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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Strain Thread-Strain Discussion Here Only Please [Re: TheGuyNamedPuck]
#20573040 - 09/15/14 09:47 PM (9 years, 4 months ago) |
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AA+, CRS, PE, PF Albino. Those will serve you best as you can easily trade for the others with those IMO. Matter a fact I will trade you for the PF Albino if you get prints
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Pastywhyte]
#20573187 - 09/15/14 10:20 PM (9 years, 4 months ago) |
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Quote:
Pastywhyte said: AA+, CRS, PE, PF Albino. Those will serve you best as you can easily trade for the others with those IMO. Matter a fact I will trade you for the PF Albino if you get prints 
 
PS
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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starsky7
The Sponge



Registered: 09/12/14
Posts: 860
Loc: Eastern coast, USA
Last seen: 7 years, 8 months
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Pastywhyte]
#20574093 - 09/16/14 06:44 AM (9 years, 4 months ago) |
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Quote:
Pastywhyte said:
Quote:
Stevea1013 said:
Quote:
starsky7 said: I'm planning on colonizing 5 BRF jars, pre-sterilized until the pressure cooker arrives, then I'm going to be inoculating 5 quart sized wild bird seed after acquiring stable genetics from agar transfers. I already have everything set up and clean. I do however need to work on making more fruiting chamber. I'm considering a 4 tier greenhouse set up, but I'm going to wait for a few harvests first.
If your fairly new to cultivation I wouldn't worry about that do the pf tek take the bigger fruit, cut tissue from the inside of the lower portion of the stem and place it in the grain jar, tht tissue will contain sub genetics
Horrble advice. Could it work? Sure but I can also win the lottery. You want to stabilize genetics learn agar. After a year with success with agar you might have developed your procedure enough to get away with something like that. Except at that point you will better understand the pitfalls and you won't bother.
Okay, so, I had planned on using agar to stabilize the genetics. I'm not understanding what you're saying. I need the year of success to accomplish what? Are you referring to the WBS jars, or the tek of transferring tissue into WBS jars? I understand this is a terrible idea due to the possible contamination and unknown variables. Agar is DEF the best way that i know of to create stable genetics. I have been researching for quite some time, but nothing can make up for actual experience. And thanks for the advice and time, it's well valued.
-------------------- "In nature, there are neither rewards nor punishments, there are consequences" - Robert Greene Ingersoll
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Re: Strain Thread-Strain Discussion Here Only Please [Re: starsky7]
#20574102 - 09/16/14 06:51 AM (9 years, 4 months ago) |
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Quote:
starsky7 said:
Quote:
Pastywhyte said:
Quote:
Stevea1013 said:
Quote:
starsky7 said: I'm planning on colonizing 5 BRF jars, pre-sterilized until the pressure cooker arrives, then I'm going to be inoculating 5 quart sized wild bird seed after acquiring stable genetics from agar transfers. I already have everything set up and clean. I do however need to work on making more fruiting chamber. I'm considering a 4 tier greenhouse set up, but I'm going to wait for a few harvests first.
If your fairly new to cultivation I wouldn't worry about that do the pf tek take the bigger fruit, cut tissue from the inside of the lower portion of the stem and place it in the grain jar, tht tissue will contain sub genetics
Horrble advice. Could it work? Sure but I can also win the lottery. You want to stabilize genetics learn agar. After a year with success with agar you might have developed your procedure enough to get away with something like that. Except at that point you will better understand the pitfalls and you won't bother.
Okay, so, I had planned on using agar to stabilize the genetics. I'm not understanding what you're saying. I need the year of success to accomplish what? Are you referring to the WBS jars, or the tek of transferring tissue into WBS jars? I understand this is a terrible idea due to the possible contamination and unknown variables. Agar is DEF the best way that i know of to create stable genetics. I have been researching for quite some time, but nothing can make up for actual experience. And thanks for the advice and time, it's well valued.
I tried using the faht jars (basically a grain jar but with only about a 1/2" of grain in it) for cloning tissue, it's very hit or miss as you can't tell what's riding along with the tissue sample on grain like you can on agar.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Strain Thread-Strain Discussion Here Only Please [Re: starsky7]
#20574147 - 09/16/14 07:16 AM (9 years, 4 months ago) |
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Quote:
starsky7 said:
Quote:
Pastywhyte said:
Quote:
Stevea1013 said:
Quote:
starsky7 said: I'm planning on colonizing 5 BRF jars, pre-sterilized until the pressure cooker arrives, then I'm going to be inoculating 5 quart sized wild bird seed after acquiring stable genetics from agar transfers. I already have everything set up and clean. I do however need to work on making more fruiting chamber. I'm considering a 4 tier greenhouse set up, but I'm going to wait for a few harvests first.
If your fairly new to cultivation I wouldn't worry about that do the pf tek take the bigger fruit, cut tissue from the inside of the lower portion of the stem and place it in the grain jar, tht tissue will contain sub genetics
Horrble advice. Could it work? Sure but I can also win the lottery. You want to stabilize genetics learn agar. After a year with success with agar you might have developed your procedure enough to get away with something like that. Except at that point you will better understand the pitfalls and you won't bother.
Okay, so, I had planned on using agar to stabilize the genetics. I'm not understanding what you're saying. I need the year of success to accomplish what? Are you referring to the WBS jars, or the tek of transferring tissue into WBS jars? I understand this is a terrible idea due to the possible contamination and unknown variables. Agar is DEF the best way that i know of to create stable genetics. I have been researching for quite some time, but nothing can make up for actual experience. And thanks for the advice and time, it's well valued.
Read my post again. I was talking about putting a piece of a stem directly in a grain jar. Your plan to put it to agar is fine, in fact its how it should be done. After a year of taking clones properly on agar you will know why putting it straight to grain is a bad idea.
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Stevea1013



