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jmoney757
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Registered: 04/17/13
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Loc: va
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Re: Strain Thread-Strain Discussion Here Only Please [Re: FrankHorrigan]
#18180178 - 04/28/13 06:58 AM (10 years, 8 months ago) |
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Yeah I hear u man I didnt know u could get that sent
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
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Re: Strain Thread-Strain Discussion Here Only Please [Re: jmoney757]
#18180940 - 04/28/13 11:49 AM (10 years, 8 months ago) |
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You can mail anything.
PS
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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mattsls101
Wanderer of Time



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Re: Strain Thread-Strain Discussion Here Only Please [Re: PrimalSoup]
#18180967 - 04/28/13 11:57 AM (10 years, 8 months ago) |
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Quilla has anyone every mess whit it looking for hobbit info, and development time. Does anyone know what it would look like???
-------------------- How it should & shouldn't look - NEW CULTIVATORS GUIDE *UPDATED* >>>>> http://www.shroomery.org/forums/showflat.php/Number/17231150/fpart/all/vc/1
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Javadog
Continuing along



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Posts: 7,385
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Re: Strain Thread-Strain Discussion Here Only Please [Re: mattsls101]
#18187104 - 04/29/13 03:12 PM (10 years, 8 months ago) |
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I know of a Quila strain of cubensis. Is this what you mean?
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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OoBYCoO
One grow down, a million to go!!



Registered: 08/18/10
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Javadog]
#18187156 - 04/29/13 03:21 PM (10 years, 8 months ago) |
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yup
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mike-ologist
appreciator of life



Registered: 11/28/12
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Sillyputty67]
#18187327 - 04/29/13 03:50 PM (10 years, 8 months ago) |
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Is this edible/active or is this purely active strains?
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
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Re: Strain Thread-Strain Discussion Here Only Please [Re: mike-ologist]
#18187403 - 04/29/13 04:07 PM (10 years, 8 months ago) |
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Quote:
Welcome to myCERVANTES Race/Variety/Strain Journal. This is where we discuss 'Strains'.
Please read any pertinent information in these first THREE posts before asking about any cube 'Strain'.
If you have already read the first three posts of this thread, start posting. If not, read on.
PS
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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Solipsis
m̶a̶d̶ disappointed scientist



Registered: 12/28/09
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Re: Strain Thread-Strain Discussion Here Only Please [Re: PrimalSoup]
#18214414 - 05/04/13 05:39 PM (10 years, 8 months ago) |
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In the future I would like to experiment with hybridization, but I realize that there are intermediate cultivation techniques to master first. I am only just using agar for example (though I have experience with it from college).
So before I try to make a hybrid I want to get familiar with the 2 strains I will eventually cross.
Apparently (according to Workman) it is very hard to spot a succesful crossing at first because first the fruits may inherit more typical traits and their offspring are the true hybrids (if you succeed). Therefore it seems best to use unusual looking Cubensis strains so that you can spot traits more easily. PE x Albino A+/Albino PF is a classic example.
To my question: what is another Cubensis strain besides PE and Albino's? Because it seems to me that these are the only ones that actually stand out. thanks
Edited by Solipsis (05/04/13 05:42 PM)
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fastfred
Old Hand



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Re: Strain Thread-Strain Discussion Here Only Please [Re: Solipsis]
#18214889 - 05/04/13 07:27 PM (10 years, 8 months ago) |
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Redspore.
If you isolate monokaryons it's not hard to verify crosses on the dishes.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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Solipsis
m̶a̶d̶ disappointed scientist



Registered: 12/28/09
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Re: Strain Thread-Strain Discussion Here Only Please [Re: fastfred]
#18214949 - 05/04/13 07:42 PM (10 years, 8 months ago) |
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I assume you mean the 'third' sectors..?
Thanks yes I guess redspore is another one that's unusual. For some reason a Redspore / Redboy x Albino makes me think of little red ridinghood.
Only thing I'm not sure of is if isolating monokaryons requires spore soln. dilution or picking off a spore by microscope, because typical ways of inoculation are multispore... I guess I must read up! edit: I see 'Workmans Breeding Method' describes a way to do this. Hmm funny, I thought that any myc visible to the naked eye would already be mated because of extremely close clumping.
Edited by Solipsis (05/04/13 07:44 PM)
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cronicr



Registered: 08/07/11
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Solipsis]
#18215281 - 05/04/13 09:02 PM (10 years, 8 months ago) |
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take a spore print of aa+ and and then print some redboy right on top, inoculate and fruit, clone the albino that drops redspores but hybridizing is totaly diffrent from crossing, crossing is whats done between 2 varietys of the same species such as cubes, hybridizing is between 2 speciec like pans and cubes
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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fastfred
Old Hand



