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quebus
Stranger

Registered: 05/01/10
Posts: 678
Last seen: 3 months, 24 days
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2 out of 5 contaminated - mono becomes a mini
#14521677 - 05/27/11 03:31 PM (12 years, 9 months ago) |
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The origins of this are PE MS spawned to WBS then a G2G WBS to rye. I went to do a mono tub using 5 quarts and spotted these two gems:

So - I am left with three that I will now spawn to a mini mono. I did the 90 minute PC at 15 PSI so the source must be the WBS. I have a mono tub colonizing now with the same WBS - we'll see how that turns out.
Here is my setup for fruiting with two mini's going. I just bought the 6400K bulbs. These is an oscillating fan in the room moving air around.

This is in my basement and I use a plant stand that I use to use to grow my flowers from seed. The light fixture is hanging on a removable piece of plywood.
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jazzaero
Still just a spore



Registered: 04/29/11
Posts: 91
Last seen: 8 years, 9 months
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: quebus]
#14521697 - 05/27/11 03:34 PM (12 years, 9 months ago) |
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Nice little setup you have there. 
Sorry bout the contam though.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: jazzaero]
#14521792 - 05/27/11 03:48 PM (12 years, 9 months ago) |
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make sure to PC those jars for 60 minutes before you dump your grain.
& I think you meant 6500K lights...
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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steelmonkey
Homejigger



Registered: 02/22/11
Posts: 1,822
Loc: Nova Scotia,Canada
Last seen: 2 years, 5 months
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: jazzaero]
#14521803 - 05/27/11 03:50 PM (12 years, 9 months ago) |
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Dude I feel for you that just sucks
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: steelmonkey]
#14521813 - 05/27/11 03:51 PM (12 years, 9 months ago) |
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I would've just G2G'd the three jars to 21 more jars & had 2 monos, but that's me...
& the source isn't the WBS, cuz you PCed @ 15 for 90...it's your sterile procedure during inoculation or your syringe. Remember no print is 100% sterile, so when you make an MS syringe from a print, you'll have a few contams in it.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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quebus
Stranger

Registered: 05/01/10
Posts: 678
Last seen: 3 months, 24 days
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: TranscendingLife]
#14522008 - 05/27/11 04:25 PM (12 years, 9 months ago) |
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I don't have 20 jars to G2G at the moment. I use a still air box and a flame etc. I will PC these two before emptying.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: quebus]
#14522046 - 05/27/11 04:31 PM (12 years, 9 months ago) |
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Quote:
phortre55 said: I don't have 20 jars to G2G at the moment.
That sux dude, you mean you don't have a cabinet that looks like this:

Guess it's time for ya to go buy some more
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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jazzaero
Still just a spore



Registered: 04/29/11
Posts: 91
Last seen: 8 years, 9 months
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: TranscendingLife]
#14522114 - 05/27/11 04:46 PM (12 years, 9 months ago) |
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Quote:
todlow said:
Quote:
phortre55 said: I don't have 20 jars to G2G at the moment.
That sux dude, you mean you don't have a cabinet that looks like this:

Guess it's time for ya to go buy some more 
Show off! 
I need to get a setup like that. lol
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glitche
Genetic Engineer

Registered: 04/07/11
Posts: 241
Last seen: 8 years, 3 months
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: TranscendingLife]
#14522216 - 05/27/11 05:05 PM (12 years, 9 months ago) |
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Where did you get the synthetic filter disc and rubber injection ports?
I'm thinking about buying some myself =)
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afrosheen
9Lives the cat



Registered: 03/06/10
Posts: 1,878
Last seen: 2 years, 5 months
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: glitche]
#14522378 - 05/27/11 05:40 PM (12 years, 9 months ago) |
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Quote:
& the source isn't the WBS, cuz you PCed @ 15 for 90...it's your sterile procedure during inoculation
Yeah, the source is the WBS. He said he did g2g for these jars, so contams snuck in during the g2g process most likely. Lid tek could be bad also, can't see them.
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quebus
Stranger