Registered: 07/27/14
Posts: 108
Loc: New york
Last seen: 8 years, 11 months
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Pastywhyte]
#20574863 - 09/16/14 11:05 AM (9 years, 4 months ago) |
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Ohok, my apology. Yes it deft isn't the most sterile or effective way to clone but it is simple and works, not 100% but more times than less. But if your going to skip the basics and jump into advanced mycology I would recomend using the method pastywhyte recommends
-------------------- freedom isn't free.... it is payed with the blood of fallen soldiers and on the backs of the ones still fighting the good fight! BRAVO 258 IN REG 1st PLATOON "WARRIORS"
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starsky7
The Sponge



Registered: 09/12/14
Posts: 860
Loc: Eastern coast, USA
Last seen: 7 years, 8 months
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Pastywhyte]
#20575477 - 09/16/14 02:14 PM (9 years, 4 months ago) |
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Quote:
Pastywhyte said:
Read my post again. I was talking about putting a piece of a stem directly in a grain jar. Your plan to put it to agar is fine, in fact its how it should be done. After a year of taking clones properly on agar you will know why putting it straight to grain is a bad idea.
Ahhh, I see. Yeah, considering that the fruiting stage is done in a partially open air environment, and at any given time, you are exposing them to tons of contaminants via fanning. So taking a piece of tissue and throwing it in the jar IS the lottery.
-------------------- "In nature, there are neither rewards nor punishments, there are consequences" - Robert Greene Ingersoll
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Stevea1013



Registered: 07/27/14
Posts: 108
Loc: New york
Last seen: 8 years, 11 months
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Re: Strain Thread-Strain Discussion Here Only Please [Re: starsky7]
#20575634 - 09/16/14 02:46 PM (9 years, 4 months ago) |
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Quote:
starsky7 said:
Quote:
Pastywhyte said:
Read my post again. I was talking about putting a piece of a stem directly in a grain jar. Your plan to put it to agar is fine, in fact its how it should be done. After a year of taking clones properly on agar you will know why putting it straight to grain is a bad idea.
Ahhh, I see. Yeah, considering that the fruiting stage is done in a partially open air environment, and at any given time, you are exposing them to tons of contaminants via fanning. So taking a piece of tissue and throwing it in the jar IS the lottery.
Tissue from the interior of the stem was what I ment
-------------------- freedom isn't free.... it is payed with the blood of fallen soldiers and on the backs of the ones still fighting the good fight! BRAVO 258 IN REG 1st PLATOON "WARRIORS"
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Stevea1013] 1
#20575647 - 09/16/14 02:49 PM (9 years, 4 months ago) |
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Quote:
Stevea1013 said: Ohok, my apology. Yes it deft isn't the most sterile or effective way to clone but it is simple and works, not 100% but more times than less.
I know of a guy who tried this 1 outta 10 jars made it. He used tissue from the inside of the stem. Anyone who has cloned using stem tissue knows how often it can contam. Like I said in my previous posts. Also for the record agar is not advanced mycology, my tek in my sig is as simple as the pf tek. If making jello is beyond you then okay stay away from agar
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starsky7
The Sponge



Registered: 09/12/14
Posts: 860
Loc: Eastern coast, USA
Last seen: 7 years, 8 months
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Pastywhyte]
#20575663 - 09/16/14 02:54 PM (9 years, 4 months ago) |
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Youch.
-------------------- "In nature, there are neither rewards nor punishments, there are consequences" - Robert Greene Ingersoll
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Stevea1013



