9 years, 1 month ago) |
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No, it's not a contam on the fruit.
If you grow mushrooms outside of sterile conditions all parts of the fruit collect bacteria. When you print a cap under good conditions onto a sterile media there's only spores falling off the gills because the spores are actually launched by the spore-bearing structure into the air and descend. Bacteria spores just stay where they were under those conditions. But a swab picks up everything else along with spores.
PS
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: PrimalSoup]
#20942841 - 12/07/14 03:30 PM (9 years, 1 month ago) |
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Yeah fair enough I understand the fruits aren't ever that clean unless grown invitro. Still don't see why its a big deal to just clean it up on agar.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: d0urd3n]
#20943689 - 12/07/14 06:10 PM (9 years, 1 month ago) |
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Skills I suppose. 
PS
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: d0urd3n]
#20943691 - 12/07/14 06:10 PM (9 years, 1 month ago) |
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Quote:
d0urd3n said: Yeah fair enough I understand the fruits aren't ever that clean unless grown invitro. Still don't see why its a big deal to just clean it up on agar. 
With antibiotic agar and several different plates, you should be able to get it clean in just a transfer or two. Could always try the agar sandwich or something.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,070
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: PrimalSoup]
#20947518 - 12/08/14 01:23 PM (9 years, 1 month ago) |
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Quote:
PrimalSoup said: No. I've done it and given them away. But you've got a higher risk of contams that way, I think. 
PS
I love pre sterilized swabs.
There is a higher risk of contamination if you swab the gills directly.
However if you take a clean print first and swab the spores from the print you can be cleaner I think.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: elasticaltiger]
#20947592 - 12/08/14 01:43 PM (9 years, 1 month ago) |
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If I can't take a print I'll use swabs. I cut q-tips in half, wrap them in foil, and sterilize them first. 
PS
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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Fractal420
Psycellium



Registered: 06/21/13
Posts: 5,913
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: PrimalSoup]
#20956276 - 12/10/14 07:04 AM (9 years, 1 month ago) |
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Nothing is wrong with PE6 it's just a different strain.
-------------------- Dreaming of That face again. It's bright and blue and shimmering. Grinning wide And comforting me with it's three warm and wild eyes. Prying open MY third eye
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: Fractal420]
#20957036 - 12/10/14 11:47 AM (9 years, 1 month ago) |
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Quote:
Fractal420 said: Nothing is wrong with PE6 it's just a different strain.
Nothing is wrong with it, unless it's not what he wanted. If you expected it to be PE and it was pe6 then you would effectively have the wrong thing. It's just as good as any other cube, perhaps with a possibility of being a bit better, but it's still not good ol PE.
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Fractal420
Psycellium



Registered: 06/21/13
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: Inocuole]
#20957058 - 12/10/14 11:55 AM (9 years, 1 month ago) |
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So I thought I asked this before but I can't find it or maybe I never actually submitted the question, or maybe no one answered,
It was about PE2, 3, 4 and 5. What's up with those and why is it usually just PE, the albinos (different versions on SW as well) and then PE6. What is PE2?
-------------------- Dreaming of That face again. It's bright and blue and shimmering. Grinning wide And comforting me with it's three warm and wild eyes. Prying open MY third eye
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: Fractal420]
#20957087 - 12/10/14 12:01 PM (9 years, 1 month ago) |
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Wouldn't the others just be the failed crosses that took place prior to 6? I have no idea, just would make sense.
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Fractal420
Psycellium