Registered: 05/01/10
Posts: 678
Last seen: 3 months, 24 days
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: afrosheen]
#14523763 - 05/27/11 10:04 PM (12 years, 9 months ago) |
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Quote:
afrosheen said:
Quote:
& the source isn't the WBS, cuz you PCed @ 15 for 90...it's your sterile procedure during inoculation
Yeah, the source is the WBS. He said he did g2g for these jars, so contams snuck in during the g2g process most likely. Lid tek could be bad also, can't see them.
Lid tek is RTV injection port with synthetic filter disc and 4 FAE holes. N doubt the still air box was the source. I use tyvek sleeves and gloves...
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: glitche]
#14523962 - 05/27/11 10:43 PM (12 years, 9 months ago) |
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yea, sterile procedure during G2G was definitely the source. IDK how though, if you did 1 jar to 5...my 1 jar to 5 jars colonize in 9 days vs. 1 jar to 10 jars, which is 16...
Quote:
glitche said:

Where did you get the synthetic filter disc and rubber injection ports?
I'm thinking about buying some myself =)
That's red RTV silicone as an injection port (see lid tek in sig) SFD's can be purchased from everythingmushrooms.com @ 12 100mm disks for $12.
OP, try drilling 3/8"-1/2" holes in your lids & silicone them down (like my tek) instead of just using a whole SFD per lid. It'll stretch your dollar & may help w/ contams, due to the lid ring not having to pinch the SFD under it.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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bootster


Registered: 02/22/11
Posts: 1,531
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: TranscendingLife]
#14524257 - 05/27/11 11:45 PM (12 years, 9 months ago) |
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I just noticed that you went from circles to squares on your lids. Why not get yourself one of these:
Gasket Punch
I have a Blue Point set (Snap On)that someone gave me 20 yrs. ago and it makes the circles perfect and accurate every time. Proper tool for the job.
Nice cabinet BTW.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: bootster]
#14524326 - 05/27/11 11:56 PM (12 years, 9 months ago) |
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thanks on the cabinet. I agree w/ the "proper tool for the proper job," but I'm not about to go spend $ when I can just use scissors & add 30 seconds to the process...Besides, once the SFDs are cut they'll last years....where as the lids, depending on use/abuse, will last a year or so...
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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bootster


Registered: 02/22/11
Posts: 1,531
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: TranscendingLife]
#14524360 - 05/28/11 12:02 AM (12 years, 9 months ago) |
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I agree. Couldn't you just cut the SFDs off the trashed lids with a razor knife and reuse them on new lids? I cut 30 circles (I use 7/8") in about 3 min. with my punch and they really do look "non ghetto".
I am just figuring you out to be a guy who would appreciate looking at the perfect circles as many times as you look at those lids/jars. I do!
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: bootster]
#14524390 - 05/28/11 12:08 AM (12 years, 9 months ago) |
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yea, I'm anal as shit, but IDC about my jar lids' aesthetics as long as they work.
You're completely correct, you can just use a razor knife to cut the silicone to free the SFD & move it to a new lid. Actually, I state that in my lid tek...
back to the topic...
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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glitche
Genetic Engineer


Registered: 04/07/11
Posts: 241
Last seen: 8 years, 3 months
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Re: 2 out of 5 contaminated - mono becomes a mini [Re: TranscendingLife]
#14525972 - 05/28/11 11:33 AM (12 years, 9 months ago) |
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That's red RTV silicone as an injection port (see lid tek in sig) SFD's can be purchased from everythingmushrooms.com @ 12 100mm disks for $12.
OP, try drilling 3/8"-1/2" holes in your lids & silicone them down (like my tek) instead of just using a whole SFD per lid. It'll stretch your dollar & may help w/ contams, due to the lid ring not having to pinch the SFD under it.
I appreciate it man! Thanks
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