Registered: 07/27/14
Posts: 108
Loc: New york
Last seen: 8 years, 11 months
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Pastywhyte]
#20575665 - 09/16/14 02:55 PM (9 years, 4 months ago) |
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Quote:
Pastywhyte said:
Quote:
Stevea1013 said: Ohok, my apology. Yes it deft isn't the most sterile or effective way to clone but it is simple and works, not 100% but more times than less.
I know of a guy who tried this 1 outta 10 jars made it. He used tissue from the inside of the stem. Anyone who has cloned using stem tissue knows how often it can contam. Like I said in my previous posts. Also for the record agar is not advanced mycology, my tek in my sig is as simple as the pf tek. If making jello is beyond you then okay stay away from agar 
I seen your hello tek I'm impressed! But don't you use a tissue sample to clone on agar?
-------------------- freedom isn't free.... it is payed with the blood of fallen soldiers and on the backs of the ones still fighting the good fight! BRAVO 258 IN REG 1st PLATOON "WARRIORS"
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Strain Thread-Strain Discussion Here Only Please [Re: starsky7]
#20575666 - 09/16/14 02:55 PM (9 years, 4 months ago) |
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and he was lucky to get that one to work
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Strain Thread-Strain Discussion Here Only Please [Re: cronicr]
#20575711 - 09/16/14 03:07 PM (9 years, 4 months ago) |
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Of course I put tissue on agar, thats the whole point but I don't put one in a grain jar cause I don't wanna grow a jar of bacteria.
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Stevea1013



Registered: 07/27/14
Posts: 108
Loc: New york
Last seen: 8 years, 11 months
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Pastywhyte]
#20575722 - 09/16/14 03:11 PM (9 years, 4 months ago) |
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Quote:
Pastywhyte said: Of course I put tissue on agar, thats the whole point but I don't put one in a grain jar cause I don't wanna grow a jar of bacteria.
Gotcha
-------------------- freedom isn't free.... it is payed with the blood of fallen soldiers and on the backs of the ones still fighting the good fight! BRAVO 258 IN REG 1st PLATOON "WARRIORS"
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starsky7
The Sponge



Registered: 09/12/14
Posts: 860
Loc: Eastern coast, USA
Last seen: 7 years, 8 months
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Pastywhyte]
#20575732 - 09/16/14 03:12 PM (9 years, 4 months ago) |
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Correct me if i'm wrong, but there are normally only 2 things you want to put on your substrate...
1: Spores 2: Living mycelium in the for of living culture
-------------------- "In nature, there are neither rewards nor punishments, there are consequences" - Robert Greene Ingersoll
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xanadu_poppy
Stranger

Registered: 08/04/14
Posts: 69
Last seen: 6 years, 1 month
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Re: Strain Thread-Strain Discussion Here Only Please [Re: TheGuyNamedPuck]
#20589708 - 09/19/14 06:18 PM (9 years, 4 months ago) |
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I really like golden teacher.
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Fractal420
Psycellium



Registered: 06/21/13
Posts: 5,913
Last seen: 8 months, 16 days
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Re: Strain Thread-Strain Discussion Here Only Please [Re: xanadu_poppy]
#20591834 - 09/20/14 07:33 AM (9 years, 4 months ago) |
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I have no idea how to make jello. I know I shouldn't even attempt working with agar.
I personally like using MS>g2g. Takes a little while longer, but I like the simplicity. Also it seems working with one type of spawn isn't too bad. Mixing a bunch of MS spawn in a tub can be a bit unpredictable but if I g2g from one pint they're all basically the same as far as I can tell
-------------------- Dreaming of That face again. It's bright and blue and shimmering. Grinning wide And comforting me with it's three warm and wild eyes. Prying open MY third eye
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Fractal420]
#20591945 - 09/20/14 08:40 AM (9 years, 4 months ago) |
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I did the MS syringe thing for a while, Actually most of my grows are still MS even though they all start with agar. The use of agar goes far beyond strain isolation. I don't think it is possible to be serious about this hobby without using agar. It is the foundation of everything I do.
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Dilated
KB Cubensis Fanaticus



Registered: 03/28/14
Posts: 2,347
Loc: The Ether
Last seen: 3 years, 2 months
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Fractal420]
#20591969 - 09/20/14 08:51 AM (9 years, 4 months ago) |
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Quote:
Fractal420 said: I have no idea how to make jello. I know I shouldn't even attempt working with agar.
I personally like using MS>g2g. Takes a little while longer, but I like the simplicity. Also it seems working with one type of spawn isn't too bad. Mixing a bunch of MS spawn in a tub can be a bit unpredictable but if I g2g from one pint they're all basically the same as far as I can tell
Making agar is easier then making jello. Especially if you check out some Pasty plates How do you know what you're squirting into your jar is contamination free? You don't....
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Dilated]
#20592021 - 09/20/14 09:08 AM (9 years, 4 months ago) |
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I poured a sleeve last night. It took almost no time or effort. I made dog food agar. I just put 10 grams of dog food and 10 grams of agar powder in a blender. ran it for a few second then added 500 ML of water and ran it again. Then I poured it into a pot, simmered it for a few minutes and poured it into a bottle to PC. PCed for 30 minutes and after it cooled enough to handle I spent all of 5 minutes pouring 20 plates in my SAB. It's really easy.
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