Registered: 06/21/13
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: Inocuole]
#20957114 - 12/10/14 12:08 PM (9 years, 1 month ago) |
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^yeah, but then PE6 should be PE2, and I wonder what the other fruits turned out to be. I've seen a pic or two with people who apparently have all of them in print form (I recall at least one shroomerite posting a sig like that, a little while ago)
-------------------- Dreaming of That face again. It's bright and blue and shimmering. Grinning wide And comforting me with it's three warm and wild eyes. Prying open MY third eye
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: Fractal420]
#20957123 - 12/10/14 12:11 PM (9 years, 1 month ago) |
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Well PE and APE prints aren't impossible, just rare. I personally wouldn't want to decap(itate) all my PEs just to try to get the odd print.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: Fractal420]
#20957336 - 12/10/14 01:16 PM (9 years, 1 month ago) |
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Quote:
Fractal420 said: It was about PE2, 3, 4 and 5.
Just like WD37, WD38, and WD39. You don't see it. 
PS
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: Inocuole]
#20958152 - 12/10/14 04:14 PM (9 years, 1 month ago) |
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Quote:
Inocuole said: Well PE and APE prints aren't impossible, just rare. I personally wouldn't want to decap(itate) all my PEs just to try to get the odd print.
I'm curious as to how to harvest spores from my PEU. The mushroom that I cloned produced spores so hopefully my clone grows will produce spores too. I know I can take gill swabs but I'm really curious if I can make syringes somehow.
--------------------
Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: NumeroEno]
#20958280 - 12/10/14 04:33 PM (9 years, 1 month ago) |
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Quote:
EnoRoxxx said:
Quote:
Inocuole said: Well PE and APE prints aren't impossible, just rare. I personally wouldn't want to decap(itate) all my PEs just to try to get the odd print.
I'm curious as to how to harvest spores from my PEU. The mushroom that I cloned produced spores so hopefully my clone grows will produce spores too. I know I can take gill swabs but I'm really curious if I can make syringes somehow.
Learn agar man. It's a lot of fun and not too hard don't psych yourself out. Look up Doc_T's no pour agar tek that's the easier no pour imo. Or PastyPlates are great as well. Then once you get down your technique you can start with isolates and clones. Wish I would had got into it sooner.
Alright so what do you guys think about KSSS? Some fruits seem tiny but I hear they are dense. Other grows I've seen look like the average cube. Seems a bit odd it almost makes me think some other strain got mislabel as KSSS and spread perhaps? Some say potency is quite a bit above average, others say they're usually average. Ofc MS is a big factor.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: d0urd3n]
#20958290 - 12/10/14 04:34 PM (9 years, 1 month ago) |
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Quote:
d0urd3n said:
Quote:
EnoRoxxx said:
Quote:
Inocuole said: Well PE and APE prints aren't impossible, just rare. I personally wouldn't want to decap(itate) all my PEs just to try to get the odd print.
I'm curious as to how to harvest spores from my PEU. The mushroom that I cloned produced spores so hopefully my clone grows will produce spores too. I know I can take gill swabs but I'm really curious if I can make syringes somehow.
Learn agar man. It's a lot of fun and not too hard don't psych yourself out. Look up Doc_T's no pour agar tek that's the easier no pour imo. Or PastyPlates are great as well. Then once you get down your technique you can start with isolates and clones. Wish I would had got into it sooner.
Alright so what do you guys think about KSSS? Some fruits seem tiny but I hear they are dense. Other grows I've seen look like the average cube. Seems a bit odd it almost makes me think some other strain got mislabel as KSSS and spread perhaps? Some say potency is quite a bit above average, others say they're usually average. Ofc MS is a big factor.
I've been working with agar for a few months. I love it!
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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blackout


Registered: 07/16/00
Posts: 5,266
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Re: Strain Thread-Strain Discussion HERE ONLY Please [Re: Fractal420]
#20980091 - 12/15/14 03:12 PM (9 years, 1 month ago) |
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Quote:
Fractal420 said: What is PE2?
Quote:
Here’s how RogerRabbit says PE 6 came to be. “A single sector fruiting isolate of PE, and another of TEX were placed on a petri dish with rattlesnake venom added to the agar. The snake venom helped the two dikaryotic strains share genetic information to form a third sector, which was which was labeled PE6. Other Isolates of the PE were labeled PE 1-7, and apparently, some of the 6, which was the cross, were in the same batch of prints. The idea was to get a good spore producing PE, but I never had time to stabilize the strain due to accidentally releasing it early. That’s why sometimes they look like texans and sometimes very much like PE.”
Sporeworks had "atlantis #7" and "atlantis #3" for similar reasons, now listed as Psilocybe galindoi (galindii) var. Georgia ATL#7
I see they no longer have ATL#3.
As PE is so in demand and so hard to print from I always found it strange that live cultures were not more popular, where legal.
I remember getting several "agar bags" years ago when it was legal for me. They still sell sclerotia but never in tiny amounts which is all you would need to grow from, i.e. selling doses.
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