Registered: 05/17/04
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Loc: Dark side of the moon
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Re: Strain Thread-Strain Discussion Here Only Please [Re: cronicr]
#18215643 - 05/04/13 10:19 PM (10 years, 8 months ago) |
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> I assume you mean the 'third' sectors..?
When monokaryon myc meets you'll get a "line of inhibition", they'll grow together, or you'll get sectoring.
If it sectors or forms a raised/thickened line there's almost certainly a mating there. You can verify this by looking for clamp connections with a scope.
The techniques for isolating monokaryons aren't really all that difficult. You need well shaken spores with a surfactant (like JetDry, Tween, etc.) in the mix. That will reduce clumping. Then you just need the right dilution. From there take a micropipettor or simple dropper and adjust so that you average 1 spore per drop. Put some drops on a plate and scope them. Mark the ones that have only one spore and re-spot the empty drops and excise the multi-spore drops, or just keep track and transfer the good ones.
Even a crappy scope should be good enough for this sort of thing.
You could also just streak them out and pick the monokaryons before they mate.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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Solipsis
m̶a̶d̶ disappointed scientist



Registered: 12/28/09
Posts: 3,398
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Re: Strain Thread-Strain Discussion Here Only Please [Re: fastfred]
#18215678 - 05/04/13 10:28 PM (10 years, 8 months ago) |
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Both of you thanks very much.
I will bookmark this for future reference and might try both approaches at different stages, actually.
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Slugbird
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Registered: 04/21/13
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Solipsis]
#18217316 - 05/05/13 10:46 AM (10 years, 8 months ago) |
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I've heard that Cambodians, on average, tend to colonize faster than other cubes. Is this true?
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Slugbird]
#18217548 - 05/05/13 11:46 AM (10 years, 8 months ago) |
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Quite likely. All varieties, on average, tend to colonize faster than other cubes. It's the same as all the children being above average in Lake Wobegon. 
PS
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Re: Strain Thread-Strain Discussion Here Only Please [Re: PrimalSoup]
#18221648 - 05/06/13 10:02 AM (10 years, 8 months ago) |
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Quote:
PrimalSoup said: Quite likely. All varieties, on average, tend to colonize faster than other cubes. It's the same as all the children being above average in Lake Wobegon. 
PS
"Where all the women are strong and all the men are good looking."
Hmmmm....more like Lake Ibegone
:0)
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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newera
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Registered: 03/05/13
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Re: Strain Thread-Strain Discussion Here Only Please [Re: Javadog]
#18262196 - 05/14/13 04:15 AM (10 years, 8 months ago) |
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ok maybe a dumb question or the wrong place to put it but... i got some spores from an old hippie who said he had been growing them for years and taking his own prints, after a sad performance he tells me that he might be over using his variety, meaning growing/printing/growing/printing/growing/printing and tells me that over time, the result of this can be weakened genetics and it might be time for a fresh start. is there any truth to this or am i hanging out with a man whos done too much acid?
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ProfessorPinHead
Trapped in the Archives....




Registered: 07/09/10
Posts: 4,568
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Re: Strain Thread-Strain Discussion Here Only Please [Re: newera]
#18263079 - 05/14/13 10:06 AM (10 years, 8 months ago) |
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Quote:
is there any truth to this or am i hanging out with a man whos done too much acid?
This......... 
If it is coming from a print the genetics should be a fresh start and if the results are poor it is a genetic problem rather than a problem with senescence.
Cube varieties such as B+, EQ, PE, Malabar, Treasure Coast, etc don't get degraded over time but a strain will if it is an isolate and it is not properly backed up and kept in cold storage.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Strain Thread-Strain Discussion Here Only Please [Re: newera]
#18263103 - 05/14/13 10:13 AM (10 years, 8 months ago) |
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Quote:
newera said: hanging out with a man whos done too much acid?
That.
Each two spores that germinate and mate are creating a brand new set of genetics. I don't see how else you could have a fresher start.
Edit: PP you beat me to it, I did not see your post
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ProfessorPinHead
Trapped in the Archives....




Registered: 07/09/10
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Re: Strain Thread-Strain Discussion Here Only Please [Re: FrankHorrigan]
#18263136 - 05/14/13 10:23 AM (10 years, 8 months ago) |
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Quote:
FrankHorrigan said:
Edit: PP you beat me to it, I did not see your post 
I hate when that happens, 
It's good to know we are on the same train of thought Frank...
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